erythromycin ethylsuccinate FDA approved compound

4-O-[4-(dimethylamino)-2-[[14-ethyl-7,12,13-trihydroxy-4-(5-hydroxy-4-methoxy-4,6-dimethyloxan-2-yl)oxy-3,5,7,9,11,13-hexamethyl-2,10-dioxo-oxacyclotetradec-6-yl]oxy]-6-methyloxan-3-yl] 1-O-ethyl butanedioate

An antibiotic and ribosome inhibitor.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Anti-Bacterial Agents;Enzyme Inhibitors;Gastrointestinal Agents;Protein Synthesis Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1576
Screen concentration 23.2 μM
Source TimTec (Pure natural product library)
PubChem CID 3256
SMILES CCC1C(C(C(C(=O)C(CC(C(C(C(C(C(=O)O1)C)OC2CC(C(C(O2)C)O)(C)OC)C)OC3C(C(CC(O3)C)N(C)C)OC(=O)CCC(=O)OCC)(C)O)C)C)O)(C)O
Standardized SMILES CCOC(=O)CCC(=O)OC1C(OC2C(C)C(OC3CC(C)(OC)C(O)C(C)O3)C(C)C(=O)OC(CC)C(C)(O)C(O)C(C)C(=O)C(C)CC2(C)O)OC(C)CC1N(C)C
Molecular weight 862.0527
ALogP 2.59
H-bond donor count 4
H-bond acceptor count 17
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.14
% growth inhibition (Hom. pool) -0.03


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 3256
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:-3.14|P-value:8.50E-4|Clearance:0||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:CSE4(YKL049C)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.42||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:DIS3(YOL021C)|FD-Score:-3.26|P-value:5.53E-4|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ERG25(YGR060W)|FD-Score:-3.9|P-value:4.75E-5|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:FAS1(YKL182W)|FD-Score:-3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:KRR1(YCL059C)|FD-Score:-3.81|P-value:6.99E-5|Clearance:0||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:OLE1(YGL055W)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.29||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PRP3(YDR473C)|FD-Score:4.46|P-value:4.04E-6|Clearance:0.31||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PTI1(YGR156W)|FD-Score:3.28|P-value:5.17E-4|Clearance:0.02||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPC40(YPR110C)|FD-Score:-3.86|P-value:5.65E-5|Clearance:0||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RPO26(YPR187W)|FD-Score:4.16|P-value:1.61E-5|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRB1(YMR131C)|FD-Score:-3.55|P-value:1.93E-4|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RSC58(YLR033W)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.27||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEN2(YLR105C)|FD-Score:-3.19|P-value:7.19E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Gene:SLN1(YIL147C)|FD-Score:5.24|P-value:8.01E-8|Clearance:0.78||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:TAF7(YMR227C)|FD-Score:6.93|P-value:2.11E-12|Clearance:1.69||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TFC3(YAL001C)|FD-Score:-3.26|P-value:5.65E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:UBA2(YDR390C)|FD-Score:-3.14|P-value:8.40E-4|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:UTP22(YGR090W)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.13||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:ACS2(YLR153C)|FD-Score:-3.14|P-value:8.50E-4|Clearance:0||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:CSE4(YKL049C)|FD-Score:4.13|P-value:1.85E-5|Clearance:0.42||SGD DESC:Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions Gene:DIS3(YOL021C)|FD-Score:-3.26|P-value:5.53E-4|Clearance:0||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ERG25(YGR060W)|FD-Score:-3.9|P-value:4.75E-5|Clearance:0||SGD DESC:C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol Gene:FAS1(YKL182W)|FD-Score:-3.13|P-value:8.87E-4|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:KRR1(YCL059C)|FD-Score:-3.81|P-value:6.99E-5|Clearance:0||SGD DESC:Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit Gene:OLE1(YGL055W)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.29||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PRP3(YDR473C)|FD-Score:4.46|P-value:4.04E-6|Clearance:0.31||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:PTI1(YGR156W)|FD-Score:3.28|P-value:5.17E-4|Clearance:0.02||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPC40(YPR110C)|FD-Score:-3.86|P-value:5.65E-5|Clearance:0||SGD DESC:RNA polymerase subunit AC40, common to RNA polymerase I and III Gene:RPO26(YPR187W)|FD-Score:4.16|P-value:1.61E-5|Clearance:0.03||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRB1(YMR131C)|FD-Score:-3.55|P-value:1.93E-4|Clearance:0||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:RSC58(YLR033W)|FD-Score:3.26|P-value:5.52E-4|Clearance:0.27||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SEN2(YLR105C)|FD-Score:-3.19|P-value:7.19E-4|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease Gene:SLN1(YIL147C)|FD-Score:5.24|P-value:8.01E-8|Clearance:0.78||SGD DESC:Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators Gene:TAF7(YMR227C)|FD-Score:6.93|P-value:2.11E-12|Clearance:1.69||SGD DESC:TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation Gene:TFC3(YAL001C)|FD-Score:-3.26|P-value:5.65E-4|Clearance:0||SGD DESC:Largest of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauB domain of TFIIIC that binds DNA at the BoxB promoter sites of tRNA and similar genes; cooperates with Tfc6p in DNA binding Gene:UBA2(YDR390C)|FD-Score:-3.14|P-value:8.40E-4|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:UTP22(YGR090W)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.13||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 3256
Download HOP data (tab-delimited text)  (excel)
