piperlongumine

1-[(E)-3-(3,4,5-trimethoxyphenyl)prop-2-enoyl]-2,3-dihydropyridin-6-one

Piperlongumine selectively kills cancer cells without harming normal cells. It binds to and inihbits proteins known to regulate oxidative stress, in particular, Glutathione S-transferase pi 1 (GSTP1), increasing the level of reactive oxygen species (ROS) and apoptotic cell death in cancer cells. 

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1596
Screen concentration 63.0 μM
Source TimTec (Pure natural product library)
PubChem CID 637858
SMILES COC1=CC(=CC(=C1OC)OC)C=CC(=O)N2CCC=CC2=O
Standardized SMILES COc1cc(C=CC(=O)N2CCC=CC2=O)cc(OC)c1OC
Molecular weight 317.3365
ALogP 2.14
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.95
% growth inhibition (Hom. pool) 5.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 637858
Download HIP data (tab-delimited text)  (excel)
Gene:ESS1(YJR017C)|FD-Score:-3.22|P-value:6.39E-4|Clearance:0||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:KRE29(YER038C)|FD-Score:-3.4|P-value:3.32E-4|Clearance:0||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MAK5(YBR142W)|FD-Score:5.52|P-value:1.67E-8|Clearance:1.25||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:NAR1(YNL240C)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.44||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NRD1(YNL251C)|FD-Score:4.86|P-value:5.75E-7|Clearance:1.25||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:SCL1(YGL011C)|FD-Score:3.18|P-value:7.49E-4|Clearance:0.17||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SPB1(YCL054W)|FD-Score:-4.1|P-value:2.04E-5|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:YKT6(YKL196C)|FD-Score:-3.37|P-value:3.73E-4|Clearance:0||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YLR076C(YLR076C_d)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:ESS1(YJR017C)|FD-Score:-3.22|P-value:6.39E-4|Clearance:0||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:KRE29(YER038C)|FD-Score:-3.4|P-value:3.32E-4|Clearance:0||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MAK5(YBR142W)|FD-Score:5.52|P-value:1.67E-8|Clearance:1.25||SGD DESC:Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits Gene:NAR1(YNL240C)|FD-Score:3.61|P-value:1.51E-4|Clearance:0.44||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NRD1(YNL251C)|FD-Score:4.86|P-value:5.75E-7|Clearance:1.25||SGD DESC:RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites Gene:SCL1(YGL011C)|FD-Score:3.18|P-value:7.49E-4|Clearance:0.17||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SPB1(YCL054W)|FD-Score:-4.1|P-value:2.04E-5|Clearance:0||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:YKT6(YKL196C)|FD-Score:-3.37|P-value:3.73E-4|Clearance:0||SGD DESC:Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus Gene:YLR076C(YLR076C_d)|FD-Score:-3.58|P-value:1.70E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 637858
Download HOP data (tab-delimited text)  (excel)
Gene:AEP3(YPL005W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:ARG80(YMR042W)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:BMH1(YER177W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CDC55(YGL190C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CTA1(YDR256C)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:DAL7(YIR031C)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DMC1(YER179W)|FD-Score:4.35|P-value:6.73E-6||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DOG1(YHR044C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:EMI2(YDR516C)|FD-Score:4.96|P-value:3.60E-7||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ERG2(YMR202W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FIG2(YCR089W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FKS3(YMR306W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GET1(YGL020C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GID8(YMR135C)|FD-Score:-3.86|P-value:5.62E-5||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GRX8(YLR364W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic Gene:GUP1(YGL084C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HUL5(YGL141W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:HXT1(YHR094C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LSM1(YJL124C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MPT5(YGL178W)|FD-Score:-3.75|P-value:8.95E-5||SGD DESC:mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity Gene:MRPL31(YKL138C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS4(YKR052C)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:NHP6A(YPR052C)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:PAF1(YBR279W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PHB2(YGR231C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PRO2(YOR323C)|FD-Score:4.35|P-value:6.76E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RDS1(YCR106W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:ROM2(YLR371W)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL20B(YOR312C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPS10B(YMR230W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RRG1(YDR065W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RRI2(YOL117W)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RSM25(YIL093C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RUP1(YOR138C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SMK1(YPR054W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SNX4(YJL036W)|FD-Score:-4.63|P-value:1.81E-6||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPO19(YPL130W)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation Gene:TAT1(YBR069C)|FD-Score:-3.92|P-value:4.44E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TED1(YIL039W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TSC3(YBR058C-A)|FD-Score:9.26|P-value:1.01E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX6(YJL048C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:UGX2(YDL169C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:URA10(YMR271C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:VPS36(YLR417W)|FD-Score:4.28|P-value:9.53E-6||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YDR476C(YDR476C_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YGL140C(YGL140C_p)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains Gene:YHL044W(YHL044W_p)|FD-Score:4.53|P-value:2.94E-6||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIG1(YPL201C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YKL169C(YKL169C_d)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKR073C(YKR073C_d)|FD-Score:6.7|P-value:1.06E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR279W(YLR279W_d)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR444C(YLR444C_d)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR015W(YOR015W_d)|FD-Score:-4|P-value:3.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL225W(YPL225W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPL264C(YPL264C_p)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:YPR114W(YPR114W_p)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function Gene:AEP3(YPL005W)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Protein that may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; localized to the matrix face of the mitochondrial inner membrane; stabilizes the bicistronic AAP1-ATP6 mRNA Gene:ARG80(YMR042W)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:BMH1(YER177W)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:CDC55(YGL190C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Non-essential regulatory subunit B of protein phosphatase 2A (PP2A); localization to the cytoplasm requires Zds1p and Zds2p and promotes mitotic entry; localization to the nucleus prevents mitotic exit; required for correct nuclear division and chromosome segregation in meiosis; maintains nucleolar sequestration of Cdc14p during early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion Gene:CTA1(YDR256C)|FD-Score:-3.48|P-value:2.48E-4||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:DAL7(YIR031C)|FD-Score:4.47|P-value:3.89E-6||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DMC1(YER179W)|FD-Score:4.35|P-value:6.73E-6||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DOG1(YHR044C)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:EMI2(YDR516C)|FD-Score:4.96|P-value:3.60E-7||SGD DESC:Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:ERG2(YMR202W)|FD-Score:3.1|P-value:9.70E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:FIG2(YCR089W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating Gene:FKS3(YMR306W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GET1(YGL020C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance Gene:GID8(YMR135C)|FD-Score:-3.86|P-value:5.62E-5||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GRX8(YLR364W)|FD-Score:3.37|P-value:3.79E-4||SGD DESC:Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic Gene:GUP1(YGL084C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HUL5(YGL141W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:HXT1(YHR094C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LSM1(YJL124C)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:MPT5(YGL178W)|FD-Score:-3.75|P-value:8.95E-5||SGD DESC:mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity Gene:MRPL31(YKL138C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL8(YJL063C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRS4(YKR052C)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:NHP6A(YPR052C)|FD-Score:4.43|P-value:4.75E-6||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:PAF1(YBR279W)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PHB2(YGR231C)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation Gene:PRO2(YOR323C)|FD-Score:4.35|P-value:6.76E-6||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RDS1(YCR106W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:ROM2(YLR371W)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL20B(YOR312C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL26B(YGR034W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication Gene:RPL36A(YMR194W)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPS10B(YMR230W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RRG1(YDR065W)|FD-Score:3.5|P-value:2.32E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RRI2(YOL117W)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RSM25(YIL093C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RUP1(YOR138C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SMK1(YPR054W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SNX4(YJL036W)|FD-Score:-4.63|P-value:1.81E-6||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPO19(YPL130W)|FD-Score:4.46|P-value:4.04E-6||SGD DESC:Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation Gene:TAT1(YBR069C)|FD-Score:-3.92|P-value:4.44E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TED1(YIL039W)|FD-Score:3.95|P-value:3.85E-5||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TSC3(YBR058C-A)|FD-Score:9.26|P-value:1.01E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBX6(YJL048C)|FD-Score:3.27|P-value:5.41E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication Gene:UGX2(YDL169C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Protein of unknown function, transcript accumulates in response to any combination of stress conditions Gene:URA10(YMR271C)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:VPS36(YLR417W)|FD-Score:4.28|P-value:9.53E-6||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:YDR476C(YDR476C_p)|FD-Score:3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene Gene:YGL140C(YGL140C_p)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains Gene:YHL044W(YHL044W_p)|FD-Score:4.53|P-value:2.94E-6||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern Gene:YIG1(YPL201C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YKL169C(YKL169C_d)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YKR073C(YKR073C_d)|FD-Score:6.7|P-value:1.06E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR279W(YLR279W_d)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR444C(YLR444C_d)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR015W(YOR015W_d)|FD-Score:-4|P-value:3.