st069300

6-(2,4-dihydroxy-6-methyl-3-oxo-1-propan-2-yl-1,2-dihydroinden-5-yl)-2,7-dihydroxy-5-methyl-3-propan-2-yl-2,3-dihydroinden-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1558
Screen concentration 45.6 μM
Source TimTec (Pure natural product library)
PubChem CID 24208000
SMILES CC1=C(C(=C2C(=C1)C(C(C2=O)O)C(C)C)O)C3=C(C=C4C(C(C(=O)C4=C3O)O)C(C)C)C
Standardized SMILES CC(C)C1C(O)C(=O)c2c(O)c(c(C)cc12)c3c(C)cc4C(C(C)C)C(O)C(=O)c4c3O
Molecular weight 438.5128
ALogP 4.45
H-bond donor count 4
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.92
% growth inhibition (Hom. pool) 2.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24208000
Download HIP data (tab-delimited text)  (excel)
Gene:ATP16(YDL004W)|FD-Score:-5.06|P-value:2.13E-7|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:ERO1(YML130C)|FD-Score:3.17|P-value:7.70E-4|Clearance:0.02||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:GPI15(YNL038W)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.26||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:KEI1(YDR367W)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.02||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MCM2(YBL023C)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.06||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MTR3(YGR158C)|FD-Score:3.24|P-value:6.08E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:PRE8(YML092C)|FD-Score:-3.23|P-value:6.20E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RFC5(YBR087W)|FD-Score:3.89|P-value:5.07E-5|Clearance:0.19||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPA43(YOR340C)|FD-Score:7.54|P-value:2.35E-14|Clearance:3.65||SGD DESC:RNA polymerase I subunit A43 Gene:RPT5(YOR117W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.2||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:YDR396W(YDR396W_d)|FD-Score:3.15|P-value:8.13E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex Gene:ATP16(YDL004W)|FD-Score:-5.06|P-value:2.13E-7|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:ERO1(YML130C)|FD-Score:3.17|P-value:7.70E-4|Clearance:0.02||SGD DESC:Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds Gene:GPI15(YNL038W)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.26||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein Gene:KEI1(YDR367W)|FD-Score:3.11|P-value:9.49E-4|Clearance:0.02||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MCM2(YBL023C)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.06||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MTR3(YGR158C)|FD-Score:3.24|P-value:6.08E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:PRE8(YML092C)|FD-Score:-3.23|P-value:6.20E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RFC5(YBR087W)|FD-Score:3.89|P-value:5.07E-5|Clearance:0.19||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPA43(YOR340C)|FD-Score:7.54|P-value:2.35E-14|Clearance:3.65||SGD DESC:RNA polymerase I subunit A43 Gene:RPT5(YOR117W)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.2||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:YDR396W(YDR396W_d)|FD-Score:3.15|P-value:8.13E-4|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24208000
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AMA1(YGR225W)|FD-Score:-3.74|P-value:9.24E-5||SGD DESC:Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis Gene:AXL1(YPR122W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BRE1(YDL074C)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BUD28(YLR062C_d)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CIN1(YOR349W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CLC1(YGR167W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG7(YGL005C)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ4(YDR204W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex Gene:COX23(YHR116W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:FIG4(YNL325C)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FRE3(YOR381W)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GCN3(YKR026C)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GET1(YGL020C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GPD1(YDL022W)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GPR1(YDL035C)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HTL1(YCR020W-B)|FD-Score:-3.86|P-value:5.72E-5||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:ICP55(YER078C)|FD-Score:-5.35|P-value:4.45E-8||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:MDL2(YPL270W)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MLH2(YLR035C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:RNH201(YNL072W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL13B(YMR142C)|FD-Score:-4.98|P-value:3.10E-7||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:5.47|P-value:2.31E-8||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RXT2(YBR095C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SCJ1(