st070080

3-[(7-methoxy-2-oxochromen-4-yl)methyl-methylamino]propanenitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1571
Screen concentration 73.4 μM
Source TimTec (Pure natural product library)
PubChem CID 4315245
SMILES CN(CCC#N)CC1=CC(=O)OC2=C1C=CC(=C2)OC
Standardized SMILES COc1ccc2C(=CC(=O)Oc2c1)CN(C)CCC#N
Molecular weight 272.2991
ALogP 1.9
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.36
% growth inhibition (Hom. pool) -1.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4315245
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:-3.61|P-value:1.52E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:ARC35(YNR035C)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.33||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:BRX1(YOL077C)|FD-Score:4.19|P-value:1.42E-5|Clearance:0.38||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:MPS3(YJL019W)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.06||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:PRE8(YML092C)|FD-Score:-3.14|P-value:8.47E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RPS2(YGL123W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.21||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:TFC8(YPL007C)|FD-Score:3.34|P-value:4.23E-4|Clearance:0.06||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:UGP1(YKL035W)|FD-Score:3.8|P-value:7.20E-5|Clearance:0.19||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.11|P-value:9.30E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:APC5(YOR249C)|FD-Score:-3.61|P-value:1.52E-4|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:ARC35(YNR035C)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.33||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:BRX1(YOL077C)|FD-Score:4.19|P-value:1.42E-5|Clearance:0.38||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:MPS3(YJL019W)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.06||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:PRE8(YML092C)|FD-Score:-3.14|P-value:8.47E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RPS2(YGL123W)|FD-Score:3.61|P-value:1.55E-4|Clearance:0.21||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:TFC8(YPL007C)|FD-Score:3.34|P-value:4.23E-4|Clearance:0.06||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:UGP1(YKL035W)|FD-Score:3.8|P-value:7.20E-5|Clearance:0.19||SGD DESC:UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication Gene:YAL034C-B(YAL034C-B_d)|FD-Score:-3.11|P-value:9.30E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4315245
Download HOP data (tab-delimited text)  (excel)
Gene:ACF4(YJR083C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ADR1(YDR216W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AEP1(YMR064W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIP1(YMR092C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ALG6(YOR002W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APS1(YLR170C)|FD-Score:4|P-value:3.14E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARG4(YHR018C)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:BNA4(YBL098W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:BNI1(YNL271C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BUD16(YEL029C)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CSF1(YLR087C)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DBF2(YGR092W)|FD-Score:4.27|P-value:9.83E-6||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DBF20(YPR111W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs Gene:DBP7(YKR024C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DDR2(YOL052C-A)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:EAF6(YJR082C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:GEA2(YEL022W)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HEL1(YKR017C_p)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HNM1(YGL077C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol Gene:ICE2(YIL090W)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IFM1(YOL023W)|FD-Score:5.49|P-value:2.02E-8||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IPK1(YDR315C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:LCB4(YOR171C)|FD-Score:4.4|P-value:5.35E-6||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MET17(YLR303W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MF(ALPHA)1(YPL187W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor Gene:NCR1(YPL006W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:OAF3(YKR064W)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:PDE1(YGL248W)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation Gene:PDR3(YBL005W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PEX11(YOL147C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PFK26(YIL107C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PRY1(YJL079C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:REG2(YBR050C)|FD-Score:3.82|P-value:6.64E-5||SGD DESC:Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease Gene:RPL24B(YGR148C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPS29A(YLR388W)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:-4.44|P-value:4.44E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTG1(YOL067C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SEA4(YBL104C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SET3(YKR029C)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SOD1(YJR104C)|FD-Score:-3.77|P-value:8.28E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPE4(YLR146C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SSE1(YPL106C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SYN8(YAL014C)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TOF1(YNL273W)|FD-Score:-3.92|P-value:4.40E-5||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TPK2(YPL203W)|FD-Score:-4.37|P-value:6.35E-6||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TPN1(YGL186C)|FD-Score:-5.37|P-value:3.99E-8||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TSR2(YLR435W)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBP13(YBL067C)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UBX2(YML013W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VMA1(YDL185W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA4(YOR332W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS27(YNR006W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS3(YDR495C)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS55(YJR044C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS64(YDR200C)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YBR027C(YBR027C_d)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR225W(YBR225W_p)|FD-Score:-3.14|P-value:8.37E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components