Gene:AHC1(YOR023C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ATG17(YLR423C)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:BIO3(YNR058W)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CDC10(YCR002C)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CHS5(YLR330W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CNN1(YFR046C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:CTI6(YPL181W)|FD-Score:4.33|P-value:7.42E-6||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DIA1(YMR316W)|FD-Score:3.77|P-value:8.16E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DIT1(YDR403W)|FD-Score:-4.43|P-value:4.80E-6||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:DUG2(YBR281C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAF6(YJR082C)|FD-Score:-4.99|P-value:3.06E-7||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:EGD2(YHR193C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:FHN1(YGR131W)|FD-Score:-4.39|P-value:5.56E-6||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FIS1(YIL065C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:GND2(YGR256W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:HEH2(YDR458C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HIS2(YFR025C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HXT14(YNL318C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:IBA57(YJR122W)|FD-Score:5.64|P-value:8.49E-9||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IMA1(YGR287C)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IRS4(YKR019C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:-3.89|P-value:5.10E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MET13(YGL125W)|FD-Score:-3.96|P-value:3.72E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MRE11(YMR224C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRP17(YKL003C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MRPL23(YOR150W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS28(YDR337W)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSD1(YPL104W)|FD-Score:6.24|P-value:2.15E-10||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NCR1(YPL006W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NFI1(YOR156C)|FD-Score:7.7|P-value:6.67E-15||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NUP2(YLR335W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OPI1(YHL020C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OXP1(YKL215C)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PCK1(YKR097W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PET111(YMR257C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PKH2(YOL100W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PRO2(YOR323C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RIM1(YCR028C-A)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RIM21(YNL294C)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:ROY1(YMR258C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL19B(YBL027W)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPS0A(YGR214W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS29A(YLR388W)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RRF1(YHR038W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RSM25(YIL093C)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAS5(YOR213C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SFB3(YHR098C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SRB8(YCR081W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSF1(YHR066W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SWS2(YNL081C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:SYT1(YPR095C)|FD-Score:3.75|P-value:9.00E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TRI1(YMR233W)|FD-Score:4.88|P-value:5.25E-7||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRR2(YHR106W)|FD-Score:4.99|P-value:2.97E-7||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:UBP12(YJL197W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VAC17(YCL063W)|FD-Score:5.05|P-value:2.22E-7||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VBA1(YMR088C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS71(YML041C)|FD-Score:4.93|P-value:4.10E-7||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAR047C(YAR047C_d)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR134W(YBR134W_d)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCK3(YER123W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCL049C(YCL049C_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YCR016W(YCR016W_p)|FD-Score:4.45|P-value:4.29E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YER053C-A(YER053C-A_p)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YKL030W(YKL030W_d)|FD-Score:-3.98|P-value:3.45E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YLR211C(YLR211C_p)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YML018C(YML018C_p)|FD-Score:5.44|P-value:2.64E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR001C-A(YMR001C-A_p)|FD-Score:9.65|P-value:2.58E-22||SGD DESC:Putative protein of unknown function Gene:YMR034C(YMR034C_p)|FD-Score:-4.21|P-value:1.26E-5||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR196W(YMR196W_p)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL095C(YNL095C_p)|FD-Score:4.71|P-value:1.22E-6||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YNL108C(YNL108C_p)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNR064C(YNR064C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOR012W(YOR012W_p)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Putative protein of unknown function Gene:YOR263C(YOR263C_d)|FD-Score:5.51|P-value:1.83E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YOR343C(YOR343C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL257W(YPL257W_p)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:AHC1(YOR023C)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ATG17(YLR423C)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:BIO3(YNR058W)|FD-Score:4.49|P-value:3.58E-6||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:CDC10(YCR002C)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CHS5(YLR330W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CNN1(YFR046C)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:CTI6(YPL181W)|FD-Score:4.33|P-value:7.42E-6||SGD DESC:Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain Gene:DIA1(YMR316W)|FD-Score:3.77|P-value:8.16E-5||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DIT1(YDR403W)|FD-Score:-4.43|P-value:4.80E-6||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:DUG2(YBR281C)|FD-Score:3.1|P-value:9.52E-4||SGD DESC:Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EAF6(YJR082C)|FD-Score:-4.99|P-value:3.06E-7||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:EGD2(YHR193C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:FHN1(YGR131W)|FD-Score:-4.39|P-value:5.56E-6||SGD DESC:Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain Gene:FIS1(YIL065C)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:GND2(YGR256W)|FD-Score:3.38|P-value:3.69E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication Gene:HEH2(YDR458C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HIS2(YFR025C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HXT14(YNL318C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:IBA57(YJR122W)|FD-Score:5.64|P-value:8.49E-9||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IMA1(YGR287C)|FD-Score:3.91|P-value:4.69E-5||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IRS4(YKR019C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:-3.89|P-value:5.10E-5||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MET13(YGL125W)|FD-Score:-3.96|P-value:3.72E-5||SGD DESC:Major isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway Gene:MRE11(YMR224C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; exhibits nuclease activity that appears to be required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress Gene:MRP17(YKL003C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MRPL23(YOR150W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS28(YDR337W)|FD-Score:4.64|P-value:1.74E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSD1(YPL104W)|FD-Score:6.24|P-value:2.15E-10||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:NCR1(YPL006W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Vacuolar membrane protein that transits through the biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; glycoprotein and functional orthologue of human Niemann Pick C1 (NPC1) protein Gene:NFI1(YOR156C)|FD-Score:7.7|P-value:6.67E-15||SGD DESC:SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length Gene:NUP2(YLR335W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OPI1(YHL020C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Transcriptional regulator of a variety of genes; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance Gene:OXP1(YKL215C)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PCK1(YKR097W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PET111(YMR257C)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PKH2(YOL100W)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p Gene:PRO2(YOR323C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RIM1(YCR028C-A)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication Gene:RIM21(YNL294C)|FD-Score:-3.26|P-value:5.63E-4||SGD DESC:pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH Gene:ROY1(YMR258C)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL19B(YBL027W)|FD-Score:4.49|P-value:3.52E-6||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPS0A(YGR214W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:RPS29A(YLR388W)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:RRF1(YHR038W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria Gene:RSM25(YIL093C)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAS5(YOR213C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SFB3(YHR098C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SRB8(YCR081W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression Gene:SSF1(YHR066W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SWS2(YNL081C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:SYT1(YPR095C)|FD-Score:3.75|P-value:9.00E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TRI1(YMR233W)|FD-Score:4.88|P-value:5.25E-7||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRR2(YHR106W)|FD-Score:4.99|P-value:2.97E-7||SGD DESC:Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication Gene:UBP12(YJL197W)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VAC17(YCL063W)|FD-Score:5.05|P-value:2.22E-7||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VBA1(YMR088C)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS71(YML041C)|FD-Score:4.93|P-value:4.10E-7||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAR047C(YAR047C_d)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR134W(YBR134W_d)|FD-Score:3.14|P-value:8.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCK3(YER123W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YCL049C(YCL049C_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene Gene:YCR016W(YCR016W_p)|FD-Score:4.45|P-value:4.29E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YER053C-A(YER053C-A_p)|FD-Score:3.78|P-value:7.88E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YKL030W(YKL030W_d)|FD-Score:-3.98|P-value:3.45E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YLR211C(YLR211C_p)|FD-Score:-3.12|P-value:9.16E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YML018C(YML018C_p)|FD-Score:5.44|P-value:2.64E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR001C-A(YMR001C-A_p)|FD-Score:9.65|P-value:2.58E-22||SGD DESC:Putative protein of unknown function Gene:YMR034C(YMR034C_p)|FD-Score:-4.21|P-value:1.26E-5||SGD DESC:Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene Gene:YMR196W(YMR196W_p)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL095C(YNL095C_p)|FD-Score:4.71|P-value:1.22E-6||SGD DESC:Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication Gene:YNL108C(YNL108C_p)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication Gene:YNR064C(YNR064C)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOR012W(YOR012W_p)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Putative protein of unknown function Gene:YOR263C(YOR263C_d)|FD-Score:5.51|P-value:1.83E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YOR343C(YOR343C_d)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL257W(YPL257W_p)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR227C6.932.11E-121.69TAF7TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
