st004252

N-cyclopentyl-2-oxochromene-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1628
Screen concentration 77.7 μM
Source TimTec (Natural product derivative library)
PubChem CID 692968
SMILES C1CCC(C1)NC(=O)C2=CC3=CC=CC=C3OC2=O
Standardized SMILES O=C(NC1CCCC1)C2=Cc3ccccc3OC2=O
Molecular weight 257.2845
ALogP 2.47
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.62
% growth inhibition (Hom. pool) 7.83


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 692968
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:-4.05|P-value:2.59E-5|Clearance:0||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:AME1(YBR211C)|FD-Score:3.4|P-value:3.40E-4|Clearance:0||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:COG2(YGR120C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.12||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:GRS1(YBR121C)|FD-Score:3.1|P-value:9.75E-4|Clearance:0.02||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:KOG1(YHR186C)|FD-Score:-3.98|P-value:3.47E-5|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LSG1(YGL099W)|FD-Score:9.38|P-value:3.43E-21|Clearance:3.77||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NOP7(YGR103W)|FD-Score:3.28|P-value:5.27E-4|Clearance:0.18||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:RPS2(YGL123W)|FD-Score:-3.11|P-value:9.38E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RRP42(YDL111C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.06||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SAD1(YFR005C)|FD-Score:4.97|P-value:3.29E-7|Clearance:0.07||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SFI1(YLL003W)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.04||SGD DESC:Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C Gene:SHQ1(YIL104C)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.06||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SLD2(YKL108W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:SPP382(YLR424W)|FD-Score:-4.89|P-value:5.12E-7|Clearance:0||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:TFB3(YDR460W)|FD-Score:-4.49|P-value:3.62E-6|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TFG1(YGR186W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.02||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:TRS120(YDR407C)|FD-Score:4.91|P-value:4.62E-7|Clearance:1.32||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YPL238C(YPL238C_d)|FD-Score:5.6|P-value:1.06E-8|Clearance:0.63||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:AFG2(YLR397C)|FD-Score:-4.05|P-value:2.59E-5|Clearance:0||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:AME1(YBR211C)|FD-Score:3.4|P-value:3.40E-4|Clearance:0||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:COG2(YGR120C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.12||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:GRS1(YBR121C)|FD-Score:3.1|P-value:9.75E-4|Clearance:0.02||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:KOG1(YHR186C)|FD-Score:-3.98|P-value:3.47E-5|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:LSG1(YGL099W)|FD-Score:9.38|P-value:3.43E-21|Clearance:3.77||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:NOP7(YGR103W)|FD-Score:3.28|P-value:5.27E-4|Clearance:0.18||SGD DESC:Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G<sub>0</sub> and the initiation of cell proliferation Gene:RPS2(YGL123W)|FD-Score:-3.11|P-value:9.38E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RRP42(YDL111C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.06||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SAD1(YFR005C)|FD-Score:4.97|P-value:3.29E-7|Clearance:0.07||SGD DESC:Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle Gene:SFI1(YLL003W)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.04||SGD DESC:Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C Gene:SHQ1(YIL104C)|FD-Score:3.34|P-value:4.22E-4|Clearance:0.06||SGD DESC:Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones Gene:SLD2(YKL108W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:SPP382(YLR424W)|FD-Score:-4.89|P-value:5.12E-7|Clearance:0||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:TFB3(YDR460W)|FD-Score:-4.49|P-value:3.62E-6|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TFG1(YGR186W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.02||SGD DESC:TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 Gene:TRS120(YDR407C)|FD-Score:4.91|P-value:4.62E-7|Clearance:1.32||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:YPL238C(YPL238C_d)|FD-Score:5.6|P-value:1.06E-8|Clearance:0.63||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 692968
Download HOP data (tab-delimited text)  (excel)
Gene:AAT1(YKL106W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis Gene:ADE16(YLR028C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AIM6(YDL237W)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ALY2(YJL084C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:ARC18(YLR370C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG12(YBR217W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:BMH1(YER177W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BNR1(YIL159W)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BSC1(YDL037C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:COX14(YML129C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:COX17(YLL009C)|FD-Score:6.33|P-value:1.21E-10||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CSR2(YPR030W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CTM1(YHR109W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:DOT1(YDR440W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:EMC4(YGL231C)|FD-Score:4.39|P-value:5.68E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:FAR11(YNL127W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FAU1(YER183C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FIS1(YIL065C)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:GND1(YHR183W)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HEL2(YDR266C)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor Gene:IRC8(YJL051W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc Gene:IRS4(YKR019C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISR1(YPR106W)|FD-Score:-3.73|P-value:9.45E-5||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KAP120(YPL125W)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KIN2(YLR096W)|FD-Score:5|P-value:2.84E-7||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:KTR2(YKR061W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:-3.95|P-value:3.97E-5||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM6(YDR378C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LSP1(YPL004C)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family Gene:MFT1(YML062C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MIC17(YMR002W)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MLH2(YLR035C)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MNT2(YGL257C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MSO1(YNR049C)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NAM7(YMR080C)|FD-Score:5.01|P-value:2.67E-7||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NDT80(YHR124W)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NIF3(YGL221C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:OPI11(YPR044C_d)|FD-Score:5.22|P-value:8.99E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:OPI9(YLR338W_d)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OPY2(YPR075C)|FD-Score:4.99|P-value:3.07E-7||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:OXP1(YKL215C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PEP3(YLR148W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PKR1(YMR123W)|FD-Score:-3.86|P-value:5.60E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PTC5(YOR090C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RED1(YLR263W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RPS6B(YBR181C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication Gene:RTR1(YER139C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SPT2(YER161C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SRB5(YGR104C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:STE23(YLR389C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:STP1(YDR463W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SUR2(YDR297W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TPM2(YIL138C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.8|P-value:3.31E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TVP15(YDR100W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:ULI1(YFR026C)|FD-Score:6|P-value:9.99E-10||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:VFA1(YER128W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:YAP5(YIR018W)|FD-Score:4.39|P-value:5.56E-6||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBL095W(YBL095W_p)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR064C(YCR064C_d)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL041W(YDL041W_d)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR433W(YDR433W_d)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL101W(YGL101W_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p Gene:YGR226C(YGR226C_d)|FD-Score:5.38|P-value:3.71E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YIA6(YIL006W)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL100W(YIL100W_d)|FD-Score:4.46|P-value:4.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YJL007C(YJL007C_d)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR098C(YJR098C_p)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YJU3(YKL094W)|FD-Score:5.97|P-value:1.17E-9||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YLR194C(YLR194C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR460C(YLR460C_p)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML122C(YML122C_d)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR253C(YMR253C_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YPR003C(YPR003C_p)|FD-Score:-4.58|P-value:2.33E-6||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR022C(YPR022C_p)|FD-Score:4.41|P-value:5.22E-6||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR108W-A(YPR108W-A_p)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:AAT1(YKL106W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis Gene:ADE16(YLR028C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:AIM6(YDL237W)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ALY2(YJL084C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; phosphorylated by Npr1p and also by cyclin-CDK complex Pcl7p-Pho85p; promotes endocytosis of plasma membrane proteins; ALY2 has a paralog, ALY1, that arose from the whole genome duplication Gene:ARC18(YLR370C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATG12(YBR217W)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Conserved ubiquitin-like modifier involved in autophagy and the Cvt pathway; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg12p-Atg5p also forms a complex with Atg16p that is required for autophagosome formation Gene:BMH1(YER177W)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BNR1(YIL159W)|FD-Score:3.14|P-value:8.31E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1 Gene:BSC1(YDL037C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:COX14(YML129C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:COX17(YLL009C)|FD-Score:6.33|P-value:1.21E-10||SGD