st069303

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1567
Screen concentration 68.4 μM
Source TimTec (Pure natural product library)
PubChem CID 5581319
SMILES CC=C1CN2CCC34C2CC1C(=C3NC5=CC=CC=C45)C=O
Standardized SMILES CC=C1CN2CCC34C2CC1C(=C3Nc5ccccc45)C=O
Molecular weight 292.3749
ALogP 2.25
H-bond donor count 1
H-bond acceptor count 3
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.82
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5581319
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:4.03|P-value:2.83E-5|Clearance:0.16||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:DED1(YOR204W)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.05||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:EXO84(YBR102C)|FD-Score:4.42|P-value:4.99E-6|Clearance:0.06||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:MTR3(YGR158C)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NIP7(YPL211W)|FD-Score:3.87|P-value:5.43E-5|Clearance:0.16||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:PKC1(YBL105C)|FD-Score:5.42|P-value:3.00E-8|Clearance:0.41||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PMA1(YGL008C)|FD-Score:-3.36|P-value:3.85E-4|Clearance:0||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:RPB8(YOR224C)|FD-Score:4.09|P-value:2.13E-5|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPF2(YKR081C)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL33A(YPL143W)|FD-Score:3.24|P-value:5.97E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RRP4(YHR069C)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC58(YLR033W)|FD-Score:8.92|P-value:2.25E-19|Clearance:2.19||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:4.86|P-value:5.95E-7|Clearance:0.14||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC18(YBR080C)|FD-Score:6.54|P-value:3.07E-11|Clearance:0.99||SGD DESC:ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF Gene:SEC8(YPR055W)|FD-Score:6.73|P-value:8.39E-12|Clearance:0.19||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SLD3(YGL113W)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.01||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SLU7(YDR088C)|FD-Score:3.1|P-value:9.72E-4|Clearance:0.02||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:SNP1(YIL061C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.25||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:TAF3(YPL011C)|FD-Score:5.55|P-value:1.41E-8|Clearance:0.13||SGD DESC:TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation Gene:TAF7(YMR227C)|FD-Score:5.01|P-value:2.79E-7|Clearance:0.15||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TFB2(YPL122C)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.09||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:THG1(YGR024C)|FD-Score:-3.2|P-value:6.82E-4|Clearance:0||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TOM40(YMR203W)|FD-Score:4.36|P-value:6.45E-6|Clearance:0.27||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP11(YKL099C)|FD-Score:-3.32|P-value:4.47E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTR5(YEL035C_p)|FD-Score:4.72|P-value:1.18E-6|Clearance:0.3||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YGR265W(YGR265W_d)|FD-Score:3.4|P-value:3.34E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase Gene:ARC40(YBR234C)|FD-Score:4.03|P-value:2.83E-5|Clearance:0.16||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:DED1(YOR204W)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.05||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:EXO84(YBR102C)|FD-Score:4.42|P-value:4.99E-6|Clearance:0.06||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:MTR3(YGR158C)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NIP7(YPL211W)|FD-Score:3.87|P-value:5.43E-5|Clearance:0.16||SGD DESC:Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p Gene:PKC1(YBL105C)|FD-Score:5.42|P-value:3.00E-8|Clearance:0.41||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PMA1(YGL008C)|FD-Score:-3.36|P-value:3.85E-4|Clearance:0||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:RPB8(YOR224C)|FD-Score:4.09|P-value:2.13E-5|Clearance:0.07||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPF2(YKR081C)|FD-Score:-3.62|P-value:1.48E-4|Clearance:0||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPL33A(YPL143W)|FD-Score:3.24|P-value:5.97E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RRP4(YHR069C)|FD-Score:3.71|P-value:1.05E-4|Clearance:0.05||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC58(YLR033W)|FD-Score:8.92|P-value:2.25E-19|Clearance:2.19||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:4.86|P-value:5.95E-7|Clearance:0.14||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC18(YBR080C)|FD-Score:6.54|P-value:3.07E-11|Clearance:0.99||SGD DESC:ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF Gene:SEC8(YPR055W)|FD-Score:6.73|P-value:8.39E-12|Clearance:0.19||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SLD3(YGL113W)|FD-Score:3.11|P-value:9.29E-4|Clearance:0.01||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SLU7(YDR088C)|FD-Score:3.1|P-value:9.72E-4|Clearance:0.02||SGD DESC:RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain Gene:SNP1(YIL061C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.25||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:TAF3(YPL011C)|FD-Score:5.55|P-value:1.41E-8|Clearance:0.13||SGD DESC:TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation Gene:TAF7(YMR227C)|FD-Score:5.01|P-value:2.79E-7|Clearance:0.15||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TFB2(YPL122C)|FD-Score:3.36|P-value:3.92E-4|Clearance:0.09||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:THG1(YGR024C)|FD-Score:-3.2|P-value:6.82E-4|Clearance:0||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TOM40(YMR203W)|FD-Score:4.36|P-value:6.45E-6|Clearance:0.27||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:UTP11(YKL099C)|FD-Score:-3.32|P-value:4.47E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTR5(YEL035C_p)|FD-Score:4.72|P-value:1.18E-6|Clearance:0.3||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YGR265W(YGR265W_d)|FD-Score:3.4|P-value:3.34E-4|Clearance:0.04||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5581319
