st001799

ethyl 5-acetyloxy-4-(benzenesulfonyl)-1,2-dimethylindole-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1610
Screen concentration 48.1 μM
Source TimTec (Natural product derivative library)
PubChem CID 621897
SMILES CCOC(=O)C1=C(N(C2=C1C(=C(C=C2)OC(=O)C)S(=O)(=O)C3=CC=CC=C3)C)C
Standardized SMILES CCOC(=O)c1c(C)n(C)c2ccc(OC(=O)C)c(c12)S(=O)(=O)c3ccccc3
Molecular weight 415.4595
ALogP 3.55
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.14
% growth inhibition (Hom. pool) 1.14


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 621897
Download HIP data (tab-delimited text)  (excel)
Gene:ATM1(YMR301C)|FD-Score:-3.75|P-value:8.70E-5|Clearance:0||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:FCP1(YMR277W)|FD-Score:3.1|P-value:9.62E-4|Clearance:0.4||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:PRE2(YPR103W)|FD-Score:-3.95|P-value:3.84E-5|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RPP1(YHR062C)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.25||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TFA2(YKR062W)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.15||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:UTP22(YGR090W)|FD-Score:5.46|P-value:2.32E-8|Clearance:1.76||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YNL247W(YNL247W)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.21||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:ATM1(YMR301C)|FD-Score:-3.75|P-value:8.70E-5|Clearance:0||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:FCP1(YMR277W)|FD-Score:3.1|P-value:9.62E-4|Clearance:0.4||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:PRE2(YPR103W)|FD-Score:-3.95|P-value:3.84E-5|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RPP1(YHR062C)|FD-Score:3.71|P-value:1.04E-4|Clearance:0.25||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:TFA2(YKR062W)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.15||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:UTP22(YGR090W)|FD-Score:5.46|P-value:2.32E-8|Clearance:1.76||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YNL247W(YNL247W)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.21||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 621897
Download HOP data (tab-delimited text)  (excel)
Gene:AHC1(YOR023C)|FD-Score:-3.32|P-value:4.42E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:AHP1(YLR109W)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:ATG29(YPL166W)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATP19(YOL077W-A)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:CCW14(YLR390W-A)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall Gene:CDC10(YCR002C)|FD-Score:4.88|P-value:5.40E-7||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CIT2(YCR005C)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication Gene:CMC4(YMR194C-B)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CUE3(YGL110C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination Gene:CWC27(YPL064C)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:DUS4(YLR405W)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ELP6(YMR312W)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FAR11(YNL127W)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FEN1(YCR034W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FLD1(YLR404W)|FD-Score:-5.86|P-value:2.36E-9||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FRT1(YOR324C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GAS2(YLR343W)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:HAP3(YBL021C)|FD-Score:-3.94|P-value:4.05E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HNT1(YDL125C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HNT3(YOR258W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:IES2(YNL215W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IES6(YEL044W)|FD-Score:4.9|P-value:4.75E-7||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:ILV6(YCL009C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IRS4(YKR019C)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISY1(YJR050W)|FD-Score:4.38|P-value:5.89E-6||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:LTP1(YPR073C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MAD1(YGL086W)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Coiled-coil protein involved in the spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p Gene:MAL32(YBR299W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MDV1(YJL112W)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MSD1(YPL104W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSE1(YOL033W)|FD-Score:4.98|P-value:3.21E-7||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:NMD4(YLR363C)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NQM1(YGR043C)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:NUP84(YDL116W)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:OAF1(YAL051W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:PAC10(YGR078C)|FD-Score:7.41|P-value:6.26E-14||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAN2(YGL094C)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PFA5(YDR459C)|FD-Score:-3.74|P-value:9.35E-5||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PHA2(YNL316C)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PML39(YML107C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:POC4(YPL144W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PPE1(YHR075C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PTK1(YKL198C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:PUT3(YKL015W)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:QCR6(YFR033C)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RCE1(YMR274C)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RIC1(YLR039C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPS1B(YML063W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RTR1(YER139C)|FD-Score:4.84|P-value:6.63E-7||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SKN7(YHR206W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLC1(YDL052C)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SSK1(YLR006C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SSP120(YLR250W)|FD-Score:-4.91|P-value:4.58E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SUC2(YIL162W)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:TDA6(YPR157W_p)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:THI74(YDR438W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TPP1(YMR156C)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRX3(YCR083W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:UPS3(YDR185C)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:YAP1(YML007W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YAR035C-A(YAR035C-A_p)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBL070C(YBL070C_d)|FD-Score:-4.21|P-value:1.