st012939

2,3,4,6,7,8,9,9a-octahydro-1H-quinolizin-1-ylmethanol; cobalt; nitric acid; hexahydrate

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1657
Screen concentration 43.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 24207257
SMILES C1CCN2CCCC(C2C1)CO.[N+](=O)(O)[O-].[N+](=O)(O)[O-].O.O.O.O.O.O.[Co]
Standardized SMILES OCC1CCCN2CCCCC12
Molecular weight 462.3145
ALogP -2.48
H-bond donor count 9
H-bond acceptor count 14
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.35
% growth inhibition (Hom. pool) -2.51


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 24207257
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:3.24|P-value:6.06E-4|Clearance:0.51||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:ORC6(YHR118C)|FD-Score:3.14|P-value:8.49E-4|Clearance:0.51||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:POP3(YNL282W)|FD-Score:-3.34|P-value:4.15E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RAT1(YOR048C)|FD-Score:-3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:SEC15(YGL233W)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.51||SGD DESC:Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase Gene:ALG14(YBR070C)|FD-Score:3.24|P-value:6.06E-4|Clearance:0.51||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:ORC6(YHR118C)|FD-Score:3.14|P-value:8.49E-4|Clearance:0.51||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:POP3(YNL282W)|FD-Score:-3.34|P-value:4.15E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RAT1(YOR048C)|FD-Score:-3.63|P-value:1.44E-4|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:SEC15(YGL233W)|FD-Score:3.22|P-value:6.41E-4|Clearance:0.51||SGD DESC:Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 24207257
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AGC1(YPR021C)|FD-Score:3.83|P-value:6.52E-5||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ARK1(YNL020C)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ATC1(YDR184C)|FD-Score:-3.73|P-value:9.59E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:BUD19(YJL188C_d)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAP1(YKL007W)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CDC10(YCR002C)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CDC26(YFR036W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COX9(YDL067C)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:GPR1(YDL035C)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HXT14(YNL318C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:JNM1(YMR294W)|FD-Score:4.92|P-value:4.25E-7||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LIA1(YJR070C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MET22(YOL064C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MGA2(YIR033W)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MNT4(YNR059W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:PRY1(YJL079C)|FD-Score:-3.15|P-value:8.11E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:QRI5(YLR204W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD17(YOR368W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RPN4(YDL020C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS12(YOR369C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RRD1(YIL153W)|FD-Score:3.93|P-value:4.34E-5||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SIS2(YKR072C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKG6(YHR149C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p Gene:SKN7(YHR206W)|FD-Score:6.87|P-value:3.25E-12||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SMP1(YBR182C)|FD-Score:4.08|P-value:2.21E-5||SGD DESC:Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication Gene:SNF2(YOR290C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNF5(YBR289W)|FD-Score:-4.05|P-value:2.53E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:STO1(YMR125W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SYF2(YGR129W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TDA11(YHR159W_p)|FD-Score:-3.09|P-value:9.91E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TDA5(YLR426W_p)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TGL5(YOR081C)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:THI22(YPR121W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis Gene:TRM10(YOL093W)|FD-Score:3.95|P-value:3.99E-5||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:UBX2(YML013W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UMP1(YBR173C)|FD-Score:4.4|P-value:5.36E-6||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:VMA13(YPR036W)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAP1(YML007W)|FD-Score:-4.78|P-value:8.63E-7||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YAR047C(YAR047C_d)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YCK3(YER123W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDL032W(YDL032W_d)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YJR107W(YJR107W_p)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Putative lipase Gene:YKU70(YMR284W)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YMR160W(YMR160W_p)|FD-Score:-3.91|P-value:4.62E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR265C(YMR265C_p)|FD-Score:5.86|P-value:2.30E-9||SGD DESC:Putative protein of unknown function Gene:YOL047C(YOL047C_p)|FD-Score:-4.29|P-value:9.00E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR072W(YOR072W_d)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPR015C(YPR015C_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YSC84(YHR016C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AGC1(YPR021C)|FD-Score:3.83|P-value:6.52E-5||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ARK1(YNL020C)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ATC1(YDR184C)|FD-Score:-3.73|P-value:9.59E-5||SGD DESC:Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress Gene:BUD19(YJL188C_d)|FD-Score:-3.39|P-value:3.44E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CAP1(YKL007W)|FD-Score:3.74|P-value:9.30E-5||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CDC10(YCR002C)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CDC26(YFR036W)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:COX9(YDL067C)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:GPR1(YDL035C)|FD-Score:-3.31|P-value:4.69E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HXT14(YNL318C)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Protein