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL225W(YPL225W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPL264C(YPL264C_p)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene Gene:YPR114W(YPR114W_p)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR142W5.521.67E-81.25MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
YNL251C4.865.75E-71.25NRD1RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
YNL240C3.611.51E-40.44NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YGL011C3.187.49E-40.17SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YBR190W_d3.000.001340.04YBR190W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
YDL103C2.960.001530.02QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
YGR030C2.940.001650.06POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKL145W2.880.002000.04RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YDL153C2.840.002240.01SAS10Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
YDR373W2.830.002300.07FRQ1N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
YGR267C2.760.002850.07FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
YKL042W2.700.003510.09SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YIL150C2.610.004520.01MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YNR017W2.600.004650.02TIM23Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein import into mitochondrial matrix and inner membrane; with Tim17p, contributes to architecture and function of the import channel
YDR177W2.580.004880.02UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A9.261.01E-20TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YKR073C_d6.701.06E-11YKR073C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR516C4.963.60E-7EMI2Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YHL044W_p4.532.94E-6YHL044W_pPutative integral membrane protein, member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern
YIR031C4.473.89E-6DAL7Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YPL130W4.464.04E-6SPO19Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation
YPR052C4.434.75E-6NHP6AHigh-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YER179W4.356.73E-6DMC1Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p
YOR323C4.356.76E-6PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YLR417W4.289.53E-6VPS36Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
YCR089W4.211.27E-5FIG2Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating
YPL040C4.211.29E-5ISM1Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
YOR035C4.151.69E-5SHE4Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
YGL084C4.131.79E-5GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YPL225W4.102.03E-5YPL225WProtein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_1596
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2452.17E-81SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0746269TSC3-RPN4
0.2361.62E-75SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.126582TSC3-RPN4
0.2228.54E-67SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0789474TSC3-RPN4
0.2137.86E-62SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.084507TSC3-RPN4
0.2132.19E-61SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.15625TSC3-RPN4
0.2041.01E-56SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0909091TSC3-RPN4
0.2036.40E-56SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.09375TSC3-RPN4
0.2021.85E-55SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.121951TSC3-RPN4
0.1942.88E-51SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.138462TSC3-RPN4
0.1932.13E-50SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.107692TSC3-RPN4
0.1903.41E-49SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0666667TSC3-RPN4
0.1864.78E-47SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.144737
0.1818.48E-45SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.111111TSC3-RPN4
0.1782.35E-43SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0428571TSC3-RPN4
0.1763.17E-42SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0895522TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2592piperlongumine83.33 μM1637858Microsource (Natural product library)317.336462.13505TSC3-RPN4
SGTC_12560570-0116285 μM0.296296410638ChemDiv (Drug-like library)280.362661.81105
SGTC_1925st05777010.1 μM0.2950825989122TimTec (Natural product derivative library)284.306543.68724TSC3-RPN4
SGTC_2586curcumin80 μM0.2878796610332ICCB bioactive library368.37993.55426iron homeostasis
SGTC_444curcumin90.4 μM0.2878796610332ICCB bioactive library368.37993.55426iron homeostasis
SGTC_2902043-672248.1 μM0.269841773758ChemDiv (Drug-like library)281.3241434.02402
SGTC_25992',3-dihydroxy-4,4',6'-trimethoxychalcone100 μM0.2698415379071Microsource (Natural product library)330.331923.16826
SGTC_22537938677135.83 μM0.2678572301972Chembridge (Fragment library)229.660181.66913
SGTC_1565sinapyl alcohol95.1 μM0.2641515280507TimTec (Pure natural product library)210.226461.41824
SGTC_492tmb-8126 μM0.2539685494ICCB bioactive library395.532884.93906
SGTC_3041310-029897 μM0.251550234ChemDiv (Drug-like library)366.407084.6225cell wall