conessine

An anti-amoebic, histamine inhibitor.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1563
Screen concentration 56.1 μM
Source TimTec (Pure natural product library)
PubChem CID 441082
SMILES CC1C2CCC3C2(CCC4C3CC=C5C4(CCC(C5)N(C)C)C)CN1C
Standardized SMILES CC1C2CCC3C4CC=C5CC(CCC5(C)C4CCC23CN1C)N(C)C
Molecular weight 356.5878
ALogP 4.43
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.89
% growth inhibition (Hom. pool) -2.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 441082
Download HIP data (tab-delimited text)  (excel)
Gene:BET4(YJL031C)|FD-Score:-3.69|P-value:1.12E-4|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CCT4(YDL143W)|FD-Score:3.91|P-value:4.70E-5|Clearance:0.1||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:PCF11(YDR228C)|FD-Score:-3.54|P-value:2.00E-4|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PRE1(YER012W)|FD-Score:-4.13|P-value:1.85E-5|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:SLN1(YIL147C)|FD-Score:-4.08|P-value:2.21E-5|Clearance:0||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:SRP72(YPL210C)|FD-Score:-3.3|P-value:4.84E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TAF7(YMR227C)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TEL2(YGR099W)|FD-Score:3.81|P-value:7.00E-5|Clearance:0.9||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TSR1(YDL060W)|FD-Score:5.03|P-value:2.46E-7|Clearance:1.12||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:USE1(YGL098W)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p Gene:BET4(YJL031C)|FD-Score:-3.69|P-value:1.12E-4|Clearance:0||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CCT4(YDL143W)|FD-Score:3.91|P-value:4.70E-5|Clearance:0.1||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:PCF11(YDR228C)|FD-Score:-3.54|P-value:2.00E-4|Clearance:0||SGD DESC:mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping Gene:PRE1(YER012W)|FD-Score:-4.13|P-value:1.85E-5|Clearance:0||SGD DESC:Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle Gene:SLN1(YIL147C)|FD-Score:-4.08|P-value:2.21E-5|Clearance:0||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:SRP72(YPL210C)|FD-Score:-3.3|P-value:4.84E-4|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TAF7(YMR227C)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TEL2(YGR099W)|FD-Score:3.81|P-value:7.00E-5|Clearance:0.9||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TSR1(YDL060W)|FD-Score:5.03|P-value:2.46E-7|Clearance:1.12||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:USE1(YGL098W)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:Essential SNARE protein localized to the ER, involved in retrograde traffic from the Golgi to the ER; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 441082
Download HOP data (tab-delimited text)  (excel)
Gene:ADE12(YNL220W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AIM7(YDR063W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss Gene:ARF3(YOR094W)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity Gene:ATE1(YGL017W)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:BDH1(YAL060W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:COQ2(YNR041C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:CST9(YLR394W)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:DOS2(YDR068W)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:HTB2(YBL002W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IMP1(YMR150C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IPK1(YDR315C)|FD-Score:5.78|P-value:3.68E-9||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IRC21(YMR073C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KEX1(YGL203C)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KTR3(YBR205W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress Gene:LEU1(YGL009C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway Gene:MET13(YGL125W)|FD-Score:3.81|P-value:6.95E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MLH3(YPL164C)|FD-Score:-3.75|P-value:8.74E-5||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MRP2(YPR166C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSG5(YNL053W)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MTH1(YDR277C)|FD-Score:5.26|P-value:7.14E-8||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:MUS81(YDR386W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NGL3(YML118W)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication Gene:OGG1(YML060W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OSH2(YDL019C)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEF1(YGR058W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PKR1(YMR123W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PRS5(YOL061W)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:RDR1(YOR380W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:RPN13(YLR421C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:SAM2(YDR502C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SCO2(YBR024W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SEF1(YBL066C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Putative transcription factor, has homolog in Kluyveromyces lactis Gene:SNF2(YOR290C)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNT2(YGL131C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SPS1(YDR523C)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis Gene:SSM4(YIL030C)|FD-Score:3.23|P-value:6.08E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:SUB1(YMR039C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:THI3(YDL080C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:THP2(YHR167W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TOM1(YDR457W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:YAL056C-A(YAL056C-A_d)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR300C(YBR300C_d)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDL073W(YDL073W_p)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDL162C(YDL162C_d)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR306C(YDR306C_p)|FD-Score:9.06|P-value:6.60E-20||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YET3(YDL072C)|FD-Score:4.9|P-value:4.70E-7||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGL118C(YGL118C_d)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR201C(YGR201C_p)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Putative protein of unknown function Gene:YKL030W(YKL030W_d)|FD-Score:4.83|P-value:6.87E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR287C(YLR287C_p)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YME2(YMR302C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YMR193C-A(YMR193C-A_d)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADE12(YNL220W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AIM7(YDR063W)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Protein that interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss Gene:ARF3(YOR094W)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity Gene:ATE1(YGL017W)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:BDH1(YAL060W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source Gene:COQ2(YNR041C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:CST9(YLR394W)|FD-Score:3.91|P-value:4.55E-5||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:DOS2(YDR068W)|FD-Score:4.54|P-value:2.86E-6||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:HTB2(YBL002W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IMP1(YMR150C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IPK1(YDR315C)|FD-Score:5.78|P-value:3.68E-9||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IRC21(YMR073C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KEX1(YGL203C)|FD-Score:4.74|P-value:1.08E-6||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KTR3(YBR205W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress Gene:LEU1(YGL009C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway Gene:MET13(YGL125W)|FD-Score:3.81|P-value:6.95E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MLH3(YPL164C)|FD-Score:-3.75|P-value:8.74E-5||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MRP2(YPR166C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSG5(YNL053W)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p Gene:MTH1(YDR277C)|FD-Score:5.26|P-value:7.14E-8||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:MUS81(YDR386W)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NGL3(YML118W)|FD-Score:3.97|P-value:3.58E-5||SGD DESC:3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication Gene:OGG1(YML060W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OSH2(YDL019C)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEF1(YGR058W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PKR1(YMR123W)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PRS5(YOL061W)|FD-Score:4.04|P-value:2.71E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress Gene:RDR1(YOR380W)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:RPN13(YLR421C)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress Gene:RPS1A(YLR441C)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication Gene:SAM2(YDR502C)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SCO2(YBR024W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SEF1(YBL066C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Putative transcription factor, has homolog in Kluyveromyces lactis Gene:SNF2(YOR290C)|FD-Score:-3.14|P-value:8.33E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNT2(YGL131C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SPS1(YDR523C)|FD-Score:3.1|P-value:9.66E-4||SGD DESC:Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis Gene:SSM4(YIL030C)|FD-Score:3.23|P-value:6.08E-4||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:SUB1(YMR039C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Transcriptional coactivator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; protein abundance increases in response to DNA replication stress Gene:THI3(YDL080C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:THP2(YHR167W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TOM1(YDR457W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:YAL056C-A(YAL056C-A_d)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR300C(YBR300C_d)|FD-Score:-3.55|P-value:1.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YDL073W(YDL073W_p)|FD-Score:3.24|P-value:5.93E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDL162C(YDL162C_d)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR306C(YDR306C_p)|FD-Score:9.06|P-value:6.60E-20||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YET3(YDL072C)|FD-Score:4.9|P-value:4.70E-7||SGD DESC:Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress Gene:YGL118C(YGL118C_d)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR201C(YGR201C_p)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Putative protein of unknown function Gene:YKL030W(YKL030W_d)|FD-Score:4.83|P-value:6.87E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR287C(YLR287C_p)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YME2(YMR302C)|FD-Score:-3.29|P-value:5.09E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YMR193C-A(YMR193C-A_d)|FD-Score:-3.24|P-value:5.