st002383

N-[2-(diethylamino)ethyl]-3-methyl-5-phenylmethoxy-1H-indole-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1614
Screen concentration 48.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 1947235
SMILES CCN(CC)CCNC(=O)C1=C(C2=C(N1)C=CC(=C2)OCC3=CC=CC=C3)C
Standardized SMILES CCN(CC)CCNC(=O)c1[nH]c2ccc(OCc3ccccc3)cc2c1C
Molecular weight 379.4953
ALogP 4.51
H-bond donor count 2
H-bond acceptor count 3
Response signature cell wall signaling

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.56
% growth inhibition (Hom. pool) 9.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1947235
Download HIP data (tab-delimited text)  (excel)
Gene:CAB3(YKL088W)|FD-Score:-3.13|P-value:8.70E-4|Clearance:0||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:DOP1(YDR141C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.42||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:PRE2(YPR103W)|FD-Score:-4.43|P-value:4.65E-6|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RAD3(YER171W)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RAT1(YOR048C)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.42||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RIM2(YBR192W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.42||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPP1(YHR062C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.42||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:YGR114C(YGR114C_d)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.42||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:CAB3(YKL088W)|FD-Score:-3.13|P-value:8.70E-4|Clearance:0||SGD DESC:Subunit of a phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaBC Gene:DOP1(YDR141C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.42||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:PRE2(YPR103W)|FD-Score:-4.43|P-value:4.65E-6|Clearance:0||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RAD3(YER171W)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RAT1(YOR048C)|FD-Score:3.45|P-value:2.82E-4|Clearance:0.42||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RIM2(YBR192W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.42||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPP1(YHR062C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.42||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:YGR114C(YGR114C_d)|FD-Score:3.63|P-value:1.41E-4|Clearance:0.42||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1947235
Download HOP data (tab-delimited text)  (excel)
Gene:ABZ2(YMR289W)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ARO3(YDR035W)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BST1(YFL025C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD14(YAR014C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CHA1(YCL064C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CIN8(YEL061C)|FD-Score:4.54|P-value:2.81E-6||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CNM67(YNL225C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CYS4(YGR155W)|FD-Score:6.03|P-value:8.45E-10||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL2(YIR029W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:6.02|P-value:8.46E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIE2(YGR227W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DOA4(YDR069C)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DSS1(YMR287C)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:DUF1(YOL087C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ERG2(YMR202W)|FD-Score:10.4|P-value:9.62E-26||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FYV8(YGR196C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:4.77|P-value:9.17E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GET2(YER083C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GRX4(YER174C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HER2(YMR293C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:LST7(YGR057C)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MHP1(YJL042W)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MRPL37(YBR268W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB2(YGR014W)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:PDC6(YGR087C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PET54(YGR222W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PMT1(YDL095W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PPS1(YBR276C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:QCR7(YDR529C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RGD1(YBR260C)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RMT2(YDR465C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RTC6(YPL183W-A)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTT103(YDR289C)|FD-Score:4.34|P-value:7.18E-6||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAC1(YKL212W)|FD-Score:5|P-value:2.89E-7||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCJ1(YMR214W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SLG1(YOR008C)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:19.2|P-value:1.29E-82||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:STF1(YDL130W-A)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SWS2(YNL081C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TAT1(YBR069C)|FD-Score:7.02|P-value:1.12E-12||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:6.23|P-value:2.28E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR239C(YDR239C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YER077C(YER077C_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGL034C(YGL034C_d)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL118W(YKL118W_d)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR040C(YKR040C_d)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YML007C-A(YML007C-A_p)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YPL205C(YPL205C_d)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPL260W(YPL260W_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR197C(YPR197C_d)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ABZ2(YMR289W)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ARO3(YDR035W)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BST1(YFL025C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BUD14(YAR014C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:3.4|P-value:3.32E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CHA1(YCL064C)|FD-Score:3.33|P-value:4.34E-4||SGD DESC:Catabolic L-serine (L-threonine) deaminase, catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine Gene:CIN8(YEL061C)|FD-Score:4.54|P-value:2.81E-6||SGD DESC:Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation Gene:CNM67(YNL225C)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:CYS4(YGR155W)|FD-Score:6.03|P-value:8.45E-10||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DAL2(YIR029W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Allantoicase, converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAL81(YIR023W)|FD-Score:6.02|P-value:8.46E-10||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIE2(YGR227W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1 Gene:DOA4(YDR069C)|FD-Score:3.11|P-value:9.42E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DSS1(YMR287C)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs Gene:DUF1(YOL087C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:ERG2(YMR202W)|FD-Score:10.4|P-value:9.62E-26||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.96|P-value:3.77E-5||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FYV8(YGR196C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GCN20(YFR009W)|FD-Score:4.77|P-value:9.17E-7||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GET2(YER083C)|FD-Score:3.14|P-value:8.35E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GRX4(YER174C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HER2(YMR293C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:LST7(YGR057C)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MHP1(YJL042W)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MRPL37(YBR268W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB2(YGR014W)|FD-Score:-3.09|P-value:9.94E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:PDC6(YGR087C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PET54(YGR222W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PMT1(YDL095W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PPS1(YBR276C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:QCR7(YDR529C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RGD1(YBR260C)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization Gene:RMT2(YDR465C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RTC6(YPL183W-A)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTT103(YDR289C)|FD-Score:4.34|P-value:7.18E-6||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAC1(YKL212W)|FD-Score:5|P-value:2.89E-7||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SCJ1(YMR214W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins Gene:SLG1(YOR008C)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:19.2|P-value:1.29E-82||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:STF1(YDL130W-A)|FD-Score:3.47|P-value:2.65E-4||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:SWS2(YNL081C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:TAT1(YBR069C)|FD-Score:7.02|P-value:1.12E-12||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:YDR008C(YDR008C_d)|FD-Score:6.23|P-value:2.28E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR239C(YDR239C)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments Gene:YER077C(YER077C_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport Gene:YGL034C(YGL034C_d)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL118W(YKL118W_d)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR040C(YKR040C_d)|FD-Score:3.75|P-value:8.80E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W Gene:YML007C-A(YML007C-A_p)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YPL205C(YPL205C_d)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPL260W(YPL260W_p)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YPR197C(YPR197C_d)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C3.721.01E-40.42RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR114C_d3.631.41E-40.42YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YBR192W3.621.50E-40.42RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YDR141C3.462.72E-40.42DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
YOR048C3.452.82E-40.42RAT1Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination
YNL124W3.030.001240.00NAF1RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
YKL165C3.020.001250.03MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YNL262W2.990.001380.10POL2Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
YDL097C2.890.001920.18RPN6Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
YIL026C2.710.003410.08IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YPR055W2.620.004380.04SEC8Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
YPR165W2.580.004980.07RHO1GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
YPL204W2.510.006050.02HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YBR109C2.490.006320.00CMD1Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin
YDL143W2.490.006360.03CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR030C19.201.29E-82SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YMR202W10.409.62E-26ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YBR069C7.021.12E-12TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YDR008C_d6.232.28E-10YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YGR155W6.038.45E-10CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YIR023W6.028.46E-10DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YKL212W5.002.89E-7SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YFR009W4.779.17E-7GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YOR008C4.592.25E-6SLG1Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
YEL061C4.542.81E-6CIN8Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
YBR260C4.493.60E-6RGD1GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
YDR289C4.347.18E-6RTT103Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
YMR214W4.141.75E-5SCJ1One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
YDL095W4.102.09E-5PMT1Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
YGL034C_d4.013.03E-5YGL034C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_1614
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3644.56E-184SGTC_500h-89 112.0 μMICCB bioactive library4492410.0967742cell wall signaling
0.3211.49E-141SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.0961538cell wall signaling
0.3006.80E-123SGTC_22107115885 190.4 μMChembridge (Fragment library)29270390.0547945cell wall signaling
0.2809.79E-107SGTC_6631486-0733 13.9 μMChemDiv (Drug-like library)7224450.122222cell wall signaling
0.2771.55E-104SGTC_21495554786 194.8 μMChembridge (Fragment library)7450630.075cell wall signaling
0.2719.08E-100SGTC_3690957-0393 4.5 μMChemDiv (Drug-like library)69979920.0679612cell wall signaling
0.2651.33E-95SGTC_29249010168 62.8 μMChembridge (Drug-like library)29939290.246575cell wall signaling
0.2581.68E-90SGTC_2776terbinafine 19.9 μMNIH Clinical Collection52824810.109756cell wall signaling
0.2402.79E-78SGTC_6420335-0876 158.0 μMChemDiv (Drug-like library)68123500.0674157ubiquinone biosynthesis & proteasome
0.2374.28E-76SGTC_1907st060210 83.7 μMTimTec (Natural product derivative library)6887930.148649
0.2356.89E-75SGTC_23007976697 71.6 μMChembridge (Fragment library)22361090.0779221ERG2
0.2333.02E-73SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.105263ERG2
0.2312.23E-72SGTC_9052-aminofluorene 215.0 μMChemDiv (Drug-like library)15390.0869565cell wall signaling
0.2301.62E-71SGTC_790052-0007 94.1 μMChemDiv (Drug-like library)28014720.135135
0.2183.02E-64SGTC_1090132-0036 33.7 μMChemDiv (Drug-like library)672740.173333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1613st00238152.1 μM0.553846551167TimTec (Natural product derivative library)363.45283.83913
SGTC_150266-008627.1 μM0.3289471712902ChemDiv (Drug-like library)378.50725.23603endomembrane recycling
SGTC_3260913710949.47 μM0.32812517478346Chembridge (Drug-like library)255.311683.1561260S ribosome export
SGTC_2981901395238.96 μM0.3142862996344Chembridge (Drug-like library)348.234324.39112
SGTC_2888905854712.99 μM0.30434816476514Chembridge (Drug-like library)279.333084.11812
SGTC_2757clomiphene4.22 μM0.2941181548953Miscellaneous405.959626.48302fatty acid desaturase (OLE1)
SGTC_3171910319249.47 μM0.28571413018434Chembridge (Drug-like library)269.338263.50512
SGTC_3207911416349.47 μM0.285714849233Chembridge (Drug-like library)269.338263.43812
SGTC_3184910875849.47 μM0.283582902885Chembridge (Drug-like library)241.28512.94712
SGTC_3310913022944.14 μM0.281697761258Chembridge (Drug-like library)289.756743.8212Golgi