st011737

(4E)-N-(furan-2-ylmethyl)-2,2,5,9-tetramethyldeca-4,8-dien-1-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1649
Screen concentration 51.3 μM
Source TimTec (Natural product derivative library)
PubChem CID 1761035
SMILES CC(=CCCC(=CCC(C)(C)CNCC1=CC=CO1)C)C
Standardized SMILES CC(=CCCC(=CCC(C)(C)CNCc1occc1)C)C
Molecular weight 289.4555
ALogP 5.21
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.29
% growth inhibition (Hom. pool) 9.01


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1761035
Download HIP data (tab-delimited text)  (excel)
Gene:GCD11(YER025W)|FD-Score:-3.48|P-value:2.51E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:HSH49(YOR319W)|FD-Score:-4.65|P-value:1.69E-6|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MPE1(YKL059C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.33||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NIC96(YFR002W)|FD-Score:-3.9|P-value:4.88E-5|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOP2(YNL061W)|FD-Score:-3.92|P-value:4.46E-5|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP56(YLR197W)|FD-Score:-4.04|P-value:2.69E-5|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:OLE1(YGL055W)|FD-Score:7.43|P-value:5.61E-14|Clearance:3.65||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:RPB7(YDR404C)|FD-Score:-3.56|P-value:1.82E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPF1(YHR088W)|FD-Score:3.77|P-value:8.03E-5|Clearance:0.13||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RRP42(YDL111C)|FD-Score:-3.55|P-value:1.92E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SEN34(YAR008W)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.27||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:UTP14(YML093W)|FD-Score:-3.44|P-value:2.86E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTR5(YEL035C_p)|FD-Score:-3.16|P-value:7.97E-4|Clearance:0||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YDL152W(YDL152W_d)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YLR198C(YLR198C_d)|FD-Score:-3.51|P-value:2.21E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:GCD11(YER025W)|FD-Score:-3.48|P-value:2.51E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:HSH49(YOR319W)|FD-Score:-4.65|P-value:1.69E-6|Clearance:0||SGD DESC:U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM) Gene:MPE1(YKL059C)|FD-Score:3.65|P-value:1.32E-4|Clearance:0.33||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NIC96(YFR002W)|FD-Score:-3.9|P-value:4.88E-5|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p) Gene:NOP2(YNL061W)|FD-Score:-3.92|P-value:4.46E-5|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP56(YLR197W)|FD-Score:-4.04|P-value:2.69E-5|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:OLE1(YGL055W)|FD-Score:7.43|P-value:5.61E-14|Clearance:3.65||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:RPB7(YDR404C)|FD-Score:-3.56|P-value:1.82E-4|Clearance:0||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPF1(YHR088W)|FD-Score:3.77|P-value:8.03E-5|Clearance:0.13||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:RRP42(YDL111C)|FD-Score:-3.55|P-value:1.92E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7) Gene:SEN34(YAR008W)|FD-Score:3.32|P-value:4.47E-4|Clearance:0.27||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease Gene:UTP14(YML093W)|FD-Score:-3.44|P-value:2.86E-4|Clearance:0||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:UTR5(YEL035C_p)|FD-Score:-3.16|P-value:7.97E-4|Clearance:0||SGD DESC:Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions Gene:YDL152W(YDL152W_d)|FD-Score:-3.47|P-value:2.58E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YLR198C(YLR198C_d)|FD-Score:-3.51|P-value:2.21E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1761035
Download HOP data (tab-delimited text)  (excel)
Gene:AHA1(YDR214W)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:ALY1(YKR021W)|FD-Score:5.64|P-value:8.30E-9||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:AMN1(YBR158W)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:ARO1(YDR127W)|FD-Score:12.1|P-value:4.41E-34||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:4.77|P-value:9.37E-7||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:7.87|P-value:1.80E-15||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG8(YBL078C)|FD-Score:6.5|P-value:4.05E-11||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BCK1(YJL095W)|FD-Score:5.77|P-value:3.97E-9||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUD25(YER014C-A)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CDC50(YCR094W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSE4(YNR067C)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:FEN2(YCR028C)|FD-Score:-4.44|P-value:4.46E-6||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FMO1(YHR176W)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GAL7(YBR018C)|FD-Score:3.72|P-value:9.91E-5||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GCN2(YDR283C)|FD-Score:8.78|P-value:8.16E-19||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:7.48|P-value:3.61E-14||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:10.2|P-value:1.10E-24||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:8.72|P-value:1.43E-18||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GRX6(YDL010W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HEH2(YDR458C)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:IRS4(YKR019C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LOH1(YJL038C)|FD-Score:3.8|P-value:7.28E-5||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MRI1(YPR118W)|FD-Score:4.86|P-value:5.84E-7||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MSD1(YPL104W)|FD-Score:3.09|P-value:0.00100||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:OPT2(YPR194C)|FD-Score:4.57|P-value:2.45E-6||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:OSH3(YHR073W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEX13(YLR191W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PHM6(YDR281C)|FD-Score:-4.08|P-value:2.29E-5||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:RGP1(YDR137W)|FD-Score:-4.21|P-value:1.29E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:SCW11(YGL028C)|FD-Score:-3.85|P-value:5.95E