coumarin 343

A hepatotoxic, DNA x-linker.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1559
Screen concentration 70.1 μM
Source TimTec (Pure natural product library)
PubChem CID 108770
SMILES C1CC2=C3C(=C4C(=C2)C=C(C(=O)O4)C(=O)O)CCCN3C1
Standardized SMILES OC(=O)C1=Cc2cc3CCCN4CCCc(c2OC1=O)c34
Molecular weight 285.2946
ALogP 2.71
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.61
% growth inhibition (Hom. pool) 2.92


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 108770
Download HIP data (tab-delimited text)  (excel)
Gene:CBK1(YNL161W)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.04||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CLP1(YOR250C)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.11||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:ERG29(YMR134W_p)|FD-Score:3.22|P-value:6.49E-4|Clearance:0.03||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:KEI1(YDR367W)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.02||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LSM4(YER112W)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:ORC4(YPR162C)|FD-Score:-3.18|P-value:7.41E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PRT1(YOR361C)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.22||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RET2(YFR051C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.19||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPP1(YHR062C)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SFH1(YLR321C)|FD-Score:-3.19|P-value:7.10E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SPC42(YKL042W)|FD-Score:4.46|P-value:4.10E-6|Clearance:0.78||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane Gene:CBK1(YNL161W)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.04||SGD DESC:Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress Gene:CLP1(YOR250C)|FD-Score:3.18|P-value:7.26E-4|Clearance:0.11||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:ERG29(YMR134W_p)|FD-Score:3.22|P-value:6.49E-4|Clearance:0.03||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:KEI1(YDR367W)|FD-Score:3.23|P-value:6.15E-4|Clearance:0.02||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:LSM4(YER112W)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:ORC4(YPR162C)|FD-Score:-3.18|P-value:7.41E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:PRT1(YOR361C)|FD-Score:3.45|P-value:2.78E-4|Clearance:0.22||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RET2(YFR051C)|FD-Score:3.64|P-value:1.35E-4|Clearance:0.19||SGD DESC:Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER Gene:RPP1(YHR062C)|FD-Score:-3.67|P-value:1.22E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SFH1(YLR321C)|FD-Score:-3.19|P-value:7.10E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SPC42(YKL042W)|FD-Score:4.46|P-value:4.10E-6|Clearance:0.78||SGD DESC:Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 108770
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:-3.9|P-value:4.82E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:API2(YDR525W_d)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APL6(YGR261C)|FD-Score:-4.52|P-value:3.07E-6||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ARF1(YDL192W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ASE1(YOR058C)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:CBP1(YJL209W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CIN4(YMR138W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CIN8(YEL061C)|FD-Score:5.03|P-value:2.39E-7||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:EAF7(YNL136W)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:HAL1(YPR005C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:LSM7(YNL147W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:NEW1(YPL226W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OPI1(YHL020C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OPI7(YDR360W_d)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:OYE2(YHR179W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PAH1(YMR165C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PEP3(YLR148W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PRM1(YNL279W)|FD-Score:4.36|P-value:6.54E-6||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PUS7(YOR243C)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RTK1(YDL025C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SDH4(YDR178W)|FD-Score:4.96|P-value:3.59E-7||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SEA4(YBL104C)|FD-Score:4.89|P-value:5.03E-7||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SHM2(YLR058C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SIN3(YOL004W)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SPI1(YER150W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPR3(YGR059W)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:STM1(YLR150W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SWI3(YJL176C)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TAE2(YPL009C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:THR1(YHR025W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TYW3(YGL050W)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UBX4(YMR067C)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VPS30(YPL120W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:XRS2(YDR369C)|FD-Score:3.87|P-value:5.36E-5||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBR051W(YBR051W_d)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR219C(YBR219C_p)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YDJ1(YNL064C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family Gene:YEL020C(YEL020C_p)|FD-Score:3.86|P-value:5.56E-5||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YGR290W(YGR290W_d)|FD-Score:4.33|P-value:7.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YKL091C(YKL091C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR444C(YLR444C_d)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR097C(YOR097C_p)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YOR131C(YOR131C_p)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YPL152W-A(YPL152W-A_p)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:ACB1(YGR037C)|FD-Score:-3.9|P-value:4.82E-5||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:API2(YDR525W_d)|FD-Score:4.14|P-value:1.77E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:APL6(YGR261C)|FD-Score:-4.52|P-value:3.07E-6||SGD DESC:Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools Gene:ARF1(YDL192W)|FD-Score:-3.23|P-value:6.14E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication Gene:ASE1(YOR058C)|FD-Score:-3.3|P-value:4.89E-4||SGD DESC:Mitotic spindle midzone localized protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress Gene:CBP1(YJL209W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CIN4(YMR138W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CIN8(YEL061C)|FD-Score:5.03|P-value:2.39E-7||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:EAF7(YNL136W)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A Gene:HAL1(YPR005C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:LSM7(YNL147W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:NEW1(YPL226W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction Gene:OPI1(YHL020C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OPI7(YDR360W_d)|FD-Score:3.73|P-value:9.47E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:OYE2(YHR179W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PAH1(YMR165C)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2 Gene:PEP3(YLR148W)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PRM1(YNL279W)|FD-Score:4.36|P-value:6.54E-6||SGD DESC:Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p Gene:PUS7(YOR243C)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:RTK1(YDL025C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress Gene:SDH4(YDR178W)|FD-Score:4.96|P-value:3.59E-7||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SEA4(YBL104C)|FD-Score:4.89|P-value:5.03E-7||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SHM2(YLR058C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SIN3(YOL004W)|FD-Score:4.53|P-value:3.00E-6||SGD DESC:Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity Gene:SPI1(YER150W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPR3(YGR059W)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:STM1(YLR150W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:SWI3(YJL176C)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions Gene:TAE2(YPL009C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein of unknown function, involved in protein translation; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:THR1(YHR025W)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TYW3(YGL050W)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UBX4(YMR067C)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VPS30(YPL120W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:XRS2(YDR369C)|FD-Score:3.87|P-value:5.36E-5||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBR051W(YBR051W_d)|FD-Score:3.97|P-value:3.56E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase Gene:YBR219C(YBR219C_p)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YDJ1(YNL064C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family Gene:YEL020C(YEL020C_p)|FD-Score:3.86|P-value:5.56E-5||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YGR290W(YGR290W_d)|FD-Score:4.33|P-value:7.40E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YKL091C(YKL091C)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Putative homolog of Sec14p, which is a phosphatidylinositol/phosphatidylcholine transfer protein involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study Gene:YLR444C(YLR444C_d)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR097C(YOR097C_p)|FD-Score:4.44|P-value:4.52E-6||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YOR131C(YOR131C_p)|FD-Score:4.41|P-value:5.11E-6||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YPL152W-A(YPL152W-A_p)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL042W4.464.10E-60.78SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YNL161W3.681.18E-40.04CBK1Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
YFR051C3.641.35E-40.19RET2Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
YOR361C3.452.78E-40.22PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
YDR367W3.236.15E-40.02KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YMR134W_p3.226.49E-40.03ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YOR250C3.187.26E-40.11CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YDL126C3.070.001070.01CDC48AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
YMR033W3.060.001100.07ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YDR356W2.990.001380.00SPC110Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
YGL112C2.990.001380.17TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YDR228C2.820.002420.14PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YBR004C2.680.003650.01GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YGR002C2.680.003730.02SWC4Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex
YOL097C2.650.003970.10WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL061C5.032.39E-7CIN8Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
YDR178W4.963.59E-7SDH4Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain
YBL104C4.895.03E-7SEA4Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites
YOL004W4.533.00E-6SIN3Component of the Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
YOR097C_p4.444.52E-6YOR097C_pPutative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene
YOR131C_p4.415.11E-6YOR131C_pPutative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress
YNL279W4.366.54E-6PRM1Pheromone-regulated multispanning membrane protein involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p
YGR290W_d4.337.40E-6YGR290W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
YHR025W4.261.03E-5THR1Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
YDL025C4.261.04E-5RTK1Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
YNL136W4.251.05E-5EAF7Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A
YDR525W_d4.141.77E-5API2_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
YBR051W_d3.973.56E-5YBR051W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partiallly overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase
YDR369C3.875.36E-5XRS2Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
YEL020C_p3.865.56E-5YEL020C_pProtein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data