Gene:YCR061W(YCR061W_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDL242W(YDL242W_d)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER066C-A(YER066C-A_d)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL176C(YGL176C_p)|FD-Score:-4.98|P-value:3.14E-7||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR250C(YGR250C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YJR038C(YJR038C_d)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YME2(YMR302C)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YNL296W(YNL296W_d)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOR052C(YOR052C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR053W(YOR053W_d)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR296W(YOR296W_p)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YPL113C(YPL113C_p)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:ACF4(YJR083C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Protein of unknown function, computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate Gene:ADR1(YDR216W)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AEP1(YMR064W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Protein required for expression of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0 ATP synthase Gene:AIP1(YMR092C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress Gene:ALG6(YOR002W)|FD-Score:3.36|P-value:3.88E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:APS1(YLR170C)|FD-Score:4|P-value:3.14E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARG4(YHR018C)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:BNA4(YBL098W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:BNI1(YNL271C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BUD16(YEL029C)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; similarity to yeast BUD17 and human pyridoxal kinase (PDXK) Gene:CSF1(YLR087C)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DBF2(YGR092W)|FD-Score:4.27|P-value:9.83E-6||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DBF20(YPR111W)|FD-Score:-3.15|P-value:8.17E-4||SGD DESC:Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs Gene:DBP7(YKR024C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DDR2(YOL052C-A)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication Gene:EAF6(YJR082C)|FD-Score:-3.69|P-value:1.12E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:GEA2(YEL022W)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HEL1(YKR017C_p)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HNM1(YGL077C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol Gene:ICE2(YIL090W)|FD-Score:-4.21|P-value:1.25E-5||SGD DESC:Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells Gene:IFM1(YOL023W)|FD-Score:5.49|P-value:2.02E-8||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IPK1(YDR315C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:LCB4(YOR171C)|FD-Score:4.4|P-value:5.35E-6||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:MET17(YLR303W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MF(ALPHA)1(YPL187W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor Gene:NCR1(YPL006W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:OAF3(YKR064W)|FD-Score:-3.2|P-value:6.92E-4||SGD DESC:Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:PDE1(YGL248W)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Low-affinity cyclic AMP phosphodiesterase, controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation Gene:PDR3(YBL005W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:PEX11(YOL147C)|FD-Score:3.96|P-value:3.69E-5||SGD DESC:Peroxisomal membrane protein required for medium-chain fatty acid oxidation and peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p Gene:PFK26(YIL107C)|FD-Score:3.31|P-value:4.64E-4||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PRY1(YJL079C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:REG2(YBR050C)|FD-Score:3.82|P-value:6.64E-5||SGD DESC:Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease Gene:RPL24B(YGR148C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication Gene:RPS29A(YLR388W)|FD-Score:4.01|P-value:3.10E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:-4.44|P-value:4.44E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RSM25(YIL093C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTG1(YOL067C)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SEA4(YBL104C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SET3(YKR029C)|FD-Score:-3.38|P-value:3.69E-4||SGD DESC:Defining member of the SET3 histone deacetylase complex which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication Gene:SOD1(YJR104C)|FD-Score:-3.77|P-value:8.28E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPE4(YLR146C)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SSE1(YPL106C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication Gene:SYN8(YAL014C)|FD-Score:-3.27|P-value:5.43E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TOF1(YNL273W)|FD-Score:-3.92|P-value:4.40E-5||SGD DESC:Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase Gene:TPK2(YPL203W)|FD-Score:-4.37|P-value:6.35E-6||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TPN1(YGL186C)|FD-Score:-5.37|P-value:3.99E-8||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TSR2(YLR435W)|FD-Score:3.39|P-value:3.56E-4||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBP13(YBL067C)|FD-Score:-3.18|P-value:7.25E-4||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UBX2(YML013W)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VMA1(YDL185W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA4(YOR332W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress Gene:VPS27(YNR006W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS3(YDR495C)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS55(YJR044C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:VPS64(YDR200C)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YBR027C(YBR027C_d)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR225W(YBR225W_p)|FD-Score:-3.14|P-value:8.37E-4||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components Gene:YCR061W(YCR061W_p)|FD-Score:3.35|P-value:4.