st014140

4-(2-amino-3-nitro-5-oxo-4H-pyrano[3,2-c]chromen-4-yl)benzonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1663
Screen concentration 55.4 μM
Source TimTec (Natural product derivative library)
PubChem CID 4207120
SMILES C1=CC=C2C(=C1)C3=C(C(C(=C(O3)N)[N+](=O)[O-])C4=CC=C(C=C4)C#N)C(=O)O2
Standardized SMILES NC1=C(C(C2=C(O1)c3ccccc3OC2=O)c4ccc(cc4)C#N)[N+](=O)[O-]
Molecular weight 361.3077
ALogP 2.31
H-bond donor count 1
H-bond acceptor count 7
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.65
% growth inhibition (Hom. pool) 7.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4207120
Download HIP data (tab-delimited text)  (excel)
Gene:LSG1(YGL099W)|FD-Score:4.97|P-value:3.36E-7|Clearance:1.03||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM5(YER146W)|FD-Score:4.12|P-value:1.92E-5|Clearance:1.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCM3(YEL032W)|FD-Score:3.09|P-value:9.97E-4|Clearance:0.05||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NMD3(YHR170W)|FD-Score:5.41|P-value:3.21E-8|Clearance:1.03||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:UBC1(YDR177W)|FD-Score:-3.14|P-value:8.41E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UTP13(YLR222C)|FD-Score:-3.49|P-value:2.39E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP18(YJL069C)|FD-Score:-3.17|P-value:7.61E-4|Clearance:0||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:UTP4(YDR324C)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript Gene:LSG1(YGL099W)|FD-Score:4.97|P-value:3.36E-7|Clearance:1.03||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM5(YER146W)|FD-Score:4.12|P-value:1.92E-5|Clearance:1.03||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MCM3(YEL032W)|FD-Score:3.09|P-value:9.97E-4|Clearance:0.05||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex Gene:NMD3(YHR170W)|FD-Score:5.41|P-value:3.21E-8|Clearance:1.03||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:UBC1(YDR177W)|FD-Score:-3.14|P-value:8.41E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UTP13(YLR222C)|FD-Score:-3.49|P-value:2.39E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:UTP18(YJL069C)|FD-Score:-3.17|P-value:7.61E-4|Clearance:0||SGD DESC:Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data Gene:UTP4(YDR324C)|FD-Score:-3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4207120
Download HOP data (tab-delimited text)  (excel)
Gene:ADH3(YMR083W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:ADK1(YDR226W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:APD1(YBR151W)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:COG7(YGL005C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUE1(YMR264W)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CUP9(YPL177C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DOT6(YER088C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ECT1(YGR007W)|FD-Score:-3.19|P-value:7.21E-4||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:FRA2(YGL220W)|FD-Score:4.06|P-value:2.41E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:HBS1(YKR084C)|FD-Score:7.35|P-value:9.78E-14||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HOT13(YKL084W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:MCX1(YBR227C)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MSH2(YOL090W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MSH3(YCR092C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MTC2(YKL098W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NAT5(YOR253W)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NUP53(YMR153W)|FD-Score:5.08|P-value:1.88E-7||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:NVJ1(YHR195W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OM14(YBR230C)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPT2(YPR194C)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PAA1(YDR071C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PAP2(YOL115W)|FD-Score:6|P-value:9.67E-10||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PBS2(YJL128C)|FD-Score:4.43|P-value:4.81E-6||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PCI8(YIL071C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PET111(YMR257C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:RBG1(YAL036C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RCK2(YLR248W)|FD-Score:-3.96|P-value:3.69E-5||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RFM1(YOR279C)|FD-Score:3.74|P-value:9.14E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPL13B(YMR142C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SCO2(YBR024W)|FD-Score:4.78|P-value:8.85E-7||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SOL2(YCR073W-A)|FD-Score:5.09|P-value:1.83E-7||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPS18(YNL204C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SUR4(YLR372W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:THI72(YOR192C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:VPS68(YOL129W)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YCR102C(YCR102C_p)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR094W(YDR094W_d)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YDR271C(YDR271C_d)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YJL120W(YJL120W_d)|FD-Score:6.81|P-value:5.02E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKR033C(YKR033C_d)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLL047W(YLL047W_d)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YML018C(YML018C_p)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML082W(YML082W_p)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YMR018W(YMR018W_p)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YOR072W(YOR072W_d)|FD-Score:7.87|P-value:1.76E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR342C(YOR342C_p)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YOR379C(YOR379C_d)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:ADH3(YMR083W)|FD-Score:3.48|P-value:2.54E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:ADK1(YDR226W)|FD-Score:3.34|P-value:4.22E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:APD1(YBR151W)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus