st014914

2-amino-4-naphthalen-1-yl-5-oxo-4H-pyrano[3,2-c]chromene-3-carbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1668
Screen concentration 51.6 μM
Source TimTec (Natural product derivative library)
PubChem CID 2839556
SMILES C1=CC=C2C(=C1)C=CC=C2C3C(=C(OC4=C3C(=O)OC5=CC=CC=C54)N)C#N
Standardized SMILES NC1=C(C#N)C(C2=C(O1)c3ccccc3OC2=O)c4cccc5ccccc45
Molecular weight 366.3689
ALogP 3.5
H-bond donor count 1
H-bond acceptor count 5
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.46
% growth inhibition (Hom. pool) 4.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2839556
Download HIP data (tab-delimited text)  (excel)
Gene:ERG27(YLR100W)|FD-Score:3.42|P-value:3.15E-4|Clearance:0.08||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:GLC7(YER133W)|FD-Score:3.8|P-value:7.18E-5|Clearance:0.08||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HRR25(YPL204W)|FD-Score:3.99|P-value:3.28E-5|Clearance:0.19||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MAK16(YAL025C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.28||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:MCM2(YBL023C)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.04||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MCM7(YBR202W)|FD-Score:5.02|P-value:2.56E-7|Clearance:0.35||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NAT2(YGR147C)|FD-Score:3.19|P-value:7.19E-4|Clearance:0.11||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:PTI1(YGR156W)|FD-Score:3.2|P-value:6.91E-4|Clearance:0||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPC17(YJL011C)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.01||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPL15A(YLR029C)|FD-Score:4.67|P-value:1.52E-6|Clearance:0.27||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL32(YBL092W)|FD-Score:3.2|P-value:6.92E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPP0(YLR340W)|FD-Score:4.39|P-value:5.57E-6|Clearance:0.07||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:4.32|P-value:7.67E-6|Clearance:0.33||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SKI6(YGR195W)|FD-Score:3.34|P-value:4.25E-4|Clearance:0.06||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SRP1(YNL189W)|FD-Score:3.23|P-value:6.11E-4|Clearance:0.04||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TFC6(YDR362C)|FD-Score:3.42|P-value:3.07E-4|Clearance:0.01||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:ERG27(YLR100W)|FD-Score:3.42|P-value:3.15E-4|Clearance:0.08||SGD DESC:3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs Gene:GLC7(YER133W)|FD-Score:3.8|P-value:7.18E-5|Clearance:0.08||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HRR25(YPL204W)|FD-Score:3.99|P-value:3.28E-5|Clearance:0.19||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:MAK16(YAL025C)|FD-Score:3.72|P-value:1.00E-4|Clearance:0.28||SGD DESC:Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus Gene:MCM2(YBL023C)|FD-Score:3.28|P-value:5.24E-4|Clearance:0.04||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MCM7(YBR202W)|FD-Score:5.02|P-value:2.56E-7|Clearance:0.35||SGD DESC:Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex Gene:NAT2(YGR147C)|FD-Score:3.19|P-value:7.19E-4|Clearance:0.11||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:PTI1(YGR156W)|FD-Score:3.2|P-value:6.91E-4|Clearance:0||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPC17(YJL011C)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.01||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPL15A(YLR029C)|FD-Score:4.67|P-value:1.52E-6|Clearance:0.27||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL32(YBL092W)|FD-Score:3.2|P-value:6.92E-4|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPP0(YLR340W)|FD-Score:4.39|P-value:5.57E-6|Clearance:0.07||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RPP1(YHR062C)|FD-Score:4.32|P-value:7.67E-6|Clearance:0.33||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SKI6(YGR195W)|FD-Score:3.34|P-value:4.25E-4|Clearance:0.06||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SRP1(YNL189W)|FD-Score:3.23|P-value:6.11E-4|Clearance:0.04||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TFC6(YDR362C)|FD-Score:3.42|P-value:3.07E-4|Clearance:0.01||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2839556
Download HOP data (tab-delimited text)  (excel)