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDL242W(YDL242W_d)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER066C-A(YER066C-A_d)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YGL176C(YGL176C_p)|FD-Score:-4.98|P-value:3.14E-7||SGD DESC:Putative protein of unknown function; deletion mutant is viable and has no detectable phenotype Gene:YGR250C(YGR250C)|FD-Score:3.23|P-value:6.19E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YJR038C(YJR038C_d)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YME2(YMR302C)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YNL296W(YNL296W_d)|FD-Score:3.79|P-value:7.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOR052C(YOR052C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOR053W(YOR053W_d)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C Gene:YOR296W(YOR296W_p)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene Gene:YPL113C(YPL113C_p)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOL077C4.191.42E-50.39BRX1Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
YKL035W3.807.20E-50.19UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YGL123W3.611.55E-40.21RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YJL019W3.393.46E-40.06MPS3Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
YPL007C3.344.23E-40.06TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YNR035C3.285.25E-40.33ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YDR172W2.950.001590.03SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YBL023C2.920.001760.07MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YKL152C2.850.002180.03GPM1Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis
YGR114C_d2.820.002410.06YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YPL128C2.760.002920.05TBF1Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
YDR288W2.700.003440.07NSE3Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
YIR006C2.640.004190.01PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease
YEL002C2.620.004350.05WBP1Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
YOR103C2.570.005030.02OST2Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL023W5.492.02E-8IFM1Mitochondrial translation initiation factor 2
YLR087C5.151.31E-7CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YOL052C-A4.993.06E-7DDR2Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
YMR302C4.621.96E-6YME2Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases
YDR200C4.523.05E-6VPS64Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance
YOR171C4.405.35E-6LCB4Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes
YGR092W4.279.83E-6DBF2Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis
YDR315C4.261.03E-5IPK1Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
YML013W4.251.08E-5UBX2Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis
YDL185W4.161.61E-5VMA1Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
YPL106C4.121.88E-5SSE1ATPase component of the heat shock protein Hsp90 chaperone complex; binds unfolded proteins; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSE1 has a paralog, SSE2, that arose from the whole genome duplication
YDR216W4.102.07E-5ADR1Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization
YJL079C4.072.35E-5PRY1Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication
YOR053W_d4.013.03E-5YOR053W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C
YLR388W4.013.10E-5RPS29AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1571
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0773.10E-9SGTC_1290088-0017 176.6 μMChemDiv (Drug-like library)1824460.126761
0.0673.09E-7SGTC_9011112-0062 60.1 μMChemDiv (Drug-like library)19084570.144928
0.0655.66E-7SGTC_1859st055998 20.0 μMTimTec (Natural product derivative library)6763010.125
0.0641.02E-6SGTC_1591genipin 88.4 μMTimTec (Pure natural product library)4424240.105263mitochondrial processes
0.0621.64E-6SGTC_1555citronellol 128.0 μMTimTec (Pure natural product library)88420.0307692
0.0604.10E-6SGTC_20405100729 123.0 μMChembridge (Fragment library)118400.131148
0.0588.94E-6SGTC_26373-alpha-acetoxydihydrodeoxygedunin 100.0 μMMicrosource (Natural product library)67085140.105263
0.0561.74E-5SGTC_15427-hydroxy-3'-methoxyflavone 49.7 μMTimTec (Pure natural product library)53931530.307692
0.0561.84E-5SGTC_24525863136 48.4 μMMiscellaneous21840960.0933333
0.0543.72E-5SGTC_1309pyrazolanthrone 13.4 μMChemDiv (Drug-like library)85150.0895522
0.0534.90E-5SGTC_13963511-0007 98.6 μMChemDiv (Drug-like library)X13960.0543478mitochondrial processes
0.0525.53E-5SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0641026
0.0526.46E-5SGTC_29619083290 32.5 μMChembridge (Drug-like library)177414900.0958904
0.0526.79E-5SGTC_790052-0007 94.1 μMChemDiv (Drug-like library)28014720.084507
0.0511.04E-4SGTC_31309127414 49.5 μMChembridge (Drug-like library)14946460.0898876

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_15447-methoxy-4-methylcoumarin105 μM0.5390807TimTec (Pure natural product library)190.19532.32903
SGTC_1912st06020990.8 μM0.481481688787TimTec (Natural product derivative library)220.221281.9204
SGTC_1903st06021178.3 μM0.403509688960TimTec (Natural product derivative library)220.221281.9204
SGTC_1904st06022578 μM0.39285766595TimTec (Natural product derivative library)204.221882.67803
SGTC_1735st03431056.75 μM0.385965871153TimTec (Natural product derivative library)293.530483.13403Golgi
SGTC_1867st05737264 μM0.385965688837TimTec (Natural product derivative library)312.316643.08705
SGTC_15666,7-dimethoxy-4-ethylcoumarin85.4 μM0.381818688761TimTec (Pure natural product library)234.247862.76904
SGTC_1887st05764870.8 μM0.372881688672TimTec (Natural product derivative library)282.290663.10304
SGTC_1909st06083751.5 μM0.360656678937TimTec (Natural product derivative library)266.291263.7103
SGTC_18693',7'-dimethoxyflavone40.3 μM0.35688664TimTec (Natural product derivative library)282.290662.80804