coumarin 106

A hepatotoxic, DNA x-linker.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1600
Screen concentration 71.1 μM
Source TimTec (Pure natural product library)
PubChem CID 100336
SMILES C1CC2=C(C1)C(=O)OC3=C4CCCN5C4=C(CCC5)C=C23
Standardized SMILES O=C1Oc2c3CCCN4CCCc(cc2C5=C1CCC5)c34
Molecular weight 281.349
ALogP 4.1
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.55
% growth inhibition (Hom. pool) 5.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 100336
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC19(YAL038W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.37||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:COG3(YER157W)|FD-Score:4.93|P-value:4.08E-7|Clearance:0.18||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ECM16(YMR128W)|FD-Score:4.75|P-value:1.00E-6|Clearance:1.02||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:MTR3(YGR158C)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:PGK1(YCR012W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.19||SGD DESC:3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis Gene:RNR2(YJL026W)|FD-Score:-3.39|P-value:3.45E-4|Clearance:0||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPB10(YOR210W)|FD-Score:-4.91|P-value:4.60E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPG1(YBR079C)|FD-Score:-3.33|P-value:4.40E-4|Clearance:0||SGD DESC:eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation Gene:SEC13(YLR208W)|FD-Score:8.22|P-value:1.05E-16|Clearance:3.29||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:3.73|P-value:9.60E-5|Clearance:0.11||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:YBR124W(YBR124W_d)|FD-Score:-3.3|P-value:4.86E-4|Clearance:0||SGD DESC:Putative protein of unknown function Gene:CCT3(YJL014W)|FD-Score:-3.61|P-value:1.55E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC19(YAL038W)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.37||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:COG3(YER157W)|FD-Score:4.93|P-value:4.08E-7|Clearance:0.18||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:ECM16(YMR128W)|FD-Score:4.75|P-value:1.00E-6|Clearance:1.02||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:MTR3(YGR158C)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:PGK1(YCR012W)|FD-Score:3.62|P-value:1.49E-4|Clearance:0.19||SGD DESC:3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis Gene:RNR2(YJL026W)|FD-Score:-3.39|P-value:3.45E-4|Clearance:0||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPB10(YOR210W)|FD-Score:-4.91|P-value:4.60E-7|Clearance:0||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPG1(YBR079C)|FD-Score:-3.33|P-value:4.40E-4|Clearance:0||SGD DESC:eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation Gene:SEC13(YLR208W)|FD-Score:8.22|P-value:1.05E-16|Clearance:3.29||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:3.73|P-value:9.60E-5|Clearance:0.11||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:YBR124W(YBR124W_d)|FD-Score:-3.3|P-value:4.86E-4|Clearance:0||SGD DESC:Putative protein of unknown function

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 100336
Download HOP data (tab-delimited text)  (excel)
Gene:ADH3(YMR083W)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:AGE2(YIL044C)|FD-Score:4.59|P-value:2.20E-6||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AIM13(YFR011C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AQY2(YLL052C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARO1(YDR127W)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG14(YBR128C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:CDC26(YFR036W)|FD-Score:4.85|P-value:6.19E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC73(YLR418C)|FD-Score:5.21|P-value:9.24E-8||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CIN4(YMR138W)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CNB1(YKL190W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CNM67(YNL225C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:DJP1(YIR004W)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DSE3(YOR264W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ENV7(YPL236C)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERP1(YAR002C-A)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FAU1(YER183C)|FD-Score:4.86|P-value:5.92E-7||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GAL1(YBR020W)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GCN2(YDR283C)|FD-Score:4.54|P-value:2.81E-6||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:5.51|P-value:1.83E-8||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEF1(YJR040W)|FD-Score:7.84|P-value:2.22E-15||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GLR1(YPL091W)|FD-Score:-3.75|P-value:8.87E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GTB1(YDR221W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:HKR1(YDR420W)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection Gene:HOP2(YGL033W)|FD-Score:-4.31|P-value:8.29E-6||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HSV2(YGR223C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:ISC10(YER180C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:LSM1(YJL124C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LYS1(YIR034C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MSC7(YHR039C)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSI1(YBR195C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:NHP6A(YPR052C)|FD-Score:4.88|P-value:5.27E-7||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:OLA1(YBR025C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:PCI8(YIL071C)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PIN4(YBL051C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POT1(YIL160C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:RCK1(YGL158W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RNH201(YNL072W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPS4B(YHR203C)|FD-Score:-4.74|P-value:1.08E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RRG8(YPR116W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:SGE1(YPR198W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHE2(YKL130C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SPO1(YNL012W