st000094

2-(cyclohexylamino)-1-[2-(trifluoromethyl)phenothiazin-10-yl]ethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1606
Screen concentration 49.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 1242210
SMILES C1CCC(CC1)NCC(=O)N2C3=CC=CC=C3SC4=C2C=C(C=C4)C(F)(F)F
Standardized SMILES FC(F)(F)c1ccc2Sc3ccccc3N(C(=O)CNC4CCCCC4)c2c1
Molecular weight 406.4645
ALogP 5.29
H-bond donor count 1
H-bond acceptor count 6
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.77
% growth inhibition (Hom. pool) 5.48


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1242210
Download HIP data (tab-delimited text)  (excel)
Gene:CDC24(YAL041W)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.11||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CLP1(YOR250C)|FD-Score:3.96|P-value:3.73E-5|Clearance:0.01||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:COG2(YGR120C)|FD-Score:-4.01|P-value:3.07E-5|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DUO1(YGL061C)|FD-Score:3.15|P-value:8.22E-4|Clearance:0.1||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ECM16(YMR128W)|FD-Score:4.7|P-value:1.29E-6|Clearance:0.37||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:HIP1(YGR191W)|FD-Score:-3.26|P-value:5.54E-4|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:IMP3(YHR148W)|FD-Score:-4.28|P-value:9.38E-6|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:LSG1(YGL099W)|FD-Score:4.33|P-value:7.33E-6|Clearance:0.37||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MTR4(YJL050W)|FD-Score:4.57|P-value:2.40E-6|Clearance:0.37||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NCB2(YDR397C)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.11||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta Gene:NHP2(YDL208W)|FD-Score:-3.54|P-value:1.97E-4|Clearance:0||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:NMD3(YHR170W)|FD-Score:4.78|P-value:8.80E-7|Clearance:0.37||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:ORC6(YHR118C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.12||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:RTT105(YER104W)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.05||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC65(YML105C)|FD-Score:3.29|P-value:5.09E-4|Clearance:0.08||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SMC3(YJL074C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.06||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:SPP41(YDR464W)|FD-Score:-3.22|P-value:6.51E-4|Clearance:0||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:SRP1(YNL189W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.04||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:THG1(YGR024C)|FD-Score:-3.28|P-value:5.11E-4|Clearance:0||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TOM20(YGR082W)|FD-Score:4.33|P-value:7.56E-6|Clearance:0.37||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins Gene:UTP23(YOR004W)|FD-Score:3.95|P-value:3.84E-5|Clearance:0.24||SGD DESC:Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein Gene:CDC24(YAL041W)|FD-Score:3.44|P-value:2.93E-4|Clearance:0.11||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:CLP1(YOR250C)|FD-Score:3.96|P-value:3.73E-5|Clearance:0.01||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:COG2(YGR120C)|FD-Score:-4.01|P-value:3.07E-5|Clearance:0||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DUO1(YGL061C)|FD-Score:3.15|P-value:8.22E-4|Clearance:0.1||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ECM16(YMR128W)|FD-Score:4.7|P-value:1.29E-6|Clearance:0.37||SGD DESC:Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis Gene:HIP1(YGR191W)|FD-Score:-3.26|P-value:5.54E-4|Clearance:0||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:IMP3(YHR148W)|FD-Score:-4.28|P-value:9.38E-6|Clearance:0||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:LSG1(YGL099W)|FD-Score:4.33|P-value:7.33E-6|Clearance:0.37||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MTR4(YJL050W)|FD-Score:4.57|P-value:2.40E-6|Clearance:0.37||SGD DESC:ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing Gene:NCB2(YDR397C)|FD-Score:3.55|P-value:1.91E-4|Clearance:0.11||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta Gene:NHP2(YDL208W)|FD-Score:-3.54|P-value:1.97E-4|Clearance:0||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:NMD3(YHR170W)|FD-Score:4.78|P-value:8.80E-7|Clearance:0.37||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:ORC6(YHR118C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.12||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:RTT105(YER104W)|FD-Score:3.33|P-value:4.31E-4|Clearance:0.05||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SEC65(YML105C)|FD-Score:3.29|P-value:5.09E-4|Clearance:0.08||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SMC3(YJL074C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.06||SGD DESC:Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member Gene:SPP41(YDR464W)|FD-Score:-3.22|P-value:6.51E-4|Clearance:0||SGD DESC:Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3 Gene:SRP1(YNL189W)|FD-Score:3.71|P-value:1.02E-4|Clearance:0.04||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:THG1(YGR024C)|FD-Score:-3.28|P-value:5.11E-4|Clearance:0||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TOM20(YGR082W)|FD-Score:4.33|P-value:7.56E-6|Clearance:0.37||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins Gene:UTP23(YOR004W)|FD-Score:3.95|P-value:3.84E-5|Clearance:0.24||SGD DESC:Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1242210
