st009582

2-hydroxy-5-[(6-oxocyclohexa-2,4-dien-1-ylidene)methylamino]benzoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1642
Screen concentration 77.7 μM
Source TimTec (Natural product derivative library)
PubChem CID 6739311
SMILES C1=CC(=CNC2=CC(=C(C=C2)O)C(=O)O)C(=O)C=C1
Standardized SMILES OC(=O)c1cc(ccc1O)N=Cc2ccccc2O
Molecular weight 257.2414
ALogP 1.49
H-bond donor count 3
H-bond acceptor count 5
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.29
% growth inhibition (Hom. pool) 4.72


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6739311
Download HIP data (tab-delimited text)  (excel)
Gene:ALG1(YBR110W)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.03||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:BET1(YIL004C)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.03||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:BRL1(YHR036W)|FD-Score:-3.37|P-value:3.83E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:BRX1(YOL077C)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.27||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:FOL3(YMR113W)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.07||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:HAS1(YMR290C)|FD-Score:3.76|P-value:8.66E-5|Clearance:0.16||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:MES1(YGR264C)|FD-Score:-3.24|P-value:5.95E-4|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:NOP53(YPL146C)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.01||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PRP28(YDR243C)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.11||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:RET1(YOR207C)|FD-Score:5.53|P-value:1.62E-8|Clearance:1.16||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RLP7(YNL002C)|FD-Score:3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPB8(YOR224C)|FD-Score:4.37|P-value:6.20E-6|Clearance:0.18||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC37(YKR025W)|FD-Score:3.1|P-value:9.57E-4|Clearance:0.05||SGD DESC:RNA polymerase III subunit C37 Gene:RPO31(YOR116C)|FD-Score:8.86|P-value:3.87E-19|Clearance:3.34||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:10.1|P-value:2.18E-24|Clearance:3.34||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP46(YGR095C)|FD-Score:3.21|P-value:6.59E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SEC31(YDL195W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.06||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SWD2(YKL018W)|FD-Score:4.1|P-value:2.11E-5|Clearance:0.34||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TAF8(YML114C)|FD-Score:-3.33|P-value:4.29E-4|Clearance:0||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:ALG1(YBR110W)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.03||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:BET1(YIL004C)|FD-Score:4.19|P-value:1.41E-5|Clearance:0.03||SGD DESC:Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins Gene:BRL1(YHR036W)|FD-Score:-3.37|P-value:3.83E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export Gene:BRX1(YOL077C)|FD-Score:3.53|P-value:2.04E-4|Clearance:0.27||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:FOL3(YMR113W)|FD-Score:4.16|P-value:1.58E-5|Clearance:0.07||SGD DESC:Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis Gene:HAS1(YMR290C)|FD-Score:3.76|P-value:8.66E-5|Clearance:0.16||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:MES1(YGR264C)|FD-Score:-3.24|P-value:5.95E-4|Clearance:0||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:NOP53(YPL146C)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.01||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:PRP28(YDR243C)|FD-Score:3.21|P-value:6.64E-4|Clearance:0.11||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:RET1(YOR207C)|FD-Score:5.53|P-value:1.62E-8|Clearance:1.16||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RLP7(YNL002C)|FD-Score:3.21|P-value:6.62E-4|Clearance:0||SGD DESC:Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs Gene:RPB8(YOR224C)|FD-Score:4.37|P-value:6.20E-6|Clearance:0.18||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC37(YKR025W)|FD-Score:3.1|P-value:9.57E-4|Clearance:0.05||SGD DESC:RNA polymerase III subunit C37 Gene:RPO31(YOR116C)|FD-Score:8.86|P-value:3.87E-19|Clearance:3.34||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RPP1(YHR062C)|FD-Score:10.1|P-value:2.18E-24|Clearance:3.34||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP46(YGR095C)|FD-Score:3.21|P-value:6.59E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SEC31(YDL195W)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.06||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SWD2(YKL018W)|FD-Score:4.1|P-value:2.11E-5|Clearance:0.34||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination Gene:TAF8(YML114C)|FD-Score:-3.33|P-value:4.29E-4|Clearance:0||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6739311
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADO1(YJR105W)|FD-Score:-4.1|P-value:2.08E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AGC1(YPR021C)|FD-Score:6.47|P-value:4.93E-11||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:AIM44(YPL158C)|FD-Score:6.3|P-value:1.47E-10||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ALD6(YPL061W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ARD1(YHR013C)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ASM4(YDL088C)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:AVL9(YLR114C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BIM1(YER016W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BIO2(YGR286C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:COG6(YNL041C)|FD-Score:3.8|P-value:7.09E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTL1(YMR180C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:FRT2(YAL028W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:FSF1(YOR271C_p)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:GEP4(YHR100C)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GET2(YER083C)|FD-Score:5.49|P-value:2.04E-8||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GUP1(YGL084C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HEH2(YDR458C)|FD-Score:-4.08|P-value:2.27E-5||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HER2(YMR293C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HMO1(YDR174W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HSL7(YBR133C)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