Download HOP data (tab-delimited text)  (excel)
Gene:ADR1(YDR216W)|FD-Score:-3.91|P-value:4.61E-5||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AIP1(YMR092C)|FD-Score:6.21|P-value:2.60E-10||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ATP10(YLR393W)|FD-Score:-3.85|P-value:6.02E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BCK1(YJL095W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:DUG2(YBR281C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ECM11(YDR446W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure Gene:FET4(YMR319C)|FD-Score:4.38|P-value:6.01E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FYV12(YOR183W_p)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:HAM1(YJR069C)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HAP4(YKL109W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HCH1(YNL281W)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HFA1(YMR207C)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ICS3(YJL077C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IRC21(YMR073C)|FD-Score:5.42|P-value:2.92E-8||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KNS1(YLL019C)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LAG1(YHL003C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LEE1(YPL054W)|FD-Score:4.37|P-value:6.25E-6||SGD DESC:Zinc-finger protein of unknown function Gene:LTE1(YAL024C)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MDM38(YOL027C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MLP2(YIL149C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MPD1(YOR288C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MRPL51(YPR100W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH1(YHR120W)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSY1(YPL097W)|FD-Score:-3.79|P-value:7.45E-5||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MUP3(YHL036W)|FD-Score:4|P-value:3.14E-5||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NAM8(YHR086W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NUC1(YJL208C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:NVJ1(YHR195W)|FD-Score:8.73|P-value:1.26E-18||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OXA1(YER154W)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:OYE2(YHR179W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PBP1(YGR178C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PEP4(YPL154C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PET111(YMR257C)|FD-Score:-4.02|P-value:2.91E-5||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PEX19(YDL065C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PEX28(YHR150W)|FD-Score:-3.92|P-value:4.36E-5||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PIN3(YPR154W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:QDR3(YBR043C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RCF1(YML030W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RIM1(YCR028C-A)|FD-Score:-4.63|P-value:1.80E-6||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPS29B(YDL061C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:RRF1(YHR038W)|FD-Score:-4.93|P-value:4.07E-7||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:SAC3(YDR159W)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAS5(YOR213C)|FD-Score:-3.87|P-value:5.41E-5||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SHE1(YBL031W)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SHH3(YMR118C_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SLM3(YDL033C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLS1(YLR139C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SLZ1(YNL196C_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF3(YDL194W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SPT4(YGR063C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SSK1(YLR006C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:TRP1(YDR007W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TYW3(YGL050W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:VPS1(YKR001C)|FD-Score:-4.35|P-value:6.79E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDR149C(YDR149C_d)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR541C(YDR541C_p)|FD-Score:7.33|P-value:1.17E-13||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER046W-A(YER046W-A_d)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER079W(YER079W_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function Gene:YER087C-A(YER087C-A_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YGL176C(YGL176C_p)|FD-Score:-6.35|P-value:1.11E-10||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YIL102C(YIL102C_p)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Putative protein of unknown function Gene:YJL064W(YJL064W_d)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YJR111C(YJR111C_p)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YKR075C(YKR075C_p)|FD-Score:4.44|P-value:4.40E-6||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR296W(YLR296W_d)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR034C(YMR034C_p)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR085W(YMR085W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR245W(YMR245W_d)|FD-Score:4.27|P-value:9.65E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR338W(YOR338W_p)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YPL035C(YPL035C_d)|FD-Score:4.27|P-value:9.88E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Gene:YPL197C(YPL197C_d)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YPR146C(YPR146C_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZRT3(YKL175W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency