epiandrosterone

(3S,5S,8R,9S,10S,13S,14S)-3-hydroxy-10,13-dimethyl-1,2,3,4,5,6,7,8,9,11,12,14,15,16-tetradecahydrocyclopenta[a]phenanthren-17-one




Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1572
Screen concentration 68.9 μM
Source TimTec (Pure natural product library)
PubChem CID 441302
SMILES CC12CCC(CC1CCC3C2CCC4(C3CCC4=O)C)O
Standardized SMILES CC12CCC(O)CC1CCC3C2CCC4(C)C3CCC4=O
Molecular weight 290.4403
ALogP 3.59
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.22
% growth inhibition (Hom. pool) 9.43


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 441302
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.12||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CCT5(YJR064W)|FD-Score:-4.33|P-value:7.29E-6|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.09||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:IMP4(YNL075W)|FD-Score:-3.21|P-value:6.57E-4|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:MTR3(YGR158C)|FD-Score:-4.32|P-value:7.84E-6|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NSE4(YDL105W)|FD-Score:3.23|P-value:6.17E-4|Clearance:0.03||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RPC17(YJL011C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPC53(YDL150W)|FD-Score:-3.31|P-value:4.74E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RSC6(YCR052W)|FD-Score:-3.83|P-value:6.32E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:TEL2(YGR099W)|FD-Score:4.4|P-value:5.36E-6|Clearance:0.76||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TSR1(YDL060W)|FD-Score:3.2|P-value:6.77E-4|Clearance:0.01||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UTP14(YML093W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.19||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDR187C(YDR187C_d)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YIF1(YNL263C)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 Gene:YJL195C(YJL195C_d)|FD-Score:4.2|P-value:1.31E-5|Clearance:0.76||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YPL238C(YPL238C_d)|FD-Score:-3.35|P-value:4.06E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:BMS1(YPL217C)|FD-Score:3.35|P-value:4.08E-4|Clearance:0.12||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CCT5(YJR064W)|FD-Score:-4.33|P-value:7.29E-6|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC20(YGL116W)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.09||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:IMP4(YNL075W)|FD-Score:-3.21|P-value:6.57E-4|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:MTR3(YGR158C)|FD-Score:-4.32|P-value:7.84E-6|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NSE4(YDL105W)|FD-Score:3.23|P-value:6.17E-4|Clearance:0.03||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RPC17(YJL011C)|FD-Score:-3.71|P-value:1.03E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPC53(YDL150W)|FD-Score:-3.31|P-value:4.74E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RSC6(YCR052W)|FD-Score:-3.83|P-value:6.32E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:TEL2(YGR099W)|FD-Score:4.4|P-value:5.36E-6|Clearance:0.76||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:TSR1(YDL060W)|FD-Score:3.2|P-value:6.77E-4|Clearance:0.01||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:UTP14(YML093W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0.19||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDR187C(YDR187C_d)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YIF1(YNL263C)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 Gene:YJL195C(YJL195C_d)|FD-Score:4.2|P-value:1.31E-5|Clearance:0.76||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6 Gene:YPL238C(YPL238C_d)|FD-Score:-3.35|P-value:4.06E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 441302
Download HOP data (tab-delimited text)  (excel)
Gene:ADR1(YDR216W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AFT1(YGL071W)|FD-Score:4.52|P-value:3.14E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARO1(YDR127W)|FD-Score:10.1|P-value:2.62E-24||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:8.43|P-value:1.79E-17||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATE1(YGL017W)|FD-Score:3.77|P-value:8.20E-5||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:ATP5(YDR298C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BCK1(YJL095W)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUG1(YDL099W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Gene:CBF1(YJR060W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CIN1(YOR349W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CST9(YLR394W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:DOS2(YDR068W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ERG2(YMR202W)|FD-Score:5.55|P-value:1.40E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GCN2(YDR283C)|FD-Score:11.6|P-value:3.60E-31||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:13.4|P-value:2.20E-41||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:7.33|P-value:1.17E-13||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCS1(YDL226C)|FD-Score:4.78|P-value:8.60E-7||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GIS1(YDR096W)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication Gene:GPR1(YDL035C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:IPK1(YDR315C)|FD-Score:3.85|P-value:6.00E-5||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:KEX1(YGL203C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:MIG1(YGL035C)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MND2(YIR025W)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MUS81(YDR386W)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NGL3(YML118W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication