6-bromo-3'-methylflavone

6-bromo-2-(3-methylphenyl)chromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1551
Screen concentration 63.5 μM
Source TimTec (Pure natural product library)
PubChem CID 688751
SMILES CC1=CC=CC(=C1)C2=CC(=O)C3=C(O2)C=CC(=C3)Br
Standardized SMILES Cc1cccc(c1)C2=CC(=O)c3cc(Br)ccc3O2
Molecular weight 315.1613
ALogP 4.37
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.36
% growth inhibition (Hom. pool) 5.35


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 688751
Download HIP data (tab-delimited text)  (excel)
Gene:CDC34(YDR054C)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.09||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:FCP1(YMR277W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FRQ1(YDR373W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.07||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:NMD3(YHR170W)|FD-Score:3.88|P-value:5.20E-5|Clearance:0.4||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC4(YPR144C)|FD-Score:-3.18|P-value:7.34E-4|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:PGA1(YNL158W)|FD-Score:3.25|P-value:5.75E-4|Clearance:0.03||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:RPA43(YOR340C)|FD-Score:-3.42|P-value:3.15E-4|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:SRP54(YPR088C)|FD-Score:3.13|P-value:8.72E-4|Clearance:0.13||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:WBP1(YEL002C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YBL077W(YBL077W_d)|FD-Score:-3.16|P-value:7.87E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YKL036C(YKL036C_d)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.15||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W Gene:CDC34(YDR054C)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.09||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:FCP1(YMR277W)|FD-Score:3.48|P-value:2.51E-4|Clearance:0||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:FRQ1(YDR373W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.07||SGD DESC:N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily Gene:NMD3(YHR170W)|FD-Score:3.88|P-value:5.20E-5|Clearance:0.4||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOC4(YPR144C)|FD-Score:-3.18|P-value:7.34E-4|Clearance:0||SGD DESC:Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits Gene:PGA1(YNL158W)|FD-Score:3.25|P-value:5.75E-4|Clearance:0.03||SGD DESC:Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes Gene:RPA43(YOR340C)|FD-Score:-3.42|P-value:3.15E-4|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:SRP54(YPR088C)|FD-Score:3.13|P-value:8.72E-4|Clearance:0.13||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:WBP1(YEL002C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum Gene:YBL077W(YBL077W_d)|FD-Score:-3.16|P-value:7.87E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:YKL036C(YKL036C_d)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.15||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 688751
Download HOP data (tab-delimited text)  (excel)
Gene:AFG1(YEL052W)|FD-Score:-3.99|P-value:3.37E-5||SGD DESC:Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain Gene:ATP3(YBR039W)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVT4(YNL101W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BUD27(YFL023W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CAR1(YPL111W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CBP4(YGR174C)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CCC2(YDR270W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CDH1(YGL003C)|FD-Score:4.8|P-value:8.10E-7||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CIN4(YMR138W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CTI6(YPL181W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DAL3(YIR032C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL4(YIR028W)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:ECI1(YLR284C)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:EXG2(YDR261C)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FHN1(YGR131W)|FD-Score:4.93|P-value:4.16E-7||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FMP37(YGL080W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:FUM1(YPL262W)|FD-Score:3.75|P-value:8.72E-5||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GAL11(YOL051W)|FD-Score:4.77|P-value:9.29E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GEA2(YEL022W)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GPB1(YOR371C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:HEL1(YKR017C_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HPR1(YDR138W)|FD-Score:-4.46|P-value:4.08E-6||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IRC19(YLL033W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:LIP2(YLR239C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MET6(YER091C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs Gene:MRC1(YCL061C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:NCR1(YPL006W)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NUM1(YDR150W)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OCA4(YCR095C_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PDR1(YGL013C)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR8(YLR266C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PMP1(YCR024C-A)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PTH1(YHR189W)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RAD17(YOR368W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RAD27(YKL113C)|FD-Score:-4.65|P-value:1.70E-6||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:REG1(YDR028C)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM20(YOR275C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RNH201(YNL072W)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL17B(YJL177W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL27B(YDR471W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTS3(YGR161C_p)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SAP30(YMR263W)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SHE1(YBL031W)|FD-Score:-4.39|P-value:5.70E-6||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SHE4(YOR035C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SPO11(YHL022C)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:STE50(YCL032W)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SWI6(YLR182W)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TRP3(YKL211C)|FD-Score:5.55|P-value:1.47E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UIP4(YPL186C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VMA1(YDL185W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPH1(YOR270C)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS62(YGR141W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:YAL018C(YAL018C_p)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Putative