6,7-dimethoxy-4-ethylcoumarin

4-ethyl-6,7-dimethoxychromen-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1566
Screen concentration 85.4 μM
Source TimTec (Pure natural product library)
PubChem CID 688761
SMILES CCC1=CC(=O)OC2=CC(=C(C=C12)OC)OC
Standardized SMILES CCC1=CC(=O)Oc2cc(OC)c(OC)cc12
Molecular weight 234.2479
ALogP 2.77
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.72
% growth inhibition (Hom. pool) 3.61


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 688761
Download HIP data (tab-delimited text)  (excel)
Gene:CDC53(YDL132W)|FD-Score:-4.81|P-value:7.64E-7|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:DIS3(YOL021C)|FD-Score:4.37|P-value:6.26E-6|Clearance:0.56||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:PSF2(YJL072C)|FD-Score:5.28|P-value:6.64E-8|Clearance:0.91||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SEC13(YLR208W)|FD-Score:3.8|P-value:7.11E-5|Clearance:0.08||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC24(YIL109C)|FD-Score:3.72|P-value:9.93E-5|Clearance:0.59||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SEC59(YMR013C)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation Gene:SEC8(YPR055W)|FD-Score:-3.19|P-value:7.11E-4|Clearance:0||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:TIF35(YDR429C)|FD-Score:-3.73|P-value:9.47E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TSR1(YDL060W)|FD-Score:3.14|P-value:8.57E-4|Clearance:0.05||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UTR5(YEL035C_p)|FD-Score:3.81|P-value:6.94E-5|Clearance:0.01||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:CDC53(YDL132W)|FD-Score:-4.81|P-value:7.64E-7|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:DIS3(YOL021C)|FD-Score:4.37|P-value:6.26E-6|Clearance:0.56||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:PSF2(YJL072C)|FD-Score:5.28|P-value:6.64E-8|Clearance:0.91||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:SEC13(YLR208W)|FD-Score:3.8|P-value:7.11E-5|Clearance:0.08||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC24(YIL109C)|FD-Score:3.72|P-value:9.93E-5|Clearance:0.59||SGD DESC:Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p Gene:SEC59(YMR013C)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:Dolichol kinase, catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation Gene:SEC8(YPR055W)|FD-Score:-3.19|P-value:7.11E-4|Clearance:0||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:TIF35(YDR429C)|FD-Score:-3.73|P-value:9.47E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TSR1(YDL060W)|FD-Score:3.14|P-value:8.57E-4|Clearance:0.05||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UTR5(YEL035C_p)|FD-Score:3.81|P-value:6.94E-5|Clearance:0.01||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 688761
Download HOP data (tab-delimited text)  (excel)
Gene:ARR3(YPR201W)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:BNR1(YIL159W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BUG1(YDL099W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Gene:CHS7(YHR142W)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG6(YNL041C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.71|P-value:1.21E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ2(YNR041C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:CST9(YLR394W)|FD-Score:3.75|P-value:8.71E-5||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:DCC1(YCL016C)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:ERP5(YHR110W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GEP7(YGL057C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GIS1(YDR096W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication Gene:HTB2(YBL002W)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IBA57(YJR122W)|FD-Score:-3.74|P-value:9.29E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:ILM1(YJR118C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IPK1(YDR315C)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IRS4(YKR019C)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KEX1(YGL203C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KIN1(YDR122W)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:KTR3(YBR205W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress Gene:LEU1(YGL009C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway Gene:LNP1(YHR192W_p)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LOT6(YLR011W)|FD-Score:-3.22|P-value:6.30E-4||SGD DESC:FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions Gene:MAL11(YGR289C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MAM1(YER106W)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MDM38(YOL027C)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MRK1(YDL079C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MTH1(YDR277C)|FD-Score:4.82|P-value:7.30E-7||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:NFI1(YOR156C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:OSH2(YDL019C)|FD-Score:4.35|P-value:6.81E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEF1(YGR058W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PET18(YCR020C)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PKR1(YMR123W)|FD-Score:-6.8|P-value:5.40E-12||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POP2(YNR052C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RDH54(YBR073W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RML2(YEL050C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL6B(YLR448W)|FD-Score:-5|P-value:2.89E-7||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RSM19(YNR037C)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RTR1(YER139C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:-5.34|P-value:4.75E-8||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SEA4(YBL104C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SGF73(YGL066W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SMY1(YKL079W)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SNF2(YOR290C)|FD-Score:-4.57|P-value:2.49E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SYM1(YLR251W)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TAT1(YBR069C)|FD-Score:4.96|P-value:3.54E-7||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TEA1(YOR337W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:THI3(YDL080C)|FD-Score:4.4|P-value:5.38E-6||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TLG2(YOL018C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM1(YDR457W)|FD-Score:-5.43|P-value:2.85E-8||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TPN1(YGL186C)|FD-Score:6.02|P-value:8.55E-10||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPP1(YMR156C)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRI1(YMR233W)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:11.7|P-value:6.71E-32||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.26|P-value:7.40E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.76|P-value:9.67E-19||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.78|P-value:8.99E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:VPS27(YNR006W)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS36(YLR417W)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS71(YML041C)|FD-Score:5.93|P-value:1.48E-9||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR226C(YBR226C_d)|FD-Score:-3.83|P-value:6.