DESC:Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CSR2(YPR030W)|FD-Score:4.13|P-value:1.81E-5||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CTM1(YHR109W)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:DOT1(YDR440W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:EMC4(YGL231C)|FD-Score:4.39|P-value:5.68E-6||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:FAR11(YNL127W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:FAU1(YER183C)|FD-Score:4.2|P-value:1.35E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FIS1(YIL065C)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation Gene:GND1(YHR183W)|FD-Score:-3.3|P-value:4.92E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HAA1(YPR008W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HEL2(YDR266C)|FD-Score:3.86|P-value:5.77E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor Gene:IRC8(YJL051W)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc Gene:IRS4(YKR019C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISR1(YPR106W)|FD-Score:-3.73|P-value:9.45E-5||SGD DESC:Predicted protein kinase, overexpression causes sensitivity to staurosporine, which is a potent inhibitor of protein kinase C Gene:KAP120(YPL125W)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KIN2(YLR096W)|FD-Score:5|P-value:2.84E-7||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:KTR2(YKR061W)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:-3.95|P-value:3.97E-5||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LSM6(YDR378C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LSP1(YPL004C)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Primary component of eisosomes, which are large immobile patch structures at the cell cortex associated with endocytosis, along with Pil1p and Sur7p; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family Gene:MFT1(YML062C)|FD-Score:3.26|P-value:5.61E-4||SGD DESC:Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance Gene:MIC17(YMR002W)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MLH2(YLR035C)|FD-Score:3.74|P-value:9.19E-5||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MNT2(YGL257C)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Mannosyltransferase involved in adding the 4th and 5th mannose residues of O-linked glycans Gene:MSO1(YNR049C)|FD-Score:-3.1|P-value:9.68E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NAM7(YMR080C)|FD-Score:5.01|P-value:2.67E-7||SGD DESC:ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress Gene:NDT80(YHR124W)|FD-Score:-3.14|P-value:8.55E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NIF3(YGL221C)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:OPI11(YPR044C_d)|FD-Score:5.22|P-value:8.99E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:OPI9(YLR338W_d)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OPY2(YPR075C)|FD-Score:4.99|P-value:3.07E-7||SGD DESC:Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress Gene:OXP1(YKL215C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:PEP3(YLR148W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PKR1(YMR123W)|FD-Score:-3.86|P-value:5.60E-5||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PTC5(YOR090C)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RED1(YLR263W)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Protein component of the synaptonemal complex axial elements, involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; interacts with Hop1p, Mec3p and Ddc1p Gene:RPS6B(YBR181C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication Gene:RTR1(YER139C)|FD-Score:-3.43|P-value:2.97E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SPT2(YER161C)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SRB5(YGR104C)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:STE23(YLR389C)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; expressed in both haploids and diploids; member of the insulin-degrading enzyme family Gene:STP1(YDR463W)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SUR2(YDR297W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:TPM2(YIL138C)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.8|P-value:3.31E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TVP15(YDR100W)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p Gene:ULI1(YFR026C)|FD-Score:6|P-value:9.99E-10||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:VFA1(YER128W)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:YAP5(YIR018W)|FD-Score:4.39|P-value:5.56E-6||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBL095W(YBL095W_p)|FD-Score:3.12|P-value:9.03E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YCR064C(YCR064C_d)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDL041W(YDL041W_d)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDR433W(YDR433W_d)|FD-Score:-3.09|P-value:9.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:-4.15|P-value:1.63E-5||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGL101W(YGL101W_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; non-essential gene with similarity to YBR242W; interacts with the DNA helicase Hpr5p Gene:YGR226C(YGR226C_d)|FD-Score:5.38|P-value:3.71E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YIA6(YIL006W)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Mitochondrial NAD+ transporter, involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs Gene:YIL100W(YIL100W_d)|FD-Score:4.46|P-value:4.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YJL007C(YJL007C_d)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR098C(YJR098C_p)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YJU3(YKL094W)|FD-Score:5.97|P-value:1.17E-9||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YLR194C(YLR194C)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR460C(YLR460C_p)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YML122C(YML122C_d)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR253C(YMR253C_p)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YPR003C(YPR003C_p)|FD-Score:-4.58|P-value:2.33E-6||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR022C(YPR022C_p)|FD-Score:4.41|P-value:5.22E-6||SGD DESC:Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YPR108W-A(YPR108W-A_p)|FD-Score:-3.37|P-value:3.76E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W9.383.43E-213.77LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YPL238C_d5.601.06E-80.63YPL238C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