protein of unknown function Gene:YBR182C-A(YBR182C-A_p)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCL002C(YCL002C_p)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YGR250C(YGR250C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YJL147C(YJL147C_p)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YKR070W(YKR070W_p)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function Gene:YLR455W(YLR455W_p)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YMR193C-A(YMR193C-A_d)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL122C(YNL122C_p)|FD-Score:4|P-value:3.14E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNL276C(YNL276C_d)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YNL319W(YNL319W_d)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR041C(YOR041C_d)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR114W(YOR114W_p)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:AFG1(YEL052W)|FD-Score:-3.99|P-value:3.37E-5||SGD DESC:Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain Gene:ATP3(YBR039W)|FD-Score:3.78|P-value:7.82E-5||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AVT4(YNL101W)|FD-Score:3.26|P-value:5.54E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BUD27(YFL023W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CAR1(YPL111W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Arginase, responsible for arginine degradation, expression responds to both induction by arginine and nitrogen catabolite repression; disruption enhances freeze tolerance Gene:CBP4(YGR174C)|FD-Score:3.9|P-value:4.76E-5||SGD DESC:Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex Gene:CCC2(YDR270W)|FD-Score:3.2|P-value:6.86E-4||SGD DESC:Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism Gene:CDH1(YGL003C)|FD-Score:4.8|P-value:8.10E-7||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CIN4(YMR138W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CTI6(YPL181W)|FD-Score:3.27|P-value:5.31E-4||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DAL3(YIR032C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression Gene:DAL4(YIR028W)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:ECI1(YLR284C)|FD-Score:3.8|P-value:7.16E-5||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:EXG2(YDR261C)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FHN1(YGR131W)|FD-Score:4.93|P-value:4.16E-7||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FMP37(YGL080W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog is associated with lactic acidosis and hyperpyruvatemia Gene:FUM1(YPL262W)|FD-Score:3.75|P-value:8.72E-5||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GAL11(YOL051W)|FD-Score:4.77|P-value:9.29E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GEA2(YEL022W)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GPB1(YOR371C)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication Gene:HEL1(YKR017C_p)|FD-Score:3.28|P-value:5.15E-4||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HPR1(YDR138W)|FD-Score:-4.46|P-value:4.08E-6||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IRC19(YLL033W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci Gene:LIP2(YLR239C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MET6(YER091C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs Gene:MRC1(YCL061C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:NCR1(YPL006W)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NUM1(YDR150W)|FD-Score:3.09|P-value:9.84E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OCA4(YCR095C_p)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:PDR1(YGL013C)|FD-Score:4.12|P-value:1.87E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR8(YLR266C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PMP1(YCR024C-A)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PTH1(YHR189W)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RAD17(YOR368W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RAD27(YKL113C)|FD-Score:-4.65|P-value:1.70E-6||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:REG1(YDR028C)|FD-Score:3.8|P-value:7.33E-5||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM20(YOR275C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation Gene:RNH201(YNL072W)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL17B(YJL177W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL27B(YDR471W)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTS3(YGR161C_p)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SAP30(YMR263W)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SHE1(YBL031W)|FD-Score:-4.39|P-value:5.70E-6||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SHE4(YOR035C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SPO11(YHL022C)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:STE50(YCL032W)|FD-Score:3.9|P-value:4.79E-5||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:SWI6(YLR182W)|FD-Score:3.87|P-value:5.52E-5||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TRP3(YKL211C)|FD-Score:5.55|P-value:1.47E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:UIP4(YPL186C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VMA1(YDL185W)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VPH1(YOR270C)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS62(YGR141W)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Vacuolar protein sorting (VPS) protein required for cytoplasm to vacuole targeting of proteins Gene:YAL018C(YAL018C_p)|FD-Score:3.26|P-value:5.64E-4||SGD DESC:Putative protein of unknown function Gene:YBR182C-A(YBR182C-A_p)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCL002C(YCL002C_p)|FD-Score:4.15|P-value:1.66E-5||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.97|P-value:3.63E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YGR250C(YGR250C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YJL147C(YJL147C_p)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YKR070W(YKR070W_p)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YLR264C-A(YLR264C-A_p)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:Putative protein of unknown function Gene:YLR455W(YLR455W_p)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YMR193C-A(YMR193C-A_d)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL122C(YNL122C_p)|FD-Score:4|P-value:3.14E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene Gene:YNL276C(YNL276C_d)|FD-Score:-3.31|P-value:4.