st007924

5-chloro-7-[(4-methylpiperidin-1-yl)methyl]quinolin-8-ol

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1637
Screen concentration 17.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 683336
SMILES CC1CCN(CC1)CC2=CC(=C3C=CC=NC3=C2O)Cl
Standardized SMILES CC1CCN(Cc2cc(Cl)c3cccnc3c2O)CC1
Molecular weight 290.7879
ALogP 3.68
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.29
% growth inhibition (Hom. pool) 4.72


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 683336
Download HIP data (tab-delimited text)  (excel)
Gene:AOS1(YPR180W)|FD-Score:-3.26|P-value:5.61E-4|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:CWC23(YGL128C)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:DPB2(YPR175W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.17||SGD DESC:Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate Gene:ERG20(YJL167W)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:ESP1(YGR098C)|FD-Score:4.63|P-value:1.87E-6|Clearance:0.32||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GLN1(YPR035W)|FD-Score:-4.51|P-value:3.22E-6|Clearance:0||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LCB2(YDR062W)|FD-Score:-3.76|P-value:8.58E-5|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MCD4(YKL165C)|FD-Score:3.84|P-value:6.16E-5|Clearance:0.14||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MED7(YOL135C)|FD-Score:-4.65|P-value:1.69E-6|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MEX67(YPL169C)|FD-Score:3.94|P-value:4.05E-5|Clearance:0.1||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:POL30(YBR088C)|FD-Score:-3.19|P-value:7.08E-4|Clearance:0||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:RPS2(YGL123W)|FD-Score:-3.48|P-value:2.47E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPT5(YOR117W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.08||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RRP3(YHR065C)|FD-Score:-3.16|P-value:7.94E-4|Clearance:0||SGD DESC:Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity Gene:RSC4(YKR008W)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSE1(YML049C)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.05||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SNU23(YDL098C)|FD-Score:-5.39|P-value:3.49E-8|Clearance:0||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:SPC25(YER018C)|FD-Score:4.3|P-value:8.39E-6|Clearance:0.36||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:SPP382(YLR424W)|FD-Score:5.48|P-value:2.08E-8|Clearance:0.86||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:STH1(YIL126W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.27||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:THS1(YIL078W)|FD-Score:-3.65|P-value:1.31E-4|Clearance:0||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM50(YPL063W)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.01||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:TRS130(YMR218C)|FD-Score:-3.23|P-value:6.25E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:TSR1(YDL060W)|FD-Score:-4.47|P-value:3.98E-6|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YAH1(YPL252C)|FD-Score:-4.67|P-value:1.52E-6|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YDR187C(YDR187C_d)|FD-Score:3.11|P-value:9.40E-4|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:AOS1(YPR180W)|FD-Score:-3.26|P-value:5.61E-4|Clearance:0||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:CWC23(YGL128C)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:DPB2(YPR175W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.17||SGD DESC:Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate Gene:ERG20(YJL167W)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis Gene:ESP1(YGR098C)|FD-Score:4.63|P-value:1.87E-6|Clearance:0.32||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GLN1(YPR035W)|FD-Score:-4.51|P-value:3.22E-6|Clearance:0||SGD DESC:Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:LCB2(YDR062W)|FD-Score:-3.76|P-value:8.58E-5|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MCD4(YKL165C)|FD-Score:3.84|P-value:6.16E-5|Clearance:0.14||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MED7(YOL135C)|FD-Score:-4.65|P-value:1.69E-6|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MEX67(YPL169C)|FD-Score:3.94|P-value:4.05E-5|Clearance:0.1||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:POL30(YBR088C)|FD-Score:-3.19|P-value:7.08E-4|Clearance:0||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:RPS2(YGL123W)|FD-Score:-3.48|P-value:2.47E-4|Clearance:0||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPT5(YOR117W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.08||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RRP3(YHR065C)|FD-Score:-3.16|P-value:7.94E-4|Clearance:0||SGD DESC:Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity Gene:RSC4(YKR008W)|FD-Score:3.12|P-value:9.16E-4|Clearance:0.01||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSE1(YML049C)|FD-Score:3.45|P-value:2.81E-4|Clearance:0.05||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SNU23(YDL098C)|FD-Score:-5.39|P-value:3.49E-8|Clearance:0||SGD DESC:Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome Gene:SPC25(YER018C)|FD-Score:4.3|P-value:8.39E-6|Clearance:0.36||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:SPP382(YLR424W)|FD-Score:5.48|P-value:2.08E-8|Clearance:0.86||SGD DESC:Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation Gene:STH1(YIL126W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.27||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:THS1(YIL078W)|FD-Score:-3.65|P-value:1.31E-4|Clearance:0||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM50(YPL063W)|FD-Score:3.12|P-value:8.90E-4|Clearance:0.01||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:TRS130(YMR218C)|FD-Score:-3.23|P-value:6.25E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:TSR1(YDL060W)|FD-Score:-4.47|P-value:3.98E-6|Clearance:0||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:YAH1(YPL252C)|FD-Score:-4.67|P-value:1.52E-6|Clearance:0||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:YDR187C(YDR187C_d)|FD-Score:3.11|P-value:9.40E-4|Clearance:0.23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 683336
