st003705

(5-methyl-2-propan-2-ylcyclohexyl) 2-[3-methyl-2-(4-methylphenyl)benzimidazol-3-ium-1-yl]acetate chloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1621
Screen concentration 32.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 24207032
SMILES CC1CCC(C(C1)OC(=O)CN2C3=CC=CC=C3[N+](=C2C4=CC=C(C=C4)C)C)C(C)C.[Cl-]
Standardized SMILES CC(C)C1CCC(C)CC1OC(=O)Cn2c(c3ccc(C)cc3)[n+](C)c4ccccc24
Molecular weight 455.032
ALogP 4.57
H-bond donor count 0
H-bond acceptor count 3
Response signature calcium & mitochondrial duress

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.87
% growth inhibition (Hom. pool) 7.69


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24207032
Download HIP data (tab-delimited text)  (excel)
Gene:ADE13(YLR359W)|FD-Score:3.85|P-value:5.81E-5|Clearance:0.1||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:GLC7(YER133W)|FD-Score:8.79|P-value:7.49E-19|Clearance:4.12||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IRR1(YIL026C)|FD-Score:4.08|P-value:2.29E-5|Clearance:0.16||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MCD4(YKL165C)|FD-Score:3.35|P-value:4.02E-4|Clearance:0.02||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM7(YBR202W)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.05||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MIA40(YKL195W)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.02||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NBP35(YGL091C)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.14||SGD DESC:Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases Gene:PRE6(YOL038W)|FD-Score:3.89|P-value:5.08E-5|Clearance:0.03||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRE8(YML092C)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RIM2(YBR192W)|FD-Score:4.67|P-value:1.52E-6|Clearance:0.59||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPC53(YDL150W)|FD-Score:3.92|P-value:4.51E-5|Clearance:0.03||SGD DESC:RNA polymerase III subunit C53 Gene:RPS2(YGL123W)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPT4(YOR259C)|FD-Score:3.75|P-value:8.80E-5|Clearance:0.18||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SEC4(YFL005W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.02||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SEC65(YML105C)|FD-Score:-4.7|P-value:1.29E-6|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SMD1(YGR074W)|FD-Score:3.33|P-value:4.32E-4|Clearance:0.14||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SPC24(YMR117C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:TFA2(YKR062W)|FD-Score:3.17|P-value:7.72E-4|Clearance:0.21||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:YLR458W(YLR458W_d)|FD-Score:-3.73|P-value:9.42E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis Gene:ADE13(YLR359W)|FD-Score:3.85|P-value:5.81E-5|Clearance:0.1||SGD DESC:Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency Gene:GLC7(YER133W)|FD-Score:8.79|P-value:7.49E-19|Clearance:4.12||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:IRR1(YIL026C)|FD-Score:4.08|P-value:2.29E-5|Clearance:0.16||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MCD4(YKL165C)|FD-Score:3.35|P-value:4.02E-4|Clearance:0.02||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM7(YBR202W)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.05||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:MIA40(YKL195W)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.02||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NBP35(YGL091C)|FD-Score:3.55|P-value:1.96E-4|Clearance:0.14||SGD DESC:Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases Gene:PRE6(YOL038W)|FD-Score:3.89|P-value:5.08E-5|Clearance:0.03||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRE8(YML092C)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RIM2(YBR192W)|FD-Score:4.67|P-value:1.52E-6|Clearance:0.59||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPC53(YDL150W)|FD-Score:3.92|P-value:4.51E-5|Clearance:0.03||SGD DESC:RNA polymerase III subunit C53 Gene:RPS2(YGL123W)|FD-Score:-3.63|P-value:1.39E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPT4(YOR259C)|FD-Score:3.75|P-value:8.80E-5|Clearance:0.18||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:SEC4(YFL005W)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.02||SGD DESC:Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane Gene:SEC65(YML105C)|FD-Score:-4.7|P-value:1.29E-6|Clearance:0||SGD DESC:Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19 Gene:SMD1(YGR074W)|FD-Score:3.33|P-value:4.32E-4|Clearance:0.14||SGD DESC:Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress Gene:SPC24(YMR117C)|FD-Score:-3.55|P-value:1.96E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:TFA2(YKR062W)|FD-Score:3.17|P-value:7.72E-4|Clearance:0.21||SGD DESC:TFIIE small subunit, involved in RNA polymerase II transcription initiation Gene:YLR458W(YLR458W_d)|FD-Score:-3.73|P-value:9.42E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1 gene required for mitosis

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24207032
Download HOP data (tab-delimited text)  (excel)
