lithocholic acid

(4R)-4-[(3R,5R,8R,9S,10S,13R,14S,17R)-3-hydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoic acid

A bile acid which acts as a detergent.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1560
Screen concentration 53.1 μM
Source TimTec (Pure natural product library)
PubChem CID 9903
SMILES CC(CCC(=O)O)C1CCC2C1(CCC3C2CCC4C3(CCC(C4)O)C)C
Standardized SMILES CC(CCC(=O)O)C1CCC2C3CCC4CC(O)CCC4(C)C3CCC12C
Molecular weight 376.5726
ALogP 5.18
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.61
% growth inhibition (Hom. pool) 3.98


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 9903
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:4.57|P-value:2.41E-6|Clearance:0.62||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ATP16(YDL004W)|FD-Score:-3.38|P-value:3.69E-4|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:COG2(YGR120C)|FD-Score:-3.21|P-value:6.61E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:PKC1(YBL105C)|FD-Score:-4.87|P-value:5.50E-7|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIX7(YLL034C)|FD-Score:4.83|P-value:6.75E-7|Clearance:0.62||SGD DESC:Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions Gene:RPO26(YPR187W)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RSC6(YCR052W)|FD-Score:-4.97|P-value:3.38E-7|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC9(YML127W)|FD-Score:-3.83|P-value:6.40E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC13(YLR208W)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.16||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SUP45(YBR143C)|FD-Score:-3.43|P-value:3.02E-4|Clearance:0||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TID3(YIL144W)|FD-Score:3.49|P-value:2.46E-4|Clearance:0.46||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TIF35(YDR429C)|FD-Score:3.95|P-value:3.84E-5|Clearance:0.31||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:ACT1(YFL039C)|FD-Score:4.57|P-value:2.41E-6|Clearance:0.62||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ATP16(YDL004W)|FD-Score:-3.38|P-value:3.69E-4|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:COG2(YGR120C)|FD-Score:-3.21|P-value:6.61E-4|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:PKC1(YBL105C)|FD-Score:-4.87|P-value:5.50E-7|Clearance:0||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIX7(YLL034C)|FD-Score:4.83|P-value:6.75E-7|Clearance:0.62||SGD DESC:Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions Gene:RPO26(YPR187W)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RSC6(YCR052W)|FD-Score:-4.97|P-value:3.38E-7|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC9(YML127W)|FD-Score:-3.83|P-value:6.40E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC13(YLR208W)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.16||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SUP45(YBR143C)|FD-Score:-3.43|P-value:3.02E-4|Clearance:0||SGD DESC:Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p Gene:TID3(YIL144W)|FD-Score:3.49|P-value:2.46E-4|Clearance:0.46||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TIF35(YDR429C)|FD-Score:3.95|P-value:3.84E-5|Clearance:0.31||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 9903
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:BDF1(YLR399C)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:DSS1(YMR287C)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:GEA2(YEL022W)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GGC1(YDL198C)|FD-Score:-3.9|P-value:4.82E-5||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GND1(YHR183W)|FD-Score:-4.01|P-value:3.10E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HER2(YMR293C)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HXT3(YDR345C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:IES6(YEL044W)|FD-Score:-4.4|P-value:5.40E-6||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:KAP123(YER110C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:MHR1(YDR296W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MRPL24(YMR193W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL38(YKL170W)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MTG1(YMR097C)|FD-Score:-3.81|P-value:6.90E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MUB1(YMR100W)|FD-Score:3.81|P-value:7.07E-5||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NHA1(YLR138W)|FD-Score:4.82|P-value:7.34E-7||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:NPY1(YGL067W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member Gene:NVJ1(YHR195W)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:ROM2(YLR371W)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPS7A(YOR096W)|FD-Score:-3.81|P-value:7.00E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RTT109(YLL002W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:RXT2(YBR095C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SLM1(YIL105C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SUV3(YPL029W)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:UBR2(YLR024C)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:UBX4(YMR067C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VPS1(YKR001C)|FD-Score:-3.92|P-value:4.44E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS63(YLR261C_d)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR278C(YDR278C_d)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR011C(YJR011C_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.85|P-value:5.97E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Putative protein of unknown function Gene:YNR062C(YNR062C_p)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Putative membrane protein of unknown function Gene:YOR041C(YOR041C_d)|FD-Score:6.44|P-value:5.96E-11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance Gene:AFT1(YGL071W)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:BDF1(YLR399C)|FD-Score:-3.1|P-value:9.62E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:DSS1(YMR287C)|FD-Score:-3.22|P-value:6.33E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:GEA2(YEL022W)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GGC1(YDL198C)|FD-Score:-3.9|P-value:4.82E-5||SGD DESC:Mitochondrial GTP/GDP transporter, essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family Gene:GND1(YHR183W)|FD-Score:-4.01|P-value:3.10E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HER2(YMR293C)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HXT3(YDR345C)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:IES6(YEL044W)|FD-Score:-4.4|P-value:5.40E-6||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:KAP123(YER110C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:MHR1(YDR296W)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress Gene:MRPL24(YMR193W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene Gene:MRPL38(YKL170W)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MTG1(YMR097C)|FD-Score:-3.81|P-value:6.90E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MUB1(YMR100W)|FD-Score:3.81|P-value:7.07E-5||SGD DESC:MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene Gene:NHA1(YLR138W)|FD-Score:4.82|P-value:7.34E-7||SGD DESC:Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH Gene:NPY1(YGL067W)|FD-Score:3.12|P-value:9.10E-4||SGD DESC:NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member Gene:NVJ1(YHR195W)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:ROM2(YLR371W)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPS7A(YOR096W)|FD-Score:-3.81|P-value:7.00E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication Gene:RTT109(YLL002W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:RXT2(YBR095C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SLM1(YIL105C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication Gene:SUV3(YPL029W)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:UBR2(YLR024C)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:UBX4(YMR067C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress Gene:VPS1(YKR001C)|FD-Score:-3.92|P-value:4.44E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS63(YLR261C_d)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL071C-B(YBL071C-B_p)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR278C(YDR278C_d)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR011C(YJR011C_p)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.85|P-value:5.97E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YNL146C-A(YNL146C-A_p)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Putative protein of unknown function Gene:YNR062C(YNR062C_p)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Putative membrane protein of unknown function Gene:YOR041C(YOR041C_d)|FD-Score:6.44|P-value:5.96E-11||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLL034C4.836.75E-70.62RIX7Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions
YFL039C4.572.41E-60.62ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YDR429C3.953.84E-50.31TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YLR208W3.641.36E-40.16SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YIL144W3.492.46E-40.46TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YGL150C3.020.001250.09INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YML085C2.930.001680.08TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YKL014C2.850.002200.14URB1Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit
YMR059W2.710.003400.04SEN15Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
YDR416W2.660.003850.05SYF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
YPL085W2.610.004470.02SEC16COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
YDR302W2.590.004740.15GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YDR267C2.450.007240.02CIA1Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; contains WD40 repeats; ortholog of human Ciao1 protein
YKL013C2.420.007710.02ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YDR308C2.410.008060.01SRB7Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR041C_d6.445.96E-11YOR041C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance
YLR138W4.827.34E-7NHA1Na+/H+ antiporter involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH
YHR195W4.022.91E-5NVJ1Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)
YLR024C3.934.29E-5UBR2Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex
YKL096C-B_p3.855.97E-5YKL096C-B_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YMR100W3.817.07E-5MUB1MYND domain-containing protein required for ubiquitination and turnover of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; similar to the A. nidulans samB gene
YIL105C3.651.32E-4SLM1Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
YJR011C_p3.442.91E-4YJR011C_pPutative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS
YNL146C-A_p3.373.73E-4YNL146C-A_pPutative protein of unknown function
YDR345C3.363.96E-4HXT3Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication
YGL071W3.295.01E-4AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YBL008W-A_p3.158.27E-4YBL008W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YGL067W3.129.10E-4NPY1NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; nudix hydrolase family member
YDR278C_d3.119.24E-4YDR278C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YOL029C_p3.080.00103YOL029C_pPutative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens

GO enrichment analysis for SGTC_1560
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1263.38E-22SGTC_15615-methoxyflavone 52.9 μMTimTec (Pure natural product library)945250.025641
0.1202.78E-20SGTC_1555citronellol 128.0 μMTimTec (Pure natural product library)88420.114754
0.1054.28E-16SGTC_1602st001878 87.6 μMTimTec (Natural product derivative library)5954050.0519481
0.1055.48E-16SGTC_32009114385 49.5 μMChembridge (Drug-like library)170180300.0512821
0.0944.07E-13SGTC_28969052656 71.4 μMChembridge (Drug-like library)64694360.0581395endomembrane recycling
0.0945.90E-13SGTC_20255147754 141.0 μMChembridge (Fragment library)3157770.0526316
0.0938.13E-13SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.025974
0.0912.64E-12SGTC_1553gossypol 38.6 μMTimTec (Pure natural product library)35030.0675676
0.0898.01E-12SGTC_7864544-0069 423.0 μMChemDiv (Drug-like library)7488210.04
0.0881.10E-11SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.0493827
0.0881.55E-11SGTC_1616st002734 56.1 μMTimTec (Natural product derivative library)40261010.2
0.0841.12E-10SGTC_1743st037606 46.4 μMTimTec (Natural product derivative library)5901420.0361446
0.0814.41E-10SGTC_1764st044502 93.5 μMTimTec (Natural product derivative library)84070.0142857
0.0765.41E-9SGTC_21986589944 200.0 μMChembridge (Fragment library)52902680.0243902
0.0765.54E-9SGTC_890302-0250 142.1 μMChemDiv (Drug-like library)42035180.056338

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2687chenodiol100 μM0.7547175702181Microsource (Natural product library)392.5724.01434
SGTC_2621lithochol-11-enic acid100 μM0.6779666708711Microsource (Natural product library)374.556724.73923
SGTC_1517deoxycholic acid methyl ester49.2 μM0.622951229346TimTec (Pure natural product library)406.598584.30724
SGTC_2531methyl deoxycholate70.07 μM0.62295116667744Microsource (Natural product library)406.598584.30724
SGTC_2555cholic acid100 μM0.4925376708657Microsource (Natural product library)422.597983.13835
SGTC_1572epiandrosterone68.9 μM0.473684441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.473684441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.4736842754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_2628lathosterol100 μM0.388066710616Microsource (Natural product library)386.653547.37611
SGTC_1974st06944826.9 μM0.3333336323587TimTec (Natural product derivative library)305.454943.36923
SGTC_1750st0818592.36 μM0.3275865291695TimTec (Natural product derivative library)275.472024.43511endomembrane recycling