Gene:ADR1(YDR216W)|FD-Score:-3.91|P-value:4.61E-5||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AIP1(YMR092C)|FD-Score:6.21|P-value:2.60E-10||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ATP10(YLR393W)|FD-Score:-3.85|P-value:6.02E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the F0 sector of mitochondrial F1F0 ATP synthase, interacts genetically with ATP6 Gene:BCK1(YJL095W)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:DUG2(YBR281C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:ECM11(YDR446W)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure Gene:FET4(YMR319C)|FD-Score:4.38|P-value:6.01E-6||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FYV12(YOR183W_p)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:-4.16|P-value:1.56E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:HAM1(YJR069C)|FD-Score:3.86|P-value:5.67E-5||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:HAP4(YKL109W)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HCH1(YNL281W)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HFA1(YMR207C)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:Mitochondrial acetyl-coenzyme A carboxylase; catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ICS3(YJL077C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IRC21(YMR073C)|FD-Score:5.42|P-value:2.92E-8||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:KNS1(YLL019C)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LAG1(YHL003C)|FD-Score:3.33|P-value:4.32E-4||SGD DESC:Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress Gene:LEE1(YPL054W)|FD-Score:4.37|P-value:6.25E-6||SGD DESC:Zinc-finger protein of unknown function Gene:LTE1(YAL024C)|FD-Score:3.95|P-value:3.91E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MDM38(YOL027C)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MLP2(YIL149C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MPD1(YOR288C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation Gene:MRPL51(YPR100W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSH1(YHR120W)|FD-Score:-3.18|P-value:7.37E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSY1(YPL097W)|FD-Score:-3.79|P-value:7.45E-5||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:MUP3(YHL036W)|FD-Score:4|P-value:3.14E-5||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NAM8(YHR086W)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:NUC1(YJL208C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:NVJ1(YHR195W)|FD-Score:8.73|P-value:1.26E-18||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OXA1(YER154W)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Mitochondrial inner membrane insertase, mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane, interacts with mitochondrial ribosomes; conserved from bacteria to animals Gene:OYE2(YHR179W)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PBP1(YGR178C)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PEP4(YPL154C)|FD-Score:3.12|P-value:8.94E-4||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PET111(YMR257C)|FD-Score:-4.02|P-value:2.91E-5||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PEX19(YDL065C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning Gene:PEX28(YHR150W)|FD-Score:-3.92|P-value:4.36E-5||SGD DESC:Peroxisomal integral membrane peroxin, involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p Gene:PIN3(YPR154W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced Gene:QDR3(YBR043C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin Gene:RCF1(YML030W)|FD-Score:4.18|P-value:1.46E-5||SGD DESC:Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast Gene:RIM1(YCR028C-A)|FD-Score:-4.63|P-value:1.80E-6||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RPS29B(YDL061C)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:RRF1(YHR038W)|FD-Score:-4.93|P-value:4.07E-7||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:SAC3(YDR159W)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAS5(YOR213C)|FD-Score:-3.87|P-value:5.41E-5||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SHE1(YBL031W)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SHH3(YMR118C_p)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase Gene:SLM3(YDL033C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SLS1(YLR139C)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SLZ1(YNL196C_p)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SNF3(YDL194W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication Gene:SPT4(YGR063C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SSK1(YLR006C)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:TRP1(YDR007W)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TYW3(YGL050W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:VPS1(YKR001C)|FD-Score:-4.35|P-value:6.79E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS61(YDR136C_d)|FD-Score:-4.01|P-value:3.07E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDR149C(YDR149C_d)|FD-Score:4.67|P-value:1.49E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR541C(YDR541C_p)|FD-Score:7.33|P-value:1.17E-13||SGD DESC:Putative dihydrokaempferol 4-reductase Gene:YER046W-A(YER046W-A_d)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER079W(YER079W_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function Gene:YER087C-A(YER087C-A_d)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YGL176C(YGL176C_p)|FD-Score:-6.35|P-value:1.11E-10||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YIL102C(YIL102C_p)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Putative protein of unknown function Gene:YJL064W(YJL064W_d)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YJR111C(YJR111C_p)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondria Gene:YKR075C(YKR075C_p)|FD-Score:4.44|P-value:4.40E-6||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YLR296W(YLR296W_d)|FD-Score:-3.35|P-value:4.