Gene:APC9(YLR102C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:ATP25(YMR098C)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BUD14(YAR014C)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CBR1(YIL043C)|FD-Score:-3.79|P-value:7.59E-5||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CIN4(YMR138W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CPR6(YLR216C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress Gene:CYS3(YAL012W)|FD-Score:7.41|P-value:6.41E-14||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:5.96|P-value:1.27E-9||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DIN7(YDR263C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:ECM33(YBR078W)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERG2(YMR202W)|FD-Score:7.44|P-value:5.00E-14||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GCN5(YGR252W)|FD-Score:4.28|P-value:9.31E-6||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GLO3(YER122C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GYP6(YJL044C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HRD3(YLR207W)|FD-Score:4.44|P-value:4.54E-6||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:IES3(YLR052W)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IMP2'(YIL154C)|FD-Score:13.9|P-value:1.67E-44||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC3(YDR332W)|FD-Score:4.28|P-value:9.41E-6||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:JEM1(YJL073W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KNS1(YLL019C)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:MEF2(YJL102W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MOG1(YJR074W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRP2(YPR166C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL16(YBL038W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL23(YOR150W)|FD-Score:4.39|P-value:5.54E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL37(YBR268W)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS28(YDR337W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSW1(YDR268W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MXR2(YCL033C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NAS2(YIL007C)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:NCE101(YJL205C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NEM1(YHR004C)|FD-Score:4.27|P-value:9.86E-6||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NST1(YNL091W)|FD-Score:4.49|P-value:3.63E-6||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:OCA4(YCR095C_p)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OSH3(YHR073W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEA2(YER149C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PET112(YBL080C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET123(YOR158W)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PET54(YGR222W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PFA4(YOL003C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases Gene:PHO87(YCR037C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PML39(YML107C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:PSY1(YKL076C_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:QCR7(YDR529C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RPL7A(YGL076C)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPS26B(YER131W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:RSM28(YDR494W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:RSM7(YJR113C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTC6(YPL183W-A)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTT103(YDR289C)|FD-Score:6.93|P-value:2.17E-12||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SGT2(YOR007C)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SUV3(YPL029W)|FD-Score:7.47|P-value:4.06E-14||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SYG1(YIL047C)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:SYM1(YLR251W)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TRK1(YJL129C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:UBA4(YHR111W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:VID22(YLR373C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YBL095W(YBL095W_p)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBP2(YGL060W)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YEL020C(YEL020C_p)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YGL010W(YGL010W_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YIP4(YGL198W)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:ZUO1(YGR285C)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:APC9(YLR102C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:ATP25(YMR098C)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BUD14(YAR014C)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CBR1(YIL043C)|FD-Score:-3.79|P-value:7.59E-5||SGD DESC:Microsomal cytochrome b reductase, not essential for viability; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia Gene:CIN4(YMR138W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CPR6(YLR216C)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress Gene:CYS3(YAL012W)|FD-Score:7.41|P-value:6.41E-14||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:CYS4(YGR155W)|FD-Score:5.96|P-value:1.27E-9||SGD