Gene:AMS1(YGL156W)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:BOI2(YER114C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BSC1(YDL037C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:COG7(YGL005C)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR8(YNR028W)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CSR2(YPR030W)|FD-Score:-4.02|P-value:2.88E-5||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CTK3(YML112W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CUR1(YPR158W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DUN1(YDL101C)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:EXO1(YOR033C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FAA3(YIL009W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GZF3(YJL110C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HEH2(YDR458C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HHT2(YNL031C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:LEM3(YNL323W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MRPL33(YMR286W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:PHO85(YPL031C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PRO2(YOR323C)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RTT107(YHR154W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SKY1(YMR216C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:TIM11(YDR322C-A)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:UBC7(YMR022W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:YAR029W(YAR029W_p)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR094W(YDR094W_d)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YDR194W-A(YDR194W-A_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER181C(YER181C_d)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Gene:YJL211C(YJL211C_d)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YLR194C(YLR194C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YML047W-A(YML047W-A_d)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:4.78|P-value:8.88E-7||SGD DESC:Putative protein of unknown function Gene:YOR072W(YOR072W_d)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR111W(YOR111W_p)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Putative protein of unknown function Gene:YOR121C(YOR121C_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL168W(YPL168W_p)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:AMS1(YGL156W)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:BOI2(YER114C)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BSC1(YDL037C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression Gene:COG7(YGL005C)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CPR8(YNR028W)|FD-Score:-3.19|P-value:7.11E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CSR2(YPR030W)|FD-Score:-4.02|P-value:2.88E-5||SGD DESC:Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication Gene:CTK3(YML112W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CUR1(YPR158W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:DUN1(YDL101C)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:EXO1(YOR033C)|FD-Score:3.14|P-value:8.39E-4||SGD DESC:5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress Gene:FAA3(YIL009W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:GZF3(YJL110C)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HEH2(YDR458C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle Gene:HHT2(YNL031C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation Gene:LEM3(YNL323W)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:MRPL33(YMR286W)|FD-Score:3.34|P-value:4.12E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:PHO85(YPL031C)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle Gene:PRO2(YOR323C)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RTT107(YHR154W)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SKY1(YMR216C)|FD-Score:3.26|P-value:5.56E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:TIM11(YDR322C-A)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:UBC7(YMR022W)|FD-Score:-3.46|P-value:2.70E-4||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:YAR029W(YAR029W_p)|FD-Score:3.25|P-value:5.67E-4||SGD DESC:Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBR182C-A(YBR182C-A_p)|FD-Score:3.73|P-value:9.57E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YDR094W(YDR094W_d)|FD-Score:-3.34|P-value:4.12E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2 Gene:YDR194W-A(YDR194W-A_p)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER181C(YER181C_d)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies Gene:YJL211C(YJL211C_d)|FD-Score:-3.12|P-value:9.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2 Gene:YLR194C(YLR194C)|FD-Score:3.11|P-value:9.40E-4||SGD DESC:Structural constituent of the cell wall attached to the plasma membrane by a GPI-anchor; expression is upregulated in response to cell wall stress Gene:YML047W-A(YML047W-A_d)|FD-Score:4.67|P-value:1.48E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:4.78|P-value:8.88E-7||SGD DESC:Putative protein of unknown function Gene:YOR072W(YOR072W_d)|FD-Score:-3.41|P-value:3.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR111W(YOR111W_p)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Putative protein of unknown function Gene:YOR121C(YOR121C_d)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPL168W(YPL168W_p)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR202W5.022.56E-70.35MCM7Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
YLR029C4.671.52E-60.27RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YLR340W4.395.57E-60.07RPP0Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10