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YNL319W(YNL319W_d)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR041C(YOR041C_d)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:YOR114W(YOR114W_p)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Putative protein of unknown function; null mutant is viable

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR170W3.885.20E-50.40NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YMR277W3.482.51E-40.00FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YDR373W3.482.54E-40.07FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YKL036C_d3.403.33E-40.15YKL036C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the essential gene UGP1 gene and uncharacterized ORF YKL037W
YNL158W3.255.75E-40.03PGA1Essential component of GPI-mannosyltransferase II, responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interations with secretory pathway genes
YDR054C3.236.29E-40.09CDC34Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
YPR088C3.138.72E-40.13SRP54Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
YOR206W3.000.001340.06NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YGL008C2.940.001630.07PMA1Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
YLR007W2.870.002030.07NSE1Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YPL142C_d2.800.002540.17YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YGL099W2.630.004240.02LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YLR076C_d2.610.004480.06YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YAL025C2.550.005350.04MAK16Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus
YBR237W2.520.005920.01PRP5RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C5.551.47E-8TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGR131W4.934.16E-7FHN1Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain
YGL003C4.808.10E-7CDH1Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p
YOL051W4.779.29E-7GAL11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
YNL072W4.691.34E-6RNH201Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
YIR032C4.612.02E-6DAL3Ureidoglycolate hydrolase, converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression sensitive to nitrogen catabolite repression
YCL002C_p4.151.66E-5YCL002C_pPutative protein of unknown function; YCL002C is not an essential gene
YGL013C4.121.87E-5PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YGR161C_p4.072.37E-5RTS3_pPutative component of the protein phosphatase type 2A complex
YOR114W_p4.052.61E-5YOR114W_pPutative protein of unknown function; null mutant is viable
YNL122C_p4.003.14E-5YNL122C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene
YCR087C-A_p3.973.63E-5YCR087C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
YCR024C-A3.963.76E-5PMP1Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication
YDR261C3.924.45E-5EXG2Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
YGR174C3.904.76E-5CBP4Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex

GO enrichment analysis for SGTC_1551
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1132.81E-18SGTC_2752diclazuril 33.1 μMMiscellaneous4563890.08
0.1063.70E-16SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0804598PDR1
0.1015.72E-15SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.0909091
0.0979.70E-14SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0769231TSC3-RPN4
0.0944.36E-13SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0350877
0.0911.95E-12SGTC_22967971656 126.5 μMChembridge (Fragment library)6665700.0958904
0.0903.33E-12SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.109375
0.0903.52E-12SGTC_1909st060837 51.5 μMTimTec (Natural product derivative library)6789370.25
0.0897.17E-12SGTC_11941488-0194 225.0 μMChemDiv (Drug-like library)13914520.102941
0.0872.29E-11SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.0952381PDR1
0.0864.06E-11SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.4
0.0855.06E-11SGTC_2416tofa 880.0 nMMiscellaneous1151750.09375sphingolipid biosynthesis & PDR1
0.0855.74E-11SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.0833333PDR1
0.0855.88E-11SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.182927PDR1
0.0856.40E-11SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0963855PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_15526-methylflavone10.6 μM0.642857689013TimTec (Pure natural product library)236.265283.62202superoxide
SGTC_18943',6'-dihydroxyflavone78.7 μM0.555556688662TimTec (Natural product derivative library)254.23752.65224
SGTC_1857st05600779.3 μM0.489796619834TimTec (Natural product derivative library)252.264683.1203
SGTC_1887st05764870.8 μM0.46688672TimTec (Natural product derivative library)282.290663.10304
SGTC_15427-hydroxy-3'-methoxyflavone49.7 μM0.4259265393153TimTec (Pure natural product library)268.264082.87814
SGTC_1111tnp00062510.7 nM0.423077676306TimTec (Natural product library)268.264082.87814
SGTC_1860st05622874.6 μM0.423077688679TimTec (Natural product derivative library)268.264082.87814
SGTC_1732st03430851.82 μM0.416667830119TimTec (Natural product derivative library)277.531083.63602
SGTC_1838st05536984.5 μM0.416667676036TimTec (Natural product derivative library)236.265283.62202
SGTC_24977-hydroxyflavone100 μM0.4081635281894Microsource (Natural product library)238.23812.89413