verbenone

2,6,6-trimethylbicyclo[3.1.1]hept-2-en-4-one

A natural terpanoid with antifungal properties.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1570
Screen concentration 133.0 μM
Source TimTec (Pure natural product library)
PubChem CID 29025
SMILES CC1=CC(=O)C2CC1C2(C)C
Standardized SMILES CC1=CC(=O)C2CC1C2(C)C
Molecular weight 150.2176
ALogP 2.08
H-bond donor count 0
H-bond acceptor count 1
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.35
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 29025
Download HIP data (tab-delimited text)  (excel)
Gene:DBP9(YLR276C)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.12||SGD DESC:DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain Gene:JIP5(YPR169W)|FD-Score:4.79|P-value:8.55E-7|Clearance:0.5||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MRPS18(YNL306W)|FD-Score:3.77|P-value:8.04E-5|Clearance:0.14||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:RLP24(YLR009W)|FD-Score:3.18|P-value:7.41E-4|Clearance:0.12||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB11(YOL005C)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPO26(YPR187W)|FD-Score:3.31|P-value:4.58E-4|Clearance:0.14||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RVB1(YDR190C)|FD-Score:-4.29|P-value:8.96E-6|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC39(YLR440C)|FD-Score:4.59|P-value:2.19E-6|Clearance:0.5||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SLD2(YKL108W)|FD-Score:4.1|P-value:2.11E-5|Clearance:0.2||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:SMP3(YOR149C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.32||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:DBP9(YLR276C)|FD-Score:3.89|P-value:4.98E-5|Clearance:0.12||SGD DESC:DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain Gene:JIP5(YPR169W)|FD-Score:4.79|P-value:8.55E-7|Clearance:0.5||SGD DESC:Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein Gene:MRPS18(YNL306W)|FD-Score:3.77|P-value:8.04E-5|Clearance:0.14||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:RLP24(YLR009W)|FD-Score:3.18|P-value:7.41E-4|Clearance:0.12||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPB11(YOL005C)|FD-Score:-3.37|P-value:3.78E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit Gene:RPO26(YPR187W)|FD-Score:3.31|P-value:4.58E-4|Clearance:0.14||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RVB1(YDR190C)|FD-Score:-4.29|P-value:8.96E-6|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC39(YLR440C)|FD-Score:4.59|P-value:2.19E-6|Clearance:0.5||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SLD2(YKL108W)|FD-Score:4.1|P-value:2.11E-5|Clearance:0.2||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:SMP3(YOR149C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.32||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 29025
Download HOP data (tab-delimited text)  (excel)
Gene:ADE8(YDR408C)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ADR1(YDR216W)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ARL1(YBR164C)|FD-Score:4.78|P-value:8.80E-7||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASR1(YPR093C)|FD-Score:4.5|P-value:3.42E-6||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG3(YNR007C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:AXL1(YPR122W)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BTS1(YPL069C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUR2(YLR226W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II Gene:COG6(YNL041C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX5B(YIL111W)|FD-Score:4.75|P-value:1.04E-6||SGD DESC:Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication Gene:CSF1(YLR087C)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DDI1(YER143W)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DDP1(YOR163W)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress Gene:DGR1(YNL130C-A_p)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:EFM2(YBR271W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:ERV14(YGL054C)|FD-Score:-4.31|P-value:8.02E-6||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAA3(YIL009W)|FD-Score:3.75|P-value:9.00E-5||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FKH2(YNL068C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FRT2(YAL028W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:GDE1(YPL110C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GFD1(YMR255W)|FD-Score:6.33|P-value:1.21E-10||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GIP1(YBR045C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GLO1(YML004C)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:HEK2(YBL032W)|FD-Score:3.77|P-value:8.03E-5||SGD DESC:RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K