st012112

1-(3,4-dihydro-1H-isoquinolin-2-yl)-2-(2-methoxyphenyl)ethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1653
Screen concentration 65.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 720429
SMILES COC1=CC=CC=C1CC(=O)N2CCC3=CC=CC=C3C2
Standardized SMILES COc1ccccc1CC(=O)N2CCc3ccccc3C2
Molecular weight 281.349
ALogP 2.92
H-bond donor count 0
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.85
% growth inhibition (Hom. pool) 3.48


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 720429
Download HIP data (tab-delimited text)  (excel)
Gene:CDC11(YJR076C)|FD-Score:3.83|P-value:6.52E-5|Clearance:0.31||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:ERG27(YLR100W)|FD-Score:-3.42|P-value:3.18E-4|Clearance:0||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:KAE1(YKR038C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.07||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:MCM4(YPR019W)|FD-Score:3.19|P-value:7.22E-4|Clearance:0.01||SGD DESC:Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p Gene:MEC1(YBR136W)|FD-Score:6.06|P-value:6.82E-10|Clearance:1.62||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:RPP1(YHR062C)|FD-Score:5.45|P-value:2.55E-8|Clearance:1.62||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT5(YOR117W)|FD-Score:-3.24|P-value:6.01E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SDS22(YKL193C)|FD-Score:3.32|P-value:4.54E-4|Clearance:0.06||SGD DESC:Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function Gene:SEC13(YLR208W)|FD-Score:3.18|P-value:7.37E-4|Clearance:0.32||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SPT15(YER148W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.2||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability Gene:CDC11(YJR076C)|FD-Score:3.83|P-value:6.52E-5|Clearance:0.31||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:ERG27(YLR100W)|FD-Score:-3.42|P-value:3.18E-4|Clearance:0||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:KAE1(YKR038C)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.07||SGD DESC:Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription Gene:MCM4(YPR019W)|FD-Score:3.19|P-value:7.22E-4|Clearance:0.01||SGD DESC:Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p Gene:MEC1(YBR136W)|FD-Score:6.06|P-value:6.82E-10|Clearance:1.62||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:RPP1(YHR062C)|FD-Score:5.45|P-value:2.55E-8|Clearance:1.62||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT5(YOR117W)|FD-Score:-3.24|P-value:6.01E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:SDS22(YKL193C)|FD-Score:3.32|P-value:4.54E-4|Clearance:0.06||SGD DESC:Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function Gene:SEC13(YLR208W)|FD-Score:3.18|P-value:7.37E-4|Clearance:0.32||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SPT15(YER148W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.2||SGD DESC:TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 720429
Download HOP data (tab-delimited text)  (excel)
Gene:ADE4(YMR300C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:BDS1(YOL164W)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BEM4(YPL161C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:FEN2(YCR028C)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FRA2(YGL220W)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:FUI1(YBL042C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:ICL2(YPR006C)|FD-Score:5.91|P-value:1.75E-9||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:IME4(YGL192W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:INO2(YDR123C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:JEN1(YKL217W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:LIP5(YOR196C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MCT1(YOR221C)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDV1(YJL112W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:NCA2(YPR155C)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p Gene:NCS6(YGL211W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:PET20(YPL159C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PET54(YGR222W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PHA2(YNL316C)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PMP3(YDR276C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PMS1(YNL082W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PUS2(YGL063W)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RAD51(YER095W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD57(YDR004W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RBG2(YGR173W)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RNH70(YGR276C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:SAK1(YER129W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SCD6(YPR129W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SNL1(YIL016W)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SNO2(YNL334C)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SSO2(YMR183C)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:SWA2(YDR320C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TRP2(YER090W)|FD-Score:5.7|P-value:6.12E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.37|P-value:6.25E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUB3(YML124C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:VAC7(YNL054W)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS25(YJR102C)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YAP1(YML007W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR138C(YBR138C_p)|FD-Score:4|P-value:3.18E-5||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.49|P-value:3.