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL086C(YBL086C_p)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YHI9(YHR029C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YJL211C(YJL211C_d)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YJR151W-A(YJR151W-A_p)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKE4(YIL023C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL069W(YKL069W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKR018C(YKR018C_p)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR252W(YLR252W_d)|FD-Score:-4.43|P-value:4.70E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR257W(YLR257W_p)|FD-Score:-4.62|P-value:1.88E-6||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YLR279W(YLR279W_d)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR311C(YLR311C_d)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR455W(YLR455W_p)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YME1(YPR024W)|FD-Score:-4.27|P-value:9.73E-6||SGD DESC:Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover Gene:YPL229W(YPL229W_p)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:AHC1(YOR023C)|FD-Score:-3.32|P-value:4.42E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:AHP1(YLR109W)|FD-Score:3.76|P-value:8.52E-5||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:ATG29(YPL166W)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATP19(YOL077W-A)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Subunit k of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase Gene:CCW14(YLR390W-A)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall Gene:CDC10(YCR002C)|FD-Score:4.88|P-value:5.40E-7||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CIT2(YCR005C)|FD-Score:-4.01|P-value:3.08E-5||SGD DESC:Citrate synthase; catalyzes the condensation of acetyl coenzyme A and oxaloacetate to form citrate, peroxisomal isozyme involved in glyoxylate cycle; expression is controlled by Rtg1p and Rtg2p transcription factors; CIT2 has a paralog, CIT1, that arose from the whole genome duplication Gene:CMC4(YMR194C-B)|FD-Score:-3.6|P-value:1.61E-4||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CUE3(YGL110C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination Gene:CWC27(YPL064C)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:DUS4(YLR405W)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ELP6(YMR312W)|FD-Score:3.13|P-value:8.87E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:FAR11(YNL127W)|FD-Score:3.14|P-value:8.36E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FEN1(YCR034W)|FD-Score:3.14|P-value:8.32E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FLD1(YLR404W)|FD-Score:-5.86|P-value:2.36E-9||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FRT1(YOR324C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication Gene:GAS2(YLR343W)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:1,3-beta-glucanosyltransferase, involved with Gas4p in spore wall assembly; has similarity to Gas1p Gene:HAP3(YBL021C)|FD-Score:-3.94|P-value:4.05E-5||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HNT1(YDL125C)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins and similar to Hint; protein abundance increases in response to DNA replication stress Gene:HNT3(YOR258W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress Gene:IES2(YNL215W)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress Gene:IES6(YEL044W)|FD-Score:4.9|P-value:4.75E-7||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes Gene:ILV6(YCL009C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IRS4(YKR019C)|FD-Score:-3.21|P-value:6.70E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISY1(YJR050W)|FD-Score:4.38|P-value:5.89E-6||SGD DESC:Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles Gene:LTP1(YPR073C)|FD-Score:-3.23|P-value:6.25E-4||SGD DESC:Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine Gene:MAD1(YGL086W)|FD-Score:-3.17|P-value:7.59E-4||SGD DESC:Coiled-coil protein involved in the spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p Gene:MAL32(YBR299W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose Gene:MDV1(YJL112W)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:MSD1(YPL104W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSE1(YOL033W)|FD-Score:4.98|P-value:3.21E-7||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:NMD4(YLR363C)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NQM1(YGR043C)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift Gene:NUP84(YDL116W)|FD-Score:3.75|P-value:8.76E-5||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107 Gene:OAF1(YAL051W)|FD-Score:3.68|P-value:1.16E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:PAC10(YGR078C)|FD-Score:7.41|P-value:6.26E-14||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PAN2(YGL094C)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PFA5(YDR459C)|FD-Score:-3.74|P-value:9.35E-5||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PHA2(YNL316C)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PML39(YML107C)|FD-Score:4.21|P-value:1.26E-5||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:POC4(YPL144W)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PPE1(YHR075C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Protein with carboxyl methyl esterase activity that may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein Gene:PTK1(YKL198C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein Gene:PUT3(YKL015W)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain Gene:QCR6(YFR033C)|FD-Score:3.12|P-value:9.05E-4||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RCE1(YMR274C)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RIC1(YLR039C)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPS1B(YML063W)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Ribosomal protein 10 (rp10) of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication Gene:RTR1(YER139C)|FD-Score:4.84|P-value:6.63E-7||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SKN7(YHR206W)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SLC1(YDL052C)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes Gene:SSK1(YLR006C)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SSP120(YLR250W)|FD-Score:-4.91|P-value:4.58E-7||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SUC2(YIL162W)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively Gene:TDA6(YPR157W_p)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:THI74(YDR438W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TPP1(YMR156C)|FD-Score:3.32|P-value:4.55E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRX3(YCR083W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:UPS3(YDR185C)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:YAP1(YML007W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YAR035C-A(YAR035C-A_p)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBL070C(YBL070C_d)|FD-Score:-4.21|P-value:1.