YMR214W)|FD-Score:-4.57|P-value:2.50E-6||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SLZ1(YNL196C_p)|FD-Score:5.4|P-value:3.35E-8||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:TDP1(YBR223C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TOR1(YJR066W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:VOA1(YGR106C)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:XRS2(YDR369C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBR292C(YBR292C_d)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YGR250C(YGR250C)|FD-Score:-4.07|P-value:2.33E-5||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YGR290W(YGR290W_d)|FD-Score:5.7|P-value:6.00E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YHR080C(YHR080C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIL025C(YIL025C_d)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR031W(YLR031W_p)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Putative protein of unknown function Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL120C(YNL120C_d)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNL146C-A(YNL146C-A_p)|FD-Score:6.04|P-value:7.92E-10||SGD DESC:Putative protein of unknown function Gene:YNL266W(YNL266W_d)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YPL102C(YPL102C_d)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:ADE1(YAR015W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AMA1(YGR225W)|FD-Score:-3.74|P-value:9.24E-5||SGD DESC:Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis Gene:AXL1(YPR122W)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BRE1(YDL074C)|FD-Score:-3.55|P-value:1.92E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BUD28(YLR062C_d)|FD-Score:3.2|P-value:6.80E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CIN1(YOR349W)|FD-Score:3.1|P-value:9.80E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CLC1(YGR167W)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG7(YGL005C)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ4(YDR204W)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex Gene:COX23(YHR116W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs Gene:FIG4(YNL325C)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FRE3(YOR381W)|FD-Score:5.6|P-value:1.10E-8||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GCN3(YKR026C)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GET1(YGL020C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GPD1(YDL022W)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GPR1(YDL035C)|FD-Score:-3.21|P-value:6.58E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HTL1(YCR020W-B)|FD-Score:-3.86|P-value:5.72E-5||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:ICP55(YER078C)|FD-Score:-5.35|P-value:4.45E-8||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:MDL2(YPL270W)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis Gene:MLH2(YLR035C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:RNH201(YNL072W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPL13B(YMR142C)|FD-Score:-4.98|P-value:3.10E-7||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPS17A(YML024W)|FD-Score:3.32|P-value:4.44E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:5.47|P-value:2.31E-8||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RXT2(YBR095C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SCJ1(YMR214W)|FD-Score:-4.57|P-value:2.50E-6||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SLZ1(YNL196C_p)|FD-Score:5.4|P-value:3.35E-8||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:TDP1(YBR223C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topoisomerase I and topoisomerase II; mutations in human homolog result in the neurodegenerative disease SCANI Gene:TOR1(YJR066W)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:VOA1(YGR106C)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:XRS2(YDR369C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBR292C(YBR292C_d)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YGR250C(YGR250C)|FD-Score:-4.07|P-value:2.33E-5||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YGR290W(YGR290W_d)|FD-Score:5.7|P-value:6.00E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YHR080C(YHR080C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIL025C(YIL025C_d)|FD-Score:3.9|P-value:4.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR031W(YLR031W_p)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:Putative protein of unknown function Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.53|P-value:2.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL120C(YNL120C_d)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNL146C-A(YNL146C-A_p)|FD-Score:6.04|P-value:7.92E-10||SGD DESC:Putative protein of unknown function Gene:YNL266W(YNL266W_d)|FD-Score:4.74|P-value:1.05E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YPL102C(YPL102C_d)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR340C7.542.35E-143.65RPA43RNA polymerase I subunit A43