Download HOP data (tab-delimited text)  (excel)
Gene:ABM1(YJR108W)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ACO1(YLR304C)|FD-Score:4.8|P-value:7.74E-7||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AIM6(YDL237W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ALK2(YBL009W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:ALO1(YML086C)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:APE2(YKL157W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:ARO7(YPR060C)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARR1(YPR199C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ATG20(YDL113C)|FD-Score:-4.55|P-value:2.66E-6||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:AVT1(YJR001W)|FD-Score:5.52|P-value:1.70E-8||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BEM3(YPL115C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BLI1(YKL061W_p)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BNA5(YLR231C)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BUD30(YDL151C_d)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAM1(YPL048W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CBS2(YDR197W)|FD-Score:-4.06|P-value:2.50E-5||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:COQ8(YGL119W)|FD-Score:-4.36|P-value:6.60E-6||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:DAL7(YIR031C)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAN1(YJR150C)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DFM1(YDR411C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DOG1(YHR044C)|FD-Score:-3.13|P-value:8.66E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:DOT1(YDR440W)|FD-Score:-4.05|P-value:2.53E-5||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:FRD1(YEL047C)|FD-Score:-12.7|P-value:2.65E-37||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:FUI1(YBL042C)|FD-Score:6.32|P-value:1.34E-10||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:HAC1(YFL031W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HSL7(YBR133C)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IOC2(YLR095C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:ISU1(YPL135W)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:JEN1(YKL217W)|FD-Score:8.73|P-value:1.22E-18||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:LRG1(YDL240W)|FD-Score:5.33|P-value:4.92E-8||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:LYS4(YDR234W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MNN2(YBR015C)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRP13(YGR084C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL37(YBR268W)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NDJ1(YOL104C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:NUR1(YDL089W)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:ORM2(YLR350W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OSW1(YOR255W)|FD-Score:5.33|P-value:4.82E-8||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PEP1(YBL017C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments Gene:PET20(YPL159C)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PEX1(YKL197C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PPZ2(YDR436W)|FD-Score:-3.21|P-value:6.52E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRC1(YMR297W)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRO2(YOR323C)|FD-Score:10.9|P-value:5.89E-28||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PST1(YDR055W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PUT1(YLR142W)|FD-Score:-4.1|P-value:2.07E-5||SGD DESC:Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Gene:QCR8(YJL166W)|FD-Score:-3.79|P-value:7.61E-5||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:RBD2(YPL246C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p Gene:RBG2(YGR173W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RDS1(YCR106W)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RNH70(YGR276C)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPS29A(YLR388W)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SAC7(YDR389W)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SEM1(YDR363W-A)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SHH4(YLR164W)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SLH1(YGR271W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SRB5(YGR104C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRY1(YKL218C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SUL2(YLR092W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:TGL3(YMR313C)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:THI74(YDR438W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TMA108(YIL137C)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TOD6(YBL054W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAC7(YNL054W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VCX1(YDL128W)|FD-Score:-4.27|P-value:9.57E-6||SGD DESC:Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter Gene:YAF9(YNL107W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YCH1(YGR203W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YCK1(YHR135C)|FD-Score:4.77|P-value:9.30E-7||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCL076W(YCL076W_d)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCT1(YLL055W)|FD-Score:8.23|P-value:9.35E-17||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:-4.37|P-value:6.29E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YEL010W(YEL010W_d)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Putative