Gene:COG7(YGL005C)|FD-Score:-3.27|P-value:5.37E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUE1(YMR264W)|FD-Score:5.17|P-value:1.18E-7||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:CUP9(YPL177C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:DOT6(YER088C)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ECT1(YGR007W)|FD-Score:-3.19|P-value:7.21E-4||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:FRA2(YGL220W)|FD-Score:4.06|P-value:2.41E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:HBS1(YKR084C)|FD-Score:7.35|P-value:9.78E-14||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HOT13(YKL084W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:MCX1(YBR227C)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MSH2(YOL090W)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Protein that forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP Gene:MSH3(YCR092C)|FD-Score:3.26|P-value:5.52E-4||SGD DESC:Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability Gene:MTC2(YKL098W)|FD-Score:4.05|P-value:2.57E-5||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NAT5(YOR253W)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NUP53(YMR153W)|FD-Score:5.08|P-value:1.88E-7||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication Gene:NVJ1(YHR195W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OM14(YBR230C)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Integral mitochondrial outer membrane protein; abundance is decreased in cells grown in glucose relative to other carbon sources; appears to contain 3 alpha-helical transmembrane segments; ORF encodes a 97-basepair intron Gene:OPT2(YPR194C)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PAA1(YDR071C)|FD-Score:3.21|P-value:6.53E-4||SGD DESC:Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication Gene:PAP2(YOL115W)|FD-Score:6|P-value:9.67E-10||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p Gene:PBS2(YJL128C)|FD-Score:4.43|P-value:4.81E-6||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PCI8(YIL071C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PET111(YMR257C)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:RBG1(YAL036C)|FD-Score:4.42|P-value:5.02E-6||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RCK2(YLR248W)|FD-Score:-3.96|P-value:3.69E-5||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RFM1(YOR279C)|FD-Score:3.74|P-value:9.14E-5||SGD DESC:DNA-binding protein required for vegetative repression of middle sporulation genes; specificity factor that directs the Hst1p histone deacetylase to some of the promoters regulated by Sum1p; involved in telomere maintenance Gene:RPL13B(YMR142C)|FD-Score:3.19|P-value:7.00E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RTT101(YJL047C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SCO2(YBR024W)|FD-Score:4.78|P-value:8.85E-7||SGD DESC:Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication Gene:SOL2(YCR073W-A)|FD-Score:5.09|P-value:1.83E-7||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SPS18(YNL204C)|FD-Score:3.15|P-value:8.13E-4||SGD DESC:Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation Gene:SUR4(YLR372W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:THI72(YOR192C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:VPS68(YOL129W)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Vacuolar membrane protein of unknown function involved in vacuolar protein sorting; also detected in the mitochondria Gene:YCR102C(YCR102C_p)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR094W(YDR094W_d)|FD-Score:-3.28|P-value:5.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YDR271C(YDR271C_d)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YJL120W(YJL120W_d)|FD-Score:6.81|P-value:5.02E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKR033C(YKR033C_d)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLL047W(YLL047W_d)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1 Gene:YML018C(YML018C_p)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML082W(YML082W_p)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene Gene:YMR018W(YMR018W_p)|FD-Score:3.72|P-value:9.90E-5||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR172C-A(YMR172C-A_d)|FD-Score:3.22|P-value:6.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YOR072W(YOR072W_d)|FD-Score:7.87|P-value:1.76E-15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR342C(YOR342C_p)|FD-Score:3.86|P-value:5.66E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YOR379C(YOR379C_d)|FD-Score:3.15|P-value:8.21E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR170W5.413.21E-81.03NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YGL099W4.973.36E-71.03LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YER146W4.121.92E-51.03LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YEL032W3.099.97E-40.05MCM3Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
