st001856

2-methyl-[1]benzofuro[3,2-d][1,3]oxazin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1611
Screen concentration 99.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 647478
SMILES CC1=NC2=C(C(=O)O1)OC3=CC=CC=C32
Standardized SMILES CC1=Nc2c(oc3ccccc23)C(=O)O1
Molecular weight 201.1782
ALogP 2.07
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.07
% growth inhibition (Hom. pool) -1.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 647478
Download HIP data (tab-delimited text)  (excel)
Gene:AAR2(YBL074C)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.12||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:ABF1(YKL112W)|FD-Score:3.49|P-value:2.45E-4|Clearance:0.01||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CDC53(YDL132W)|FD-Score:-4.64|P-value:1.70E-6|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:DIS3(YOL021C)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.05||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ERG10(YPL028W)|FD-Score:3.95|P-value:3.83E-5|Clearance:0.05||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:EXO70(YJL085W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.03||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FCP1(YMR277W)|FD-Score:3.81|P-value:7.02E-5|Clearance:0.01||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:HRR25(YPL204W)|FD-Score:3.8|P-value:7.30E-5|Clearance:0.14||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HSF1(YGL073W)|FD-Score:3.28|P-value:5.18E-4|Clearance:0||SGD DESC:Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated Gene:HSH49(YOR319W)|FD-Score:4.87|P-value:5.45E-7|Clearance:0.54||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LAS1(YKR063C)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.03||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:MTW1(YAL034W-A)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.08||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NAN1(YPL126W)|FD-Score:3.17|P-value:7.74E-4|Clearance:0.25||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:NUG1(YER006W)|FD-Score:3.28|P-value:5.23E-4|Clearance:0.03||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PMA1(YGL008C)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.04||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:PRE6(YOL038W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.18||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PTI1(YGR156W)|FD-Score:4.34|P-value:7.26E-6|Clearance:0.15||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:USB1(YLR132C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.15||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants Gene:UTP22(YGR090W)|FD-Score:3.9|P-value:4.79E-5|Clearance:0.01||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YAH1(YPL252C)|FD-Score:3.89|P-value:5.09E-5|Clearance:0.08||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YNL247W(YNL247W)|FD-Score:7.37|P-value:8.60E-14|Clearance:2.49||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:YPL251W(YPL251W_d)|FD-Score:3.48|P-value:2.55E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:AAR2(YBL074C)|FD-Score:3.44|P-value:2.96E-4|Clearance:0.12||SGD DESC:Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron Gene:ABF1(YKL112W)|FD-Score:3.49|P-value:2.45E-4|Clearance:0.01||SGD DESC:DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair Gene:CDC53(YDL132W)|FD-Score:-4.64|P-value:1.70E-6|Clearance:0||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:DIS3(YOL021C)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.05||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ERG10(YPL028W)|FD-Score:3.95|P-value:3.83E-5|Clearance:0.05||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:EXO70(YJL085W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.03||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FCP1(YMR277W)|FD-Score:3.81|P-value:7.02E-5|Clearance:0.01||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:HRR25(YPL204W)|FD-Score:3.8|P-value:7.30E-5|Clearance:0.14||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HSF1(YGL073W)|FD-Score:3.28|P-value:5.18E-4|Clearance:0||SGD DESC:Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated Gene:HSH49(YOR319W)|FD-Score:4.87|P-value:5.45E-7|Clearance:0.54||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:LAS1(YKR063C)|FD-Score:3.66|P-value:1.25E-4|Clearance:0.03||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:MTW1(YAL034W-A)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.08||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly Gene:NAN1(YPL126W)|FD-Score:3.17|P-value:7.74E-4|Clearance:0.25||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:NUG1(YER006W)|FD-Score:3.28|P-value:5.23E-4|Clearance:0.03||SGD DESC:GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus Gene:PMA1(YGL008C)|FD-Score:3.32|P-value:4.52E-4|Clearance:0.04||SGD DESC:Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+] Gene:PRE6(YOL038W)|FD-Score:4.14|P-value:1.76E-5|Clearance:0.18||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PTI1(YGR156W)|FD-Score:4.34|P-value:7.26E-6|Clearance:0.15||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:USB1(YLR132C)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.15||SGD DESC:Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants Gene:UTP22(YGR090W)|FD-Score:3.9|P-value:4.79E-5|Clearance:0.01||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YAH1(YPL252C)|FD-Score:3.89|P-value:5.09E-5|Clearance:0.08||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YNL247W(YNL247W)|FD-Score:7.37|P-value:8.60E-14|Clearance:2.49||SGD DESC:Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments Gene:YPL251W(YPL251W_d)|FD-Score:3.48|P-value:2.55E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 647478
