Lupinine o-methylbenzoicacid ester hydrochloride

2,3,4,6,7,8,9,9a-octahydro-1H-quinolizin-1-ylmethyl 2-methylbenzoate hydrochloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1639
Screen concentration 61.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 17037758
SMILES CC1=CC=CC=C1C(=O)OCC2CCCN3C2CCCC3.Cl
Standardized SMILES Cc1ccccc1C(=O)OCC2CCCN3CCCCC23
Molecular weight 323.8575
ALogP 4.19
H-bond donor count 1
H-bond acceptor count 3
Response signature ergosterol biosynthesis

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.51
% growth inhibition (Hom. pool) 4.72


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 17037758
Download HIP data (tab-delimited text)  (excel)
Gene:ARP2(YDL029W)|FD-Score:3.46|P-value:2.73E-4|Clearance:0.02||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC11(YJR076C)|FD-Score:4.64|P-value:1.70E-6|Clearance:1.14||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:ERG11(YHR007C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.44||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG7(YHR072W)|FD-Score:4.6|P-value:2.11E-6|Clearance:1.14||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GPI2(YPL076W)|FD-Score:-3.72|P-value:1.01E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:HEM12(YDR047W)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.03||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MAS2(YHR024C)|FD-Score:-3.32|P-value:4.42E-4|Clearance:0||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:SPC105(YGL093W)|FD-Score:3.36|P-value:3.94E-4|Clearance:0.06||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SRP54(YPR088C)|FD-Score:-3.25|P-value:5.84E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TAF9(YMR236W)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.06||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TFC8(YPL007C)|FD-Score:3.21|P-value:6.57E-4|Clearance:0.07||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YTH1(YPR107C)|FD-Score:5.76|P-value:4.28E-9|Clearance:1.14||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ARP2(YDL029W)|FD-Score:3.46|P-value:2.73E-4|Clearance:0.02||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:CDC11(YJR076C)|FD-Score:4.64|P-value:1.70E-6|Clearance:1.14||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:ERG11(YHR007C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.44||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:ERG7(YHR072W)|FD-Score:4.6|P-value:2.11E-6|Clearance:1.14||SGD DESC:Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis Gene:GPI2(YPL076W)|FD-Score:-3.72|P-value:1.01E-4|Clearance:0||SGD DESC:Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein Gene:HEM12(YDR047W)|FD-Score:3.38|P-value:3.58E-4|Clearance:0.03||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MAS2(YHR024C)|FD-Score:-3.32|P-value:4.42E-4|Clearance:0||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:SPC105(YGL093W)|FD-Score:3.36|P-value:3.94E-4|Clearance:0.06||SGD DESC:Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components Gene:SRP54(YPR088C)|FD-Score:-3.25|P-value:5.84E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TAF9(YMR236W)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.06||SGD DESC:Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3 Gene:TFC8(YPL007C)|FD-Score:3.21|P-value:6.57E-4|Clearance:0.07||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.3|P-value:4.91E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YTH1(YPR107C)|FD-Score:5.76|P-value:4.28E-9|Clearance:1.14||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 17037758
Download HOP data (tab-delimited text)  (excel)
Gene:BRE4(YDL231C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:BST1(YFL025C)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF120(YNL278W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COG1(YGL223C)|FD-Score:-3.9|P-value:4.78E-5||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG5(YNL051W)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-4.47|P-value:3.98E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR9(YOL145C)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DFG16(YOR030W)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DID4(YKL002W)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:ECI1(YLR284C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:EOS1(YNL080C)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:EPS1(YIL005W)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERP1(YAR002C-A)|FD-Score:4.38|P-value:6.03E-6||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FMP48(YGR052W_p)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GCV3(YAL044C)|FD-Score:3.89|P-value:4.96E-5||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GET2(YER083C)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HMG1(YML075C)|FD-Score:6.11|P-value:4.91E-10||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOT13(YKL084W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HSP82(YPL240C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HST1(YOL068C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HUR1(YGL168W)|FD-Score:-4.56|P-value:2.58E-6||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IRC10(YOL015W)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:NDL1(YLR254C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NOT5(YPR072W)|FD-Score:-3.16|P-value:7.82E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NYV1(YLR093C)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:OXR1(YPL196W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PAU23(YLR037C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEX15(YOL044W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PMT7(YDR307W_p)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PUG1(YER185W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RGP1(YDR137W)|FD-Score:-5.35|P-value:4.51E-8||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RGT1(YKL038W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RIC1(YLR039C)|FD-Score:-4.5|P-value:3.40E-6||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM9(YMR063W)|FD