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP8(YOR141C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG8(YBL078C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BTS1(YPL069C)|FD-Score:-4.27|P-value:9.66E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUR2(YLR226W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II Gene:CLN1(YMR199W)|FD-Score:4.39|P-value:5.72E-6||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:DOC1(YGL240W)|FD-Score:4.77|P-value:9.06E-7||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DYN2(YDR424C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:DYN3(YMR299C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EAF6(YJR082C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ERP4(YOR016C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:ERS1(YCR075C)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:FIR1(YER032W)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FMP27(YLR454W_p)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN4(YEL009C)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GTR2(YGR163W)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUT1(YHL032C)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HDA2(YDR295C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:IRC13(YOR235W_d)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:JEM1(YJL073W)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:MEI5(YPL121C)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p Gene:MRN1(YPL184C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRP13(YGR084C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL20(YKR085C)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NMA1(YLR328W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:NPL3(YDR432W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NUR1(YDL089W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PAN3(YKL025C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDR16(YNL231C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX13(YLR191W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PTR2(YKR093W)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RGP1(YDR137W)|FD-Score:-4.28|P-value:9.31E-6||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RKM3(YBR030W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RKM4(YDR257C)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RPL21A(YBR191W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAP4(YGL229C)|FD-Score:-5.9|P-value:1.83E-9||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SBE22(YHR103W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SET6(YPL165C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SGO1(YOR073W)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SSB2(YNL209W)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication Gene:STF1(YDL130W-A)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SWA2(YDR320C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWM2(YNR004W_p)|FD-Score:-3.76|P-value:8.55E-5||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TCB2(YNL087W)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TDH1(YJL052W)|FD-Score:-4.48|P-value:3.73E-6||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:THI72(YOR192C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TOS3(YGL179C)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:TPN1(YGL186C)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:URK1(YNR012W)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP Gene:VAC14(YLR386W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS45(YGL095C)|FD-Score:4.37|P-value:6.21E-6||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS51(YKR020W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL066W(YAL066W_d)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAR029W(YAR029W_p)|FD-Score:6.39|P-value:8.47E-11||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YCR015C(YCR015C_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCT1(YLL055W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YER158C(YER158C_p)|FD-Score:4.29|P-value:8.93E-6||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YGR066C(YGR066C_p)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Putative protein of unknown function Gene:YHR182W(YHR182W_p)|FD-Score:-4.88|P-value:5.25E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YKL023W(YKL023W_p)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL044W(YKL044W_p)|FD-Score:4.99|P-value:2.96E-7||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YNL285W(YNL285W_d)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR277C(YOR277C_d)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPL041C(YPL041C_p)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YSC83(YHR017W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:AFT1(YGL071W)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP8(YOR141C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes; has mRNA binding activity Gene:ATG8(YBL078C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BTS1(YPL069C)|FD-Score:-4.27|P-value:9.66E-6||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUR2(YLR226W)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II