YFR005C4.973.29E-70.07SAD1Conserved zinc-finger domain protein involved in pre-mRNA splicing, required for assembly of U4 snRNA into the U4/U6 particle
YDR407C4.914.62E-71.32TRS120One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YGR186W3.591.68E-40.02TFG1TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
YGR120C3.561.85E-40.12COG2Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLL003W3.442.90E-40.04SFI1Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C
YBR211C3.403.40E-40.00AME1Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
YDL111C3.393.45E-40.06RRP42Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
YIL104C3.344.22E-40.06SHQ1Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
YGR103W3.285.27E-40.18NOP7Component of several different pre-ribosomal particles; forms a complex with Ytm1p and Erb1p that is required for maturation of the large ribosomal subunit; required for exit from G0 and the initiation of cell proliferation
YBR121C3.109.75E-40.02GRS1Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
YGR245C3.080.001040.01SDA1Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
YJL041W3.070.001070.04NSP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress
YBR011C3.030.001210.09IPP1Cytoplasmic inorganic pyrophosphatase (PPase), homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLL009C6.331.21E-10COX17Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
YFR026C6.009.99E-10ULI1Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response
YKL094W5.971.17E-9YJU3Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family
YKL211C5.803.31E-9TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGR226C_d5.383.71E-8YGR226C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
YPR044C_d5.228.99E-8OPI11_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO
YMR080C5.012.67E-7NAM7ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
YLR096W5.002.84E-7KIN2Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p
YPR075C4.993.07E-7OPY2Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
YIL100W_d4.464.15E-6YIL100W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A
YPR022C_p4.415.22E-6YPR022C_pPutative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YIR018W4.395.56E-6YAP5Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication
YGL231C4.395.68E-6EMC4Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85
YKL215C4.241.13E-5OXP15-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
YER183C4.201.35E-5FAU15,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis

GO enrichment analysis for SGTC_1628
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1661.04E-37SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.107692
0.1482.79E-30SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)3015189NA
0.1474.28E-30SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.147059
0.1433.00E-28SGTC_1636st007707 68.9 μMTimTec (Natural product derivative library)4030510.130435
0.1372.84E-26SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0535714
0.1352.84E-25SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.071428660S ribosome export
0.1337.36E-25SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0681818
0.1316.38E-24SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.15853760S ribosome export
0.1278.49E-23SGTC_24725377652 49.4 μMMiscellaneous16109030.125TRP & mitochondrial translation
0.1271.33E-22SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.12903260S ribosome export
0.1254.21E-22SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.218182
0.1217.81E-21SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.106061
0.1219.71E-21SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.077922160S ribosome export
0.1171.38E-19SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.144737
0.1172.56E-19SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.177419

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22047233102200 μM0.5416672172480Chembridge (Fragment library)229.231341.6841360S ribosome export
SGTC_1971st06942963.5 μM0.4905661246522TimTec (Natural product derivative library)280.278082.5191460S ribosome export
SGTC_8810833-0569170 μM0.4807695418700ChemDiv (Drug-like library)306.315362.68414
SGTC_1964st07691461 μM0.41379324208661TimTec (Natural product derivative library)327.761663.82714RPP1 & pyrimidine depletion
SGTC_1821st05346565.6 μM0.411765675086TimTec (Natural product derivative library)282.090121.81613
SGTC_10410206-0046107 μM0.40384650494ChemDiv (Drug-like library)258.315621.79624
SGTC_1914st06236772.6 μM0.375978205TimTec (Natural product derivative library)275.299781.98214
SGTC_22117117706200 μM0.361702822771Chembridge (Fragment library)177.199841.28522
SGTC_1701st02863840.4 μM0.3611113490878TimTec (Natural product derivative library)494.546123.04638
SGTC_9041141-0088396 μM0.3606561378119ChemDiv (Drug-like library)371.385384.14614