Gene:HER2(YMR293C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:JJJ3(YJR097W)|FD-Score:-5.73|P-value:5.09E-9||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KCS1(YDR017C)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:MFT1(YML062C)|FD-Score:-3.92|P-value:4.51E-5||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MOT3(YMR070W)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MSN5(YDR335W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MSS11(YMR164C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:NUM1(YDR150W)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OPI10(YOL032W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX12(YMR026C)|FD-Score:4.45|P-value:4.22E-6||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PRO2(YOR323C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSK2(YOL045W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:RPB9(YGL070C)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPL19B(YBL027W)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPS27B(YHR021C)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:RRT13(YER066W_p)|FD-Score:4.46|P-value:4.06E-6||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:SAE3(YHR079C-A)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:SAS5(YOR213C)|FD-Score:-4.62|P-value:1.89E-6||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SDH4(YDR178W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SFA1(YDL168W)|FD-Score:-4.61|P-value:2.01E-6||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SPR3(YGR059W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SUV3(YPL029W)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TAT1(YBR069C)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA3(YHR009C_p)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:UBP15(YMR304W)|FD-Score:-6.3|P-value:1.52E-10||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:UBP5(YER144C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VAC7(YNL054W)|FD-Score:-4.99|P-value:2.99E-7||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VIK1(YPL253C)|FD-Score:6.29|P-value:1.56E-10||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:YCL049C(YCL049C_p)|FD-Score:6.68|P-value:1.18E-11||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YER186C(YER186C_p)|FD-Score:-5.32|P-value:5.24E-8||SGD DESC:Putative protein of unknown function Gene:YFR018C(YFR018C_p)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Putative protein of unknown function Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YHR086W-A(YHR086W-A_p)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL160C(YJL160C_p)|FD-Score:-5.69|P-value:6.53E-9||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJR015W(YJR015W_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YML096W(YML096W_p)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR103C(YMR103C_d)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR130W(YMR130W_p)|FD-Score:5.5|P-value:1.87E-8||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR195C(YPR195C_d)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:ADE8(YDR408C)|FD-Score:-3.94|P-value:4.06E-5||SGD DESC:Phosphoribosyl-glycinamide transformylase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ADR1(YDR216W)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ARL1(YBR164C)|FD-Score:4.78|P-value:8.80E-7||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ASR1(YPR093C)|FD-Score:4.5|P-value:3.42E-6||SGD DESC:Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein Gene:ATG3(YNR007C)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:AXL1(YPR122W)|FD-Score:3.12|P-value:9.11E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BTS1(YPL069C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUR2(YLR226W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II Gene:COG6(YNL041C)|FD-Score:3.28|P-value:5.13E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX5B(YIL111W)|FD-Score:4.75|P-value:1.04E-6||SGD DESC:Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication Gene:CSF1(YLR087C)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DDI1(YER143W)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:DNA damage-inducible v-SNARE binding protein with a role in suppression of protein secretion; may play a role in S-phase checkpoint control; has ubiquitin-associated (UBA), ubiquitin-like (UBL), and retroviral-like proteinase (RVP) domains Gene:DDP1(YOR163W)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress Gene:DGR1(YNL130C-A_p)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:EFM2(YBR271W)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:ERV14(YGL054C)|FD-Score:-4.31|P-value:8.02E-6||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FAA3(YIL009W)|FD-Score:3.75|P-value:9.00E-5||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:FKH2(YNL068C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Forkhead family transcription factor; plays a major role in the expression of G2/M phase genes; positively regulates transcriptional elongation; negative role in chromatin silencing at HML and HMR; substrate of the Cdc28p/Clb5p kinase; FKH2 has a paralog, FKH1, that arose from the whole genome duplication