st013063

ethyl (2E)-2-(1,3-benzodioxol-5-ylmethylidene)-3-oxobutanoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1661
Screen concentration 9.7 μM
Source TimTec (Natural product derivative library)
PubChem CID 721395
SMILES CCOC(=O)C(=CC1=CC2=C(C=C1)OCO2)C(=O)C
Standardized SMILES CCOC(=O)C(=Cc1ccc2OCOc2c1)C(=O)C
Molecular weight 262.258
ALogP 1.96
H-bond donor count 0
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.47
% growth inhibition (Hom. pool) 7.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 721395
Download HIP data (tab-delimited text)  (excel)
Gene:ASA1(YPR085C)|FD-Score:13|P-value:9.26E-39|Clearance:3.94||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CCT3(YJL014W)|FD-Score:3.74|P-value:9.04E-5|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:5.26|P-value:7.29E-8|Clearance:1.12||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CCT8(YJL008C)|FD-Score:4.14|P-value:1.77E-5|Clearance:0.12||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CFT1(YDR301W)|FD-Score:-3.78|P-value:7.84E-5|Clearance:0||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:CLP1(YOR250C)|FD-Score:-3.27|P-value:5.33E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:HYP2(YEL034W)|FD-Score:4.01|P-value:3.01E-5|Clearance:0.27||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:NSE3(YDR288W)|FD-Score:-3.88|P-value:5.25E-5|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:RAP1(YNL216W)|FD-Score:14.2|P-value:3.32E-46|Clearance:3.94||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RBA50(YDR527W)|FD-Score:5.3|P-value:5.78E-8|Clearance:0.04||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:9.03|P-value:8.57E-20|Clearance:3.73||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPP1(YHR062C)|FD-Score:16.4|P-value:1.71E-60|Clearance:3.94||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC18(YBR080C)|FD-Score:-3.11|P-value:9.45E-4|Clearance:0||SGD DESC:ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF Gene:TUB1(YML085C)|FD-Score:3.18|P-value:7.43E-4|Clearance:0.18||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP4(YDR324C)|FD-Score:-3.16|P-value:7.88E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YBR190W(YBR190W_d)|FD-Score:-4.29|P-value:9.02E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YJL015C(YJL015C_d)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.48||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:ASA1(YPR085C)|FD-Score:13|P-value:9.26E-39|Clearance:3.94||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CCT3(YJL014W)|FD-Score:3.74|P-value:9.04E-5|Clearance:0.09||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:5.26|P-value:7.29E-8|Clearance:1.12||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CCT8(YJL008C)|FD-Score:4.14|P-value:1.77E-5|Clearance:0.12||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CFT1(YDR301W)|FD-Score:-3.78|P-value:7.84E-5|Clearance:0||SGD DESC:RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF Gene:CLP1(YOR250C)|FD-Score:-3.27|P-value:5.33E-4|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:HYP2(YEL034W)|FD-Score:4.01|P-value:3.01E-5|Clearance:0.27||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:NSE3(YDR288W)|FD-Score:-3.88|P-value:5.25E-5|Clearance:0||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress Gene:RAP1(YNL216W)|FD-Score:14.2|P-value:3.32E-46|Clearance:3.94||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RBA50(YDR527W)|FD-Score:5.3|P-value:5.78E-8|Clearance:0.04||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:9.03|P-value:8.57E-20|Clearance:3.73||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPP1(YHR062C)|FD-Score:16.4|P-value:1.71E-60|Clearance:3.94||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC18(YBR080C)|FD-Score:-3.11|P-value:9.45E-4|Clearance:0||SGD DESC:ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian NSF Gene:TUB1(YML085C)|FD-Score:3.18|P-value:7.43E-4|Clearance:0.18||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP4(YDR324C)|FD-Score:-3.16|P-value:7.88E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:YBR190W(YBR190W_d)|FD-Score:-4.29|P-value:9.02E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:YJL015C(YJL015C_d)|FD-Score:3.66|P-value:1.27E-4|Clearance:0.48||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 721395
Download HOP data (tab-delimited text)  (excel)
Gene:AGC1(YPR021C)|FD-Score:7.17|P-value:3.70E-13||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ALD6(YPL061W)|FD-Score:5.26|P-value:7.29E-8||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ARP6(YLR085C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ATP7(YKL016C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCH2(YKR027W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CHS5(YLR330W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CIN1(YOR349W)|FD-Score:12.5|P-value:4.11E-36||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CSM1(YCR086W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DIA1(YMR316W)|FD-Score:5.81|P-value:3.20E-9||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ERG28(YER044C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:FCJ1(YKR016W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:GAL2(YLR081W)|FD-Score:5.22|P-value:9.14E-8||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GLR1(YPL091W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GND1(YHR183W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HOM6(YJR139C)|FD-Score:4.59|P-value:2.27E-6||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:JID1(YPR061C)|FD-Score:4.46|P-value:4.19E-6||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:LAC1(YKL008C)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LST7(YGR057C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MNL2(YLR057W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MOG1(YJR074W)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:NMD4(YLR363C)|FD-Score:-3.11|P-value:9.36E-4||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:OPI3(YJR073C)|FD-Score:5.4|P-value:3.28E-8||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAC2(YER007W)|FD-Score:5.01|P-value:2.70E-7||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PHD1(YKL043W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:RGP1(YDR137W)|FD-Score:-4.03|P-value:2.82E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RNH70(YGR276C)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPL13B(YMR142C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL38(YLR325C)|FD-Score:-3.89|P-value:4.93E-5||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPN4(YDL020C)|FD-Score:8.34|P-value:3.66E-17||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SFB2(YNL049C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p