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCR022C(YCR022C_d)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YDL162C(YDL162C_d)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR222W(YDR222W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR306C(YDR306C_p)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YDR541C(YDR541C_p)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YFR006W(YFR006W_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGL118C(YGL118C_d)|FD-Score:5|P-value:2.86E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL217C(YGL217C_d)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YGR050C(YGR050C_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR201C(YGR201C_p)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Putative protein of unknown function Gene:YIL163C(YIL163C_p)|FD-Score:-3.82|P-value:6.57E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YJU3(YKL094W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL023W(YKL023W_p)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL030W(YKL030W_d)|FD-Score:6.2|P-value:2.80E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKR047W(YKR047W_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR287C(YLR287C_p)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YML089C(YML089C_d)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YMR193C-A(YMR193C-A_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR196W(YMR196W_p)|FD-Score:-4.98|P-value:3.19E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YOR296W(YOR296W_p)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YPK9(YOR291W)|FD-Score:-3.15|P-value:8.08E-4||SGD DESC:Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome Gene:YPR127W(YPR127W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:ARR3(YPR201W)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:BNR1(YIL159W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BUG1(YDL099W)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Gene:CHS7(YHR142W)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COG6(YNL041C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.71|P-value:1.21E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ2(YNR041C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis Gene:CST9(YLR394W)|FD-Score:3.75|P-value:8.71E-5||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:DCC1(YCL016C)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Subunit of a complex with Ctf8p and Ctf18p that shares some components with Replication Factor C, required for sister chromatid cohesion and telomere length maintenance Gene:ERP5(YHR110W)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GEP7(YGL057C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GIS1(YDR096W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication Gene:HTB2(YBL002W)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation Gene:IBA57(YJR122W)|FD-Score:-3.74|P-value:9.29E-5||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:ILM1(YJR118C)|FD-Score:3.3|P-value:4.91E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IPK1(YDR315C)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:IRS4(YKR019C)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KEX1(YGL203C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:KIN1(YDR122W)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p Gene:KTR3(YBR205W)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress Gene:LEU1(YGL009C)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway Gene:LNP1(YHR192W_p)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LOT6(YLR011W)|FD-Score:-3.22|P-value:6.30E-4||SGD DESC:FMN-dependent NAD(P)H:quinone reductase, may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions Gene:MAL11(YGR289C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization Gene:MAM1(YER106W)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MDM38(YOL027C)|FD-Score:-3.46|P-value:2.74E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MRK1(YDL079C)|FD-Score:3.61|P-value:1.53E-4||SGD DESC:Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation Gene:MTH1(YDR277C)|FD-Score:4.82|P-value:7.30E-7||SGD DESC:Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation Gene:NFI1(YOR156C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:OSH2(YDL019C)|FD-Score:4.35|P-value:6.81E-6||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEF1(YGR058W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PET18(YCR020C)|FD-Score:4.95|P-value:3.63E-7||SGD DESC:Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin Gene:PKR1(YMR123W)|FD-Score:-6.8|P-value:5.40E-12||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:POP2(YNR052C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RDH54(YBR073W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RML2(YEL050C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RPL6B(YLR448W)|FD-Score:-5|P-value:2.89E-7||SGD DESC:Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication Gene:RPS16B(YDL083C)|FD-Score:3.16|P-value:7.91E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication Gene:RSM19(YNR037C)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RTR1(YER139C)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:RTS1(YOR014W)|FD-Score:-5.34|P-value:4.75E-8||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:SEA4(YBL104C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SGF73(YGL066W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:SAGA complex subunit; has a role in anchoring the deubiquitination module into SAGA and SLIK complexes; involved in preinitiation complex assembly at promoters; human ortholog ataxin-7 is associated with spinocerebellar ataxia diseases; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:SMY1(YKL079W)|FD-Score:-3.38|P-value:3.65E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SNF2(YOR290C)|FD-Score:-4.57|P-value:2.49E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SYM1(YLR251W)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TAT1(YBR069C)|FD-Score:4.96|P-value:3.54E-7||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TEA1(YOR337W)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Ty1 enhancer activator required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein Gene:THI3(YDL080C)|FD-Score:4.4|P-value:5.38E-6||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TLG2(YOL018C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM1(YDR457W)|FD-Score:-5.43|P-value:2.85E-8||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TPN1(YGL186C)|FD-Score:6.02|P-value:8.55E-10||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPP1(YMR156C)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRI1(YMR233W)|FD-Score:3.94|P-value:4.12E-5||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:11.7|P-value:6.71E-32||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.26|P-value:7.40E-8||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.76|P-value:9.67E-19||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.78|P-value:8.99E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:VPS27(YNR006W)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS36(YLR417W)|FD-Score:-3.11|P-value:9.34E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS71(YML041C)|FD-Score:5.93|P-value:1.48E-9||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR226C(YBR226C_d)|FD-Score:-3.83|P-value:6.33E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W