st003710

(5-methyl-2-propan-2-ylcyclohexyl) 2-[2-[(4-bromophenoxy)methyl]-3-methylbenzimidazol-3-ium-1-yl]acetate chloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1624
Screen concentration 6.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 24207033
SMILES CC1CCC(C(C1)OC(=O)CN2C3=CC=CC=C3[N+](=C2COC4=CC=C(C=C4)Br)C)C(C)C.[Cl-]
Standardized SMILES CC(C)C1CCC(C)CC1OC(=O)Cn2c(COc3ccc(Br)cc3)[n+](C)c4ccccc24
Molecular weight 549.9275
ALogP 4.67
H-bond donor count 0
H-bond acceptor count 4
Response signature calcium & mitochondrial duress

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.88
% growth inhibition (Hom. pool) 4.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24207033
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.57||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:GLC7(YER133W)|FD-Score:4.54|P-value:2.82E-6|Clearance:0.95||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:RPA43(YOR340C)|FD-Score:6.06|P-value:6.69E-10|Clearance:1.26||SGD DESC:RNA polymerase I subunit A43 Gene:SEC8(YPR055W)|FD-Score:-3.5|P-value:2.29E-4|Clearance:0||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SPC105(YGL093W)|FD-Score:4.8|P-value:7.82E-7|Clearance:0.26||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TFB3(YDR460W)|FD-Score:-4.51|P-value:3.27E-6|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:ARP2(YDL029W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.57||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:GLC7(YER133W)|FD-Score:4.54|P-value:2.82E-6|Clearance:0.95||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:RPA43(YOR340C)|FD-Score:6.06|P-value:6.69E-10|Clearance:1.26||SGD DESC:RNA polymerase I subunit A43 Gene:SEC8(YPR055W)|FD-Score:-3.5|P-value:2.29E-4|Clearance:0||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:SPC105(YGL093W)|FD-Score:4.8|P-value:7.82E-7|Clearance:0.26||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:TFB3(YDR460W)|FD-Score:-4.51|P-value:3.27E-6|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24207033
Download HOP data (tab-delimited text)  (excel)
Gene:ADA2(YDR448W)|FD-Score:4.81|P-value:7.72E-7||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AEP2(YMR282C)|FD-Score:5.81|P-value:3.11E-9||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AHC1(YOR023C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:APL5(YPL195W)|FD-Score:4.83|P-value:6.66E-7||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APN1(YKL114C)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:APQ13(YJL075C_d)|FD-Score:4.38|P-value:5.96E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ARG2(YJL071W)|FD-Score:4|P-value:3.22E-5||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARP1(YHR129C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ATG16(YMR159C)|FD-Score:5.54|P-value:1.54E-8||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATP15(YPL271W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CWH43(YCR017C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DNF1(YER166W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DUF1(YOL087C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EFG1(YGR271C-A)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ELM1(YKL048C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERG2(YMR202W)|FD-Score:14.6|P-value:1.88E-48||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG3(YLR056W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERG4(YGL012W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV14(YGL054C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ESC1(YMR219W)|FD-Score:10.7|P-value:3.01E-27||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:EXG2(YDR261C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FLD1(YLR404W)|FD-Score:7.58|P-value:1.71E-14||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FTR1(YER145C)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GCS1(YDL226C)|FD-Score:-3.92|P-value:4.41E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEP3(YOR205C)|FD-Score:5.8|P-value:3.40E-9||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GLO3(YER122C)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GYP6(YJL044C)|FD-Score:-4.85|P-value:6.32E-7||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HMI1(YOL095C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:IMP2'(YIL154C)|FD-Score:9.19|P-value:1.90E-20||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP51(YIL002C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRS4(YKR019C)|FD-Score:5.04|P-value:2.29E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KNS1(YLL019C)|FD-Score:-4.12|P-value:1.90E-5||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LPP1(YDR503C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:LRG1(YDL240W)|FD-Score:-4.52|P-value:3.08E-6||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MBP1(YDL056W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MEF1(YLR069C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MPC54(YOR177C)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRF1(YGL143C)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRP21(YBL090W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL16(YBL038W)|FD-Score:7.25|P-value:2.01E-13||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL27(YBR282W)|FD-Score:5.92|P-value:1.64E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL32(YCR003W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL4(YLR439W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPS12(YNR036C)|FD-Score:3.99