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL060W5.032.46E-71.12TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YDL143W3.914.70E-50.10CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGR099W3.817.00E-50.90TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YML015C2.910.001820.14TAF11TFIID subunit (40 kDa), involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
YMR203W2.770.002800.12TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YDL108W2.650.004000.05KIN28Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters
YGL207W2.600.004690.06SPT16Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; some mutations cause reduced nucleosome occupancy over highly transcribed regions of the yeast genome
YGL201C2.530.005660.01MCM6Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
YDR299W2.520.005820.02BFR2Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
YOR319W2.510.006100.00HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YPL142C_d2.500.006150.01YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YDL087C2.490.006330.01LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YGR048W2.480.006520.11UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YIL026C2.370.008830.05IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YJL195C_d2.320.010200.01YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR306C_p9.066.60E-20YDR306C_pF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
YDR315C5.783.68E-9IPK1Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
YDR277C5.267.14E-8MTH1Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation
YDL072C4.904.70E-7YET3Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
YKL030W_d4.836.87E-7YKL030W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1
YGL203C4.741.08E-6KEX1Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
YDR068W4.542.86E-6DOS2Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YDL019C4.493.54E-6OSH2Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
YLR441C4.493.58E-6RPS1ARibosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication
YGR058W4.251.08E-5PEF1Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
YGR201C_p4.072.32E-5YGR201C_pPutative protein of unknown function
YGL118C_d4.062.49E-5YGL118C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YGL017W4.052.52E-5ATE1Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
YBR024W4.052.54E-5SCO2Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication
YOL061W4.042.71E-5PRS55-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress

GO enrichment analysis for SGTC_1563
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2991.39E-122SGTC_15666,7-dimethoxy-4-ethylcoumarin 85.4 μMTimTec (Pure natural product library)6887610.04
0.2664.39E-96SGTC_1569carminic acid 40.6 μMTimTec (Pure natural product library)147490.0421053
0.2492.15E-84SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.197183
0.0991.94E-14SGTC_2419paclitaxel 166.5 μMMiscellaneous46660.0551181
0.0921.45E-12SGTC_14770139-0234 16.0 μMChemDiv (Drug-like library)6208800.027027
0.0863.00E-11SGTC_1121fusaric acid 630.5 nMTimTec (Natural product library)34420.0405405
0.0791.11E-9SGTC_14394092-1168 46.0 μMChemDiv (Drug-like library)28862400.060241
0.0791.58E-9SGTC_1140364-0001 490.3 μMChemDiv (Drug-like library)40036550.0609756
0.0781.70E-9SGTC_23869075165 157.4 μMChembridge (Fragment library)164522770.0666667
0.0782.22E-9SGTC_33139133968 57.0 μMChembridge (Drug-like library)14946430.0537634
0.0732.01E-8SGTC_1777st045864 34.8 μMTimTec (Natural product derivative library)46914620.0705882
0.0722.69E-8SGTC_2693st077581 40.7 μMTimTec (Natural product derivative library)252387610.0740741
0.0681.76E-7SGTC_260nsc-65238 4.0 μMMiscellaneous32462520.126582endomembrane recycling
0.0655.72E-7SGTC_12570669-0121 16.0 μMChemDiv (Drug-like library)43078550.025
0.0647.20E-7SGTC_1586deoxycorticosterone acetate 53.7 μMTimTec (Pure natural product library)59520.2125

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2650prasterone100 μM0.39344316759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.3934435881Miscellaneous288.424423.33812
SGTC_1715st03216010.23 μM0.3424665289825TimTec (Natural product derivative library)371.513022.21813
SGTC_1757st04162031.84 μM0.3333335289815TimTec (Natural product derivative library)329.476342.42223
SGTC_2074185-00104.49 μM0.3253761753ChemDiv (Drug-like library)427.66245.12413endomembrane recycling
SGTC_2607solasodine47.35 μM0.3164566710642Microsource (Natural product library)413.635824.36723
SGTC_1609st00157446.4 μM0.3037973960012TimTec (Natural product derivative library)430.576923.5615
SGTC_266formosanin C406 nM0.23364573597Miscellaneous1015.184780.311020sphingolipid biosynthesis & PDR1
SGTC_25005-alpha-androstan-3,17-dione61.44 μM0.2205886710655Microsource (Natural product library)288.424423.40202
SGTC_1586deoxycorticosterone acetate53.7 μM0.21255952TimTec (Pure natural product library)372.497783.56704