Download HOP data (tab-delimited text)  (excel)
Gene:ADA2(YDR448W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AIM34(YMR003W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss Gene:AIM43(YPL099C)|FD-Score:-3.14|P-value:8.34E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIM45(YPR004C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ARR3(YPR201W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ATG11(YPR049C)|FD-Score:4.45|P-value:4.27E-6||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATG16(YMR159C)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BAG7(YOR134W)|FD-Score:5.26|P-value:7.40E-8||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:DIE2(YGR227W)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DNM1(YLL001W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Gene:DSF2(YBR007C_p)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DYN3(YMR299C)|FD-Score:4.43|P-value:4.77E-6||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EAF1(YDR359C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:ELP6(YMR312W)|FD-Score:7.09|P-value:6.86E-13||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EPS1(YIL005W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ESC2(YDR363W)|FD-Score:6.49|P-value:4.42E-11||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:FLD1(YLR404W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FTR1(YER145C)|FD-Score:5.37|P-value:3.91E-8||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GET3(YDL100C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GFD2(YCL036W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GLC3(YEL011W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GPX2(YBR244W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress Gene:GRX4(YER174C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:GYP6(YJL044C)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HFM1(YGL251C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HPF1(YOL155C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HPT1(YDR399W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:MDM38(YOL027C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MSD1(YPL104W)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MYO4(YAL029C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NTE1(YML059C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:NUC1(YJL208C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:PDC6(YGR087C)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PEP12(YOR036W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PFA5(YDR459C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PXL1(YKR090W)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAD34(YDR314C)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:RLF2(YPR018W)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RMD8(YFR048W)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RTG2(YGL252C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RUD3(YOR216C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAE2(YGL175C)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SAW1(YAL027W)|FD-Score:6.03|P-value:8.46E-10||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SNC1(YAL030W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SWF1(YDR126W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TDA6(YPR157W_p)|FD-Score:4.34|P-value:7.14E-6||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:UPC2(YDR213W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:VPS55(YJR044C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:WHI4(YDL224C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAT1(YAR035W)|FD-Score:4.34|P-value:7.24E-6||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBP2(YGL060W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YCR102C(YCR102C_p)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YFL051C(YFL051C_p)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YFR006W(YFR006W_p)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YHR080C(YHR080C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YHR140W(YHR140W_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YIR018C-A(YIR018C-A_p)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YKL107W(YKL107W_p)|FD-Score:4.31|P-value:8.06E-6||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YLR202C(YLR202C_d)|FD-Score:5.44|P-value:2.68E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR279W(YLR279W_d)|FD-Score:-4.61|P-value:1.99E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML018C(YML018C_p)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL144C(YNL144C_p)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YOL118C(YOL118C_d)|FD-Score:-4.66|P-value:1.57E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR052C(YOR052C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YSC83(YHR017W)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83 Gene:ADA2(YDR448W)|FD-Score:-3.37|P-value:3.75E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AIM34(YMR003W)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss Gene:AIM43(YPL099C)|FD-Score:-3.14|P-value:8.34E-4||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIM45(YPR004C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ARR3(YPR201W)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ATG11(YPR049C)|FD-Score:4.45|P-value:4.27E-6||SGD