gossypolone

7-(8-formyl-6,7-dihydroxy-3-methyl-1,4-dioxo-5-propan-2-ylnaphthalen-2-yl)-2,3-dihydroxy-6-methyl-5,8-dioxo-4-propan-2-ylnaphthalene-1-carbaldehyde

Gossypolone is a phenolic aldehyde that permeates cells and acts as an inhibitor for several dehydrogenase enzymes.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1550
Screen concentration 36.6 μM
Source TimTec (Pure natural product library)
PubChem CID 5478622
SMILES CC1=C(C(=O)C2=C(C1=O)C(=C(C(=C2C=O)O)O)C(C)C)C3=C(C(=O)C4=C(C3=O)C(=C(C(=C4C(C)C)O)O)C=O)C
Standardized SMILES CC(C)c1c(O)c(O)c(C=O)c2C(=O)C(=C(C)C(=O)c12)C3=C(C)C(=O)c4c(C(C)C)c(O)c(O)c(C=O)c4C3=O
Molecular weight 546.5214
ALogP 4.96
H-bond donor count 4
H-bond acceptor count 10
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.96
% growth inhibition (Hom. pool) 3.05


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5478622
Download HIP data (tab-delimited text)  (excel)
Gene:ARP9(YMR033W)|FD-Score:-5.36|P-value:4.19E-8|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BET5(YML077W)|FD-Score:-4.32|P-value:7.91E-6|Clearance:0||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:DIS3(YOL021C)|FD-Score:3.83|P-value:6.46E-5|Clearance:0.14||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:GWT1(YJL091C)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.06||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:MNP1(YGL068W)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.02||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NEO1(YIL048W)|FD-Score:4.28|P-value:9.46E-6|Clearance:0.45||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:POP5(YAL033W)|FD-Score:4.37|P-value:6.34E-6|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPC82(YPR190C)|FD-Score:-4.96|P-value:3.51E-7|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPL32(YBL092W)|FD-Score:3.45|P-value:2.80E-4|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPT4(YOR259C)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.18||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SEC10(YLR166C)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.25||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SEC31(YDL195W)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.03||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SRP54(YPR088C)|FD-Score:5.4|P-value:3.26E-8|Clearance:0.87||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TIM10(YHR005C-A)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.13||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YKL036C(YKL036C_d)|FD-Score:4.53|P-value:2.95E-6|Clearance:0.16||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:ARP9(YMR033W)|FD-Score:-5.36|P-value:4.19E-8|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BET5(YML077W)|FD-Score:-4.32|P-value:7.91E-6|Clearance:0||SGD DESC:Component of the TRAPP (transport protein particle) complex, which plays an essential role in the vesicular transport from endoplasmic reticulum to Golgi Gene:DIS3(YOL021C)|FD-Score:3.83|P-value:6.46E-5|Clearance:0.14||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:GWT1(YJL091C)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.06||SGD DESC:Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors Gene:MNP1(YGL068W)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.02||SGD DESC:Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth Gene:NEO1(YIL048W)|FD-Score:4.28|P-value:9.46E-6|Clearance:0.45||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:POP5(YAL033W)|FD-Score:4.37|P-value:6.34E-6|Clearance:0.09||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPC82(YPR190C)|FD-Score:-4.96|P-value:3.51E-7|Clearance:0||SGD DESC:RNA polymerase III subunit C82 Gene:RPL32(YBL092W)|FD-Score:3.45|P-value:2.80E-4|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPT4(YOR259C)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.18||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SEC10(YLR166C)|FD-Score:3.18|P-value:7.46E-4|Clearance:0.25||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SEC31(YDL195W)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.03||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SRP54(YPR088C)|FD-Score:5.4|P-value:3.26E-8|Clearance:0.87||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TIM10(YHR005C-A)|FD-Score:3.35|P-value:4.06E-4|Clearance:0.13||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YKL036C(YKL036C_d)|FD-Score:4.53|P-value:2.95E-6|Clearance:0.16||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5478622
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:8.51|P-value:8.88E-18||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APL6(YGR261C)|FD-Score:5.69|P-value:6.30E-9||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ATG11(YPR049C)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BNA1(YJR025C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CAP2(YIL034C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCC2(YDR270W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:COA2(YPL189C-A)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:EIS1(YMR031C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ERG3(YLR056W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FMP52(YER004W)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GAT4(YIR013C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GCY1(YOR120W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GEA2(YEL022W)|FD-Score:5.25|P-value:7.72E-8||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HAT2(YEL056W)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing Gene:HSP82(YPL240C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HXT2(YMR011W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IDH2(YOR136W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IRC6(YFR043C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LCB3(YJL134W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication Gene:LDB16(YCL005W)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:MRC1(YCL061C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL10(YNL284C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:OPI8(YKR035C_d)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OPI9(YLR338W_d)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:POF1(YCL047C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PRY2(YKR013W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RNH201(YNL072W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:ROM2(YLR371W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RRT5(YFR032C_p)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:SBE22(YHR103W)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SDH4(YDR178W)|FD-Score:-3.94|P-value:4.03E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SKM1(YOL113W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SPT21(YMR179W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT3(YDR392W)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:THI4(YGR144W)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TPN1(YGL186C)|FD-Score:-3.79|P-value:7.38E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSC3(YBR058C-A)|FD-Score:4.42|P-value:4.88E-6||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP1(YDL122W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:URA1(YKL216W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VMA13(YPR036W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS21(YOR089C)|FD-Score:4.27|P-value:9.68E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS33(YLR396C)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:VPS35(YJL154C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:XBP1(YIL101C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAR028W(YAR028W_p)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBL083C(YBL083C_d)|FD-Score:-3.79|P-value:7.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCL046W(YCL046W_d)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDR455C(YDR455C_d)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGP1(YNL160W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR237C(YGR237C_p)|FD-Score:-3.92|P-value:4.35E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR290W(YGR290W_d)|FD-Score:4.33|P-value:7.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YKL118W(YKL118W_d)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YNL010W(YNL010W_p)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOL073C(YOL073C_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YOL106W(YOL106W_d)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL261C(YPL261C_d)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPR123C(YPR123C_d)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:AFT1(YGL071W)|FD-Score:8.51|P-value:8.88E-18||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APL6(YGR261C)|FD-Score:5.69|P-value:6.30E-9||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ATG11(YPR049C)|FD-Score:3.91|P-value:4.63E-5||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:BNA1(YJR025C)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:3-hydroxyanthranilic acid dioxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:CAP2(YIL034C)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCC2(YDR270W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:COA2(YPL189C-A)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p Gene:EIS1(YMR031C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ERG3(YLR056W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:FMP52(YER004W)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GAT4(YIR013C)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Protein containing GATA family zinc finger motifs Gene:GCY1(YOR120W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GEA2(YEL022W)|FD-Score:5.25|P-value:7.72E-8||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HAT2(YEL056W)|FD-Score:-3.1|P-value:9.66E-4||SGD DESC:Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing Gene:HSP82(YPL240C)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HXT2(YMR011W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IDH2(YOR136W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IRC6(YFR043C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LCB3(YJL134W)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication Gene:LDB16(YCL005W)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:MRC1(YCL061C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:MRPL10(YNL284C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:OPI8(YKR035C_d)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:OPI9(YLR338W_d)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:POF1(YCL047C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PRY2(YKR013W)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RNH201(YNL072W)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:ROM2(YLR371W)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RRT5(YFR032C_p)|FD-Score:3.13|P-value:8.69E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation Gene:SBE22(YHR103W)|FD-Score:-3.54|P-value:2.02E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SDH4(YDR178W)|FD-Score:-3.94|P-value:4.03E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SKM1(YOL113W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SPT21(YMR179W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPT3(YDR392W)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:THI4(YGR144W)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TPN1(YGL186C)|FD-Score:-3.79|P-value:7.38E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSC3(YBR058C-A)|FD-Score:4.42|P-value:4.88E-6||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP1(YDL122W)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:URA1(YKL216W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid Gene:VMA13(YPR036W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS21(YOR089C)|FD-Score:4.27|P-value:9.68E-6||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:VPS3(YDR495C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS33(YLR396C)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:VPS35(YJL154C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:XBP1(YIL101C)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAR028W(YAR028W_p)|FD-Score:3.87|P-value:5.34E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBL083C(YBL083C_d)|FD-Score:-3.79|P-value:7.