Gene:SWA2(YDR320C)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWD1(YAR003W)|FD-Score:4.28|P-value:9.26E-6||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:THI6(YPL214C)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TSC3(YBR058C-A)|FD-Score:15.5|P-value:1.19E-54||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP6(YFR010W)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UME6(YDR207C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:UPS2(YLR168C)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:URE2(YNL229C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VBA2(YBR293W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS63(YLR261C_d)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS68(YOL129W)|FD-Score:5.38|P-value:3.71E-8||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:VPS71(YML041C)|FD-Score:6.67|P-value:1.26E-11||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAP1(YML007W)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YER079W(YER079W_p)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Putative protein of unknown function Gene:YGL072C(YGL072C_d)|FD-Score:4.51|P-value:3.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR242W(YGR242W_d)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YGR290W(YGR290W_d)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YJL120W(YJL120W_d)|FD-Score:5.76|P-value:4.30E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL147C(YJL147C_p)|FD-Score:4.31|P-value:8.00E-6||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YKL030W(YKL030W_d)|FD-Score:-3.14|P-value:8.41E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKR012C(YKR012C_d)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2 Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR103C(YMR103C_d)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL043C(YNL043C_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL170W(YNL170W_d)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL245W(YPL245W_p)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR089W(YPR089W_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPT6(YLR262C)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:AGC1(YPR021C)|FD-Score:7.17|P-value:3.70E-13||SGD DESC:Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis Gene:ALD6(YPL061W)|FD-Score:5.26|P-value:7.29E-8||SGD DESC:Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress Gene:ARP6(YLR085C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ATP7(YKL016C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCH2(YKR027W)|FD-Score:3.13|P-value:8.68E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CHS5(YLR330W)|FD-Score:3.38|P-value:3.60E-4||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:CIN1(YOR349W)|FD-Score:12.5|P-value:4.11E-36||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CSM1(YCR086W)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DIA1(YMR316W)|FD-Score:5.81|P-value:3.20E-9||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:ERG28(YER044C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Endoplasmic reticulum membrane protein, may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p Gene:FCJ1(YKR016W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Mitochondrial inner membrane protein, ortholog of mammalian mitofilin; involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; also has an essential role in the maintenance of crista junctions and inner membrane architecture, as a component of the mitochondrial inner membrane organizing system (MICOS, MitOS or MINOS), a scaffold-like structure on the IMS side of the inner membrane Gene:GAL2(YLR081W)|FD-Score:5.22|P-value:9.14E-8||SGD DESC:Galactose permease, required for utilization of galactose; also able to transport glucose Gene:GLR1(YPL091W)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GND1(YHR183W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:HOM6(YJR139C)|FD-Score:4.59|P-value:2.27E-6||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:JID1(YPR061C)|FD-Score:4.46|P-value:4.19E-6||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:LAC1(YKL008C)|FD-Score:4.46|P-value:4.01E-6||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LST7(YGR057C)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MNL2(YLR057W)|FD-Score:3.12|P-value:8.90E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MOG1(YJR074W)|FD-Score:3.83|P-value:6.29E-5||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:NMD4(YLR363C)|FD-Score:-3.11|P-value:9.36E-4||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:OPI3(YJR073C)|FD-Score:5.4|P-value:3.28E-8||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAC2(YER007W)|FD-Score:5.01|P-value:2.70E-7||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PHD1(YKL043W)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:RGP1(YDR137W)|FD-Score:-4.03|P-value:2.82E-5||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RNH70(YGR276C)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts Gene:RPL13B(YMR142C)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL38(YLR325C)|FD-Score:-3.89|P-value:4.93E-5||SGD DESC:Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog Gene:RPN4(YDL020C)|FD-Score:8.34|P-value:3.66E-17||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SFB2(YNL049C)|FD-Score:4.62|P-value:1.93E-6||SGD DESC:Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sec24p and Sfb3p Gene:SWA2(YDR320C)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:SWD1(YAR003W)|FD-Score:4.28|P-value:9.26E-6||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:THI6(YPL214C)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TSC3(YBR058C-A)|FD-Score:15.5|P-value:1.19E-54||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP6(YFR010W)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UME6(YDR207C)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:UPS2(YLR168C)|FD-Score:3.73|P-value:9.74E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:URE2(YNL229C)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VBA2(YBR293W)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VPS63(YLR261C_d)|FD-Score:-3.26|P-value:5.62E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:VPS68(YOL129W)|FD-Score:5.38|P-value:3.71E-8||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:VPS71(YML041C)|FD-Score:6.67|P-value:1.26E-11||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAP1(YML007W)|FD-Score:3.84|P-value:6.15E-5||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YER079W(YER079W_p)|FD-Score:3.16|P-value:7.80E-4||SGD