YIL147C5.248.01E-80.78SLN1Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
YDR473C4.464.04E-60.31PRP3Splicing factor, component of the U4/U6-U5 snRNP complex
YPR187W4.161.61E-50.03RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YKL049C4.131.85E-50.42CSE4Centromere protein that resembles histone H3; required for proper kinetochore function; homolog of human CENP-A; levels are regulated by E3 ubiquitin ligase Psh1p; Cse4p marks a subset of open chromatin; associated with promoters, accessible chromatin and RNA polymerase II-bound regions
YGL055W3.711.04E-40.29OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YGR090W3.423.18E-40.13UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YGR156W3.285.17E-40.02PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YLR033W3.265.52E-40.27RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YPL142C_d3.000.001370.01YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YGL247W2.980.001430.02BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YBL050W2.960.001520.05SEC17Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP
YAL034W-A2.920.001760.02MTW1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly
YLR430W2.900.001850.12SEN1Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS
YJL086C_d2.780.002680.04YJL086C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR001C-A_p9.652.58E-22YMR001C-A_pPutative protein of unknown function
YOR156C7.706.67E-15NFI1SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length
YPL104W6.242.15E-10MSD1Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
YJR122W5.648.49E-9IBA57Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system
YOR263C_d5.511.83E-8YOR263C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W
YML018C_p5.442.64E-8YML018C_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication
YCL063W5.052.22E-7VAC17Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p
YHR106W4.992.97E-7TRR2Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication
YML041C4.934.10E-7VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YMR233W4.885.25E-7TRI1Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication
YNL095C_p4.711.22E-6YNL095C_pPutative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication
YLR423C4.701.31E-6ATG17Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells
YDR337W4.641.74E-6MRPS28Mitochondrial ribosomal protein of the small subunit
YBL027W4.493.52E-6RPL19BRibosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication
YNR058W4.493.58E-6BIO37,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis

GO enrichment analysis for SGTC_1576
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0863.12E-11SGTC_1994063-0024 41.6 μMChemDiv (Drug-like library)3062150.0614035
0.0699.12E-8SGTC_13261431-2094 10.8 μMChemDiv (Drug-like library)6162780.0173913tubulin folding & SWR complex
0.0699.17E-8SGTC_31499097855 49.5 μMChembridge (Drug-like library)252366250.05Golgi
0.0621.60E-6SGTC_14013807-4679 7.6 μMChemDiv (Drug-like library)32914560.0409836tubulin folding & SWR complex
0.0622.15E-6SGTC_2501sodium tetradecyl sulfate 13.4 μMMicrosource (Natural product library)236657720.0431034
0.0622.21E-6SGTC_1841494-0393 24.7 μMChemDiv (Drug-like library)30906470.0186916
0.0612.63E-6SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0409836RSC complex & mRNA processing
0.0603.71E-6SGTC_1165k072-0275 30.3 μMChemDiv (Drug-like library)61535230.0168067
0.0603.98E-6SGTC_6731082-0474 110.0 μMChemDiv (Drug-like library)30980460.0884956RSC complex & mRNA processing
0.0596.34E-6SGTC_22697935389 174.4 μMChembridge (Fragment library)976050.017094tubulin folding & SWR complex
0.0587.25E-6SGTC_20225136654 200.0 μMChembridge (Fragment library)17137710.0642202
0.0588.42E-6SGTC_2705st078069 42.8 μMTimTec (Natural product derivative library)15300610.046875
0.0571.09E-5SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.0409836mitochondrial processes
0.0571.09E-5SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.0265487heme biosynthesis & mitochondrial translocase
0.0561.67E-5SGTC_1610st001799 48.1 μMTimTec (Natural product derivative library)6218970.0866142

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1578josamycin24.2 μM0.2635145282165TimTec (Pure natural product library)827.994962.957316
SGTC_1574spiramycin23.7 μM0.2587416419898TimTec (Pure natural product library)843.052662.049416
SGTC_2611pyromycin23.93 μM0.230769196990Microsource (Natural product library)585.5991.992512
SGTC_2738aclarubicin5.55 μM0.226027451415Miscellaneous811.867922.682416RNA pol III & RNase P/MRP
SGTC_272doxorubicin8.13 μM0.17647131703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair
SGTC_2747doxorubicin7.63 μM0.17647131703Miscellaneous543.51926-0.044612anthracycline transcription coupled DNA repair
SGTC_1085doxorubicin7.67 μM0.176471443939NIH Clinical Collection579.98020.338712anthracycline transcription coupled DNA repair
SGTC_1105epirubicin18.1 μM0.17647165348NIH Clinical Collection579.98020.338712anthracycline transcription coupled DNA repair
SGTC_1079idarubicin2.36 μM0.173228636362NIH Clinical Collection533.954821.026610DNA damage response
SGTC_257daunorubicin18.5 μM0.17164230323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2662daunorubicin22.37 μM0.17164230323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2746daunorubicin9.88 μM0.17164230323Miscellaneous527.519860.628511anthracycline transcription coupled DNA repair
SGTC_2645abamectin100 μM0.168755702208Microsource (Natural product library)873.076884.692314heme requiring
SGTC_1580hydrocortisone 21-hemisuccinate43.2 μM0.16406216623TimTec (Pure natural product library)462.532661.50738