st003704

(5-methyl-2-propan-2-ylcyclohexyl) 2-[2-(4-bromophenyl)-3-methylbenzimidazol-3-ium-1-yl]acetate chloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1620
Screen concentration 16.5 μM
Source TimTec (Natural product derivative library)
PubChem CID 2834414
SMILES CC1CCC(C(C1)OC(=O)CN2C3=CC=CC=C3[N+](=C2C4=CC=C(C=C4)Br)C)C(C)C.[Cl-]
Standardized SMILES CC(C)C1CCC(C)CC1OC(=O)Cn2c(c3ccc(Br)cc3)[n+](C)c4ccccc24
Molecular weight 519.9015
ALogP 4.83
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.15
% growth inhibition (Hom. pool) 4.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2834414
Download HIP data (tab-delimited text)  (excel)
Gene:CDC24(YAL041W)|FD-Score:-4|P-value:3.11E-5|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DPS1(YLL018C)|FD-Score:3.38|P-value:3.60E-4|Clearance:0.01||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:HRR25(YPL204W)|FD-Score:3.4|P-value:3.36E-4|Clearance:0.02||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:KAR2(YJL034W)|FD-Score:5.97|P-value:1.15E-9|Clearance:0.92||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:MTR10(YOR160W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.03||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:PRE2(YPR103W)|FD-Score:3.13|P-value:8.84E-4|Clearance:0.18||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RIM2(YBR192W)|FD-Score:5.06|P-value:2.10E-7|Clearance:0.66||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RNT1(YMR239C)|FD-Score:3.91|P-value:4.65E-5|Clearance:0.51||SGD DESC:Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes Gene:RPP1(YHR062C)|FD-Score:-3.11|P-value:9.46E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRN6(YBL014C)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.01||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RTT105(YER104W)|FD-Score:3.29|P-value:4.97E-4|Clearance:0.1||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SRP68(YPL243W)|FD-Score:4.4|P-value:5.45E-6|Clearance:0.49||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:TIF35(YDR429C)|FD-Score:-3.26|P-value:5.65E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRS23(YDR246W)|FD-Score:3.2|P-value:6.94E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:YCG1(YDR325W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.04||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:ZIM17(YNL310C)|FD-Score:3.19|P-value:7.02E-4|Clearance:0.07||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix Gene:CDC24(YAL041W)|FD-Score:-4|P-value:3.11E-5|Clearance:0||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DPS1(YLL018C)|FD-Score:3.38|P-value:3.60E-4|Clearance:0.01||SGD DESC:Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation Gene:HRR25(YPL204W)|FD-Score:3.4|P-value:3.36E-4|Clearance:0.02||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:KAR2(YJL034W)|FD-Score:5.97|P-value:1.15E-9|Clearance:0.92||SGD DESC:ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p Gene:MTR10(YOR160W)|FD-Score:3.32|P-value:4.45E-4|Clearance:0.03||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:PRE2(YPR103W)|FD-Score:3.13|P-value:8.84E-4|Clearance:0.18||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RIM2(YBR192W)|FD-Score:5.06|P-value:2.10E-7|Clearance:0.66||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RNT1(YMR239C)|FD-Score:3.91|P-value:4.65E-5|Clearance:0.51||SGD DESC:Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes Gene:RPP1(YHR062C)|FD-Score:-3.11|P-value:9.46E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRN6(YBL014C)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.01||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RTT105(YER104W)|FD-Score:3.29|P-value:4.97E-4|Clearance:0.1||SGD DESC:Protein with a role in regulation of Ty1 transposition Gene:SRP68(YPL243W)|FD-Score:4.4|P-value:5.45E-6|Clearance:0.49||SGD DESC:Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:TIF35(YDR429C)|FD-Score:-3.26|P-value:5.65E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TRS23(YDR246W)|FD-Score:3.2|P-value:6.94E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:YCG1(YDR325W)|FD-Score:3.37|P-value:3.75E-4|Clearance:0.04||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:ZIM17(YNL310C)|FD-Score:3.19|P-value:7.02E-4|Clearance:0.07||SGD DESC:Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2834414
Download HOP data (tab-delimited text)  (excel)