protein of unknown function Gene:YER188W(YER188W_d)|FD-Score:-4.38|P-value:6.01E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YFR056C(YFR056C_d)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YGL034C(YGL034C_d)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR175W-A(YHR175W-A_p)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL024C(YIL024C_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YIL058W(YIL058W_d)|FD-Score:-6.34|P-value:1.12E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL134C-A(YIL134C-A_p)|FD-Score:-4.61|P-value:2.05E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIR042C(YIR042C_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YJU3(YKL094W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL123W(YKL123W_d)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YKR032W(YKR032W_d)|FD-Score:-5.3|P-value:5.85E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR012C(YLR012C_p)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR194C(YLR194C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR297W(YLR297W_p)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YML122C(YML122C_d)|FD-Score:-7.2|P-value:3.10E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR196W(YMR196W_p)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL010W(YNL010W_p)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL035C(YNL035C_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL193W(YNL193W_p)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YNL295W(YNL295W_p)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Putative protein of unknown function Gene:YOL114C(YOL114C_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOL118C(YOL118C_d)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR1(YGR281W)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPS1(YLR120C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPT32(YGL210W)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication Gene:YPT35(YHR105W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:ABM1(YJR108W)|FD-Score:-3.23|P-value:6.27E-4||SGD DESC:Protein of unknown function, required for normal microtubule organization Gene:ACO1(YLR304C)|FD-Score:4.8|P-value:7.74E-7||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AIM6(YDL237W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ALK2(YBL009W)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:ALO1(YML086C)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:D-Arabinono-1,4-lactone oxidase, catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress Gene:APE2(YKL157W)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:ARO7(YPR060C)|FD-Score:3.78|P-value:7.73E-5||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARR1(YPR199C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Transcriptional activator of the basic leucine zipper (bZIP) family, required for transcription of genes involved in resistance to arsenic compounds Gene:ATG20(YDL113C)|FD-Score:-4.55|P-value:2.66E-6||SGD DESC:Sorting nexin family member required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate Gene:AVT1(YJR001W)|FD-Score:5.52|P-value:1.70E-8||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BEM3(YPL115C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly Gene:BLI1(YKL061W_p)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome Gene:BNA5(YLR231C)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BUD30(YDL151C_d)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAM1(YPL048W)|FD-Score:3.35|P-value:4.06E-4||SGD DESC:Nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids; has similarity to translational cofactor EF-1 gamma Gene:CBS2(YDR197W)|FD-Score:-4.06|P-value:2.50E-5||SGD DESC:Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader Gene:COQ8(YGL119W)|FD-Score:-4.36|P-value:6.60E-6||SGD DESC:Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication Gene:DAL7(YIR031C)|FD-Score:3.84|P-value:6.19E-5||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DAN1(YJR150C)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DFM1(YDR411C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Endoplasmic reticulum (ER) localized protein involved in ER-associated protein degradation (ERAD), ER stress and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p Gene:DOG1(YHR044C)|FD-Score:-3.13|P-value:8.66E-4||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; paralogous to DOG2; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified Gene:DOT1(YDR440W)|FD-Score:-4.05|P-value:2.53E-5||SGD DESC:Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response Gene:FRD1(YEL047C)|FD-Score:-12.7|P-value:2.65E-37||SGD DESC:Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication Gene:FUI1(YBL042C)|FD-Score:6.32|P-value:1.34E-10||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:HAC1(YFL031W)|FD-Score:3.4|P-value:3.31E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HSL7(YBR133C)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IOC2(YLR095C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:ISU1(YPL135W)|FD-Score:-4.22|P-value:1.22E-5||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:JEN1(YKL217W)|FD-Score:8.73|P-value:1.22E-18||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:LRG1(YDL240W)|FD-Score:5.33|P-value:4.92E-8||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:LYS4(YDR234W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway Gene:MNN2(YBR015C)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MRP13(YGR084C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL37(YBR268W)|FD-Score:-3.31|P-value:4.68E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSB1(YOR188W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:NDJ1(YOL104C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Meiosis-specific telomere