Gene:YCR022C(YCR022C_d)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR022C is not an essential gene Gene:YDL162C(YDL162C_d)|FD-Score:3.84|P-value:6.07E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly Gene:YDR222W(YDR222W_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR306C(YDR306C_p)|FD-Score:3.83|P-value:6.36E-5||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YDR541C(YDR541C_p)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YFR006W(YFR006W_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGL118C(YGL118C_d)|FD-Score:5|P-value:2.86E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL217C(YGL217C_d)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W Gene:YGR050C(YGR050C_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR201C(YGR201C_p)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Putative protein of unknown function Gene:YIL163C(YIL163C_p)|FD-Score:-3.82|P-value:6.57E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YJU3(YKL094W)|FD-Score:3.41|P-value:3.30E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL023W(YKL023W_p)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL030W(YKL030W_d)|FD-Score:6.2|P-value:2.80E-10||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKR047W(YKR047W_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR287C(YLR287C_p)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YML089C(YML089C_d)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YMR193C-A(YMR193C-A_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR196W(YMR196W_p)|FD-Score:-4.98|P-value:3.19E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YOR296W(YOR296W_p)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YPK9(YOR291W)|FD-Score:-3.15|P-value:8.08E-4||SGD DESC:Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome Gene:YPR127W(YPR127W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL072C5.286.64E-80.91PSF2Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YOL021C4.376.26E-60.56DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YEL035C_p3.816.94E-50.01UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YLR208W3.807.11E-50.08SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YIL109C3.729.93E-50.58SEC24Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
YDL060W3.148.57E-40.05TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YGL233W3.080.001020.10SEC15Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
YGR099W2.980.001420.01TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YBR123C2.970.001480.05TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
YDR299W2.920.001740.09BFR2Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
YJL090C2.830.002310.02DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YKL004W2.820.002420.01AUR1Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
YLR153C2.810.002470.02ACS2Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
YGR115C_d2.800.002590.11YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YGL201C2.680.003660.01MCM6Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER090W11.706.71E-32TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR354W8.769.67E-19TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YKL030W_d6.202.80E-10YKL030W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1
YGL186C6.028.55E-10TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YML041C5.931.48E-9VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YKL211C5.267.40E-8TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGL118C_d5.002.86E-7YGL118C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR069C4.963.54E-7TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YCR020C4.953.63E-7PET18Protein of unknown function, has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
YDR277C4.827.30E-7MTH1Negative regulator of the glucose-sensing signal transduction pathway, required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation
YGL026C4.788.99E-7TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YGL005C4.711.21E-6COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YDR315C4.711.25E-6IPK1Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
YDL080C4.405.38E-6THI3Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis
YDL019C4.356.81E-6OSH2Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability

GO enrichment analysis for SGTC_1566
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3728.05E-193SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0615385
0.2991.39E-122SGTC_1563conessine 56.1 μMTimTec (Pure natural product library)4410820.04
0.2601.87E-91SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0816327
0.2552.50E-88SGTC_1548st061468 90.8 μMTimTec (Pure natural product library)3907990.409091
0.2449.97E-81SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.218182
0.2398.51E-78SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.173077
0.2359.52E-75SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0666667
0.2351.10E-74SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.078125
0.2294.27E-71SGTC_1114scopoletin 1.7 μMTimTec (Natural product library)52804600.355556
0.2263.51E-69SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.0615385
0.2252.20E-68SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.175439
0.2231.59E-67SGTC_1104tacrolimus 29.8 μMNIH Clinical Collection235817960.079646
0.2231.66E-67SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.290909
0.2213.64E-66SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.047619
0.2201.48E-65SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.037037

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1518st03856790.8 μM0.63888977966TimTec (Pure natural product library)220.221282.31304
SGTC_7234182-0001115 μM0.547619744821ChemDiv (Drug-like library)218.248463.27203
SGTC_1912st06020990.8 μM0.434783688787TimTec (Natural product derivative library)220.221281.9204
SGTC_15447-methoxy-4-methylcoumarin105 μM0.418605390807TimTec (Pure natural product library)190.19532.32903
SGTC_1548st06146890.8 μM0.409091390799TimTec (Pure natural product library)220.221282.31304
SGTC_1903st06021178.3 μM0.404255688960TimTec (Natural product derivative library)220.221281.9204
SGTC_1526st05151875 μM0.4673993TimTec (Pure natural product library)266.677043.44504
SGTC_1902st06021270.5 μM0.391304688944TimTec (Natural product derivative library)204.221882.42203
SGTC_1904st06022578 μM0.39130466595TimTec (Natural product derivative library)204.221882.67803
SGTC_1571st07008073.4 μM0.3818184315245TimTec (Pure natural product library)272.299141.89505