Gene:AAT1(YKL106W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis Gene:ADA2(YDR448W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AIM18(YHR198C)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALG5(YPL227C)|FD-Score:4.86|P-value:5.97E-7||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:AMD2(YDR242W)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Putative amidase Gene:ARO80(YDR421W)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:CBR1(YIL043C)|FD-Score:-4.61|P-value:2.01E-6||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCS1(YMR038C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CMC4(YMR194C-B)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CMS1(YLR003C)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:COX20(YDR231C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase Gene:CWH43(YCR017C)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:CYB5(YNL111C)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DNM1(YLL001W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Gene:ERG2(YMR202W)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ESC1(YMR219W)|FD-Score:-6.43|P-value:6.43E-11||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:EXG2(YDR261C)|FD-Score:3.79|P-value:7.54E-5||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FAT1(YBR041W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FEN2(YCR028C)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:GET3(YDL100C)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GIS2(YNL255C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GYL1(YMR192W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HHF1(YBR009C)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HIM1(YDR317W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:ICL1(YER065C)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:IMP2'(YIL154C)|FD-Score:7.32|P-value:1.20E-13||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP51(YIL002C)|FD-Score:4.96|P-value:3.45E-7||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:MSN2(YMR037C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:NPL3(YDR432W)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PDR1(YGL013C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX31(YGR004W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PPS1(YBR276C)|FD-Score:4|P-value:3.19E-5||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:PYC2(YBR218C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAD51(YER095W)|FD-Score:4.39|P-value:5.62E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RCE1(YMR274C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RCK2(YLR248W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RGL1(YPL066W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIC1(YLR039C)|FD-Score:-3.74|P-value:9.16E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL17B(YJL177W)|FD-Score:4.6|P-value:2.11E-6||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:SER1(YOR184W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SHM1(YBR263W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine Gene:TPN1(YGL186C)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRS85(YDR108W)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TSL1(YML100W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:UPS3(YDR185C)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VPS61(YDR136C_d)|FD-Score:-3.81|P-value:6.88E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR300C(YBR300C_d)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCR025C(YCR025C_d)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YCR061W(YCR061W_p)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR220C(YDR220C_d)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YER121W(YER121W_p)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YFR006W(YFR006W_p)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGR051C(YGR051C_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YHR140W(YHR140W_p)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YKR032W(YKR032W_d)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR047W(YKR047W_d)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR122C(YLR122C_d)|FD-Score:-3.78|P-value:7.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR194C(YLR194C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YMR105W-A(YMR105W-A_p)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Putative protein of unknown function Gene:YNL285W(YNL285W_d)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR114W(YOR114W_p)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:ZUO1(YGR285C)|FD-Score:5.49|P-value:2.00E-8||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:AAT1(YKL106W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis Gene:ADA2(YDR448W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AIM18(YHR198C)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALG5(YPL227C)|FD-Score:4.86|P-value:5.97E-7||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:AMD2(YDR242W)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Putative amidase Gene:ARO80(YDR421W)|FD-Score:3.95|P-value:3.90E-5||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:CBR1(YIL043C)|FD-Score:-4.61|P-value:2.01E-6||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CCS1(YMR038C)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CMC4(YMR194C-B)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CMS1(YLR003C)|FD-Score:-4.3|P-value:8.44E-6||SGD DESC:Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:COX20(YDR231C)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase Gene:CWH43(YCR017C)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:CYB5(YNL111C)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DNM1(YLL001W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Gene:ERG2(YMR202W)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ESC1(YMR219W)|FD-Score:-6.43|P-value:6.43E-11||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:EXG2(YDR261C)|FD-Score:3.79|P-value:7.54E-5||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FAT1(YBR041W)|FD-Score:-3.49|P-value:2.41E-4||SGD DESC:Very long chain fatty acyl-CoA synthetase and long chain fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids Gene:FEN2(YCR028C)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:GET3(YDL100C)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GIS2(YNL255C)|FD-Score:3.2|P-value:6.78E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GYL1(YMR192W)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HHF1(YBR009C)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HIM1(YDR317W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:ICL1(YER065C)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:IMP2'(YIL154C)|FD-Score:7.32|P-value:1.20E-13||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP51(YIL002C)|FD-Score:4.96|P-value:3.45E-7||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:MSN2(YMR037C)|FD-Score:3.43|P-value:3.02E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:NPL3(YDR432W)|FD-Score:3.76|P-value:8.50E-5||SGD DESC:RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PAR32(YDL173W)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PDR1(YGL013C)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEX31(YGR004W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p Gene:PPS1(YBR276C)|FD-Score:4|P-value:3.19E-5||SGD DESC:Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle Gene:PYC2(YBR218C)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAD51(YER095W)|FD-Score:4.39|P-value:5.62E-6||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RCE1(YMR274C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RCK2(YLR248W)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RGL1(YPL066W)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RIC1(YLR039C)|FD-Score:-3.74|P-value:9.16E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL17B(YJL177W)|FD-Score:4.6|P-value:2.11E-6||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:SER1(YOR184W)|FD-Score:-3.65|P-value:1.29E-4||SGD DESC:3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress Gene:SHM1(YBR263W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine Gene:TPN1(YGL186C)|FD-Score:-3.14|P-value:8.45E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRS85(YDR108W)|FD-Score:3.73|P-value:9.61E-5||SGD DESC:Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role Gene:TSL1(YML100W)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:UPS3(YDR185C)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:VPS61(YDR136C_d)|FD-Score:-3.81|P-value:6.88E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YBR300C(YBR300C_d)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene Gene:YCR025C(YCR025C_d)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene Gene:YCR061W(YCR061W_p)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR220C(YDR220C_d)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YER121W(YER121W_p)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YFR006W(YFR006W_p)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGR051C(YGR051C_d)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene Gene:YHR140W(YHR140W_p)|FD-Score:3.22|P-value:6.47E-4||SGD DESC:Putative integral membrane protein of unknown function Gene:YKR032W(YKR032W_d)|FD-Score:3.24|P-value:5.89E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKR047W(YKR047W_d)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR122C(YLR122C_d)|FD-Score:-3.78|P-value:7.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR194C(YLR194C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YMR105W-A(YMR105W-A_p)|FD-Score:4.06|P-value:2.47E-5||SGD DESC:Putative protein of unknown function Gene:YNL285W(YNL285W_d)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR114W(YOR114W_p)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Putative protein of unknown function; null mutant is viable Gene:ZUO1(YGR285C)|FD-Score:5.49|P-value:2.00E-8||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL034W5.971.15E-90.92KAR2ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
YBR192W5.062.10E-70.66RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YPL243W4.405.45E-60.49SRP68Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YMR239C3.914.65E-50.51RNT1Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes
YPL204W3.403.36E-40.02HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YLL018C3.383.60E-40.01DPS1Aspartyl-tRNA synthetase, primarily cytoplasmic; homodimeric enzyme that catalyzes the specific aspartylation of tRNA(Asp); class II aminoacyl tRNA synthetase; binding to its own mRNA may confer autoregulation