:KRE6(YPR159W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:KTR2(YKR061W)|FD-Score:3.85|P-value:5.90E-5||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LCL3(YGL085W_p)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:LRS4(YDR439W)|FD-Score:4.39|P-value:5.63E-6||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MLP2(YIL149C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MRP51(YPL118W)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRS4(YKR052C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MVB12(YGR206W)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NAP1(YKR048C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NTE1(YML059C)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:NUP170(YBL079W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PDC1(YLR044C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PHA2(YNL316C)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PHO4(YFR034C)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PPH3(YDR075W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:REE1(YJL217W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle Gene:RIM9(YMR063W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL31A(YDL075W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RSC1(YGR056W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SGO1(YOR073W)|FD-Score:-3.85|P-value:5.99E-5||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHE10(YGL228W)|FD-Score:-3.77|P-value:8.27E-5||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SSB1(YDL229W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STR3(YGL184C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p Gene:SYN8(YAL014C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:UBP7(YIL156W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:VPS1(YKR001C)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS55(YJR044C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:WSC2(YNL283C)|FD-Score:4.94|P-value:3.86E-7||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBL071C(YBL071C_d)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YCR015C(YCR015C_p)|FD-Score:4.51|P-value:3.29E-6||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function Gene:YGR259C(YGR259C_d)|FD-Score:7.95|P-value:9.27E-16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YLL007C(YLL007C_p)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR366W(YLR366W_d)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL193W(YNL193W_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YNL205C(YNL205C_d)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL047C(YOL047C_p)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR082C(YOR082C_d)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YPR146C(YPR146C_d)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACE2(YLR131C)|FD-Score:3.43|P-value:3.00E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ADO1(YJR105W)|FD-Score:-4.1|P-value:2.08E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AGC1(YPR021C)|FD-Score:6.47|P-value:4.93E-11||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:AIM44(YPL158C)|FD-Score:6.3|P-value:1.47E-10||SGD DESC:Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ALD6(YPL061W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ARD1(YHR013C)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ASM4(YDL088C)|FD-Score:4.74|P-value:1.09E-6||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:AVL9(YLR114C)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BIM1(YER016W)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BIO2(YGR286C)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:COG6(YNL041C)|FD-Score:3.8|P-value:7.09E-5||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTL1(YMR180C)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm Gene:FRT2(YAL028W)|FD-Score:3.19|P-value:7.19E-4||SGD DESC:Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication Gene:FSF1(YOR271C_p)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:GEP4(YHR100C)|FD-Score:-3.63|P-value:1.42E-4||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GET2(YER083C)|FD-Score:5.49|P-value:2.04E-8||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GUP1(YGL084C)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:HEH2(YDR458C)|FD-Score:-4.08|P-value:2.27E-5||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HER2(YMR293C)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase Gene:HMO1(YDR174W)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase Gene:HSL7(YBR133C)|FD-Score:3.11|P-value:9.45E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:KRE6(YPR159W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:KTR2(YKR061W)|FD-Score:3.85|P-value:5.90E-5||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LCL3(YGL085W_p)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS Gene:LRS4(YDR439W)|FD-Score:4.39|P-value:5.63E-6||SGD DESC:Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:MLP2(YIL149C)|FD-Score:3.25|P-value:5.69E-4||SGD DESC:Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length Gene:MRP51(YPL118W)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRS4(YKR052C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MVB12(YGR206W)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin Gene:NAP1(YKR048C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NTE1(YML059C)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:NUP170(YBL079W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PDC1(YLR044C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PHA2(YNL316C)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PHO4(YFR034C)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PPH3(YDR075W)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes Gene:REE1(YJL217W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle Gene:RIM9(YMR063W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL31A(YDL075W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RSC1(YGR056W)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SGO1(YOR073W)|FD-Score:-3.85|P-value:5.99E-5||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SHE10(YGL228W)|FD-Score:-3.77|P-value:8.27E-5||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SSB1(YDL229W)|FD-Score:3.18|P-value:7.42E-4||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:SSD1(YDR293C)|FD-Score:-3.29|P-value:5.07E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:STR3(YGL184C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Peroxisomal cystathionine beta-lyase, converts