-Score:3.74|P-value:9.35E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROX1(YPR065W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL9A(YGL147C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:SSD1(YDR293C)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SVL3(YPL032C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SWD1(YAR003W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:THI21(YPL258C)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:THR1(YHR025W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:ULI1(YFR026C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:VPS45(YGL095C)|FD-Score:-4|P-value:3.19E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-4.64|P-value:1.75E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-3.85|P-value:5.93E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YER175W-A(YER175W-A_p)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YIL029C(YIL029C_p)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL100W(YIL100W_d)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YML034C-A(YML034C-A_d)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR018W(YMR018W_p)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR31(YFR049W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL057W(YNL057W_d)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL073C(YOL073C_p)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YOL085C(YOL085C_d)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YPL247C(YPL247C_p)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR077C(YPR077C_d)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPT10(YBR264C)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Gene:YPT6(YLR262C)|FD-Score:-5|P-value:2.80E-7||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:BRE4(YDL231C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport Gene:BST1(YFL025C)|FD-Score:-3.27|P-value:5.31E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:BTS1(YPL069C)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF120(YNL278W)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; CAF120 has a paralog, SKG3, that arose from the whole genome duplication Gene:CDC50(YCR094W)|FD-Score:3.79|P-value:7.60E-5||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COG1(YGL223C)|FD-Score:-3.9|P-value:4.78E-5||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG5(YNL051W)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG6(YNL041C)|FD-Score:-4.47|P-value:3.98E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR9(YOL145C)|FD-Score:-3.18|P-value:7.41E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:DFG16(YOR030W)|FD-Score:-3.32|P-value:4.56E-4||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DID4(YKL002W)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:ECI1(YLR284C)|FD-Score:3.12|P-value:8.91E-4||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:EOS1(YNL080C)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:EPS1(YIL005W)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:ER protein with chaperone and co-chaperone activity, involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family Gene:ERP1(YAR002C-A)|FD-Score:4.38|P-value:6.03E-6||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FMP48(YGR052W_p)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GCV3(YAL044C)|FD-Score:3.89|P-value:4.96E-5||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GET2(YER083C)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:HMG1(YML075C)|FD-Score:6.11|P-value:4.91E-10||SGD DESC:HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress Gene:HOT13(YKL084W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HSP82(YPL240C)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:HST1(YOL068C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:HUR1(YGL168W)|FD-Score:-4.56|P-value:2.58E-6||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IRC10(YOL015W)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:NDL1(YLR254C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:NOT5(YPR072W)|FD-Score:-3.16|P-value:7.82E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NYV1(YLR093C)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:OXR1(YPL196W)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PAU23(YLR037C)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PEX15(YOL044W)|FD-Score:3.51|P-value:2.20E-4||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PMT7(YDR307W_p)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:PUG1(YER185W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:RGP1(YDR137W)|FD-Score:-5.35|P-value:4.51E-8||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RGT1(YKL038W)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; RGT1 has a paralog, EDS1, that arose from the whole genome duplication Gene:RIC1(YLR039C)|FD-Score:-4.5|P-value:3.40E-6||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIM9(YMR063W)|FD-Score:3.74|P-value:9.35E-5||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROX1(YPR065W)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RPL9A(YGL147C)|FD-Score:3.2|P-value:6.99E-4||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:SSD1(YDR293C)|FD-Score:-3.38|P-value:3.61E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SVL3(YPL032C)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SWD1(YAR003W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:THI21(YPL258C)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p Gene:THR1(YHR025W)|FD-Score:-3.52|P-value:2.15E-4||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:ULI1(YFR026C)|FD-Score:-3.39|P-value:3.47E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:VPS45(YGL095C)|FD-Score:-4|P-value:3.19E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-4.64|P-value:1.75E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-3.85|P-value:5.93E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YER175W-A(YER175W-A_p)|FD-Score:3.1|P-value:9.74E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YIL029C(YIL029C_p)|FD-Score:3.39|P-value:3.44E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL100W(YIL100W_d)|FD-Score:-3.11|P-value:9.