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YGR064W(YGR064W_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YKE2(YLR200W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YMR010W(YMR010W_p)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR158W-B(YMR158W-B_d)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YNR071C(YNR071C_p)|FD-Score:4.35|P-value:6.91E-6||SGD DESC:Putative aldose 1-epimerase Gene:YOL046C(YOL046C_d)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YPL162C(YPL162C_p)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPL216W(YPL216W_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPL225W(YPL225W)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPT31(YER031C)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:ADE4(YMR300C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:BDS1(YOL164W)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Bacterially-derived sulfatase required for use of alkyl- and aryl-sulfates as sulfur sources Gene:BEM4(YPL161C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:FEN2(YCR028C)|FD-Score:-3.68|P-value:1.19E-4||SGD DESC:Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:FRA2(YGL220W)|FD-Score:3.87|P-value:5.40E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:FUI1(YBL042C)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:ICL2(YPR006C)|FD-Score:5.91|P-value:1.75E-9||SGD DESC:2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol Gene:IME4(YGL192W)|FD-Score:3.33|P-value:4.40E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:INO2(YDR123C)|FD-Score:4.1|P-value:2.05E-5||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:JEN1(YKL217W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:LIP5(YOR196C)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MCT1(YOR221C)|FD-Score:4.58|P-value:2.32E-6||SGD DESC:Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling Gene:MDV1(YJL112W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication Gene:NCA2(YPR155C)|FD-Score:-3.27|P-value:5.44E-4||SGD DESC:Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p Gene:NCS6(YGL211W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:PET20(YPL159C)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PET54(YGR222W)|FD-Score:3.23|P-value:6.10E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PHA2(YNL316C)|FD-Score:3.77|P-value:8.08E-5||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PMP3(YDR276C)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential Gene:PMS1(YNL082W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PUS2(YGL063W)|FD-Score:-4|P-value:3.17E-5||SGD DESC:Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification Gene:RAD51(YER095W)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RAD57(YDR004W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RBG2(YGR173W)|FD-Score:3.93|P-value:4.33E-5||SGD DESC:Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein Gene:RNH70(YGR276C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:SAK1(YER129W)|FD-Score:3.76|P-value:8.53E-5||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SCD6(YPR129W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SNL1(YIL016W)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Ribosome-associated protein proposed to act in protein synthesis and nuclear pore complex biogenesis and maintenance as well as protein folding; has similarity to the mammalian BAG-1 protein Gene:SNO2(YNL334C)|FD-Score:-3.56|P-value:1.85E-4||SGD DESC:Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin Gene:SSO2(YMR183C)|FD-Score:4.41|P-value:5.20E-6||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p Gene:SWA2(YDR320C)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TRP2(YER090W)|FD-Score:5.7|P-value:6.12E-9||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.37|P-value:6.25E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TUB3(YML124C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:VAC7(YNL054W)|FD-Score:-3.22|P-value:6.49E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VPS25(YJR102C)|FD-Score:-3.24|P-value:5.98E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:YAP1(YML007W)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YBR138C(YBR138C_p)|FD-Score:4|P-value:3.18E-5||SGD DESC:Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:4.49|P-value:3.55E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR161W(YDR161W_p)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD) Gene:YGR064W(YGR064W_d)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YKE2(YLR200W)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YMR010W(YMR010W_p)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR010W is not an essential gene; YMR010W mRNA is transcribed with ADI1 Gene:YMR158W-B(YMR158W-B_d)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YNR071C(YNR071C_p)|FD-Score:4.35|P-value:6.91E-6||SGD DESC:Putative aldose 1-epimerase Gene:YOL046C(YOL046C_d)|FD-Score:-3.18|P-value:7.