YDL102W3.040.001190.12POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
YOR046C2.910.001800.02DBP5Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p
YOR206W2.890.001910.09NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YJL041W2.800.002570.12NSP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress
YCL017C2.680.003710.03NFS1Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
YDL164C2.650.004050.01CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination
YDL120W2.640.004190.03YFH1Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia
YGL040C2.610.004570.03HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YIL143C2.570.005050.01SSL2Component of RNA polymerase transcription factor TFIIH holoenzyme; has DNA-dependent ATPase/helicase activity and is required, with Rad3p, for unwinding promoter DNA; interacts functionally with TFIIB and has roles in transcription start site selection and in gene looping to juxtapose initiation and termination regions; involved in DNA repair; homolog of human ERCC3
YOR143C2.560.005250.02THI80Thiamine pyrophosphokinase, phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate)
YDR052C2.540.005490.03DBF4Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR072W_d7.871.76E-15YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YKR084C7.359.78E-14HBS1GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay
YJL120W_d6.815.02E-12YJL120W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO
YOL115W6.009.67E-10PAP2Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Trf5p
YBR151W5.181.11E-7APD1Protein of unknown function, required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
YMR264W5.171.18E-7CUE1Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
YHR195W5.151.31E-7NVJ1Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)
YCR073W-A5.091.83E-7SOL2Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
YMR153W5.081.88E-7NUP53FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication
YBR024W4.788.85E-7SCO2Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication
YJL128C4.434.81E-6PBS2MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
YAL036C4.425.02E-6RBG1Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid
YGL220W4.062.41E-5FRA2Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
YKL098W4.052.57E-5MTC2Protein of unknown function; mtc2 is synthetically sick with cdc13-1
YJL047C3.944.00E-5RTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p

GO enrichment analysis for SGTC_1663
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2159.63E-63SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.058823560S ribosome export
0.1871.11E-47SGTC_1664st014217 9.0 μMTimTec (Natural product derivative library)39827820.35135160S ribosome export
0.1624.86E-36SGTC_1983454-2873 16.2 μMChemDiv (Drug-like library)7109570.063291160S ribosome export
0.1584.05E-34SGTC_8103825-7150 143.0 μMChemDiv (Drug-like library)223304110.055555660S ribosome export
0.1541.49E-32SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.10810860S ribosome export
0.1483.81E-30SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.091836760S ribosome export
0.1404.05E-27SGTC_32229128301 49.5 μMChembridge (Drug-like library)49113580.055555660S ribosome export
0.1346.19E-25SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.060975660S ribosome export
0.1321.76E-24SGTC_970535-0701 19.1 μMChemDiv (Drug-like library)28320130.10975660S ribosome export
0.1322.39E-24SGTC_8660495-0101 262.0 μMChemDiv (Drug-like library)6356940.13414660S ribosome export
0.1323.28E-24SGTC_14073909-7909 12.3 μMChemDiv (Drug-like library)44117800.079545560S ribosome export
0.1323.94E-24SGTC_1833914-0051 30.6 μMChemDiv (Drug-like library)15962320.080459860S ribosome export
0.1279.33E-23SGTC_1829berberine 14.4 μMTimTec (Natural product derivative library)23530.067415760S ribosome export
0.1271.40E-22SGTC_11861319-0216 26.0 μMChemDiv (Drug-like library)45651360.0842105
0.1262.15E-22SGTC_13341486-0610 236.0 μMChemDiv (Drug-like library)37423390.033333360S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1662st01405452 μM0.5384622838777TimTec (Natural product derivative library)360.362742.92816TSC3-RPN4
SGTC_1634st00657641 μM0.5362323915356TimTec (Natural product derivative library)427.365823.48518TSC3-RPN4
SGTC_1643st00986620.2 μM0.53156273TimTec (Natural product derivative library)320.298881.92616
SGTC_1668st01491451.6 μM0.52839556TimTec (Natural product derivative library)366.368863.50415RPP1 & pyrimidine depletion
SGTC_1719st03222749.2 μM0.4925373125804TimTec (Natural product derivative library)406.388122.54718
SGTC_1917st06650728.5 μM0.3636363156428TimTec (Natural product derivative library)399.3443063.33218Golgi
SGTC_1664st0142179.02 μM0.3513513982782TimTec (Natural product derivative library)413.1967034.0151660S ribosome export
SGTC_10410206-0046107 μM0.28787950494ChemDiv (Drug-like library)258.315621.79624
SGTC_2035515446394.6 μM0.2794122769623Chembridge (Fragment library)254.283861.48914
SGTC_1693xanthone101.9 μM0.2641517020TimTec (Natural product derivative library)196.201422.96402DNA damage response