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCL046W(YCL046W_d)|FD-Score:4.56|P-value:2.57E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C Gene:YDR455C(YDR455C_d)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGP1(YNL160W)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; has similarity to Sps100p Gene:YGR237C(YGR237C_p)|FD-Score:-3.92|P-value:4.35E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YGR290W(YGR290W_d)|FD-Score:4.33|P-value:7.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YKL118W(YKL118W_d)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YNL010W(YNL010W_p)|FD-Score:3.09|P-value:9.90E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YOL073C(YOL073C_p)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YOL106W(YOL106W_d)|FD-Score:4.18|P-value:1.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL261C(YPL261C_d)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YPL260W Gene:YPR123C(YPR123C_d)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR088C5.403.26E-80.87SRP54Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
YKL036C_d4.532.95E-60.16YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
YAL033W4.376.34E-60.09POP5Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YIL048W4.289.46E-60.45NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YOL021C3.836.46E-50.14DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YOR259C3.681.15E-40.18RPT4One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
YJL091C3.512.27E-40.06GWT1Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
YBL092W3.452.80E-40.10RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YHR005C-A3.354.06E-40.13TIM10Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YGL068W3.226.41E-40.02MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YDL195W3.206.81E-40.03SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YLR166C3.187.46E-40.25SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YDL205C2.930.001690.01HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YDR302W2.920.001740.09GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YFL034C-B2.830.002300.06MOB2Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL071W8.518.88E-18AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YGR261C5.696.30E-9APL6Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
YEL022W5.257.72E-8GEA2Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
YPR123C_d4.721.16E-6YPR123C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
YCL046W_d4.562.57E-6YCL046W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
YLR396C4.483.74E-6VPS33ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole
YBR058C-A4.424.88E-6TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGR290W_d4.337.31E-6YGR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
YOR089C4.279.68E-6VPS21Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
YNL072W4.191.41E-5RNH201Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
YOL106W_d4.181.43E-5YOL106W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR031C4.111.98E-5EIS1Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YPR049C3.914.63E-5ATG11Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS)
YER004W3.885.22E-5FMP52Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation
YAR028W_p3.875.34E-5YAR028W_pPutative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS

GO enrichment analysis for SGTC_1550
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1541.61E-32SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.0263158NEO1
0.1431.68E-28SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.0294118NEO1
0.1344.89E-25SGTC_9001112-0045 141.0 μMChemDiv (Drug-like library)562110.0483871NEO1
0.1285.61E-23SGTC_1665st014639 11.4 μMTimTec (Natural product derivative library)9811330.0705882NEO1
0.1248.15E-22SGTC_270tamoxifen 5.5 μMMiscellaneous53760.0307692NEO1
0.1224.11E-21SGTC_2064546-0025 72.0 μMChemDiv (Drug-like library)66207450.0547945NEO1
0.1201.50E-20SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0862069TSC3-RPN4
0.1202.79E-20SGTC_1721112-0046 2.6 μMChemDiv (Drug-like library)28021350.0454545NEO1
0.1186.77E-20SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.109091TSC3-RPN4
0.1181.15E-19SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0735294TSC3-RPN4
0.1171.36E-19SGTC_1554st069299 38.7 μMTimTec (Pure natural product library)242079990.232143
0.1155.73E-19SGTC_1530rhein 70.4 μMTimTec (Pure natural product library)101680.122807
0.1141.36E-18SGTC_232clozapine 119.0 μMMiscellaneous28180.028169NEO1
0.1141.38E-18SGTC_1660st012960 28.1 μMTimTec (Natural product derivative library)242072660.0571429iron homeostasis
0.1128.20E-18SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.016129TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1553gossypol38.6 μM0.4444443503TimTec (Pure natural product library)518.55446.59268
SGTC_2516gossypol71.55 μM0.4444443503TimTec (Pure natural product library)518.55446.59268
SGTC_1554st06929938.7 μM0.23214324207999TimTec (Pure natural product library)516.584885.77368
SGTC_2506ethyl hematommate54.16 μM0.2222223940691Microsource (Natural product library)224.209981.79525RPP1 & pyrimidine depletion
SGTC_8570438-03061.35 μM0.2142864686136ChemDiv (Drug-like library)262.1323362.93607copper-dependent oxidative stress
SGTC_1558st06930045.6 μM0.21153824208000TimTec (Pure natural product library)438.51284.44746
SGTC_2608thymoquinone42.66 μM0.18181810281Microsource (Natural product library)164.201082.28802
SGTC_1556plumbagin1.48 μM0.1810205TimTec (Pure natural product library)188.179421.96213superoxide
SGTC_2575plumbagin510 nM0.1810205TimTec (Pure natural product library)188.179421.96213superoxide
SGTC_3561634-001232.5 μM0.176471735457ChemDiv (Drug-like library)251.076083.00412
SGTC_1180083-0097135.34 μM0.1733335207356ChemDiv (Drug-like library)623.373285.56515translation
SGTC_3640083-009767.7 μM0.1733335207356ChemDiv (Drug-like library)623.373285.56515
SGTC_263alizarin107.93 μM0.1666676293Miscellaneous240.210922.32424iron homeostasis