protein, required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement Gene:NUR1(YDL089W)|FD-Score:3.72|P-value:9.87E-5||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:ORM2(YLR350W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress Gene:OSW1(YOR255W)|FD-Score:5.33|P-value:4.82E-8||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PEP1(YBL017C)|FD-Score:-3.11|P-value:9.39E-4||SGD DESC:Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments Gene:PET20(YPL159C)|FD-Score:3.83|P-value:6.30E-5||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PEX1(YKL197C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PPZ2(YDR436W)|FD-Score:-3.21|P-value:6.52E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRC1(YMR297W)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRO2(YOR323C)|FD-Score:10.9|P-value:5.89E-28||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PST1(YDR055W)|FD-Score:4.24|P-value:1.12E-5||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PUT1(YLR142W)|FD-Score:-4.1|P-value:2.07E-5||SGD DESC:Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source Gene:QCR8(YJL166W)|FD-Score:-3.79|P-value:7.61E-5||SGD DESC:Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p Gene:RBD2(YPL246C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Possible rhomboid protease, has similarity to eukaryotic rhomboid proteases including Pcp1p Gene:RBG2(YGR173W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RDS1(YCR106W)|FD-Score:-3.4|P-value:3.34E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RNH70(YGR276C)|FD-Score:4.27|P-value:9.69E-6||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPS29A(YLR388W)|FD-Score:5.46|P-value:2.38E-8||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication Gene:SAC7(YDR389W)|FD-Score:-3.09|P-value:9.98E-4||SGD DESC:GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate Gene:SEM1(YDR363W-A)|FD-Score:5.15|P-value:1.30E-7||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SHH4(YLR164W)|FD-Score:-4.02|P-value:2.86E-5||SGD DESC:Mitochondrial inner membrane protein of unknown function; similar to Tim18p and Sdh4p; a fraction copurifies with Sdh3p, but Shh4p is neither a stoichiometric subunit of succinate dehydrogenase nor of the TIM22 translocase; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner Gene:SLH1(YGR271W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses Gene:SRB5(YGR104C)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRY1(YKL218C)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SUL2(YLR092W)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:TGL3(YMR313C)|FD-Score:3.88|P-value:5.14E-5||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:THI74(YDR438W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication Gene:TMA108(YIL137C)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TOD6(YBL054W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:URM1(YIL008W)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAC7(YNL054W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VCX1(YDL128W)|FD-Score:-4.27|P-value:9.57E-6||SGD DESC:Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity, involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter Gene:YAF9(YNL107W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YCH1(YGR203W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YCK1(YHR135C)|FD-Score:4.77|P-value:9.30E-7||SGD DESC:Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCL076W(YCL076W_d)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCT1(YLL055W)|FD-Score:8.23|P-value:9.35E-17||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:-4.37|P-value:6.29E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YEL010W(YEL010W_d)|FD-Score:3.93|P-value:4.27E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Putative protein of unknown function Gene:YER188W(YER188W_d)|FD-Score:-4.38|P-value:6.01E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YFR056C(YFR056C_d)|FD-Score:-3.57|P-value:1.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YGL034C(YGL034C_d)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR175W-A(YHR175W-A_p)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL024C(YIL024C_p)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p Gene:YIL058W(YIL058W_d)|FD-Score:-6.34|P-value:1.12E-10||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL134C-A(YIL134C-A_p)|FD-Score:-4.61|P-value:2.05E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIR042C(YIR042C_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YJU3(YKL094W)|FD-Score:-3.56|P-value:1.86E-4||SGD DESC:Monoglyceride lipase (MGL), functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family Gene:YKL123W(YKL123W_d)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4 Gene:YKR032W(YKR032W_d)|FD-Score:-5.3|P-value:5.85E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR012C(YLR012C_p)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Putative protein of unknown function; YLR012C is not an essential gene Gene:YLR194C(YLR194C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YLR297W(YLR297W_p)|FD-Score:-3.39|P-value:3.54E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication Gene:YML122C(YML122C_d)|FD-Score:-7.2|P-value:3.10E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR196W(YMR196W_p)|FD-Score:3.78|P-value:7.74E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YNL010W(YNL010W_p)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Putative protein of unknown function with similarity to phosphoserine phosphatases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant shows an increase in glycogen accumulation Gene:YNL035C(YNL035C_p)|FD-Score:3.52|P-value:2.16E-4||SGD DESC:Protein of unknown function; contains WD-40 domains; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YNL035C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YNL193W(YNL193W_p)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YNL295W(YNL295W_p)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Putative protein of unknown function Gene:YOL114C(YOL114C_p)|FD-Score:3.52|P-value:2.13E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOL118C(YOL118C_d)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR1(YGR281W)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions including oligomycin; similar to human cystic fibrosis transmembrane receptor (CFTR) Gene:YPS1(YLR120C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YPT32(YGL210W)|FD-Score:-3.11|P-value:9.48E-4||SGD DESC:Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication Gene:YPT35(YHR105W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR424W5.482.08E-80.86SPP382Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