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SIL1(YOL031C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SKP2(YNL311C)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SLT2(YHR030C)|FD-Score:9.07|P-value:6.20E-20||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT2(YER161C)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSA2(YLL024C)|FD-Score:3.79|P-value:7.54E-5||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:SWA2(YDR320C)|FD-Score:-7.98|P-value:7.51E-16||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TMA108(YIL137C)|FD-Score:4.61|P-value:2.01E-6||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TPN1(YGL186C)|FD-Score:-4|P-value:3.10E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:9.26|P-value:1.03E-20||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.97|P-value:1.63E-12||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:11.5|P-value:7.09E-31||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:9.59|P-value:4.50E-22||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:7.87|P-value:1.81E-15||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UGA4(YDL210W)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane Gene:VPS45(YGL095C)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-5.24|P-value:7.98E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL055C(YBL055C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBL095W(YBL095W_p)|FD-Score:4.42|P-value:4.98E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR178W(YBR178W_d)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCR061W(YCR061W_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR008C(YDR008C_d)|FD-Score:8.91|P-value:2.60E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:4.68|P-value:1.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL012W(YFL012W_p)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL117W(YGL117W_p)|FD-Score:4.83|P-value:6.70E-7||SGD DESC:Putative protein of unknown function Gene:YLR345W(YLR345W_p)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YNL146W(YNL146W_p)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPR077C(YPR077C_d)|FD-Score:4.45|P-value:4.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPT6(YLR262C)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:AHA1(YDR214W)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:ALY1(YKR021W)|FD-Score:5.64|P-value:8.30E-9||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:AMN1(YBR158W)|FD-Score:3.77|P-value:8.06E-5||SGD DESC:Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN) Gene:ARO1(YDR127W)|FD-Score:12.1|P-value:4.41E-34||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:4.77|P-value:9.37E-7||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:7.87|P-value:1.80E-15||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG8(YBL078C)|FD-Score:6.5|P-value:4.05E-11||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BCK1(YJL095W)|FD-Score:5.77|P-value:3.97E-9||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUD25(YER014C-A)|FD-Score:-3.24|P-value:5.90E-4||SGD DESC:Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern Gene:CDC50(YCR094W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DSE4(YNR067C)|FD-Score:4.49|P-value:3.54E-6||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother Gene:FEN2(YCR028C)|FD-Score:-4.44|P-value:4.46E-6||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FMO1(YHR176W)|FD-Score:4.19|P-value:1.38E-5||SGD DESC:Flavin-containing monooxygenase, localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins Gene:GAL7(YBR018C)|FD-Score:3.72|P-value:9.91E-5||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GCN2(YDR283C)|FD-Score:8.78|P-value:8.16E-19||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:7.48|P-value:3.61E-14||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:10.2|P-value:1.10E-24||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:8.72|P-value:1.43E-18||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GRX6(YDL010W)|FD-Score:3.93|P-value:4.22E-5||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HEH2(YDR458C)|FD-Score:-3.34|P-value:4.26E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:IRS4(YKR019C)|FD-Score:-3.66|P-value:1.26E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LEM3(YNL323W)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LOH1(YJL038C)|FD-Score:3.8|P-value:7.28E-5||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MRI1(YPR118W)|FD-Score:4.86|P-value:5.84E-7||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MSD1(YPL104W)|FD-Score:3.09|P-value:0.00100||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:OPT2(YPR194C)|FD-Score:4.57|P-value:2.45E-6||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:OSH3(YHR073W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PEX13(YLR191W)|FD-Score:3.62|P-value:1.47E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PHM6(YDR281C)|FD-Score:-4.08|P-value:2.29E-5||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:RGP1(YDR137W)|FD-Score:-4.21|P-value:1.29E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:SCW11(YGL028C)|FD-Score:-3.85|P-value:5.95E-5||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SIL1(YOL031C)|FD-Score:3.17|P-value:7.56E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SKP2(YNL311C)|FD-Score:3.85|P-value:5.80E-5||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SLT2(YHR030C)|FD-Score:9.07|P-value:6.20E-20||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPT2(YER161C)|FD-Score:3.26|P-value:5.67E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSA2(YLL024C)|FD-Score:3.79|P-value:7.54E-5||SGD DESC:ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall; 98% identical with paralog Ssa1p, but subtle differences between the two proteins provide functional specificity with respect to propagation of yeast [URE3] prions and vacuolar-mediated degradations of gluconeogenesis enzymes Gene:SWA2(YDR320C)|FD-Score:-7.98|P-value:7.51E-16||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TMA108(YIL137C)|FD-Score:4.61|P-value:2.01E-6||SGD DESC:Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease Gene:TPN1(YGL186C)|FD-Score:-4|P-value:3.10E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:9.26|P-value:1.03E-20||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:6.97|P-value:1.63E-12||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:11.5|P-value:7.09E-31||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:9.59|P-value:4.50E-22||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:7.87|P-value:1.81E-15||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UGA4(YDL210W)|FD-Score:-3.21|P-value:6.54E-4||SGD DESC:Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane Gene:VPS45(YGL095C)|FD-Score:-3.98|P-value:3.39E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS61(YDR136C_d)|FD-Score:-5.24|P-value:7.98E-8||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-3.31|P-value:4.70E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YBL055C(YBL055C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YBL095W(YBL095W_p)|FD-Score:4.42|P-value:4.98E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YBR178W(YBR178W_d)|FD-Score:-3.23|P-value:6.