GO enrichment analysis for SGTC_1559
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0944.92E-13SGTC_370566-0070 1.6 μMChemDiv (Drug-like library)16266860.078125
0.0831.97E-10SGTC_460329-0187a 2.2 μMChemDiv (Drug-like library)464948170.102941
0.0732.24E-8SGTC_1070358-0022 2.7 μMChemDiv (Drug-like library)5211060.0491803SWF1 & branched chain AA biosynthesis
0.0722.50E-8SGTC_162k030-0015 37.8 μMChemDiv (Drug-like library)18099200.0819672iron homeostasis
0.0649.99E-7SGTC_10523253-3998 198.0 μMChemDiv (Drug-like library)54503170.0813954
0.0604.08E-6SGTC_900870-0863 4.6 μMChemDiv (Drug-like library)17381640.0789474
0.0604.06E-6SGTC_1558st069300 45.6 μMTimTec (Pure natural product library)242080000.0895522
0.0604.40E-6SGTC_1920st075728 45.7 μMTimTec (Natural product derivative library)34003060.192308
0.0571.33E-5SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.05TRP & mitochondrial translation
0.0571.33E-5SGTC_2420aureobasidin a 50.0 nMMiscellaneous99634300.0862069
0.0561.52E-5SGTC_1860st056228 74.6 μMTimTec (Natural product derivative library)6886790.128571
0.0552.11E-5SGTC_32939120939 62.0 μMChembridge (Drug-like library)9340870.0243902
0.0525.98E-5SGTC_30499092223 49.5 μMChembridge (Drug-like library)86239170.121622
0.0526.83E-5SGTC_22117117706 200.0 μMChembridge (Fragment library)8227710.112903
0.0519.52E-5SGTC_12072099-0101 141.0 μMChemDiv (Drug-like library)58373810.0714286

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1936st07440774.3 μM0.64809813TimTec (Natural product derivative library)269.29522.73504DNA damage response
SGTC_1600coumarin 10671.1 μM0.519231100336TimTec (Pure natural product library)281.348964.10103
SGTC_1628st00425277.7 μM0.253968692968TimTec (Natural product derivative library)257.28452.4713
SGTC_1821st05346565.6 μM0.241935675086TimTec (Natural product derivative library)282.090121.81613
SGTC_1871st05744867.3 μM0.230769691747TimTec (Natural product derivative library)297.101462.81204
SGTC_23296138490200 μM0.229508720019Chembridge (Fragment library)231.247222.12914
SGTC_2414st07723292.9 μM0.229508729801TimTec (Natural product derivative library)215.247823.13622
SGTC_399oxolinic acid75 μM0.2272734628Miscellaneous261.230141.27316
SGTC_1914st06236772.6 μM0.223881978205TimTec (Natural product derivative library)275.299781.98214
SGTC_1910st06235260.4 μM0.2162162245730TimTec (Natural product derivative library)287.310481.94514