Gene:FRT2(YAL028W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:GDE1(YPL110C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes Gene:GFD1(YMR255W)|FD-Score:6.33|P-value:1.21E-10||SGD DESC:Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress Gene:GIP1(YBR045C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Meiosis-specific regulatory subunit of the Glc7p protein phosphatase, regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p Gene:GLO1(YML004C)|FD-Score:-3.3|P-value:4.87E-4||SGD DESC:Monomeric glyoxalase I, catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; expression regulated by methylglyoxal levels and osmotic stress Gene:HEK2(YBL032W)|FD-Score:3.77|P-value:8.03E-5||SGD DESC:RNA binding protein involved in the asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K Gene:HER2(YMR293C)|FD-Score:-3.5|P-value:2.30E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:JJJ3(YJR097W)|FD-Score:-5.73|P-value:5.09E-9||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KCS1(YDR017C)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:MFT1(YML062C)|FD-Score:-3.92|P-value:4.51E-5||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MOT3(YMR070W)|FD-Score:-3.21|P-value:6.62E-4||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MSN5(YDR335W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm Gene:MSS11(YMR164C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals Gene:NUM1(YDR150W)|FD-Score:3.85|P-value:5.92E-5||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OPI10(YOL032W)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:PDR1(YGL013C)|FD-Score:3.54|P-value:2.04E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX12(YMR026C)|FD-Score:4.45|P-value:4.22E-6||SGD DESC:C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder Gene:PRO2(YOR323C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PSK2(YOL045W)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication Gene:RPB9(YGL070C)|FD-Score:-3.18|P-value:7.26E-4||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPL19B(YBL027W)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPS27B(YHR021C)|FD-Score:4.64|P-value:1.72E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication Gene:RRT13(YER066W_p)|FD-Score:4.46|P-value:4.06E-6||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription Gene:SAE3(YHR079C-A)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p Gene:SAS5(YOR213C)|FD-Score:-4.62|P-value:1.89E-6||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SDH4(YDR178W)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SFA1(YDL168W)|FD-Score:-4.61|P-value:2.01E-6||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SPR3(YGR059W)|FD-Score:3.62|P-value:1.46E-4||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SUV3(YPL029W)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TAT1(YBR069C)|FD-Score:-3.35|P-value:4.08E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA3(YHR009C_p)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:UBP15(YMR304W)|FD-Score:-6.3|P-value:1.52E-10||SGD DESC:Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p Gene:UBP5(YER144C)|FD-Score:-3.2|P-value:6.85E-4||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:VAC7(YNL054W)|FD-Score:-4.99|P-value:2.99E-7||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VIK1(YPL253C)|FD-Score:6.29|P-value:1.56E-10||SGD DESC:Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p Gene:YCL049C(YCL049C_p)|FD-Score:6.68|P-value:1.18E-11||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YER186C(YER186C_p)|FD-Score:-5.32|P-value:5.24E-8||SGD DESC:Putative protein of unknown function Gene:YFR018C(YFR018C_p)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Putative protein of unknown function Gene:YGL006W-A(YGL006W-A_p)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YHR086W-A(YHR086W-A_p)|FD-Score:-3.17|P-value:7.73E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL160C(YJL160C_p)|FD-Score:-5.69|P-value:6.53E-9||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YJR015W(YJR015W_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; localizes to the endoplasmic reticulum and cytoplasm; predicted to encode a membrane transporter based on phylogenetic analysis; YJR015W is a non-essential gene Gene:YML096W(YML096W_p)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR103C(YMR103C_d)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR130W(YMR130W_p)|FD-Score:5.5|P-value:1.87E-8||SGD DESC:Putative protein of unknown function; YMR130W is not an essential gene Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR195C(YPR195C_d)|FD-Score:3.77|P-value:8.09E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR169W4.798.55E-70.50JIP5Essential protein required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein
YLR440C4.592.19E-60.50SEC39Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
YKL108W4.102.11E-50.20SLD2Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
YLR276C3.894.98E-50.12DBP9DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