with similarity to hexose transporter family members, expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:JNM1(YMR294W)|FD-Score:4.92|P-value:4.25E-7||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:LIA1(YJR070C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MET22(YOL064C)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Bisphosphate-3'-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway Gene:MGA2(YIR033W)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MNT4(YNR059W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:PRY1(YJL079C)|FD-Score:-3.15|P-value:8.11E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY2; may be involved in detoxification of hydrophobic compounds; PRY1 has a paralog, PRY2, that arose from the whole genome duplication Gene:QRI5(YLR204W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RAD17(YOR368W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RPN4(YDL020C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS12(YOR369C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog Gene:RRD1(YIL153W)|FD-Score:3.93|P-value:4.34E-5||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SIS2(YKR072C)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SKG6(YHR149C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p Gene:SKN7(YHR206W)|FD-Score:6.87|P-value:3.25E-12||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SMP1(YBR182C)|FD-Score:4.08|P-value:2.21E-5||SGD DESC:Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication Gene:SNF2(YOR290C)|FD-Score:3.13|P-value:8.71E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SNF5(YBR289W)|FD-Score:-4.05|P-value:2.53E-5||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions Gene:STO1(YMR125W)|FD-Score:-3.47|P-value:2.59E-4||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:SYF2(YGR129W)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TDA11(YHR159W_p)|FD-Score:-3.09|P-value:9.91E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele Gene:TDA5(YLR426W_p)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TGL5(YOR081C)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate Gene:THI22(YPR121W)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis Gene:TRM10(YOL093W)|FD-Score:3.95|P-value:3.99E-5||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:UBX2(YML013W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:UMP1(YBR173C)|FD-Score:4.4|P-value:5.36E-6||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:VMA13(YPR036W)|FD-Score:3.9|P-value:4.72E-5||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:YAP1(YML007W)|FD-Score:-4.78|P-value:8.63E-7||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YAR047C(YAR047C_d)|FD-Score:3.13|P-value:8.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YCK3(YER123W)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDL032W(YDL032W_d)|FD-Score:-3.51|P-value:2.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene Gene:YDR222W(YDR222W_p)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YJR107W(YJR107W_p)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Putative lipase Gene:YKU70(YMR284W)|FD-Score:3.72|P-value:9.92E-5||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YMR160W(YMR160W_p)|FD-Score:-3.91|P-value:4.62E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YMR265C(YMR265C_p)|FD-Score:5.86|P-value:2.30E-9||SGD DESC:Putative protein of unknown function Gene:YOL047C(YOL047C_p)|FD-Score:-4.29|P-value:9.00E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YOR072W(YOR072W_d)|FD-Score:-4.18|P-value:1.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPR015C(YPR015C_p)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YSC84(YHR016C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR070C3.246.06E-40.51ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YGL233W3.226.41E-40.51SEC15Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
YHR118C3.148.49E-40.51ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YGL061C2.630.004230.02DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YOR340C2.610.004470.12RPA43RNA polymerase I subunit A43
YGR198W2.500.006290.00YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YJR076C2.490.006340.02CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YGL111W2.470.006760.01NSA1Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
YGL074C_d2.460.006940.01YGL074C_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
YJL173C2.450.007110.04RFA3Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YGR024C2.410.007930.04THG1tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
YIL109C2.370.008800.00SEC24Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb2p and Sfb3p
YOR203W_d2.370.008870.07YOR203W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation
YIL026C2.300.010600.02IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YJL054W2.280.011200.03TIM54Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR206W6.873.25E-12SKN7Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
YMR265C_p5.862.30E-9YMR265C_pPutative protein of unknown function
YMR294W4.924.25E-7JNM1Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
YIR033W4.691.35E-6MGA2ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting
YDL067C4.671.48E-6COX9Subunit VIIa of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YOR081C4.523.11E-6TGL5Bifunctional enzyme with triacylglycerol lipase and lysophosphatidic acid acyltransferase activity; involved in triacylglycerol mobilization; localizes to lipid particles; potential Cdc28p substrate
YBR173C4.405.36E-6UMP1Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly
YBR182C4.082.21E-5SMP1Putative transcription factor of the MADS-box family; involved in regulating the response to osmotic stress; SMP1 has a paralog, RLM1, that arose from the whole genome duplication
YLR426W_p4.032.81E-5TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YOL093W3.953.99E-5TRM10tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress
YIL153W3.934.34E-5RRD1Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YPR036W3.904.72E-5VMA13Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
YPR021C3.836.52E-5AGC1Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis
YKL007W3.749.30E-5CAP1Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
YMR284W3.729.92E-5YKU70Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair

GO enrichment analysis for SGTC_1657
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0985.67E-14SGTC_22226885208 172.1 μMChembridge (Fragment library)6095140.0188679
0.0961.69E-13SGTC_1470k295-0292 231.0 μMChemDiv (Drug-like library)50456120.0606061
0.0913.03E-12SGTC_27885282734 71.4 μMChembridge (Drug-like library)743706NA
0.0732.23E-8SGTC_2695st077686 52.0 μMTimTec (Natural product derivative library)28760920.0985916
0.0691.27E-7SGTC_21335320837 200.0 μMChembridge (Fragment library)28413550.0483871ERG2
0.0681.66E-7SGTC_22597964009 200.0 μMChembridge (Fragment library)3131340.0192308superoxide
0.0681.81E-7SGTC_511blebbistatin 171.0 μMICCB bioactive library34769860.0645161DNA intercalators
0.0682.05E-7SGTC_30259087895 49.5 μMChembridge (Drug-like library)17173641NAPDR1
0.0656.97E-7SGTC_26682',4'-dihydroxychalcone 15.1 μMTimTec (Pure natural product library)53572180.0196078
0.0647.70E-7SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.015873
0.0648.75E-7SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.0196078
0.0612.30E-6SGTC_20885278280 127.4 μMChembridge (Fragment library)7915020.0175439
0.0612.32E-6SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0227273
0.0603.30E-6SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0204082
0.0603.58E-6SGTC_14173966-0327 90.4 μMChemDiv (Drug-like library)6511180.0819672

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1638st00837060.6 μM0.41304324207134TimTec (Natural product derivative library)325.830363.46524ergosterol biosynthesis
SGTC_1629st00508544.9 μM0.38297924207091TimTec (Natural product derivative library)445.334994.49704RPP1 & pyrimidine depletion
SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride61.8 μM0.38297917037758TimTec (Natural product derivative library)323.857544.19413ergosterol biosynthesis
SGTC_1660st01296028.1 μM0.38297924207266TimTec (Natural product derivative library)580.4471550.052815iron homeostasis
SGTC_1797st04869556.8 μM0.367347592262TimTec (Natural product derivative library)352.266084.07403NEO1
SGTC_1991st0730008.92 μM0.2857144301969TimTec (Natural product derivative library)466.57415.92105NEO1
SGTC_449xestospongin c11.19 μM0.2631585311502ICCB bioactive library446.70886.5504NEO1-PIK1
SGTC_1969st06947940.1 μM0.2432433895485TimTec (Natural product derivative library)498.948895.96209
SGTC_1658st01294420 μM0.22916724207260TimTec (Natural product derivative library)499.334675-2.601915iron homeostasis
SGTC_1682st012968530 nM0.22916724207270TimTec (Natural product derivative library)376.766681.71635