DESC:Putative protein of unknown function Gene:YGL072C(YGL072C_d)|FD-Score:4.51|P-value:3.31E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR242W(YGR242W_d)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C Gene:YGR290W(YGR290W_d)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter) Gene:YJL120W(YJL120W_d)|FD-Score:5.76|P-value:4.30E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJL147C(YJL147C_p)|FD-Score:4.31|P-value:8.00E-6||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YKL030W(YKL030W_d)|FD-Score:-3.14|P-value:8.41E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKR012C(YKR012C_d)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2 Gene:YMR052C-A(YMR052C-A_d)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR103C(YMR103C_d)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL043C(YNL043C_d)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W Gene:YNL170W(YNL170W_d)|FD-Score:5.06|P-value:2.14E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL245W(YPL245W_p)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:YPR089W(YPR089W_p)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:YPT6(YLR262C)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C16.401.71E-603.94RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YNL216W14.203.32E-463.94RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
YPR085C13.009.26E-393.94ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YOR207C9.038.57E-203.73RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YDR527W5.305.78E-80.04RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YJL111W5.267.29E-81.12CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YJL008C4.141.77E-50.12CCT8Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YEL034W4.013.01E-50.27HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YJL014W3.749.04E-50.09CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YJL015C_d3.661.27E-40.48YJL015C_dDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
YML085C3.187.43E-40.18TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YJL009W_d2.990.001380.07YJL009W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
YFR037C2.920.001750.02RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YKR008W2.900.001850.09RSC4Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
YMR281W2.810.002450.11GPI12ER membrane protein involved in the second step of glycosylphosphatidylinositol (GPI) anchor assembly, the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A15.501.19E-54TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YOR349W12.504.11E-36CIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
YDL020C8.343.66E-17RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YPR021C7.173.70E-13AGC1Mitochondrial amino acid transporter, acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis
YML041C6.671.26E-11VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YMR316W5.813.20E-9DIA1Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
YJL120W_d5.764.30E-9YJL120W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO
YJR073C5.403.28E-8OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
YOL129W5.383.71E-8VPS68Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria
YPL061W5.267.29E-8ALD6Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
YLR081W5.229.14E-8GAL2Galactose permease, required for utilization of galactose; also able to transport glucose
YFR010W5.161.24E-7UBP6Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance
YML124C5.091.82E-7TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YNL170W_d5.062.14E-7YNL170W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YER007W5.012.70E-7PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl

GO enrichment analysis for SGTC_1661
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4890SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.0886076
0.4214.72E-252SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.178082
0.4137.58E-242SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.180556
0.4095.46E-237SGTC_21515563267 85.4 μMChembridge (Fragment library)53108240.078125
0.4071.16E-232SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.157895
0.3932.33E-217SGTC_120849-0099 9.3 μMChemDiv (Drug-like library)60713880.126761
0.3933.50E-217SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.0882353
0.3912.03E-215SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.142857TSC3-RPN4
0.3875.51E-210SGTC_15852',4'-dihydroxychalcone 10.4 μMTimTec (Pure natural product library)53572180.152542
0.3832.34E-205SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.145161
0.3771.01E-198SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0447761TSC3-RPN4
0.3762.33E-197SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.119403
0.3472.44E-166SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0793651TSC3-RPN4
0.3452.38E-164SGTC_1773st048542 73.6 μMTimTec (Natural product derivative library)32371380.0869565
0.3421.30E-161SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0769231TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1652st01193278 μM0.6666671551076TimTec (Natural product derivative library)245.230742.10905RSC complex & mRNA processing
SGTC_1845st0567533.01 μM0.55375839TimTec (Natural product derivative library)207.182762.09104TSC3-RPN4
SGTC_13661683-011321.9 μM0.479167708623ChemDiv (Drug-like library)224.233121.75925
SGTC_2587piperic acid92.2 μM0.4117655370536Microsource (Natural product library)218.20542.16314
SGTC_1837st05547266.5 μM0.40678676091TimTec (Natural product derivative library)301.380123.68703
SGTC_7991310-0557151 μM0.4038465354492ChemDiv (Drug-like library)252.264683.4703superoxide
SGTC_1651st01192886.1 μM0.396226720198TimTec (Natural product derivative library)232.258421.77414RSC complex & mRNA processing
SGTC_1594piperine70.1 μM0.368421638024TimTec (Pure natural product library)285.337662.86403
SGTC_2683piperine63.03 μM0.368421638024TimTec (Pure natural product library)285.337662.86403
SGTC_1710st03175861.5 μM0.3650795394341TimTec (Natural product derivative library)325.31542.70515
SGTC_14003771-899851.5 μM0.360656670767ChemDiv (Drug-like library)299.321182.72214