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR034C(YMR034C_p)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR085W(YMR085W_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR245W(YMR245W_d)|FD-Score:4.27|P-value:9.65E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR338W(YOR338W_p)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YPL035C(YPL035C_d)|FD-Score:4.27|P-value:9.88E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene Gene:YPL197C(YPL197C_d)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YPR146C(YPR146C_d)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZRT3(YKL175W)|FD-Score:3.11|P-value:9.48E-4||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W8.922.25E-192.19RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YPR055W6.738.39E-120.19SEC8Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
YBR080C6.543.07E-110.99SEC18ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF
YPL011C5.551.41E-80.13TAF3TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
YBL105C5.423.00E-80.41PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YMR227C5.012.79E-70.15TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YCR052W4.865.95E-70.14RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YEL035C_p4.721.18E-60.30UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YBR102C4.424.99E-60.06EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YMR203W4.366.45E-60.27TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YOR224C4.092.13E-50.07RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YBR234C4.032.83E-50.16ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YPL211W3.875.43E-50.16NIP7Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
YHR069C3.711.05E-40.05RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YIL061C3.651.29E-40.25SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR195W8.731.26E-18NVJ1Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)
YDR541C_p7.331.17E-13YDR541C_pPutative dihydrokaempferol 4-reductase
YMR092C6.212.60E-10AIP1Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress
YMR073C5.422.92E-8IRC21Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
YDR149C_d4.671.49E-6YDR149C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth
YBL031W4.651.65E-6SHE1Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
YNL281W4.582.32E-6HCH1Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
YKR075C_p4.444.40E-6YKR075C_pProtein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
YMR319C4.386.01E-6FET4Low-affinity Fe(II) transporter of the plasma membrane
YPL054W4.376.25E-6LEE1Zinc-finger protein of unknown function
YMR245W_d4.279.65E-6YMR245W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPL035C_d4.279.88E-6YPL035C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene
YLR006C4.261.01E-5SSK1Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate
YBR043C4.231.19E-5QDR3Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin
YKL109W4.201.35E-5HAP4Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex

GO enrichment analysis for SGTC_1567
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1102.88E-17SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.084507heme biosynthesis & mitochondrial translocase
0.1071.81E-16SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.111111RSC complex & mRNA processing
0.1054.53E-16SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.0923077
0.1055.52E-16SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0595238RSC & ERG11
0.1048.29E-16SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.0833333heme biosynthesis & mitochondrial translocase
0.1001.45E-14SGTC_1992st073643 23.3 μMTimTec (Natural product derivative library)31612920.0454545mitochondrial processes
0.0993.03E-14SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0864198RSC complex & mRNA processing
0.0937.64E-13SGTC_1831st055328 12.0 μMTimTec (Natural product derivative library)11030680.121622heme biosynthesis & mitochondrial translocase
0.0872.51E-11SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.1RSC complex & mRNA processing
0.0855.24E-11SGTC_7830848-0141 92.7 μMChemDiv (Drug-like library)67550760.0465116RSC & ERG11
0.0849.95E-11SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.0843373RSC complex & mRNA processing
0.0822.32E-10SGTC_14780453-0656 35.7 μMChemDiv (Drug-like library)52584960.0617284
0.0814.15E-10SGTC_21695-(ethoxymethyl)quinolin-8-ol 49.5 μMChembridge (Fragment library)2796210.0649351
0.0814.86E-10SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.121951heme biosynthesis & mitochondrial translocase
0.0806.01E-10SGTC_1583clindamycin 39.6 μMTimTec (Pure natural product library)326030.0566038mitochondrial processes

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22226885208172.14 μM0.25609514Chembridge (Fragment library)233.26311.43313
SGTC_1635st00703542.4 μM0.1951223716403TimTec (Natural product derivative library)351.31622-0.25226
SGTC_25303-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene92.62 μM0.1884066708760Microsource (Natural product library)216.31873.35611
SGTC_235890371574 μM0.1857142388925Chembridge (Fragment library)231.250520.20513
SGTC_2706st07823982.5 μM0.18571415434492TimTec (Natural product derivative library)242.273161.08712
SGTC_2724maprotiline69.64 μM0.1857144011Miscellaneous277.403324.11611ERG2
SGTC_23799035213200 μM0.180556646551Chembridge (Fragment library)240.300342.32213
SGTC_2007401153971.82 μM0.17391345595301Chembridge (Fragment library)285.180181.42712cell wall signaling
SGTC_2989907364971.43 μM0.1728421367202Chembridge (Drug-like library)352.426863.34613
SGTC_1520flindersine88 μM0.16901468230TimTec (Pure natural product library)227.258521.70412