DESC:Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria Gene:DIN7(YDR263C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:ECM33(YBR078W)|FD-Score:5.17|P-value:1.16E-7||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ERG2(YMR202W)|FD-Score:7.44|P-value:5.00E-14||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:GCN5(YGR252W)|FD-Score:4.28|P-value:9.31E-6||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GLO3(YER122C)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GYP6(YJL044C)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HRD3(YLR207W)|FD-Score:4.44|P-value:4.54E-6||SGD DESC:Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events Gene:IES3(YLR052W)|FD-Score:-3.52|P-value:2.12E-4||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:IMP2'(YIL154C)|FD-Score:13.9|P-value:1.67E-44||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC3(YDR332W)|FD-Score:4.28|P-value:9.41E-6||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:JEM1(YJL073W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:KNS1(YLL019C)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:MEF2(YJL102W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MOG1(YJR074W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRP2(YPR166C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL16(YBL038W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL23(YOR150W)|FD-Score:4.39|P-value:5.54E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL37(YBR268W)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS17(YMR188C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPS28(YDR337W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSW1(YDR268W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MXR2(YCL033C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Methionine-R-sulfoxide reductase, involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan Gene:NAS2(YIL007C)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Proteasome-interacting protein; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); similar to mammalian proteasomal modulator subunit; non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress Gene:NCE101(YJL205C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NEM1(YHR004C)|FD-Score:4.27|P-value:9.86E-6||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NST1(YNL091W)|FD-Score:4.49|P-value:3.63E-6||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:OCA4(YCR095C_p)|FD-Score:-3.31|P-value:4.63E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:OSH3(YHR073W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEA2(YER149C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PET112(YBL080C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog Gene:PET123(YOR158W)|FD-Score:5.18|P-value:1.09E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PET54(YGR222W)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PFA4(YOL003C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases Gene:PHO87(YCR037C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PML39(YML107C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:PSY1(YKL076C_d)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C Gene:QCR7(YDR529C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RPL7A(YGL076C)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPS26B(YER131W)|FD-Score:-3.67|P-value:1.21E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication Gene:RSM28(YDR494W)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:RSM7(YJR113C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein Gene:RTC6(YPL183W-A)|FD-Score:4.75|P-value:1.01E-6||SGD DESC:Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress Gene:RTT103(YDR289C)|FD-Score:6.93|P-value:2.17E-12||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SGT2(YOR007C)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress Gene:SUV3(YPL029W)|FD-Score:7.47|P-value:4.06E-14||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SYG1(YIL047C)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:SYM1(YLR251W)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TRK1(YJL129C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TRM1(YDR120C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments Gene:UBA4(YHR111W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:VID22(YLR373C)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YBL095W(YBL095W_p)|FD-Score:3.84|P-value:6.24E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBP2(YGL060W)|FD-Score:4.48|P-value:3.65E-6||SGD DESC:Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.26|P-value:5.65E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YEL020C(YEL020C_p)|FD-Score:3.12|P-value:9.16E-4||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YGL010W(YGL010W_p)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YIP4(YGL198W)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YKL068W-A(YKL068W-A_p)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:ZUO1(YGR285C)|FD-Score:3.78|P-value:7.78E-5||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YER133W8.797.49E-194.12GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YBR192W4.671.52E-60.59RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YIL026C4.082.29E-50.16IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YDL150W3.924.51E-50.03RPC53RNA polymerase III subunit C53