YNL306W3.778.04E-50.14MRPS18Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
YOR149C3.641.38E-40.32SMP3Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
YPR187W3.314.58E-40.14RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YLR009W3.187.41E-40.12RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YIL091C3.050.001130.07UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YOR046C2.980.001420.07DBP5Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p
YNL151C2.910.001799.57E-4RPC31RNA polymerase III subunit C31
YPL043W2.910.001790.07NOP4Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
YLL018C2.840.002230.05DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YBR091C2.790.002634.74E-4TIM12Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
YBR080C2.790.002630.00SEC18ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YCL049C_p6.681.18E-11YCL049C_pProtein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene
YMR255W6.331.21E-10GFD1Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress
YPL253C6.291.56E-10VIK1Protein that forms a complex with Kar3p at the spindle pole body, possible regulator of Kar3p function in microtubule-mediated processes; required for sister chromatid cohesion; has similarity to Cik1p
YMR130W_p5.501.87E-8YMR130W_pPutative protein of unknown function; YMR130W is not an essential gene
YBR164C4.788.80E-7ARL1Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
YIL111W4.751.04E-6COX5BSubunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication
YHR021C4.641.72E-6RPS27BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication
YPR093C4.503.42E-6ASR1Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; contains a Ring/PHD finger domain similar to the mammalian rA9 protein
YER066W_p4.464.06E-6RRT13_pPutative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
YMR026C4.454.22E-6PEX12C3HC4-type RING-finger peroxin and E3 ubiquitin ligase, required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
YPL110C4.261.04E-5GDE1Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes
YOL045W4.211.26E-5PSK2PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication
YNL130C-A_p4.191.42E-5DGR1_pProtein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose
YDR178W4.042.70E-5SDH4Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain
YOR163W4.032.78E-5DDP1Polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1570
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0921.49E-12SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.0526316PDR1
0.0806.16E-10SGTC_1581himbacine 57.9 μMTimTec (Pure natural product library)52818250.112903Golgi
0.0791.47E-9SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.107143PDR1
0.0723.32E-8SGTC_28439000894 45.5 μMChembridge (Drug-like library)22420700.0862069
0.0699.39E-8SGTC_7710250-0039 27.3 μMChemDiv (Drug-like library)30051150.0535714
0.0699.40E-8SGTC_28869058944 52.0 μMChembridge (Drug-like library)49499290.0925926
0.0663.37E-7SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.142857PDR1
0.0631.52E-6SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0909091PDR1
0.0612.48E-6SGTC_33259138760 55.2 μMChembridge (Drug-like library)438449590.0735294Golgi
0.0603.37E-6SGTC_489paxilline 115.0 μMICCB bioactive library1050080.150685PDR1
0.0595.67E-6SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.0714286PDR1
0.0589.36E-6SGTC_23639053361 176.0 μMChembridge (Fragment library)64697970.0784314Golgi
0.0552.00E-5SGTC_1576erythromycin ethylsuccinate 23.2 μMTimTec (Pure natural product library)32560.0882353
0.0542.79E-5SGTC_31059121040 49.5 μMChembridge (Drug-like library)414489070.0689655
0.0543.66E-5SGTC_291SEC14.278 35.5 μMChemDiv (Drug-like library)10680440.0757576Golgi

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2415hr heat shock (37°C) + cantharidin155.45 μM0.2222222545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_2489cantharidin73.42 μM0.2222222545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_408cantharidin100 μM0.2222222545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_422cantharidin155 μM0.2222222545ICCB bioactive library196.199880.71704cell wall signaling
SGTC_1772st04843982.2 μM0.2083332927068TimTec (Natural product derivative library)243.300982.72702
SGTC_2545solidagenone89.61 μM0.2068976708572Microsource (Natural product library)316.434524.0113
SGTC_255413-methyl-4,4-bisnor-8,11,13-podocarpatrien-3-one66.67 μM0.2040826708561Microsource (Natural product library)228.32943.65701
SGTC_259usnic acid108.93 μM0.1964295646Miscellaneous344.315441.08427
SGTC_2690usnic acid100 μM0.1964295646Miscellaneous344.315441.08427
SGTC_2541muurolladie-3-one83.07 μM0.1956523989419Microsource (Natural product library)218.334583.74301excess fatty acid
SGTC_2608thymoquinone42.66 μM0.19444410281Microsource (Natural product library)164.201082.28802
SGTC_1575cyproterone acetate48 μM0.1904769880Miscellaneous416.937663.31404
SGTC_2681menadione3.18 μM0.1891894055Miscellaneous172.180022.20402superoxide