Gene:CLN1(YMR199W)|FD-Score:4.39|P-value:5.72E-6||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:DOC1(YGL240W)|FD-Score:4.77|P-value:9.06E-7||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DYN2(YDR424C)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Cytoplasmic light chain dynein, microtubule motor protein; proposed to be involved in the assembly of the nuclear pore complex Gene:DYN3(YMR299C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EAF6(YJR082C)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ERP4(YOR016C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:ERS1(YCR075C)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:FIR1(YER032W)|FD-Score:3.1|P-value:9.83E-4||SGD DESC:Protein involved in 3' mRNA processing, interacts with Ref2p; potential Cdc28p substrate Gene:FMP27(YLR454W_p)|FD-Score:3.3|P-value:4.85E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN4(YEL009C)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GTR2(YGR163W)|FD-Score:-4.13|P-value:1.85E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUT1(YHL032C)|FD-Score:3.76|P-value:8.38E-5||SGD DESC:Glycerol kinase, converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p Gene:HDA2(YDR295C)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex containing an Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance Gene:IRC13(YOR235W_d)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:JEM1(YJL073W)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:MEI5(YPL121C)|FD-Score:4.03|P-value:2.77E-5||SGD DESC:Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p Gene:MRN1(YPL184C)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:MRP13(YGR084C)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL20(YKR085C)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NMA1(YLR328W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication Gene:NPL3(YDR432W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:NUR1(YDL089W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:PAN3(YKL025C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDR16(YNL231C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PEX13(YLR191W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PTR2(YKR093W)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RGP1(YDR137W)|FD-Score:-4.28|P-value:9.31E-6||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RKM3(YBR030W)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein Gene:RKM4(YDR257C)|FD-Score:3.84|P-value:6.23E-5||SGD DESC:Ribosomal lysine methyltransferase specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein Gene:RPL21A(YBR191W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAP4(YGL229C)|FD-Score:-5.9|P-value:1.83E-9||SGD DESC:Protein required for function of the Sit4p protein phosphatase, member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p Gene:SBE22(YHR103W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SET6(YPL165C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability Gene:SGO1(YOR073W)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SSB2(YNL209W)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication Gene:STF1(YDL130W-A)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SWA2(YDR320C)|FD-Score:-3.38|P-value:3.68E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWM2(YNR004W_p)|FD-Score:-3.76|P-value:8.55E-5||SGD DESC:Putative protein of unknown function; haploid disruptant exhibits cold-sensitive growth and elongated buds Gene:TCB2(YNL087W)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud Gene:TDH1(YJL052W)|FD-Score:-4.48|P-value:3.73E-6||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:THI72(YOR192C)|FD-Score:3.84|P-value:6.10E-5||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TOS3(YGL179C)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:TPN1(YGL186C)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:URK1(YNR012W)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP Gene:VAC14(YLR386W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS45(YGL095C)|FD-Score:4.37|P-value:6.21E-6||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS51(YKR020W)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:VPS61(YDR136C_d)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL066W(YAL066W_d)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAR029W(YAR029W_p)|FD-Score:6.39|P-value:8.47E-11||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YCR015C(YCR015C_p)|FD-Score:3.14|P-value:8.44E-4||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCT1(YLL055W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YER158C(YER158C_p)|FD-Score:4.29|P-value:8.93E-6||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YGR066C(YGR066C_p)|FD-Score:-3.09|P-value:9.89E-4||SGD DESC:Putative protein of unknown function Gene:YHR182W(YHR182W_p)|FD-Score:-4.88|P-value:5.25E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:YKL023W(YKL023W_p)|FD-Score:3.33|P-value:4.35E-4||SGD DESC:Putative protein of unknown function, predicted by computational methods to be involved in mRNA degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YKL044W(YKL044W_p)|FD-Score:4.99|P-value:2.96E-7||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YNL285W(YNL285W_d)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.33|P-value:4.29E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR277C(YOR277C_d)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YPL041C(YPL041C_p)|FD-Score:-3.34|P-value:4.24E-4||SGD DESC:Protein of unknown function involved in maintenance of proper telomere length Gene:YSC83(YHR017W)|FD-Score:3.16|P-value:7.82E-4||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR170W4.788.80E-70.36NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YMR128W4.701.29E-60.36ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YJL050W4.572.40E-60.36MTR4ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
