st061468

5,7-dimethoxy-4-methylchromen-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1548
Screen concentration 90.8 μM
Source TimTec (Pure natural product library)
PubChem CID 390799
SMILES CC1=CC(=O)OC2=CC(=CC(=C12)OC)OC
Standardized SMILES COc1cc(OC)c2C(=CC(=O)Oc2c1)C
Molecular weight 220.2213
ALogP 2.31
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.61
% growth inhibition (Hom. pool) 1.31


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 390799
Download HIP data (tab-delimited text)  (excel)
Gene:ERG11(YHR007C)|FD-Score:-3.93|P-value:4.28E-5|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:RLP24(YLR009W)|FD-Score:-3.49|P-value:2.39E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:TIF35(YDR429C)|FD-Score:-3.24|P-value:5.99E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:ERG11(YHR007C)|FD-Score:-3.93|P-value:4.28E-5|Clearance:0||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:RLP24(YLR009W)|FD-Score:-3.49|P-value:2.39E-4|Clearance:0||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:TIF35(YDR429C)|FD-Score:-3.24|P-value:5.99E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 390799
Download HOP data (tab-delimited text)  (excel)
Gene:ALD6(YPL061W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:COG7(YGL005C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX11(YPL132W)|FD-Score:-3.76|P-value:8.60E-5||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:GCN2(YDR283C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:IMD3(YLR432W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IRC14(YOR135C_d)|FD-Score:4.44|P-value:4.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRS4(YKR019C)|FD-Score:4.35|P-value:6.91E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ITR2(YOL103W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:LSM7(YNL147W)|FD-Score:4.84|P-value:6.39E-7||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MDH3(YDL078C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle Gene:MSC1(YML128C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:NDE1(YMR145C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NHA1(YLR138W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:RPL21A(YBR191W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:STE24(YJR117W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SVF1(YDR346C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TAT1(YBR069C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THI7(YLR237W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:TRP1(YDR007W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.8|P-value:3.30E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.61|P-value:1.39E-14||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.59|P-value:2.27E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBX6(YJL048C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBR027C(YBR027C_d)|FD-Score:4.37|P-value:6.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR063C(YBR063C_p)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative protein of unknown function; YBR063C is not an essential gene Gene:YBR285W(YBR285W_p)|FD-Score:3.85|P-value:5.97E-5||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YDR010C(YDR010C_d)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL118C(YGL118C_d)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL242C(YGL242C_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR026W(YGR026W_p)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGR039W(YGR039W_d)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 Gene:YJL045W(YJL045W)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YKL096C-B(YKL096C-B_p)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR063C(YPR063C_p)|FD-Score:-3.11|P-value:9.41E-4||SGD DESC:ER-localized protein of unknown function Gene:ALD6(YPL061W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:COG7(YGL005C)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX11(YPL132W)|FD-Score:-3.76|P-value:8.60E-5||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:GCN2(YDR283C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:IMD3(YLR432W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IRC14(YOR135C_d)|FD-Score:4.44|P-value:4.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRS4(YKR019C)|FD-Score:4.35|P-value:6.91E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ITR2(YOL103W)|FD-Score:3.44|P-value:2.89E-4||SGD DESC:Myo-inositol transporter with strong similarity to the major myo-inositol transporter Itr1p, member of the sugar transporter superfamily; expressed constitutively Gene:LSM7(YNL147W)|FD-Score:4.84|P-value:6.39E-7||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MDH3(YDL078C)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Peroxisomal malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle Gene:MSC1(YML128C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated Gene:NDE1(YMR145C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Mitochondrial external NADH dehydrogenase, a type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain Gene:NHA1(YLR138W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:RPL21A(YBR191W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPL22A(YLR061W)|FD-Score:-3.2|P-value:6.98E-4||SGD DESC:Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication Gene:STE24(YJR117W)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans Gene:SVF1(YDR346C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TAT1(YBR069C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THI7(YLR237W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia Gene:TRP1(YDR007W)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:5.8|P-value:3.30E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.61|P-value:1.39E-14||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:4.59|P-value:2.27E-6||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBX6(YJL048C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:VPS1(YKR001C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YBR027C(YBR027C_d)|FD-Score:4.37|P-value:6.22E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR063C(YBR063C_p)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative protein of unknown function; YBR063C is not an essential gene Gene:YBR285W(YBR285W_p)|FD-Score:3.85|P-value:5.97E-5||SGD DESC:Putative protein of unknown function; YBR285W is not an essential gene Gene:YDR010C(YDR010C_d)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL118C(YGL118C_d)|FD-Score:3.99|P-value:3.34E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL242C(YGL242C_p)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YGR026W(YGR026W_p)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGR039W(YGR039W_d)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 Gene:YJL045W(YJL045W)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Minor succinate dehydrogenase isozyme; homologous to Sdh1p, the major isozyme reponsible for the oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner Gene:YKL096C-B(YKL096C-B_p)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPR063C(YPR063C_p)|FD-Score:-3.11|P-value:9.41E-4||SGD DESC:ER-localized protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL031W2.970.001510.17DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YGL150C2.900.001850.17INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YHR019C2.800.002530.17DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YNL113W2.740.003110.17RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YPR034W2.730.003130.17ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YGR145W2.640.004130.17ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YDL103C2.630.004280.17QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YGL137W2.460.007000.06SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YGL245W2.400.008220.05GUS1Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
YDL008W2.350.009450.02APC11Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
YBR029C2.330.009881.28E-4CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
YDR311W2.330.009880.00TFB1Subunit of TFIIH and nucleotide excision repair factor 3 complexes, required for nucleotide excision repair, target for transcriptional activators
YKL152C2.330.009970.02GPM1Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
YGR082W2.310.010600.00TOM20Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
YML127W2.300.010600.01RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C7.611.39E-14TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YER090W5.803.30E-9TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YNL147W4.846.39E-7LSM7Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
YDR354W4.592.27E-6TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YOR135C_d4.444.48E-6IRC14_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
YBR027C_d4.376.22E-6YBR027C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKR019C4.356.91E-6IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YGL118C_d3.993.34E-5YGL118C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR285W_p3.855.97E-5YBR285W_pPutative protein of unknown function; YBR285W is not an essential gene
YGL242C_p3.701.06E-4YGL242C_pPutative protein of unknown function; deletion mutant is viable
YDR346C3.691.14E-4SVF1Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance
YGR026W_p3.661.27E-4YGR026W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
YKR001C3.641.35E-4VPS1Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
YLR237W3.601.60E-4THI7Plasma membrane transporter responsible for the uptake of thiamine, member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
YJR117W3.581.74E-4STE24Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans

GO enrichment analysis for SGTC_1548
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3989.40E-224SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.240741
0.3581.66E-177SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0392157
0.3295.79E-149SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0615385
0.3141.53E-135SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.314815
0.3146.40E-135SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0645161
0.3131.88E-134SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.078125
0.2996.61E-122SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.0714286
0.2931.82E-117SGTC_18815652484 16.0 μMMiscellaneous22551290.0958904TRP & mitochondrial translation
0.2869.88E-112SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0243902
0.2843.05E-110SGTC_1104tacrolimus 29.8 μMNIH Clinical Collection235817960.079646
0.2816.84E-108SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.150943
0.2811.08E-107SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.0806452
0.2792.89E-106SGTC_1114scopoletin 1.7 μMTimTec (Natural product library)52804600.355556
0.2783.74E-105SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.107692
0.2785.71E-105SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0491803

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_15447-methoxy-4-methylcoumarin105 μM0.564103390807TimTec (Pure natural product library)190.19532.32903
SGTC_1518st03856790.8 μM0.55263277966TimTec (Pure natural product library)220.221282.31304
SGTC_2556citropten84.59 μM0.5384622775Microsource (Natural product library)206.19471.86604
SGTC_2550chrysin dimethyl ether71.87 μM0.51111188881Microsource (Natural product library)282.290663.10304
SGTC_1929genistein trimethyl ether50.5 μM0.425532136420TimTec (Natural product derivative library)312.316642.81705
SGTC_1904st06022578 μM0.42222266595TimTec (Natural product derivative library)204.221882.67803
SGTC_2492derrusnin100 μM0.4150941715307Microsource (Natural product library)356.326142.80107
SGTC_1867st05737264 μM0.413043688837TimTec (Natural product derivative library)312.316643.08705
SGTC_15666,7-dimethoxy-4-ethylcoumarin85.4 μM0.409091688761TimTec (Pure natural product library)234.247862.76904
SGTC_1912st06020990.8 μM0.404255688787TimTec (Natural product derivative library)220.221281.9204