Gene:NGR1(YBR212W)|FD-Score:-4.08|P-value:2.30E-5||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NQM1(YGR043C)|FD-Score:3.88|P-value:5.23E-5||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:OSH2(YDL019C)|FD-Score:3.81|P-value:7.07E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEF1(YGR058W)|FD-Score:4.83|P-value:6.69E-7||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PET130(YJL023C)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PFK26(YIL107C)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PKR1(YMR123W)|FD-Score:-4.53|P-value:2.95E-6||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PPA2(YMR267W)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PRS3(YHL011C)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PSY4(YBL046W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:RGP1(YDR137W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:ROM1(YGR070W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:ROM2(YLR371W)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPN4(YDL020C)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SPE4(YLR146C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SSD1(YDR293C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN8(YNL025C)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:THI3(YDL080C)|FD-Score:4.33|P-value:7.47E-6||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TOM1(YDR457W)|FD-Score:-4.37|P-value:6.34E-6||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOS6(YNL300W_p)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TPK1(YJL164C)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:12.7|P-value:1.87E-37||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:17.4|P-value:4.84E-68||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:17.2|P-value:1.12E-66||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:12.9|P-value:2.32E-38||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:8.87|P-value:3.75E-19||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBP9(YER098W)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:UGA2(YBR006W)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VTS1(YOR359W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YDL073W(YDL073W_p)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:7.33|P-value:1.16E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR306C(YDR306C_p)|FD-Score:10.5|P-value:4.24E-26||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YDR442W(YDR442W_d)|FD-Score:4.29|P-value:9.06E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:5.48|P-value:2.07E-8||SGD DESC:Putative protein of unknown function Gene:YGL118C(YGL118C_d)|FD-Score:5.33|P-value:4.88E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR012W(YGR012W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Putative cysteine synthase, localized to the mitochondrial outer membrane Gene:YGR201C(YGR201C_p)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Putative protein of unknown function Gene:YKL030W(YKL030W_d)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YLL007C(YLL007C_p)|FD-Score:3.73|P-value:9.70E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR287C(YLR287C_p)|FD-Score:5.09|P-value:1.79E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YML018C(YML018C_p)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR193C-A(YMR193C-A_d)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR225W(YOR225W_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ADR1(YDR216W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:AFT1(YGL071W)|FD-Score:4.52|P-value:3.14E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARO1(YDR127W)|FD-Score:10.1|P-value:2.62E-24||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:8.43|P-value:1.79E-17||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ATE1(YGL017W)|FD-Score:3.77|P-value:8.20E-5||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:ATP5(YDR298C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated Gene:BCK1(YJL095W)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUG1(YDL099W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes Gene:CBF1(YJR060W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CIN1(YOR349W)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CST9(YLR394W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:DOS2(YDR068W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ERG2(YMR202W)|FD-Score:5.55|P-value:1.40E-8||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GCN2(YDR283C)|FD-Score:11.6|P-value:3.60E-31||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.65|P-value:1.69E-6||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:13.4|P-value:2.20E-41||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:7.33|P-value:1.17E-13||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCS1(YDL226C)|FD-Score:4.78|P-value:8.60E-7||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GIS1(YDR096W)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication Gene:GPR1(YDL035C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:IPK1(YDR315C)|FD-Score:3.85|P-value:6.00E-5||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:KEX1(YGL203C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins Gene:MIG1(YGL035C)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion Gene:MND2(YIR025W)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MUS81(YDR386W)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p Gene:NGL3(YML118W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication Gene:NGR1(YBR212W)|FD-Score:-4.08|P-value:2.30E-5||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NQM1(YGR043C)|FD-Score:3.88|P-value:5.23E-5||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:OSH2(YDL019C)|FD-Score:3.81|P-value:7.07E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEF1(YGR058W)|FD-Score:4.83|P-value:6.69E-7||SGD DESC:Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly Gene:PET130(YJL023C)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PFK26(YIL107C)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:6-phosphofructo-2-kinase, inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A Gene:PKR1(YMR123W)|FD-Score:-4.53|P-value:2.95E-6||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PPA2(YMR267W)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate Gene:PRS3(YHL011C)|FD-Score:-3.21|P-value:6.67E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PSY4(YBL046W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2 Gene:RGP1(YDR137W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:ROM1(YGR070W)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:ROM2(YLR371W)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPN4(YDL020C)|FD-Score:-3.89|P-value:4.94E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SPE4(YLR146C)|FD-Score:3.34|P-value:4.16E-4||SGD DESC:Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid Gene:SSD1(YDR293C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSN8(YNL025C)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Cyclin-like component of the RNA polymerase II holoenzyme, involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression and telomere maintenance Gene:THI3(YDL080C)|FD-Score:4.33|P-value:7.47E-6||SGD DESC:Probable alpha-ketoisocaproate decarboxylase, may have a role in catabolism of amino acids to long-chain and complex alcohols; required for expression of enzymes involved in thiamine biosynthesis Gene:TOM1(YDR457W)|FD-Score:-4.37|P-value:6.34E-6||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOS6(YNL300W_p)|FD-Score:-3.5|P-value:2.33E-4||SGD