YOL038W3.895.08E-50.03PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YLR359W3.855.81E-50.10ADE13Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
YOR259C3.758.80E-50.18RPT4One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
YFL005W3.571.79E-40.02SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YGL091C3.551.96E-40.14NBP35Essential iron-sulfur cluster binding protein localized in the cytoplasm; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases
YBR202W3.413.29E-40.05MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YKL165C3.354.02E-40.02MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YGR074W3.334.32E-40.14SMD1Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
YKL195W3.197.16E-40.02MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YKR062W3.177.72E-40.21TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YOL094C2.960.001557.71E-4RFC4Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YIL154C13.901.67E-44IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YPL029W7.474.06E-14SUV3ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron
YMR202W7.445.00E-14ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YAL012W7.416.41E-14CYS3Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
YDR289C6.932.17E-12RTT103Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
YGR155W5.961.27E-9CYS4Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
YOR158W5.181.09E-7PET123Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
YBR078W5.171.16E-7ECM33GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p
YPL183W-A4.751.01E-6RTC6Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
YBR268W4.621.93E-6MRPL37Mitochondrial ribosomal protein of the large subunit
YJR113C4.612.02E-6RSM7Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S7 ribosomal protein
YMR098C4.552.74E-6ATP25Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene
YNL091W4.493.63E-6NST1Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1
YGL060W4.483.65E-6YBP2Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication
YLR207W4.444.54E-6HRD3Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events

GO enrichment analysis for SGTC_1621
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3312.39E-150SGTC_1622st003707 19.4 μMTimTec (Natural product derivative library)101408990.735294calcium & mitochondrial duress
0.3111.82E-132SGTC_1623st003709 20.3 μMTimTec (Natural product derivative library)161926180.493827calcium & mitochondrial duress
0.2965.95E-120SGTC_400oxethazaine 37.5 μMMiscellaneous46210.120482calcium & mitochondrial duress
0.2926.90E-116SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.0144928calcium & mitochondrial duress
0.2691.78E-98SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.475calcium & mitochondrial duress
0.2624.50E-93SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.101124ERG2
0.2601.04E-91SGTC_6090986-0026 86.3 μMChemDiv (Drug-like library)28486100.210526calcium & mitochondrial duress
0.2481.08E-83SGTC_1624st003710 6.2 μMTimTec (Natural product derivative library)242070330.469136calcium & mitochondrial duress
0.2328.22E-73SGTC_6010986-0029 62.9 μMChemDiv (Drug-like library)247479810.177215calcium & mitochondrial duress
0.2217.98E-66SGTC_1666st014829 29.0 μMTimTec (Natural product derivative library)38582300.0686275calcium & mitochondrial duress
0.2196.32E-65SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.0957447calcium & mitochondrial duress
0.2105.98E-60SGTC_10573448-5381 134.0 μMChemDiv (Drug-like library)54979110.0697674calcium & mitochondrial duress
0.2093.12E-59SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0987654ERG2
0.2034.77E-56SGTC_9943910-0338 138.0 μMChemDiv (Drug-like library)34794810.126316calcium & mitochondrial duress
0.2034.78E-56SGTC_330986-0033 35.8 μMChemDiv (Drug-like library)28480820.166667calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1620st00370416.5 μM0.8548392834414TimTec (Natural product derivative library)519.901484.83203
SGTC_1622st00370719.4 μM0.73529410140899TimTec (Natural product derivative library)485.057984.40504calcium & mitochondrial duress
SGTC_1623st00370920.3 μM0.49382716192618TimTec (Natural product derivative library)485.057984.40504calcium & mitochondrial duress
SGTC_1625st00371130.1 μM0.47524207034TimTec (Natural product derivative library)501.057383.90205calcium & mitochondrial duress
SGTC_1627st00371325.6 μM0.47516195070TimTec (Natural product derivative library)501.057383.90205calcium & mitochondrial duress
SGTC_1624st0037106.25 μM0.46913624207033TimTec (Natural product derivative library)549.927464.66704calcium & mitochondrial duress
SGTC_1626st0037121.76 μM0.46913624207035TimTec (Natural product derivative library)505.476464.58304
SGTC_15034585-001261.3 μM0.3846152922052ChemDiv (Drug-like library)338.464943.81214
SGTC_1812st0520574.33 μM0.3214293645418TimTec (Natural product derivative library)422.664224.01203calcium & mitochondrial duress
SGTC_2641levomentholum100 μM0.23809516666Microsource (Natural product library)156.26522.77911