|P-value:3.24E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSS116(YDR194C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MTQ2(YDR140W)|FD-Score:5.22|P-value:8.85E-8||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:NOT3(YIL038C)|FD-Score:6.2|P-value:2.89E-10||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OSH2(YDL019C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PIM1(YBL022C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PKP2(YGL059W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PML39(YML107C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:POP2(YNR052C)|FD-Score:7.62|P-value:1.27E-14||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PTC1(YDL006W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:QCR7(YDR529C)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RML2(YEL050C)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RSC1(YGR056W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RSM18(YER050C)|FD-Score:4.41|P-value:5.26E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM27(YGR215W)|FD-Score:4.87|P-value:5.48E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAC1(YKL212W)|FD-Score:6|P-value:1.01E-9||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SEC22(YLR268W)|FD-Score:5.81|P-value:3.13E-9||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SFB3(YHR098C)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SKM1(YOL113W)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLA1(YBL007C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLS1(YLR139C)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SSN2(YDR443C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSN3(YPL042C)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SUV3(YPL029W)|FD-Score:9.2|P-value:1.81E-20||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TAN1(YGL232W)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TGL2(YDR058C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TMA46(YOR091W)|FD-Score:7.73|P-value:5.24E-15||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRK1(YJL129C)|FD-Score:5.35|P-value:4.28E-8||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:VAN1(YML115C)|FD-Score:3.76|P-value:8.35E-5||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:YAP1(YML007W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR114C(YDR114C_p)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR220C(YDR220C_d)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YEL045C(YEL045C_d)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Putative protein of unknown function Gene:YGR160W(YGR160W_d)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR018W(YJR018W_d)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL044W(YKL044W_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLR149C(YLR149C_p)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR279W(YLR279W_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML007C-A(YML007C-A_p)|FD-Score:5.47|P-value:2.23E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YNG1(YOR064C)|FD-Score:-3.74|P-value:9.08E-5||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YOR316C-A(YOR316C-A_p)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL062W(YPL062W_d)|FD-Score:6.06|P-value:6.97E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR064W(YPR064W_d)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR092W(YPR092W_d)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZUO1(YGR285C)|FD-Score:8.02|P-value:5.47E-16||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p Gene:ADA2(YDR448W)|FD-Score:4.81|P-value:7.72E-7||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:AEP2(YMR282C)|FD-Score:5.81|P-value:3.11E-9||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AHC1(YOR023C)|FD-Score:3.3|P-value:4.79E-4||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:APL5(YPL195W)|FD-Score:4.83|P-value:6.66E-7||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:APN1(YKL114C)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:APQ13(YJL075C_d)|FD-Score:4.38|P-value:5.96E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene NET1; null mutant is sensitive to sorbate Gene:ARG2(YJL071W)|FD-Score:4|P-value:3.22E-5||SGD DESC:Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p Gene:ARP1(YHR129C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:ATG16(YMR159C)|FD-Score:5.54|P-value:1.54E-8||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATP15(YPL271W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:CWH43(YCR017C)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DNF1(YER166W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DUF1(YOL087C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EFG1(YGR271C-A)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ELM1(YKL048C)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring Gene:ERG2(YMR202W)|FD-Score:14.6|P-value:1.88E-48||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG3(YLR056W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ERG4(YGL012W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERG5(YMR015C)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV14(YGL054C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ESC1(YMR219W)|FD-Score:10.7|P-value:3.01E-27||SGD DESC:Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p Gene:EXG2(YDR261C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FLD1(YLR404W)|FD-Score:7.58|P-value:1.71E-14||SGD DESC:Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy Gene:FTR1(YER145C)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress Gene:GCS1(YDL226C)|FD-Score:-3.92|P-value:4.41E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GEP3(YOR205C)|FD-Score:5.8|P-value:3.40E-9||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GLO3(YER122C)|FD-Score:3.91|P-value:4.54E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p Gene:GYP6(YJL044C)|FD-Score:-4.85|P-value:6.32E-7||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HMI1(YOL095C)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:IMP2'(YIL154C)|FD-Score:9.19|P-value:1.90E-20||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:INP51(YIL002C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:IRS4(YKR019C)|FD-Score:5.04|P-value:2.29E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KNS1(YLL019C)|FD-Score:-4.12|P-value:1.90E-5||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LPP1(YDR503C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA Gene:LRG1(YDL240W)|FD-Score:-4.52|P-value:3.08E-6||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MBP1(YDL056W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes Gene:MEF1(YLR069C)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MPC54(YOR177C)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate Gene:MRF1(YGL143C)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Mitochondrial translation release factor, involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability Gene:MRP21(YBL090W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL16(YBL038W)|FD-Score:7.25|P-value:2.01E-13||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL27(YBR282W)|FD-Score:5.92|P-value:1.64E-9||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL32(YCR003W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MRPL4(YLR439W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPS12(YNR036C)|FD-Score:3.99|P-value:3.24E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MSS116(YDR194C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MTQ2(YDR140W)|FD-Score:5.22|P-value:8.85E-8||SGD DESC:S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC Gene:NOT3(YIL038C)|FD-Score:6.2|P-value:2.89E-10||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OSH2(YDL019C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PIM1(YBL022C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PKP2(YGL059W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PML39(YML107C)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:POP2(YNR052C)|FD-Score:7.62|P-value:1.27E-14||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:PTC1(YDL006W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:QCR7(YDR529C)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RML2(YEL050C)|FD-Score:4.1|P-value:2.02E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:RSC1(YGR056W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RSM18(YER050C)|FD-Score:4.41|P-value:5.26E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM27(YGR215W)|FD-Score:4.87|P-value:5.48E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:SAC1(YKL212W)|FD-Score:6|P-value:1.01E-9||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SEC22(YLR268W)|FD-Score:5.81|P-value:3.13E-9||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SFB3(YHR098C)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb2p Gene:SKM1(YOL113W)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Member of the PAK family of serine/threonine protein kinases with similarity to Ste20p and Cla4p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth Gene:SLA1(YBL007C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains Gene:SLS1(YLR139C)|FD-Score:4.61|P-value:2.05E-6||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SSN2(YDR443C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:SSN3(YPL042C)|FD-Score:4.02|P-value:2.86E-5||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SUV3(YPL029W)|FD-Score:9.2|P-value:1.81E-20||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:TAN1(YGL232W)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TGL2(YDR058C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli Gene:TMA46(YOR091W)|FD-Score:7.73|P-value:5.24E-15||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRK1(YJL129C)|FD-Score:5.35|P-value:4.28E-8||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:VAN1(YML115C)|FD-Score:3.76|P-value:8.35E-5||SGD DESC:Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant Gene:YAP1(YML007W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YDR114C(YDR114C_p)|FD-Score:4.47|P-value:3.94E-6||SGD DESC:Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium Gene:YDR220C(YDR220C_d)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein Gene:YEL045C(YEL045C_d)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGL188C-A(YGL188C-A_p)|FD-Score:3.2|P-value:6.88E-4||SGD DESC:Putative protein of unknown function Gene:YGR160W(YGR160W_d)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR018W(YJR018W_d)|FD-Score:4.08|P-value:2.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL044W(YKL044W_p)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLR149C(YLR149C_p)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR279W(YLR279W_d)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML007C-A(YML007C-A_p)|FD-Score:5.47|P-value:2.23E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YNG1(YOR064C)|FD-Score:-3.74|P-value:9.08E-5||SGD DESC:Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1 Gene:YOR316C-A(YOR316C-A_p)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL062W(YPL062W_d)|FD-Score:6.06|P-value:6.97E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPR064W(YPR064W_d)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR092W(YPR092W_d)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZUO1(YGR285C)|FD-Score:8.02|P-value:5.47E-16||SGD DESC:Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR340C6.066.69E-101.26RPA43RNA polymerase I subunit A43