DESC:Glycosylphosphatidylinositol-dependent cell wall protein, expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid Gene:TPK1(YJL164C)|FD-Score:-3.9|P-value:4.73E-5||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:12.7|P-value:1.87E-37||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:17.4|P-value:4.84E-68||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:17.2|P-value:1.12E-66||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:12.9|P-value:2.32E-38||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:8.87|P-value:3.75E-19||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBP9(YER098W)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:UGA2(YBR006W)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VTS1(YOR359W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YDL073W(YDL073W_p)|FD-Score:3.67|P-value:1.24E-4||SGD DESC:Putative protein of unknown function; YDL073W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:7.33|P-value:1.16E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR306C(YDR306C_p)|FD-Score:10.5|P-value:4.24E-26||SGD DESC:F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain Gene:YDR442W(YDR442W_d)|FD-Score:4.29|P-value:9.06E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:5.48|P-value:2.07E-8||SGD DESC:Putative protein of unknown function Gene:YGL118C(YGL118C_d)|FD-Score:5.33|P-value:4.88E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR012W(YGR012W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Putative cysteine synthase, localized to the mitochondrial outer membrane Gene:YGR201C(YGR201C_p)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Putative protein of unknown function Gene:YKL030W(YKL030W_d)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YLL007C(YLL007C_p)|FD-Score:3.73|P-value:9.70E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR287C(YLR287C_p)|FD-Score:5.09|P-value:1.79E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YML018C(YML018C_p)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR193C-A(YMR193C-A_d)|FD-Score:-3.17|P-value:7.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR225W(YOR225W_d)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR099W4.405.36E-60.76TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YJL195C_d4.201.31E-50.76YJL195C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
YGL116W3.442.90E-40.09CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YPL217C3.354.08E-40.12BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YDL105W3.236.17E-40.03NSE4Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YDL060W3.206.77E-40.01TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YML093W3.197.13E-40.19UTP14Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YDL103C3.000.001370.10QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YCL017C2.900.001890.05NFS1Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
YPL016W2.850.002190.06SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YDL195W2.790.002640.11SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YAL032C2.670.003740.02PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YKL058W2.650.004010.10TOA2TFIIA small subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to smallest subunit of human and Drosophila TFIIA; protein abundance increases in response to DNA replication stress
YDL108W2.550.005451.97E-4KIN28Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters
YOR217W2.550.005450.11RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER090W17.404.84E-68TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YKL211C17.201.12E-66TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YKR026C13.402.20E-41GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YDR354W12.902.32E-38TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR007W12.701.87E-37TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR283C11.603.60E-31GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YDR306C_p10.504.24E-26YDR306C_pF-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
YDR127W10.102.62E-24ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YGL026C8.873.75E-19TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YGL148W8.431.79E-17ARO2Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
YDR008C_d7.331.16E-13YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YEL009C7.331.17E-13GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YMR202W5.551.40E-8ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YGL117W_p5.482.07E-8YGL117W_pPutative protein of unknown function
YGL118C_d5.334.88E-8YGL118C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1572
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.6170SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.52
0.5720SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.52
0.5560SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0597015
0.5500SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0864198
0.5420SGTC_18825615643 20.0 μMMiscellaneous22530750.047619TRP & mitochondrial translation
0.5410SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0526316
0.5390SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413021
0.5300SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.0806452plasma membrane duress
0.5220SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.283784
0.5220SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.166667
0.5100SGTC_33089128332 17.6 μMChembridge (Drug-like library)173328050.0125plasma membrane duress
0.5020SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.0493827
0.5010SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.057971
0.5010SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.0588235
0.4980SGTC_24785763493 32.3 μMMiscellaneous13655760.056338

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2540epiandrosterone100 μM1441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM12754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_25005-alpha-androstan-3,17-dione61.44 μM0.6444446710655Microsource (Natural product library)288.424423.40202
SGTC_2650prasterone100 μM0.5216759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.525881Miscellaneous288.424423.33812
SGTC_1560lithocholic acid53.1 μM0.4736849903TimTec (Pure natural product library)376.57265.18423
SGTC_1974st06944826.9 μM0.4074076323587TimTec (Natural product derivative library)305.454943.36923
SGTC_406estrone100 μM0.3859655870Miscellaneous270.366083.93812
SGTC_1519esterone74 μM0.385965698TimTec (Pure natural product library)270.366083.93812DNA intercalators
SGTC_1517deoxycholic acid methyl ester49.2 μM0.378788229346TimTec (Pure natural product library)406.598584.30724