5-methoxyflavone

5-methoxy-2-phenylchromen-4-one

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1561
Screen concentration 52.9 μM
Source TimTec (Pure natural product library)
PubChem CID 94525
SMILES COC1=CC=CC2=C1C(=O)C=C(O2)C3=CC=CC=C3
Standardized SMILES COc1cccc2OC(=CC(=O)c12)c3ccccc3
Molecular weight 252.2647
ALogP 3.12
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.13
% growth inhibition (Hom. pool) 8.13


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 94525
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:4.04|P-value:2.71E-5|Clearance:1.03||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP9(YMR033W)|FD-Score:-3.66|P-value:1.25E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC5(YMR001C)|FD-Score:4.45|P-value:4.27E-6|Clearance:1.03||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:CWC2(YDL209C)|FD-Score:-3.1|P-value:9.71E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:NUP49(YGL172W)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:RPC17(YJL011C)|FD-Score:-4.59|P-value:2.26E-6|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPL10(YLR075W)|FD-Score:-3.12|P-value:8.93E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL5(YPL131W)|FD-Score:-3.13|P-value:8.83E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP0(YLR340W)|FD-Score:-3.95|P-value:3.88E-5|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RRP9(YPR137W)|FD-Score:-4.08|P-value:2.22E-5|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC6(YCR052W)|FD-Score:-3.62|P-value:1.46E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC13(YLR208W)|FD-Score:4.55|P-value:2.70E-6|Clearance:1.03||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:YGR114C(YGR114C_d)|FD-Score:-3.65|P-value:1.30E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:ACT1(YFL039C)|FD-Score:4.04|P-value:2.71E-5|Clearance:1.03||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ARP9(YMR033W)|FD-Score:-3.66|P-value:1.25E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC5(YMR001C)|FD-Score:4.45|P-value:4.27E-6|Clearance:1.03||SGD DESC:Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate Gene:CWC2(YDL209C)|FD-Score:-3.1|P-value:9.71E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:NUP49(YGL172W)|FD-Score:-3.27|P-value:5.45E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:RPC17(YJL011C)|FD-Score:-4.59|P-value:2.26E-6|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPL10(YLR075W)|FD-Score:-3.12|P-value:8.93E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL5(YPL131W)|FD-Score:-3.13|P-value:8.83E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP0(YLR340W)|FD-Score:-3.95|P-value:3.88E-5|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RRP9(YPR137W)|FD-Score:-4.08|P-value:2.22E-5|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:RSC6(YCR052W)|FD-Score:-3.62|P-value:1.46E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC13(YLR208W)|FD-Score:4.55|P-value:2.70E-6|Clearance:1.03||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:YGR114C(YGR114C_d)|FD-Score:-3.65|P-value:1.30E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 94525
Download HOP data (tab-delimited text)  (excel)
Gene:ADK2(YER170W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AIM36(YMR157C)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ARL1(YBR164C)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:BRE1(YDL074C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BTS1(YPL069C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CKA1(YIL035C)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CKI1(YLR133W)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CLN3(YAL040C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:DAL81(YIR023W)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:FAR10(YLR238W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:GAL4(YPL248C)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GND1(YHR183W)|FD-Score:-4.63|P-value:1.82E-6||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HTL1(YCR020W-B)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IES3(YLR052W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRS4(YKR019C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:PEX19(YDL065C)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PEX25(YPL112C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p Gene:PKR1(YMR123W)|FD-Score:-3.73|P-value:9.43E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:ROM2(YLR371W)|FD-Score:-4.15|P-value:1.69E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:SMA2(YML066C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:STO1(YMR125W)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:TAT1(YBR069C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THI2(YBR240C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TRP2(YER090W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.72|P-value:5.31E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:YBR141C(YBR141C_p)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YEL014C(YEL014C_d)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL043W(YJL043W_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YKR023W(YKR023W_p)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL106W(YOL106W_d)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR097C(YOR097C_p)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YOR200W(YOR200W_d)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPK1(YKL126W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YSC84(YHR016C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:ADK2(YER170W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:AIM36(YMR157C)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies Gene:ARL1(YBR164C)|FD-Score:3.94|P-value:4.15E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:BRE1(YDL074C)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:E3 ubiquitin ligase, forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control Gene:BTS1(YPL069C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CKA1(YIL035C)|FD-Score:-3.6|P-value:1.58E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CKI1(YLR133W)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CLN3(YAL040C)|FD-Score:-3.45|P-value:2.82E-4||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:DAL81(YIR023W)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:FAR10(YLR238W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate Gene:GAL4(YPL248C)|FD-Score:3.23|P-value:6.16E-4||SGD DESC:DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p Gene:GND1(YHR183W)|FD-Score:-4.63|P-value:1.82E-6||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HTL1(YCR020W-B)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:IES3(YLR052W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IRS4(YKR019C)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:PEX19(YDL065C)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PEX25(YPL112C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p Gene:PKR1(YMR123W)|FD-Score:-3.73|P-value:9.43E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:ROM2(YLR371W)|FD-Score:-4.15|P-value:1.69E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:SMA2(YML066C)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation Gene:STO1(YMR125W)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:TAT1(YBR069C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:THI2(YBR240C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type Gene:TRP2(YER090W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.72|P-value:5.31E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:YBR141C(YBR141C_p)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YEL014C(YEL014C_d)|FD-Score:3.73|P-value:9.73E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL043W(YJL043W_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function; YJL043W is a non-essential gene Gene:YKR023W(YKR023W_p)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL106W(YOL106W_d)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR097C(YOR097C_p)|FD-Score:-3.38|P-value:3.62E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YOR200W(YOR200W_d)|FD-Score:-3.21|P-value:6.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPK1(YKL126W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YSC84(YHR016C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W4.552.70E-61.03SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YMR001C4.454.27E-61.03CDC5Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate
YFL039C4.042.71E-51.03ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YEL002C3.010.001320.02WBP1Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
YML010W2.980.001420.00SPT5Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair
YLL034C2.980.001430.04RIX7Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions
YOL123W2.940.001649.19E-4HRP1Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences
YGL099W2.940.001650.08LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YBR123C2.860.002120.03TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YNL189W2.830.002320.07SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YJL014W2.760.002860.12CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGL111W2.640.004160.02NSA1Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
YJR006W2.620.004390.02POL31Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair
YJR013W2.600.004630.03GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YLL018C2.580.005000.07DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C5.725.31E-9TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YOL106W_d4.711.26E-6YOL106W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR141C_p4.493.52E-6YBR141C_pPutative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
YIR023W4.444.41E-6DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YER170W4.201.33E-5ADK2Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background
YPL112C4.111.97E-5PEX25Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p
YBR164C3.944.15E-5ARL1Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
YLR238W3.787.84E-5FAR10Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate
YEL014C_d3.739.73E-5YEL014C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YER090W3.651.31E-4TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YJL043W_p3.452.83E-4YJL043W_pPutative protein of unknown function; YJL043W is a non-essential gene
YHR016C3.403.36E-4YSC84Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication
YBR069C3.304.76E-4TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YPL248C3.236.16E-4GAL4DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p
YKR019C3.148.38E-4IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1561
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2293.42E-71SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.422222
0.2216.19E-66SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.107692
0.2038.56E-56SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.423077
0.1962.94E-52SGTC_10583448-5425 97.3 μMChemDiv (Drug-like library)72797050.178571
0.1945.00E-51SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0952381
0.1932.34E-50SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.129032
0.1922.35E-50SGTC_1710st031758 61.5 μMTimTec (Natural product derivative library)53943410.157143
0.1922.41E-50SGTC_24605552655 174.2 μMMiscellaneous54019890.09375amide catabolism
0.1891.59E-48SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.0666667
0.1885.69E-48SGTC_11941488-0194 225.0 μMChemDiv (Drug-like library)13914520.0434783
0.1762.84E-42SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.288462
0.1742.85E-41SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.152542
0.1736.49E-41SGTC_14204'-methoxyflavone 42.0 μMChemDiv (Drug-like library)777930.522727
0.1723.70E-40SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.037037
0.1713.89E-40SGTC_2409alverine citrate 64.0 μMMiscellaneous217180.14