DESC:Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS) Gene:ATG16(YMR159C)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:BAG7(YOR134W)|FD-Score:5.26|P-value:7.40E-8||SGD DESC:Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p Gene:DIE2(YGR227W)|FD-Score:3.87|P-value:5.41E-5||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DNM1(YLL001W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Gene:DSF2(YBR007C_p)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DYN3(YMR299C)|FD-Score:4.43|P-value:4.77E-6||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:EAF1(YDR359C)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1 Gene:ELP6(YMR312W)|FD-Score:7.09|P-value:6.86E-13||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:EPS1(YIL005W)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ESC2(YDR363W)|FD-Score:6.49|P-value:4.42E-11||SGD DESC:Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member Gene:FLD1(YLR404W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FTR1(YER145C)|FD-Score:5.37|P-value:3.91E-8||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GET3(YDL100C)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GFD2(YCL036W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication Gene:GLC3(YEL011W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:GPX2(YBR244W)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress Gene:GRX4(YER174C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:GYP6(YJL044C)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HFM1(YGL251C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity Gene:HPF1(YOL155C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Haze-protective mannoprotein that reduces the particle size of aggregated proteins in white wines Gene:HPT1(YDR399W)|FD-Score:-3.11|P-value:9.45E-4||SGD DESC:Dimeric hypoxanthine-guanine phosphoribosyltransferase, catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome Gene:MDM38(YOL027C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MSD1(YPL104W)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MYO4(YAL029C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication Gene:NTE1(YML059C)|FD-Score:3.29|P-value:5.05E-4||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:NUC1(YJL208C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:PDC6(YGR087C)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PEP12(YOR036W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PFA5(YDR459C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain Gene:PXL1(YKR090W)|FD-Score:3.3|P-value:4.92E-4||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAD34(YDR314C)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Protein involved in nucleotide excision repair (NER); homologous to RAD4 Gene:RLF2(YPR018W)|FD-Score:3.36|P-value:3.97E-4||SGD DESC:Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription Gene:RMD8(YFR048W)|FD-Score:3.14|P-value:8.54E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RTG2(YGL252C)|FD-Score:3.21|P-value:6.59E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RUD3(YOR216C)|FD-Score:3.13|P-value:8.88E-4||SGD DESC:Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 Gene:SAE2(YGL175C)|FD-Score:3.76|P-value:8.33E-5||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SAW1(YAL027W)|FD-Score:6.03|P-value:8.46E-10||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SNC1(YAL030W)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication Gene:SWF1(YDR126W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:TDA6(YPR157W_p)|FD-Score:4.34|P-value:7.14E-6||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:UPC2(YDR213W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Sterol regulatory element binding protein; induces transcription of sterol biosynthetic genes and of DAN/TIR gene products; relocates from intracellular membranes to perinuclear foci on sterol depletion; UPC2 has a paralog, ECM22, that arose from the whole genome duplication Gene:VPS55(YJR044C)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:WHI4(YDL224C)|FD-Score:3.77|P-value:8.30E-5||SGD DESC:Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication Gene:YAL016C-B(YAL016C-B_d)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAT1(YAR035W)|FD-Score:4.34|P-value:7.24E-6||SGD DESC:Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated Gene:YBP2(YGL060W)|FD-Score:4|P-value:3.23E-5||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YCR102C(YCR102C_p)|FD-Score:3.44|P-value:2.92E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YFL051C(YFL051C_p)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YFR006W(YFR006W_p)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YHR080C(YHR080C)|FD-Score:-3.36|P-value:3.90E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YHR140W(YHR140W_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YIR018C-A(YIR018C-A_p)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YKL107W(YKL107W_p)|FD-Score:4.31|P-value:8.06E-6||SGD DESC:Putative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein Gene:YLR202C(YLR202C_d)|FD-Score:5.44|P-value:2.68E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YLR279W(YLR279W_d)|FD-Score:-4.61|P-value:1.99E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML018C(YML018C_p)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YNL144C(YNL144C_p)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study