YGR098C4.631.87E-60.32ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YER018C4.308.39E-60.36SPC25Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YPL169C3.944.05E-50.10MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YKL165C3.846.16E-50.14MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YPR175W3.701.08E-40.17DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YOR117W3.532.07E-40.08RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YML049C3.452.81E-40.05RSE1Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
YIL126W3.403.42E-40.27STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YPL063W3.128.90E-40.01TIM50Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
YKR008W3.129.16E-40.01RSC4Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
YDR187C_d3.119.40E-40.23YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YKL013C2.880.002000.01ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YKR038C2.870.002080.01KAE1Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription
YNL088W2.850.002170.18TOP2Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR323C10.905.89E-28PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YKL217W8.731.22E-18JEN1Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose
YLL055W8.239.35E-17YCT1High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene
YBL042C6.321.34E-10FUI1High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
YJR001W5.521.70E-8AVT1Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YLR388W5.462.38E-8RPS29AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication
YOR255W5.334.82E-8OSW1Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p
YDL240W5.334.92E-8LRG1Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis
YDR363W-A5.151.30E-7SEM1Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress
YBR015C5.151.31E-7MNN2Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
YLR231C5.091.80E-7BNA5Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
YLR304C4.807.74E-7ACO1Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
YHR135C4.779.30E-7YCK1Palmitoylated plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; YCK1 has a paralog, YCK2, that arose from the whole genome duplication
YKL123W_d4.671.54E-6YKL123W_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene SSH4
YGR276C4.279.69E-6RNH703'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts

GO enrichment analysis for SGTC_1637
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0831.76E-10SGTC_468gf-109203x 121.0 μMICCB bioactive library23960.0952381amide catabolism
0.0806.30E-10SGTC_2501sodium tetradecyl sulfate 13.4 μMMicrosource (Natural product library)236657720.027027
0.0782.42E-9SGTC_772k072-0172 35.3 μMChemDiv (Drug-like library)58619800.0779221
0.0672.91E-7SGTC_31109122132 49.5 μMChembridge (Drug-like library)9183170.130435redox potentiating
0.0663.34E-7SGTC_27847567673 72.7 μMChembridge (Drug-like library)34712470.557692iron homeostasis
0.0648.55E-7SGTC_21695-(ethoxymethyl)quinolin-8-ol 49.5 μMChembridge (Fragment library)2796210.310345
0.0649.50E-7SGTC_31759106517 49.5 μMChembridge (Drug-like library)414680730.123288iron homeostasis
0.0641.04E-6SGTC_7710250-0039 27.3 μMChemDiv (Drug-like library)30051150.0675676
0.0631.23E-6SGTC_29419056145 11.2 μMChembridge (Drug-like library)21195170.105263
0.0631.23E-6SGTC_13712421-0008 32.7 μMChemDiv (Drug-like library)28275520.0789474mitochondrial processes
0.0631.34E-6SGTC_21836420625 130.5 μMChembridge (Fragment library)28976190.0649351mitochondrial processes
0.0622.04E-6SGTC_20464110841 106.0 μMChembridge (Fragment library)441196300.1iron homeostasis
0.0622.23E-6SGTC_24855633444 17.8 μMMiscellaneous57242390.169014redox potentiating
0.0612.38E-6SGTC_1638st008370 60.6 μMTimTec (Natural product derivative library)242071340.164384ergosterol biosynthesis
0.0613.74E-6SGTC_1463k081-0032 34.1 μMChemDiv (Drug-like library)9777550.0789474

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1640st00907866.4 μM0.625690618TimTec (Natural product derivative library)301.340362.91115
SGTC_2784756767372.73 μM0.5576923471247Chembridge (Drug-like library)343.42011.29616iron homeostasis
SGTC_13963511-000798.6 μM0.484375X1396ChemDiv (Drug-like library)421.843265.08441mitochondrial processes
SGTC_1832st05411355 μM0.4603171251267TimTec (Natural product derivative library)364.39783.37116
SGTC_1962st07659564.5 μM0.444444279619TimTec (Natural product derivative library)242.316222.76513redox potentiating
SGTC_2300797669771.57 μM0.3454552236109Chembridge (Fragment library)238.756362.28912ERG2
SGTC_21695-(ethoxymethyl)quinolin-8-ol49.51 μM0.310345279621Chembridge (Fragment library)203.237121.92713
SGTC_29015-(ethoxymethyl)quinolin-8-ol39.67 μM0.310345279621Chembridge (Drug-like library)203.237121.92713
SGTC_2115tiliquinol12.77 μM0.30769271208Chembridge (Fragment library)159.184562.2612
SGTC_3283910933235.82 μM0.317027281Chembridge (Drug-like library)329.82393.45913DNA intercalators
SGTC_14394092-116846 μM0.2923082886240ChemDiv (Drug-like library)346.851123.8514