YGL093W4.807.82E-70.26SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YER133W4.542.82E-60.95GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDL029W3.591.67E-40.57ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YKL165C3.020.001270.01MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YDR167W3.010.001300.08TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YMR290C2.930.001680.05HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
YBR140C2.890.001950.01IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YDR145W2.880.002000.00TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YNL007C2.880.002020.10SIS1Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress
YLR026C2.780.002730.10SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YIL083C2.680.003720.01CAB2Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
YPL028W2.660.003870.03ERG10Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis
YOR157C2.630.004230.00PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YPR025C2.630.004250.08CCL1Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR202W14.601.88E-48ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YMR219W10.703.01E-27ESC1Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p
YPL029W9.201.81E-20SUV3ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron
YIL154C9.191.90E-20IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YGR285C8.025.47E-16ZUO1Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
YOR091W7.735.24E-15TMA46Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
YNR052C7.621.27E-14POP2RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation
YLR404W7.581.71E-14FLD1Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy
YBL038W7.252.01E-13MRPL16Mitochondrial ribosomal protein of the large subunit
YIL038C6.202.89E-10NOT3Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
YPL062W_d6.066.97E-10YPL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation
YKL212W6.001.01E-9SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YBR282W5.921.64E-9MRPL27Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP
YMR282C5.813.11E-9AEP2Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
YLR268W5.813.13E-9SEC22R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog

GO enrichment analysis for SGTC_1624
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4154.79E-244SGTC_1623st003709 20.3 μMTimTec (Natural product derivative library)161926180.774648calcium & mitochondrial duress
0.4053.93E-232SGTC_1622st003707 19.4 μMTimTec (Natural product derivative library)101408990.597403calcium & mitochondrial duress
0.3766.81E-197SGTC_1627st003713 25.6 μMTimTec (Natural product derivative library)161950700.863636calcium & mitochondrial duress
0.3102.86E-131SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.103093ERG2
0.2871.37E-112SGTC_21445628481 200.0 μMChembridge (Fragment library)28183990.0804598ERG2
0.2852.18E-110SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.119565ERG2
0.2781.02E-104SGTC_5820987-0078 156.0 μMChemDiv (Drug-like library)16257650.105263calcium & mitochondrial duress
0.2646.64E-95SGTC_400oxethazaine 37.5 μMMiscellaneous46210.113636calcium & mitochondrial duress
0.2552.70E-88SGTC_2523spermine 47.7 μMMicrosource (Natural product library)11030.0135135calcium & mitochondrial duress
0.2495.59E-84SGTC_1974544-0043 41.6 μMChemDiv (Drug-like library)8965230.131868endomembrane recycling
0.2481.08E-83SGTC_1621st003705 32.4 μMTimTec (Natural product derivative library)242070320.469136calcium & mitochondrial duress
0.2422.74E-79SGTC_2720fluspirilene 36.9 μMMiscellaneous33960.0882353ERG2
0.2423.18E-79SGTC_2533agelasine 13.7 μMMicrosource (Natural product library)166677450.104348ERG2
0.2359.16E-75SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.104167ERG2
0.2325.42E-73SGTC_1871488-2104 32.7 μMChemDiv (Drug-like library)28528590.15534calcium & mitochondrial duress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1627st00371325.6 μM0.86363616195070TimTec (Natural product derivative library)501.057383.90205calcium & mitochondrial duress
SGTC_1626st0037121.76 μM0.85074624207035TimTec (Natural product derivative library)505.476464.58304
SGTC_1623st00370920.3 μM0.77464816192618TimTec (Natural product derivative library)485.057984.40504calcium & mitochondrial duress
SGTC_1625st00371130.1 μM0.75714324207034TimTec (Natural product derivative library)501.057383.90205calcium & mitochondrial duress
SGTC_1622st00370719.4 μM0.59740310140899TimTec (Natural product derivative library)485.057984.40504calcium & mitochondrial duress
SGTC_1620st00370416.5 μM0.5584422834414TimTec (Natural product derivative library)519.901484.83203
SGTC_1621st00370532.4 μM0.46913624207032TimTec (Natural product derivative library)455.0324.5703calcium & mitochondrial duress
SGTC_15034585-001261.3 μM0.3452382922052ChemDiv (Drug-like library)338.464943.81214
SGTC_1812st0520574.33 μM0.3033713645418TimTec (Natural product derivative library)422.664224.01203calcium & mitochondrial duress
SGTC_3021908765849.47 μM0.25316525237042Chembridge (Drug-like library)275.38593.58802