YBR087W3.895.07E-50.20RFC5Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YOR117W3.691.11E-40.20RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YNL038W3.502.35E-40.26GPI15Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein
YGR158C3.246.08E-40.07MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
YML130C3.177.70E-40.02ERO1Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
YDR396W_d3.158.13E-40.05YDR396W_dDubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
YDR367W3.119.49E-40.02KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YBL023C3.099.99E-40.06MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YLL050C3.030.001210.12COF1Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
YDL064W2.920.001780.04UBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
YPR105C2.880.002020.00COG4Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR170C2.870.002050.08SEC7Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
YMR005W2.790.002610.02TAF4TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
YGR267C2.770.002810.02FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL146C-A_p6.047.92E-10YNL146C-A_pPutative protein of unknown function
YGR290W_d5.706.00E-9YGR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
YOR381W5.601.10E-8FRE3Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
YOR014W5.472.31E-8RTS1B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
YNL196C_p5.403.35E-8SLZ1_pSporulation-specific protein with a leucine zipper motif
YNL266W_d4.741.05E-6YNL266W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C
YLR035C4.221.22E-5MLH2Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p
YHR116W4.211.26E-5COX23Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
YPL270W4.181.44E-5MDL2Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, required for respiratory growth at high temperature; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis
YIL025C_d3.904.81E-5YIL025C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR369C3.885.16E-5XRS2Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
YNL072W3.651.29E-4RNH201Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
YHR080C3.631.40E-4YHR080CProtein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication
YBR095C3.551.92E-4RXT2Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth
YMR052C-A_d3.532.04E-4YMR052C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1558
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0872.45E-11SGTC_14904089-0339 670.0 nMChemDiv (Drug-like library)7425420.148148
0.0807.02E-10SGTC_2731amoxapine 43.4 μMMiscellaneous21700.0434783
0.0782.45E-9SGTC_2420aureobasidin a 50.0 nMMiscellaneous99634300.0952381
0.0758.11E-9SGTC_21735763948 167.0 μMChembridge (Fragment library)28671770.145455tubulin folding & SWR complex
0.0722.77E-8SGTC_5660958-0068 16.5 μMChemDiv (Drug-like library)68153650.0958904RSC & ERG11
0.0723.53E-8SGTC_1588farnesol 29.9 μMTimTec (Pure natural product library)4450700.04
0.0707.07E-8SGTC_32129128307 49.5 μMChembridge (Drug-like library)272435420.0704225
0.0663.19E-7SGTC_10144130-3005 69.3 μMChemDiv (Drug-like library)7436080.0645161
0.0663.20E-7SGTC_1539benzyladenosine 56.0 μMTimTec (Pure natural product library)922080.0666667
0.0656.67E-7SGTC_8981000-0849 28.3 μMChemDiv (Drug-like library)67518070.0923077
0.0604.06E-6SGTC_1559coumarin 343 70.1 μMTimTec (Pure natural product library)1087700.0895522
0.0596.69E-6SGTC_1553gossypol 38.6 μMTimTec (Pure natural product library)35030.306122
0.0587.27E-6SGTC_2502avocadyne acetate 18.6 μMMicrosource (Natural product library)39520790.0655738
0.0571.38E-5SGTC_1602st001878 87.6 μMTimTec (Natural product derivative library)5954050.1
0.0561.82E-5SGTC_30699117009 49.5 μMChembridge (Drug-like library)419049810.044117660S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1553gossypol38.6 μM0.3061223503TimTec (Pure natural product library)518.55446.59268
SGTC_2516gossypol71.55 μM0.3061223503TimTec (Pure natural product library)518.55446.59268
SGTC_1554st06929938.7 μM0.25925924207999TimTec (Pure natural product library)516.584885.77368
SGTC_1550gossypolone36.6 μM0.2115385478622TimTec (Pure natural product library)546.521444.956410
SGTC_2507purpurin79.67 μM0.1956526683Microsource (Natural product library)256.210322.08235copper-dependent oxidative stress
SGTC_10064099-6547255 μM0.1929823146833ChemDiv (Drug-like library)287.3039063.94313
SGTC_2231micatex6.85 μM0.19047620737Miscellaneous344.8264.31911
SGTC_2541muurolladie-3-one83.07 μM0.1886793989419Microsource (Natural product library)218.334583.74301excess fatty acid
SGTC_25534-methylesculetin85.09 μM0.18755319502Microsource (Natural product library)192.168121.86224
SGTC_2608thymoquinone42.66 μM0.18604710281Microsource (Natural product library)164.201082.28802
SGTC_1569carminic acid40.6 μM0.18571414749TimTec (Pure natural product library)492.3864-0.436913