cystathionine into homocysteine; may be redox regulated by Gto1p Gene:SYN8(YAL014C)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:UBP7(YIL156W)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions Gene:VPS1(YKR001C)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS55(YJR044C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:WSC2(YNL283C)|FD-Score:4.94|P-value:3.86E-7||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBL071C(YBL071C_d)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Dubious open reading frame, predicted protein contains a peroxisomal targeting signal Gene:YCR015C(YCR015C_p)|FD-Score:4.51|P-value:3.29E-6||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Putative protein of unknown function Gene:YGR259C(YGR259C_d)|FD-Score:7.95|P-value:9.27E-16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YLL007C(YLL007C_p)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene Gene:YLR366W(YLR366W_d)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL193W(YNL193W_p)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YNL205C(YNL205C_d)|FD-Score:4.05|P-value:2.51E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL047C(YOL047C_p)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR082C(YOR082C_d)|FD-Score:3.16|P-value:7.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene YOR083W Gene:YPR146C(YPR146C_d)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C10.102.18E-243.34RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR116C8.863.87E-193.34RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YOR207C5.531.62E-81.16RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YOR224C4.376.20E-60.18RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YIL004C4.191.41E-50.03BET1Type II membrane protein required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins
YMR113W4.161.58E-50.07FOL3Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
YKL018W4.102.11E-50.34SWD2Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination
YMR290C3.768.66E-50.16HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
YDL195W3.591.65E-40.06SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YOL077C3.532.04E-40.28BRX1Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
YBR110W3.265.58E-40.03ALG1Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
YPL146C3.236.29E-40.01NOP53Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired
YGR095C3.216.59E-40.00RRP46Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
YNL002C3.216.62E-49.36E-4RLP7Nucleolar protein with similarity to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs
YDR243C3.216.64E-40.11PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR259C_d7.959.27E-16YGR259C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W
YPR021C6.474.93E-11AGC1Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis
YPL158C6.301.47E-10AIM44Protein of unknown function; GFP-fusion protein localizes to the bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress
YER083C5.492.04E-8GET2Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
YNL283C4.943.86E-7WSC2Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response
YDL088C4.741.09E-6ASM4FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication
YMR063W4.701.29E-6RIM9Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
YCR015C_p4.513.29E-6YCR015C_pPutative protein of unknown function; YCR015C is not an essential gene
YGR206W4.415.14E-6MVB12ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
YDR439W4.395.63E-6LRS4Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
YAL014C4.211.28E-5SYN8Endosomal SNARE related to mammalian syntaxin 8
YBL071C_d4.102.07E-5YBL071C_dDubious open reading frame, predicted protein contains a peroxisomal targeting signal
YDR174W4.092.20E-5HMO1Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase
YNL205C_d4.052.51E-5YNL205C_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YML059C3.963.79E-5NTE1Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes

GO enrichment analysis for SGTC_1642
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2161.71E-63SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.078125RNA pol III & RNase P/MRP
0.2101.20E-59SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.106195RNA pol III & RNase P/MRP
0.1831.02E-45SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.116667RNA pol III & RNase P/MRP
0.1713.79E-40SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465410.108108RNA pol III & RNase P/MRP
0.1675.65E-38SGTC_2522biochanin a 4.9 μMMicrosource (Natural product library)52803730.125RNA pol III & RNase P/MRP
0.1631.46E-36SGTC_23067482397 179.5 μMChembridge (Fragment library)8914480.218182RPP1 & pyrimidine depletion
0.1625.61E-36SGTC_1915st067105 30.6 μMTimTec (Natural product derivative library)697510.125RNA pol III & RNase P/MRP
0.1561.82E-33SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.123077
0.1511.82E-31SGTC_389tpck 16.7 μMMiscellaneous4396470.123077
0.1504.14E-31SGTC_15258-methoxypsoralen 92.5 μMTimTec (Pure natural product library)41140.078125DNA damage response
0.1505.91E-31SGTC_15346-azauridine 81.6 μMTimTec (Pure natural product library)2335020.0606061RNA pol III & RNase P/MRP
0.1411.11E-27SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.360656
0.1396.23E-27SGTC_8282074-0507 139.0 μMChemDiv (Drug-like library)X8280.268657RPP1 & pyrimidine depletion
0.1373.09E-26SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.108108RPP1 & pyrimidine depletion
0.1331.38E-24SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0533333RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7851270-011165.3 μM0.6222226741994ChemDiv (Drug-like library)307.300122.53735
SGTC_8670518-0118155 μM0.5111116848158ChemDiv (Drug-like library)256.256681.40235DNA intercalators
SGTC_2235salicylic acid500 μM0.472222338Miscellaneous138.120741.21723
SGTC_12700831-117017.3 μM0.450986764716ChemDiv (Drug-like library)380.234664.25113
SGTC_8460335-0850148 μM0.4285716819598ChemDiv (Drug-like library)331.45074.50912ubiquinone biosynthesis & proteasome
SGTC_18233-hydroxy-2-naphthoic acid106 μM0.4257104TimTec (Natural product derivative library)188.179422.12623
SGTC_30833-058795.58 μM0.4130435382644ChemDiv (Drug-like library)240.257281.00623redox potentiating
SGTC_8690564-008044.1 μM0.4130436843321ChemDiv (Drug-like library)317.34133.93125
SGTC_2768sulfasalazine50.2 μM0.4035095384001Miscellaneous398.392562.01339
SGTC_6671498-104459 μM0.3921576739982ChemDiv (Drug-like library)291.300722.77924RPP1 & pyrimidine depletion