YGL099W4.337.33E-60.36LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YGR082W4.337.56E-60.36TOM20Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
YOR250C3.963.73E-50.01CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YOR004W3.953.84E-50.24UTP23Component of the small subunit processome, involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein
YNL189W3.711.02E-40.04SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YHR118C3.681.18E-40.12ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YDR397C3.551.91E-40.11NCB2Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta
YAL041W3.442.93E-40.11CDC24Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress
YER104W3.334.31E-40.05RTT105Protein with a role in regulation of Ty1 transposition
YML105C3.295.09E-40.08SEC65Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
YJL074C3.216.71E-40.06SMC3Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
YGL061C3.158.22E-40.10DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YAR029W_p6.398.47E-11YAR029W_pMember of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YGL071W5.462.38E-8AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YGL186C5.091.80E-7TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YKL044W_p4.992.96E-7YKL044W_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YGL240W4.779.06E-7DOC1Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain
YJL073W4.671.51E-6JEM1DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
YKR093W4.493.48E-6PTR2Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p
YMR199W4.395.72E-6CLN1G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
YGL095C4.376.21E-6VPS45Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
YER158C_p4.298.93E-6YER158C_pProtein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication
YDL130W-A4.221.21E-5STF1Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress
YHR103W4.201.32E-5SBE22Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth
YCR075C4.072.32E-5ERS1Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains
YGL179C4.062.47E-5TOS3Protein kinase, related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome
YPL121C4.032.77E-5MEI5Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p

GO enrichment analysis for SGTC_1606
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1102.42E-17SGTC_20785212016 200.0 μMChembridge (Fragment library)18997080.08450760S ribosome export
0.1081.08E-16SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.12328860S ribosome export
0.1031.55E-15SGTC_9342950-0684 338.0 μMChemDiv (Drug-like library)11212530.101124
0.0961.69E-13SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.080808160S ribosome export
0.0951.99E-13SGTC_32189131285 49.5 μMChembridge (Drug-like library)171564170.0886076endomembrane recycling
0.0938.98E-13SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.060975660S ribosome export
0.0912.22E-12SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.09756160S ribosome export
0.0903.48E-12SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.137560S ribosome export
0.0898.12E-12SGTC_7064262-0057 46.9 μMChemDiv (Drug-like library)8832980.084507fatty acid desaturase (OLE1)
0.0871.87E-11SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.075949460S ribosome export
0.0822.82E-10SGTC_31009120536 49.5 μMChembridge (Drug-like library)164211960.0689655
0.0814.00E-10SGTC_1071trifluoperazine 6.3 μMNIH Clinical Collection29135350.402985
0.0815.84E-10SGTC_9541189-0853 2.9 μMChemDiv (Drug-like library)28281940.08endomembrane recycling
0.0809.38E-10SGTC_1081sertraline 6.0 μMNIH Clinical Collection630090.13698660S ribosome export
0.0791.31E-9SGTC_1775st081588 35.2 μMTimTec (Natural product derivative library)14264430.07608760S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1605st00008249.1 μM0.573771114022TimTec (Natural product derivative library)407.452513.9270760S ribosome export
SGTC_430570-010572.5 μM0.43751114046ChemDiv (Drug-like library)380.890484.73913ERG2
SGTC_221trifluoperazine10.3 μM0.4029855566Miscellaneous407.495574.97507NEO1-PIK1
SGTC_1093fluphenazine7.97 μM0.4029856602611NIH Clinical Collection473.982494.81728
SGTC_2729fluphenazine10.66 μM0.4029853372Miscellaneous437.521554.43518
SGTC_1062mesoridazine14.2 μM0.3026324078Miscellaneous386.573944.45105
SGTC_3172910586349.47 μM0.2972972348785Chembridge (Drug-like library)389.414093.80806ERG2
SGTC_2773thioridazine9.09 μM0.2933335452Miscellaneous370.574545.56304
SGTC_13381486-122376 μM0.2716053444526ChemDiv (Drug-like library)483.503875.3727
SGTC_1139k015-002446.9 μM0.2647062766652ChemDiv (Drug-like library)305.251313.40205PDR1
SGTC_2831799903619.48 μM0.2638892988270Chembridge (Drug-like library)323.309654.03115