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YPL162C(YPL162C_p)|FD-Score:-4.21|P-value:1.27E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology Gene:YPL216W(YPL216W_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; YPL216W is not an essential gene Gene:YPL225W(YPL225W)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPT31(YER031C)|FD-Score:-3.25|P-value:5.76E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR136W6.066.82E-101.62MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YHR062C5.452.55E-81.62RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YJR076C3.836.52E-50.31CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YER148W3.512.21E-40.20SPT15TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
YKL193C3.324.54E-40.06SDS22Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
YKR038C3.255.68E-40.07KAE1Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription
YPR019W3.197.22E-40.01MCM4Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
YLR208W3.187.37E-40.32SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YGL142C2.860.002140.15GPI10Integral membrane protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the GPI core structure; human PIG-Bp is a functional homolog
YNL216W2.710.003370.03RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
YGR116W2.680.003710.04SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YKL035W2.640.004140.02UGP1UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
YNL263C2.620.004410.04YIF1Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3
YOR319W2.580.005010.12HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YPL075W2.450.007070.00GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR006C5.911.75E-9ICL22-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol
YER090W5.706.12E-9TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YOR221C4.582.32E-6MCT1Predicted malonyl-CoA:ACP transferase, putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling
YDR008C_d4.493.55E-6YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR183C4.415.20E-6SSO2Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p
YKL211C4.376.25E-6TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YNR071C_p4.356.91E-6YNR071C_pPutative aldose 1-epimerase
YDR123C4.102.05E-5INO2Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion
YDR161W_p4.102.08E-5YDR161W_pPutative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD)
YBR138C_p4.003.18E-5YBR138C_pCytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
YPL161C3.963.72E-5BEM4Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
YGR173W3.934.33E-5RBG2Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein
YGL220W3.875.40E-5FRA2Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
YML007W3.865.60E-5YAP1Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YNL316C3.778.08E-5PHA2Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway

GO enrichment analysis for SGTC_1653
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1946.97E-51SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.112676
0.1782.50E-43SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.181818
0.1741.41E-41SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.233333
0.1731.12E-40SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0909091
0.1674.35E-38SGTC_22967971656 126.5 μMChembridge (Fragment library)6665700.0945946
0.1631.35E-36SGTC_2684capsaicin 100.0 μMMiscellaneous15489430.180556
0.1574.53E-34SGTC_1654st012436 41.8 μMTimTec (Natural product derivative library)43231320.121622
0.1574.61E-34SGTC_1100850-0284 111.6 μMChemDiv (Drug-like library)39062650.0625
0.1578.25E-34SGTC_507nimesulide 162.0 μMMiscellaneous44950.134328
0.1532.01E-32SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0895522
0.1532.52E-32SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.0923077
0.1533.14E-32SGTC_18815652484 16.0 μMMiscellaneous22551290.101266TRP & mitochondrial translation
0.1534.26E-32SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.155172
0.1521.05E-31SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.113924
0.1506.25E-31SGTC_22037227154 166.4 μMChembridge (Fragment library)8963040.155172

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1770st04388466.3 μM0.519231670149TimTec (Natural product derivative library)301.767443.5502
SGTC_1753st04392564 μM0.5670181TimTec (Natural product derivative library)313.413963.42703
SGTC_10204335-096193.3 μM0.407407669075ChemDiv (Drug-like library)267.322382.91302
SGTC_1835st00866649.2 μM0.396226690455TimTec (Natural product derivative library)306.186524.2301
SGTC_23117694451200 μM0.3584912163142Chembridge (Fragment library)205.2531.31602
SGTC_3113912349849.47 μM0.3389838897275Chembridge (Drug-like library)308.417363.80812
SGTC_2835900658871.43 μM0.3333332345538Chembridge (Drug-like library)300.782683.27912
SGTC_2994905352071.43 μM0.3333334647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion
SGTC_14464239-019062 μM0.3283581340085ChemDiv (Drug-like library)379.495263.87414
SGTC_3177910750649.47 μM0.3225818897194Chembridge (Drug-like library)314.809263.76512ERG2