Gene:YOL118C(YOL118C_d)|FD-Score:-4.66|P-value:1.57E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR052C(YOR052C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Nuclear protein of unknown function; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner and by weak acid; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YSC83(YHR017W)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL247W7.378.60E-142.49YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YOR319W4.875.45E-70.54HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YGR156W4.347.26E-60.15PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YOL021C4.191.41E-50.05DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YOL038W4.141.76E-50.18PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YPL028W3.953.83E-50.05ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YGR090W3.904.79E-50.01UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YPL252C3.895.09E-50.08YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
YMR277W3.817.02E-50.01FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YPL204W3.807.30E-50.14HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YKR063C3.661.25E-40.03LAS1Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability
YLR132C3.631.41E-40.14USB1Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants
YKL112W3.492.45E-40.01ABF1DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
YPL251W_d3.482.55E-40.01YPL251W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
YJL085W3.462.66E-40.03EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR312W7.096.86E-13ELP6Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
YDR363W6.494.42E-11ESC2Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member
YAL027W6.038.46E-10SAW15'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
YLR202C_d5.442.68E-8YLR202C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
YER145C5.373.91E-8FTR1High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress
YOR134W5.267.40E-8BAG7Rho GTPase activating protein (RhoGAP), stimulates the intrinsic GTPase activity of Rho1p, which plays a role in actin cytoskeleton organization and control of cell wall synthesis; structurally and functionally related to Sac7p
YHR017W4.651.63E-6YSC83Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83
YPR049C4.454.27E-6ATG11Adapter protein for pexophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the (PAS)
YMR299C4.434.77E-6DYN3Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
YPR157W_p4.347.14E-6TDA6_pPutative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele
YAR035W4.347.24E-6YAT1Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated
YKL107W_p4.318.06E-6YKL107W_pPutative short-chain dehydrogenase/reductase; proposed to be a palmitoylated membrane protein
YIR018C-A_p4.141.71E-5YIR018C-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YGL060W4.003.23E-5YBP2Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication
YJL044C3.953.86E-5GYP6GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport

GO enrichment analysis for SGTC_1611
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0905.56E-12SGTC_1083didemethylchlorpromazine sulfoxide 707.4 nMTimTec (Natural product library)3987880.0615385redox potentiating
0.0731.59E-8SGTC_30099080819 71.4 μMChembridge (Drug-like library)170272160.0909091
0.0588.20E-6SGTC_2615-fluorouridine 15.4 μMMiscellaneous18210.0793651exosome
0.0534.82E-5SGTC_259usnic acid 108.9 μMMiscellaneous56460.119403
0.0526.20E-5SGTC_6173770-0098 35.0 μMChemDiv (Drug-like library)59626390.132353exosome
0.0526.20E-5SGTC_20345197015 53.1 μMChembridge (Fragment library)7806760.116667SWF1 & branched chain AA biosynthesis
0.0527.02E-5SGTC_29237992654 58.1 μMChembridge (Drug-like library)29851260.112903Golgi
0.0527.03E-5SGTC_485niflumic acid 177.0 μMMiscellaneous44880.09375
0.0501.06E-4SGTC_31429086498 49.5 μMChembridge (Drug-like library)252363670.0833333iron homeostasis
0.0501.16E-4SGTC_23027771753 200.0 μMChembridge (Fragment library)22030470.113208
0.0491.67E-4SGTC_12680828-0275 2.5 μMChemDiv (Drug-like library)7453370.140351
0.0491.84E-4SGTC_1685st024049 53.1 μMTimTec (Natural product derivative library)19411390.123288TSC3-RPN4
0.0482.15E-4SGTC_9691313-0238 134.0 μMChemDiv (Drug-like library)52128740.0724638mitochondrial processes
0.0473.20E-4SGTC_28287998861 71.4 μMChembridge (Drug-like library)29881880.125
0.0463.82E-4SGTC_28237987624 58.4 μMChembridge (Drug-like library)22394400.0833333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_3247913514949.47 μM0.3023268934436Chembridge (Drug-like library)220.226123.42503DNA damage response
SGTC_2279794842332.93 μM0.2857141247112Chembridge (Fragment library)260.311583.18314
SGTC_22406690706185.95 μM0.280702805876Chembridge (Fragment library)266.294562.55213
SGTC_2981391-072910 μM0.2692313237558ChemDiv (Drug-like library)263.247563.3104calcium & mitochondrial duress
SGTC_2139557869256.47 μM0.26650991Chembridge (Fragment library)207.248962.1904superoxide
SGTC_3198911201949.47 μM0.25862145595555Chembridge (Drug-like library)315.754263.43425mitochondrial processes
SGTC_2803448-36073.04 μM0.254237626179ChemDiv (Drug-like library)293.273543.2940560S ribosome export
SGTC_10953-[3,5-dibromo-4-hydroxybenzoyl]-2-ethylbenzofuran15.5 μM0.2456142333NIH Clinical Collection424.083385.60413
SGTC_1678st01926580.2 μM0.245367020TimTec (Natural product derivative library)249.264043.6080360S ribosome export
SGTC_3088911757849.47 μM0.24678341Chembridge (Drug-like library)251.279924.11303amide catabolism