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YML034C-A(YML034C-A_d)|FD-Score:3.51|P-value:2.28E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR018W(YMR018W_p)|FD-Score:-3.79|P-value:7.49E-5||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR31(YFR049W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to human mitochondrial ribosomal protein MRP-S36 Gene:YNL057W(YNL057W_d)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL073C(YOL073C_p)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YOL085C(YOL085C_d)|FD-Score:3.25|P-value:5.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOL085W-A Gene:YPL247C(YPL247C_p)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest Gene:YPR077C(YPR077C_d)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPT10(YBR264C)|FD-Score:4.44|P-value:4.41E-6||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Gene:YPT6(YLR262C)|FD-Score:-5|P-value:2.80E-7||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR107C5.764.28E-91.14YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YJR076C4.641.70E-61.14CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YHR072W4.602.11E-61.14ERG7Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
YDL029W3.462.73E-40.02ARP2Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YMR236W3.442.92E-40.06TAF9Subunit (17 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
YDR047W3.383.58E-40.03HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YGL093W3.363.94E-40.06SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YMR290W-A_d3.304.91E-40.08YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YPL007C3.216.57E-40.07TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YHR007C3.158.28E-40.45ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YDR299W2.700.003450.13BFR2Essential protein that is a component of 90S preribosomes; may be involved in rRNA processing; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
YOR060C2.570.005130.06SLD7Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria
YOR254C2.500.006170.02SEC63Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
YDL207W2.490.006460.05GLE1Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
YMR113W2.430.007530.02FOL3Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML075C6.114.91E-10HMG1HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
YBR264C4.444.41E-6YPT10Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles
YAR002C-A4.386.03E-6ERP1Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication
YNL057W_d4.261.01E-5YNL057W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPL069C4.171.53E-5BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YPL258C4.161.59E-5THI21Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p
YLR254C4.121.86E-5NDL1Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends
YAL044C3.894.96E-5GCV3H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
YLR093C3.865.74E-5NYV1v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane
YCR094W3.797.60E-5CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YMR063W3.749.35E-5RIM9Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
YPR077C_d3.739.49E-5YPR077C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1
YPL240C3.681.18E-4HSP82Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication
YOL068C3.581.72E-4HST1NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance
YER185W3.581.75E-4PUG1Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins

GO enrichment analysis for SGTC_1639
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2421.57E-79SGTC_2648atorvastatin 78.7 μMMiscellaneous608230.108696azole & statin
0.2328.77E-73SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.241379ergosterol biosynthesis
0.2234.45E-67SGTC_2647atorvastatin 60.2 μMMiscellaneous608230.108696azole & statin
0.1943.28E-51SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.0985916ergosterol biosynthesis
0.1771.60E-42SGTC_21475545932 89.7 μMChembridge (Fragment library)7974040.206897ergosterol biosynthesis
0.1675.05E-38SGTC_21555570423 76.0 μMChembridge (Fragment library)7974020.16129ergosterol biosynthesis
0.1661.46E-37SGTC_2753fluvastatin 17.1 μMMiscellaneous4461550.117647azole & statin
0.1641.34E-36SGTC_21856113692 200.0 μMChembridge (Fragment library)8741500.174603ergosterol biosynthesis
0.1611.93E-35SGTC_1752st044819 83.0 μMTimTec (Natural product derivative library)4412090.109375
0.1602.99E-35SGTC_31009120536 49.5 μMChembridge (Drug-like library)164211960.0617284
0.1511.20E-31SGTC_8650488-0499 301.0 μMChemDiv (Drug-like library)96346510.0735294
0.1423.63E-28SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.214286fatty acid desaturase (OLE1)
0.1424.07E-28SGTC_1638st008370 60.6 μMTimTec (Natural product derivative library)242071340.75ergosterol biosynthesis
0.1411.12E-27SGTC_14354092-0789 56.7 μMChemDiv (Drug-like library)22482850.164179plasma membrane duress
0.1404.61E-27SGTC_830170-0301 39.0 μMChemDiv (Drug-like library)30897160.0634921endomembrane recycling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1660st01296028.1 μM124207266TimTec (Natural product derivative library)580.4471550.052815iron homeostasis
SGTC_1638st00837060.6 μM0.7524207134TimTec (Natural product derivative library)325.830363.46524ergosterol biosynthesis
SGTC_1797st04869556.8 μM0.592593592262TimTec (Natural product derivative library)352.266084.07403NEO1
SGTC_1629st00508544.9 μM0.52727324207091TimTec (Natural product derivative library)445.334994.49704RPP1 & pyrimidine depletion
SGTC_1991st0730008.92 μM0.4925374301969TimTec (Natural product derivative library)466.57415.92105NEO1
SGTC_1969st06947940.1 μM0.4050633895485TimTec (Natural product derivative library)498.948895.96209
SGTC_1657st01293943.4 μM0.38297924207257TimTec (Natural product derivative library)462.314515-2.478914
SGTC_3206911435049.47 μM0.32203417087243Chembridge (Drug-like library)283.321783.53513
SGTC_22957971645200 μM0.3148152978226Chembridge (Fragment library)204.268242.09911
SGTC_3045909295049.47 μM0.29687544120432Chembridge (Drug-like library)365.296784.31712