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YCR061W(YCR061W_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDR008C(YDR008C_d)|FD-Score:8.91|P-value:2.60E-19||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR433W(YDR433W_d)|FD-Score:4.68|P-value:1.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL012W(YFL012W_p)|FD-Score:3.76|P-value:8.39E-5||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL117W(YGL117W_p)|FD-Score:4.83|P-value:6.70E-7||SGD DESC:Putative protein of unknown function Gene:YLR345W(YLR345W_p)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YNL146W(YNL146W_p)|FD-Score:4.15|P-value:1.68E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YPR077C(YPR077C_d)|FD-Score:4.45|P-value:4.23E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPT6(YLR262C)|FD-Score:-3.98|P-value:3.48E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL055W7.435.61E-143.65OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YHR088W3.778.03E-50.13RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YKL059C3.651.32E-40.33MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YAR008W3.324.47E-40.27SEN34Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
YBL004W3.050.001140.12UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YML064C2.930.001720.01TEM1GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis
YGL150C2.910.001800.10INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YJL002C2.810.002460.00OST1Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YNL189W2.810.002500.03SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YKR063C2.770.002780.08LAS1Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability
YIR015W2.690.003590.02RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YLR323C2.670.003780.04CWC24Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
YHR188C2.630.004310.07GPI16Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
YDL207W2.560.005250.02GLE1Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export
YFL029C2.540.005560.07CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR127W12.104.41E-34ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YKL211C11.507.09E-31TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YKR026C10.201.10E-24GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YDR354W9.594.50E-22TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR007W9.261.03E-20TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YHR030C9.076.20E-20SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
YDR008C_d8.912.60E-19YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR283C8.788.16E-19GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YEL009C8.721.43E-18GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR035W7.871.80E-15ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YGL026C7.871.81E-15TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YFR009W7.483.61E-14GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YER090W6.971.63E-12TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YBL078C6.504.05E-11ATG8Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
YJL095W5.773.97E-9BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p

GO enrichment analysis for SGTC_1649
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.6020SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.038961
0.5840SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0697674
0.5630SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0421053
0.5480SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.038961
0.5310SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.0597015plasma membrane duress
0.5180SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0869565
0.5120SGTC_18825615643 20.0 μMMiscellaneous22530750.0823529TRP & mitochondrial translation
0.5110SGTC_14274048-4900 550.0 nMChemDiv (Drug-like library)241325860.0746269
0.4990SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0694444
0.4920SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.0704225
0.4920SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.025641
0.4870SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.043956
0.4840SGTC_33089128332 17.6 μMChembridge (Drug-like library)173328050.148649plasma membrane duress
0.4800SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.097561
0.4760SGTC_5423381-0244 89.3 μMChemDiv (Drug-like library)11128430.0897436

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2624nerol100 μM0.319149637566Microsource (Natural product library)154.249322.93411
SGTC_1588farnesol29.9 μM0.3125445070TimTec (Pure natural product library)222.366344.7611
SGTC_2606farnesol9.78 μM0.3125445070Microsource (Natural product library)222.366344.7611
SGTC_2944906734625.62 μM0.3050856473075Chembridge (Drug-like library)324.20184.1723
SGTC_9322857-0803154 μM0.298246674898ChemDiv (Drug-like library)280.11732.55112
SGTC_22737815444169.05 μM0.2950822824616Chembridge (Fragment library)228.268182.00424
SGTC_23327982454138.89 μM0.2857142981748Chembridge (Fragment library)247.316123.15223
SGTC_2849901932112.99 μM0.2812516489627Chembridge (Drug-like library)275.365983.21713
SGTC_22717849410118.62 μM0.278689972430Chembridge (Fragment library)233.289542.66623
SGTC_2499avocadenofuran51.48 μM0.2777786857792Microsource (Natural product library)248.403626.37301
SGTC_9463434-133487.4 μM0.2656254047976ChemDiv (Drug-like library)266.33932.6324