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SPO7(YAL009W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSD1(YDR293C)|FD-Score:4.54|P-value:2.87E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TKL1(YPR074C)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:8.38|P-value:2.60E-17||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.03|P-value:4.76E-16||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.76|P-value:6.79E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBC8(YEL012W)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:VFA1(YER128W)|FD-Score:4.79|P-value:8.22E-7||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VMA1(YDL185W)|FD-Score:6.75|P-value:7.21E-12||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS68(YOL129W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YAP1(YML007W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL172C(YDL172C_d)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:4.3|P-value:8.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Putative protein of unknown function Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Putative protein of unknown function Gene:YGR242W(YGR242W_d)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YJL120W(YJL120W_d)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YLR194C(YLR194C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YOR331C(YOR331C_d)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPL225W(YPL225W)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR027C(YPR027C_p)|FD-Score:4.91|P-value:4.46E-7||SGD DESC:Putative protein of unknown function Gene:YSP1(YHR155W)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ZDS1(YMR273C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication Gene:ADH3(YMR083W)|FD-Score:4.02|P-value:2.92E-5||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:AGE2(YIL044C)|FD-Score:4.59|P-value:2.20E-6||SGD DESC:ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif Gene:AIM13(YFR011C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:AQY2(YLL052C)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Water channel that mediates the transport of water across cell membranes, only expressed in proliferating cells, controlled by osmotic signals, may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains Gene:ARO1(YDR127W)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG14(YBR128C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I (with Vps34/15/30p); Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor Gene:CDC26(YFR036W)|FD-Score:4.85|P-value:6.19E-7||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC73(YLR418C)|FD-Score:5.21|P-value:9.24E-8||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CIN4(YMR138W)|FD-Score:-3.3|P-value:4.85E-4||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CNB1(YKL190W)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Calcineurin B; the regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is encoded by CNA1 and/or CMP1; protein abundance increases in response to DNA replication stress Gene:CNM67(YNL225C)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:DJP1(YIR004W)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ Gene:DSE3(YOR264W)|FD-Score:3.61|P-value:1.50E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ENV7(YPL236C)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16) Gene:ERP1(YAR002C-A)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FAU1(YER183C)|FD-Score:4.86|P-value:5.92E-7||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:GAL1(YBR020W)|FD-Score:-3.33|P-value:4.27E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GCN2(YDR283C)|FD-Score:4.54|P-value:2.81E-6||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:5.51|P-value:1.83E-8||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEF1(YJR040W)|FD-Score:7.84|P-value:2.22E-15||SGD DESC:Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism Gene:GLR1(YPL091W)|FD-Score:-3.75|P-value:8.87E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GTB1(YDR221W)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:HKR1(YDR420W)|FD-Score:-3.15|P-value:8.21E-4||SGD DESC:Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection Gene:HOP2(YGL033W)|FD-Score:-4.31|P-value:8.29E-6||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HSV2(YGR223C)|FD-Score:3.49|P-value:2.45E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:ISC10(YER180C)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:LSM1(YJL124C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress Gene:LYS1(YIR034C)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MSC7(YHR039C)|FD-Score:3.14|P-value:8.40E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:MSI1(YBR195C)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:NHP6A(YPR052C)|FD-Score:4.88|P-value:5.27E-7||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:OLA1(YBR025C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress Gene:PCI8(YIL071C)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PIN4(YBL051C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:POT1(YIL160C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:RCK1(YGL158W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Protein kinase involved in the response to oxidative stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication Gene:RNH201(YNL072W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPS4B(YHR203C)|FD-Score:-4.74|P-value:1.08E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RRG8(YPR116W)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Putative protein of unknown function, required for mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport Gene:SGE1(YPR198W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SHE2(YKL130C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud Gene:SPO1(YNL012W)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SPO7(YAL009W)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SSD1(YDR293C)|FD-Score:4.54|P-value:2.87E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:TKL1(YPR074C)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:3.98|P-value:3.43E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:8.38|P-value:2.60E-17||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.03|P-value:4.76E-16||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.76|P-value:6.79E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBC8(YEL012W)|FD-Score:4.42|P-value:4.90E-6||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:VFA1(YER128W)|FD-Score:4.79|P-value:8.22E-7||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VMA1(YDL185W)|FD-Score:6.75|P-value:7.21E-12||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA13(YPR036W)|FD-Score:3.66|P-value:1.29E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS68(YOL129W)|FD-Score:3.91|P-value:4.64E-5||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YAP1(YML007W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YDL172C(YDL172C_d)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:4.3|P-value:8.73E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Putative protein of unknown function Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Putative protein of unknown function Gene:YGR242W(YGR242W_d)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YJL120W(YJL120W_d)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YLR194C(YLR194C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YOR331C(YOR331C_d)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPL225W(YPL225W)|FD-Score:-3.72|P-value:1.00E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPR027C(YPR027C_p)|FD-Score:4.91|P-value:4.46E-7||SGD DESC:Putative protein of unknown function Gene:YSP1(YHR155W)|FD-Score:4.55|P-value:2.64E-6||SGD DESC:Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication Gene:ZDS1(YMR273C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W8.221.05E-163.29SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YER157W4.934.08E-70.18COG3Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YMR128W4.751.00E-61.02ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YDL195W3.739.60E-50.11SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YCR012W3.621.49E-40.19PGK13-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis
YAL038W3.433.03E-40.37CDC19Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
YNL256W3.060.001120.10FOL1Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
YJR065C2.960.001560.05ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YOL123W2.910.001830.06HRP1Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences
YOL077C2.850.002210.07BRX1Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
YDR468C2.780.002750.05TLG1Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
YGR116W2.720.003220.04SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YPR161C2.690.003610.02SGV1Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins
YOR282W_d2.670.003830.05YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
YPL075W2.620.004390.01GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER090W8.382.60E-17TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YKL211C8.034.76E-16TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YJR040W7.842.22E-15GEF1Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
YDR354W6.766.79E-12TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDL185W6.757.21E-12VMA1Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
YKR026C5.511.83E-8GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YLR418C5.219.24E-8CDC73Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
YPR027C_p4.914.46E-7YPR027C_pPutative protein of unknown function
YPR052C4.885.27E-7NHP6AHigh-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YER183C4.865.92E-7FAU15,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis
YFR036W4.856.19E-7CDC26Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YER128W4.798.22E-7VFA1Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology
YML007W4.701.29E-6YAP1Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YIR004W4.631.80E-6DJP1Cytosolic J-domain-containing protein, required for peroxisomal protein import and involved in peroxisome assembly, homologous to E. coli DnaJ
YIL044C4.592.20E-6AGE2ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif

GO enrichment analysis for SGTC_1600
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3411.25E-160SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0869565
0.3358.47E-155SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0714286
0.3336.46E-153SGTC_24885268135 45.7 μMMiscellaneous12703560.0615385
0.3295.86E-149SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0172414
0.3197.11E-140SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0869565
0.3154.36E-136SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.107692
0.3155.48E-136SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0875
0.3151.13E-135SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0493827
0.3147.84E-135SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.0632911
0.3111.79E-132SGTC_24785763493 32.3 μMMiscellaneous13655760.0571429
0.3092.18E-130SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0465116
0.3068.34E-128SGTC_10410206-0046 107.0 μMChemDiv (Drug-like library)504940.15873
0.3051.72E-127SGTC_18825615643 20.0 μMMiscellaneous22530750.0740741TRP & mitochondrial translation
0.3011.34E-123SGTC_18815652484 16.0 μMMiscellaneous22551290.075TRP & mitochondrial translation
0.2993.05E-122SGTC_7234182-0001 115.0 μMChemDiv (Drug-like library)7448210.126984

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1936st07440774.3 μM0.54809813TimTec (Natural product derivative library)269.29522.73504DNA damage response
SGTC_1559coumarin 34370.1 μM0.519231108770TimTec (Pure natural product library)285.29462.71315
SGTC_21425622915118.52 μM0.3673475322196Chembridge (Fragment library)202.2062.63513
SGTC_13973532-019020.1 μM0.3529415322280ChemDiv (Drug-like library)230.259163.57713
SGTC_1977st07072065.3 μM0.3682188TimTec (Natural product derivative library)306.312064.30904
SGTC_2139557869256.47 μM0.210526650991Chembridge (Fragment library)207.248962.1904superoxide
SGTC_23879077560195.08 μM0.25303272Chembridge (Fragment library)260.33481.43713
SGTC_2715st07950056.4 μM0.19718328246688TimTec (Natural product derivative library)354.422822.92314
SGTC_22607964312200 μM0.196429231951Chembridge (Fragment library)213.2753.13502
SGTC_1783st03945987.52 μM0.192982729761TimTec (Natural product derivative library)185.221841.97901Golgi