4'-methoxyflavonol

3-hydroxy-2-(4-methoxyphenyl)chromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1549
Screen concentration 74.6 μM
Source TimTec (Pure natural product library)
PubChem CID 97141
SMILES COC1=CC=C(C=C1)C2=C(C(=O)C3=CC=CC=C3O2)O
Standardized SMILES COc1ccc(cc1)C2=C(O)C(=O)c3ccccc3O2
Molecular weight 268.2641
ALogP 2.58
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 97141
Download HIP data (tab-delimited text)  (excel)
Gene:COF1(YLL050C)|FD-Score:-3.26|P-value:5.48E-4|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:MCM6(YGL201C)|FD-Score:3.13|P-value:8.84E-4|Clearance:0.08||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:RRN9(YMR270C)|FD-Score:-4.13|P-value:1.78E-5|Clearance:0||SGD DESC:Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I Gene:SLD5(YDR489W)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.11||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:TEL2(YGR099W)|FD-Score:4.57|P-value:2.43E-6|Clearance:1.34||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:COF1(YLL050C)|FD-Score:-3.26|P-value:5.48E-4|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:MCM6(YGL201C)|FD-Score:3.13|P-value:8.84E-4|Clearance:0.08||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p Gene:RRN9(YMR270C)|FD-Score:-4.13|P-value:1.78E-5|Clearance:0||SGD DESC:Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I Gene:SLD5(YDR489W)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.11||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:TEL2(YGR099W)|FD-Score:4.57|P-value:2.43E-6|Clearance:1.34||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 97141
Download HOP data (tab-delimited text)  (excel)
Gene:ACF4(YJR083C)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ADY2(YCR010C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:ALD6(YPL061W)|FD-Score:-3.74|P-value:9.03E-5||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ATG27(YJL178C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:AZF1(YOR113W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:CBR1(YIL043C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CUE5(YOR042W)|FD-Score:-3.16|P-value:7.80E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ERG4(YGL012W)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GEP7(YGL057C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:LAG2(YOL025W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LOH1(YJL038C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MDL1(YLR188W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MEP1(YGR121C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MRPL40(YPL173W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB2(YGR014W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MUS81(YDR386W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NPT1(YOR209C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:PGD1(YGL025C)|FD-Score:4.4|P-value:5.36E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO91(YNR013C)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PPZ1(YML016C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RML2(YEL050C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL13B(YMR142C)|FD-Score:5.46|P-value:2.35E-8||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:SAY1(YGR263C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SHO1(YER118C)|FD-Score:-3.83|P-value:6.49E-5||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SVF1(YDR346C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TRP3(YKL211C)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YDR442W(YDR442W_d)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER152C(YER152C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YFL013W-A(YFL013W-A_d)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YGR039W(YGR039W_d)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 Gene:YKR047W(YKR047W_d)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YMR122C(YMR122C_d)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ACF4(YJR083C)|FD-Score:-3.52|P-value:2.13E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ADY2(YCR010C)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:ALD6(YPL061W)|FD-Score:-3.74|P-value:9.03E-5||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ATG27(YJL178C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:AZF1(YOR113W)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Zinc-finger transcription factor; in the presence of glucose, activates transcription of genes involved in growth and carbon metabolism; in nonfermentable carbon sources, activates transcription of genes involved in maintenance of cell wall integrity Gene:CBR1(YIL043C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CUE5(YOR042W)|FD-Score:-3.16|P-value:7.80E-4||SGD DESC:Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ERG4(YGL012W)|FD-Score:4.63|P-value:1.86E-6||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GEP7(YGL057C)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:LAG2(YOL025W)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Protein that negatively regulates the SCF E3-ubiquitin ligase by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1 Gene:LOH1(YJL038C)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MDL1(YLR188W)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MEP1(YGR121C)|FD-Score:-4.16|P-value:1.62E-5||SGD DESC:Ammonium permease; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MRPL40(YPL173W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB2(YGR014W)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MUS81(YDR386W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NPT1(YOR209C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:PGD1(YGL025C)|FD-Score:4.4|P-value:5.36E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHO91(YNR013C)|FD-Score:3.15|P-value:8.10E-4||SGD DESC:Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth Gene:PPZ1(YML016C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RML2(YEL050C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL13B(YMR142C)|FD-Score:5.46|P-value:2.35E-8||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:SAY1(YGR263C)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum Gene:SHO1(YER118C)|FD-Score:-3.83|P-value:6.49E-5||SGD DESC:Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p Gene:SVF1(YDR346C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein with a potential role in cell survival pathways, required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance Gene:TRP3(YKL211C)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:4.94|P-value:3.87E-7||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:YDR442W(YDR442W_d)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER152C(YER152C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YFL013W-A(YFL013W-A_d)|FD-Score:-3.36|P-value:3.95E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YGR039W(YGR039W_d)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722 Gene:YKR047W(YKR047W_d)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YMR122C(YMR122C_d)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR099W4.572.43E-61.34TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YDR489W3.236.15E-40.10SLD5Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YGL201C3.138.84E-40.08MCM6Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
YOR341W3.040.001170.03RPA190RNA polymerase I largest subunit A190
YDL028C3.020.001270.23MPS1Dual-specificity kinase; required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p
YLR230W_d2.790.002640.03YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
YGR145W2.760.002870.06ENP2Essential nucleolar protein, required for biogenesis of the small ribosomal subunit; contains WD repeats, interacts with Mpp10p and Bfr2p, and has homology to Spb1p
YDR324C2.710.003400.17UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YJL104W2.540.005510.05PAM16Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain
YDL087C2.490.006380.01LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YPR082C2.480.006640.03DIB117-kDa component of the U4/U6aU5 tri-snRNP, plays an essential role in pre-mRNA splicing, orthologue of hDIM1, the human U5-specific 15-kDa protein
YJL195C_d2.450.007167.05E-4YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YGR048W2.450.007170.00UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YGL111W2.450.007200.09NSA1Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
YBR029C2.360.009120.01CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR142C5.462.35E-8RPL13BRibosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
YBR058C-A4.943.87E-7TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGL012W4.631.86E-6ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YGL057C4.592.26E-6GEP7Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGL025C4.405.36E-6PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YLR188W4.191.37E-5MDL1Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress
YGR263C4.151.68E-5SAY1Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum
YMR122C_d3.846.19E-5YMR122C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKL211C3.768.47E-5TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YIL043C3.671.23E-4CBR1Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia
YDR442W_d3.591.66E-4YDR442W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YEL050C3.482.50E-4RML2Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor
YPL173W3.482.54E-4MRPL40Mitochondrial ribosomal protein of the large subunit
YGR039W_d3.462.70E-4YGR039W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722
YML016C3.452.84E-4PPZ1Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance

GO enrichment analysis for SGTC_1549
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2195.50E-65SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0625TSC3-RPN4
0.2016.61E-55SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.03125TSC3-RPN4
0.1771.02E-42SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0634921TSC3-RPN4
0.1771.07E-42SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0967742TSC3-RPN4
0.1731.02E-40SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.106667TSC3-RPN4
0.1681.05E-38SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.169231TSC3-RPN4
0.1652.20E-37SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.174603TSC3-RPN4
0.1583.38E-34SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.175439
0.1561.55E-33SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.2TSC3-RPN4
0.1561.98E-33SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0757576TSC3-RPN4
0.1556.06E-33SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.046875TSC3-RPN4
0.1541.40E-32SGTC_10134112-3890 241.0 μMChemDiv (Drug-like library)7051710.171429TSC3-RPN4
0.1527.74E-32SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0555556
0.1504.79E-31SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.138462TSC3-RPN4
0.1497.70E-31SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.0985916TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_11103-hydroxy-3'-methoxyflavone1.24 μM0.738095676296TimTec (Natural product library)268.264082.58214iron homeostasis
SGTC_1864st05764530.4 μM0.725688666TimTec (Natural product derivative library)282.290662.80804amide catabolism
SGTC_1112tnp000691.17 μM0.619048145826TimTec (Natural product library)270.23692.11435
SGTC_1109tnp00067928.26 nM0.581395676295TimTec (Natural product library)254.23752.35624
SGTC_1830st05598250.3 μM0.575455313TimTec (Natural product derivative library)254.23752.35624
SGTC_26222-methoxyxanthone59.94 μM0.54761971034Microsource (Natural product library)226.22742.94803
SGTC_14204'-methoxyflavone42 μM0.54545577793ChemDiv (Drug-like library)252.264683.1203
SGTC_26344'-methoxyflavone100 μM0.54545577793Microsource (Natural product library)252.264683.1203
SGTC_1868st05764442.7 μM0.531915688665TimTec (Natural product derivative library)282.290662.80804
SGTC_18693',7'-dimethoxyflavone40.3 μM0.510638688664TimTec (Natural product derivative library)282.290662.80804
SGTC_25623',7'-dimethoxyflavone64.92 μM0.510638688664Microsource (Natural product library)282.290662.80804
SGTC_15823',3',6'-trihydroxyflavone74 μM0.446809688803TimTec (Pure natural product library)270.23692.11435