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_15924',5'-dihydroxyflavone8.85 μM0.794872688669TimTec (Pure natural product library)282.290663.1030460S ribosome export
SGTC_1861st05764717.4 μM0.756098688668TimTec (Natural product derivative library)282.290663.10304
SGTC_15892',5'-dimethoxyflavone47.2 μM0.74359688667TimTec (Pure natural product library)282.290663.10304
SGTC_2550chrysin dimethyl ether71.87 μM0.69047688881Microsource (Natural product library)282.290663.10304
SGTC_1852st05625431.4 μM0.651163442583TimTec (Natural product derivative library)312.316643.08705
SGTC_1834st05598152.8 μM0.636364471719TimTec (Natural product derivative library)284.263482.63625RPP1 & pyrimidine depletion
SGTC_1848st05600316.4 μM0.547619146492TimTec (Natural product derivative library)252.264683.1203
SGTC_1840st05536371 μM0.533333676033TimTec (Natural product derivative library)282.290663.10304
SGTC_14204'-methoxyflavone42 μM0.52272777793ChemDiv (Drug-like library)252.264683.1203
SGTC_26344'-methoxyflavone100 μM0.52272777793Microsource (Natural product library)252.264683.1203
SGTC_1857st05600779.3 μM0.521739619834TimTec (Natural product derivative library)252.264683.1203