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL086C(YBL086C_p)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YHI9(YHR029C)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YJL211C(YJL211C_d)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YJR151W-A(YJR151W-A_p)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational guilt by association analysis Gene:YKE4(YIL023C)|FD-Score:3.55|P-value:1.89E-4||SGD DESC:Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family Gene:YKL069W(YKL069W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress Gene:YKR018C(YKR018C_p)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLR252W(YLR252W_d)|FD-Score:-4.43|P-value:4.70E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism Gene:YLR257W(YLR257W_p)|FD-Score:-4.62|P-value:1.88E-6||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YLR279W(YLR279W_d)|FD-Score:3.14|P-value:8.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR311C(YLR311C_d)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR455W(YLR455W_p)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YME1(YPR024W)|FD-Score:-4.27|P-value:9.73E-6||SGD DESC:Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover Gene:YPL229W(YPL229W_p)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YGR090W5.462.32E-81.76UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YHR062C3.711.04E-40.25RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YKR062W3.462.68E-40.15TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YNL247W3.314.71E-40.21YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YMR277W3.109.62E-40.40FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YLR208W2.700.003470.01SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YLR359W2.680.003630.07ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YDR413C_d2.620.004467.21E-4YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing
YDR228C2.610.004470.02PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YPL075W2.590.004740.02GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
YHL015W2.570.005040.01RPS20Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
YKL186C2.570.005150.01MTR2mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
YOR146W_d2.550.005370.00YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YHR101C2.550.005440.01BIG1Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
YPL016W2.540.005620.01SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YGR078C7.416.26E-14PAC10Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding
YOL033W4.983.21E-7MSE1Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated
YEL044W4.904.75E-7IES6Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes
YCR002C4.885.40E-7CDC10Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress
YER139C4.846.63E-7RTR1CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication
YPL064C4.671.54E-6CWC27Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
YJR050W4.385.89E-6ISY1Member of NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs, interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
YPL166W4.251.07E-5ATG29Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YML107C4.211.26E-5PML39Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes
YML007W4.171.52E-5YAP1Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YDR185C4.102.07E-5UPS3Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations
YIL162W4.013.01E-5SUC2Invertase, sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively
YLR109W3.768.52E-5AHP1Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p
YDL116W3.758.76E-5NUP84Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107
YMR274C3.739.74E-5RCE1Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone

GO enrichment analysis for SGTC_1610
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0764.35E-9SGTC_2681menadione 3.2 μMMiscellaneous40550.126984superoxide
0.0759.91E-9SGTC_31409094243 49.5 μMChembridge (Drug-like library)192911120.144578
0.0714.85E-8SGTC_30099080819 71.4 μMChembridge (Drug-like library)170272160.0963855
0.0691.00E-7SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465410.101124RNA pol III & RNase P/MRP
0.0612.96E-6SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.1superoxide
0.0613.07E-6SGTC_28609025725 39.0 μMChembridge (Drug-like library)405462480.119048RPP1 & pyrimidine depletion
0.0586.96E-6SGTC_1653st012112 65.0 μMTimTec (Natural product derivative library)7204290.157895
0.0588.44E-6SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.101124RPP1 & pyrimidine depletion
0.0571.01E-5SGTC_15346-azauridine 81.6 μMTimTec (Pure natural product library)2335020.0487805RNA pol III & RNase P/MRP
0.0571.01E-5SGTC_32709137563 49.5 μMChembridge (Drug-like library)170157240.155844RPP1 & pyrimidine depletion
0.0571.35E-5SGTC_1607st000305 50.6 μMTimTec (Natural product derivative library)54576900.11236
0.0561.67E-5SGTC_1576erythromycin ethylsuccinate 23.2 μMTimTec (Pure natural product library)32560.0866142
0.0561.86E-5SGTC_1136pergolide 1.1 μMTimTec (Natural product library)478110.0869565
0.0527.24E-5SGTC_6164092-0450 108.0 μMChemDiv (Drug-like library)7871650.164384
0.0518.20E-5SGTC_14214029-0249 71.4 μMChemDiv (Drug-like library)28940950.115385

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_530973-002339.98 μM0.323077674768ChemDiv (Drug-like library)261.33943.3714RNA processing & uracil transport
SGTC_397arbidol50 μM0.304878131410Miscellaneous531.890725.49136cell wall
SGTC_2874904390471.43 μM0.2985074463428Chembridge (Drug-like library)312.38460.9606
SGTC_1612st00223345.4 μM0.2948721035021TimTec (Natural product derivative library)431.322924.99304
SGTC_1779st03942755 μM0.2763161124498TimTec (Natural product derivative library)363.45283.50103
SGTC_2626ethyl orsellinate100 μM0.27419475653Microsource (Natural product library)196.199882.03624
SGTC_13923455-103733.9 μM0.265823981132ChemDiv (Drug-like library)396.479424.05216
SGTC_1030844-010451.84 μM0.2656255375804ChemDiv (Drug-like library)206.241062.10514ERAD & cell cycle
SGTC_3730844-010425.9 μM0.2656255375804ChemDiv (Drug-like library)206.241062.10514ERAD & cell cycle
SGTC_2113455-0679143.5 μM0.264706748649ChemDiv (Drug-like library)220.221282.87314
SGTC_14843473-2379286 μM0.263889671917ChemDiv (Drug-like library)279.311621.89825