YDR325W3.373.75E-40.04YCG1Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding of the condensin complex; required for clustering of tRNA genes at the nucleolus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YBL014C3.334.35E-40.01RRN6Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YOR160W3.324.45E-40.03MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YER104W3.294.97E-40.10RTT105Protein with a role in regulation of Ty1 transposition
YDR246W3.206.94E-40.00TRS23One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4
YNL310C3.197.02E-40.07ZIM17Heat shock protein with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix
YPR103W3.138.84E-40.18PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YBR029C2.950.001590.08CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
YOR250C2.870.002050.10CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL154C7.321.20E-13IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YGR285C5.492.00E-8ZUO1Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
YBR009C5.121.53E-7HHF1Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
YMR202W5.091.76E-7ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YIL002C4.963.45E-7INP51Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
YPL227C4.865.97E-7ALG5UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum
YER065C4.651.66E-6ICL1Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose
YJL177W4.602.11E-6RPL17BRibosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication
YER095W4.395.62E-6RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YOR114W_p4.141.76E-5YOR114W_pPutative protein of unknown function; null mutant is viable
YCR025C_d4.082.25E-5YCR025C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YCR025C is not an essential gene
YMR105W-A_p4.062.47E-5YMR105W-A_pPutative protein of unknown function
YBR276C4.003.19E-5PPS1Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle
YDR220C_d3.963.73E-5YDR220C_dDubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein
YDR421W3.953.90E-5ARO80Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids

GO enrichment analysis for SGTC_1620
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2039.94E-56SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.0142857calcium & mitochondrial duress
0.1844.26E-46SGTC_400oxethazaine 37.5 μMMiscellaneous46210.119048calcium & mitochondrial duress
0.1633.43E-36SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.0947368calcium & mitochondrial duress
0.1529.48E-32SGTC_9943910-0338 138.0 μMChemDiv (Drug-like library)34794810.148936calcium & mitochondrial duress
0.1507.12E-31SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.151163ERG2
0.1482.68E-30SGTC_7014424-0219 185.0 μMChemDiv (Drug-like library)29117790.0909091calcium & mitochondrial duress
0.1441.66E-28SGTC_1613st002381 52.1 μMTimTec (Natural product derivative library)5511670.126316
0.1404.41E-27SGTC_1621st003705 32.4 μMTimTec (Natural product derivative library)242070320.854839calcium & mitochondrial duress
0.1218.29E-21SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0843373ERG2
0.1211.05E-20SGTC_330986-0033 35.8 μMChemDiv (Drug-like library)28480820.164706calcium & mitochondrial duress
0.1193.58E-20SGTC_6090986-0026 86.3 μMChemDiv (Drug-like library)28486100.177215calcium & mitochondrial duress
0.1172.13E-19SGTC_23007976697 71.6 μMChembridge (Fragment library)22361090.1125ERG2
0.1156.25E-19SGTC_1666st014829 29.0 μMTimTec (Natural product derivative library)38582300.0679612calcium & mitochondrial duress
0.1095.21E-17SGTC_1622st003707 19.4 μMTimTec (Natural product derivative library)101408990.630137calcium & mitochondrial duress
0.1081.00E-16SGTC_5820987-0078 156.0 μMChemDiv (Drug-like library)16257650.122222calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1621st00370532.4 μM0.85483924207032TimTec (Natural product derivative library)455.0324.5703calcium & mitochondrial duress
SGTC_1622st00370719.4 μM0.63013710140899TimTec (Natural product derivative library)485.057984.40504calcium & mitochondrial duress
SGTC_1624st0037106.25 μM0.55844224207033TimTec (Natural product derivative library)549.927464.66704calcium & mitochondrial duress
SGTC_1625st00371130.1 μM0.46913624207034TimTec (Natural product derivative library)501.057383.90205calcium & mitochondrial duress
SGTC_1627st00371325.6 μM0.46913616195070TimTec (Natural product derivative library)501.057383.90205calcium & mitochondrial duress
SGTC_1626st0037121.76 μM0.46341524207035TimTec (Natural product derivative library)505.476464.58304
SGTC_1623st00370920.3 μM0.45238116192618TimTec (Natural product derivative library)485.057984.40504calcium & mitochondrial duress
SGTC_15034585-001261.3 μM0.36252922052ChemDiv (Drug-like library)338.464943.81214
SGTC_1812st0520574.33 μM0.3176473645418TimTec (Natural product derivative library)422.664224.01203calcium & mitochondrial duress
SGTC_2641levomentholum100 μM0.23437516666Microsource (Natural product library)156.26522.77911