YHR062C4.327.67E-60.33RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPL204W3.993.28E-50.19HRR25Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
YER133W3.807.18E-50.08GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YAL025C3.721.00E-40.28MAK16Essential nuclear protein, constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus
YJL011C3.442.94E-40.01RPC17RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress
YDR362C3.423.07E-40.01TFC6One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
YLR100W3.423.15E-40.08ERG273-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
YGR195W3.344.25E-40.06SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YBL023C3.285.24E-40.04MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YNL189W3.236.11E-40.04SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YGR156W3.206.91E-42.80E-4PTI1Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
YBL092W3.206.92E-40.01RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR105W-A_p4.788.88E-7YMR105W-A_pPutative protein of unknown function
YML047W-A_d4.671.48E-6YML047W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR182C-A_p3.739.57E-5YBR182C-A_pPutative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
YPL031C3.671.21E-4PHO85Cyclin-dependent kinase, with ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle
YOR121C_d3.661.28E-4YOR121C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W
YDR458C3.631.39E-4HEH2Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
YDR194W-A_p3.591.66E-4YDR194W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YPR158W3.522.18E-4CUR1Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication
YER181C_d3.472.64E-4YER181C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative data; extensively overlaps a Ty1 LTR; protein product is detected in highly purified mitochondria in high-throughput studies
YNL031C3.423.14E-4HHT2Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation
YNL323W3.413.21E-4LEM3Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane
YER114C3.373.80E-4BOI2Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
YMR286W3.344.12E-4MRPL33Mitochondrial ribosomal protein of the large subunit
YHR154W3.344.14E-4RTT107Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
YIL009W3.304.86E-4FAA3Long chain fatty acyl-CoA synthetase, activates imported fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery

GO enrichment analysis for SGTC_1668
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1403.26E-27SGTC_21925915882 198.5 μMChembridge (Fragment library)28761490.112676RNA processing & uracil transport
0.1261.82E-22SGTC_31189124250 49.5 μMChembridge (Drug-like library)15047860.0886076
0.1094.23E-17SGTC_1471k673-0312 37.0 μMChemDiv (Drug-like library)240998840.045977RNA processing & uracil transport
0.0961.46E-13SGTC_2533agelasine 13.7 μMMicrosource (Natural product library)166677450.046729ERG2
0.0921.34E-12SGTC_468gf-109203x 121.0 μMICCB bioactive library23960.116279amide catabolism
0.0896.11E-12SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)50988840.0649351RPP1 & pyrimidine depletion
0.0898.91E-12SGTC_13853091-4834 14.1 μMChemDiv (Drug-like library)40728140.136986RPP1 & pyrimidine depletion
0.0899.30E-12SGTC_1983st071813 46.9 μMTimTec (Natural product derivative library)118385950.168539RPP1 & pyrimidine depletion
0.0871.65E-11SGTC_31679103034 49.5 μMChembridge (Drug-like library)51278590.0897436RPP1 & pyrimidine depletion
0.0872.51E-11SGTC_22707924575 90.9 μMChembridge (Fragment library)12621350.0684932RPP1 & pyrimidine depletion
0.0864.32E-11SGTC_1926methyl fluorone black 53.6 μMTimTec (Natural product derivative library)727210.111111DNA intercalators
0.0841.06E-10SGTC_2505galangin 7.9 μMMicrosource (Natural product library)52816160.15942RPP1 & pyrimidine depletion
0.0841.20E-10SGTC_30409091894 49.5 μMChembridge (Drug-like library)252369660.0714286DNA intercalators
0.0831.96E-10SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0823529RPP1 & pyrimidine depletion
0.0813.93E-10SGTC_420133-0086 60.7 μMChemDiv (Drug-like library)1629230.114286

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1643st00986620.2 μM0.620693156273TimTec (Natural product derivative library)320.298881.92616
SGTC_1662st01405452 μM0.5806452838777TimTec (Natural product derivative library)360.362742.92816TSC3-RPN4
SGTC_1719st03222749.2 μM0.5806453125804TimTec (Natural product derivative library)406.388122.54718
SGTC_2415st07733357.7 μM0.5396832766255TimTec (Natural product derivative library)346.336161.23426
SGTC_1634st00657641 μM0.5294123915356TimTec (Natural product derivative library)427.365823.48518TSC3-RPN4
SGTC_1663st01414055.4 μM0.54207120TimTec (Natural product derivative library)361.307742.311760S ribosome export
SGTC_1664st0142179.02 μM0.43982782TimTec (Natural product derivative library)413.1967034.0151660S ribosome export
SGTC_1917st06650728.5 μM0.3918923156428TimTec (Natural product derivative library)399.3443063.33218Golgi
SGTC_13681852-0141284 μM0.3650792838987ChemDiv (Drug-like library)281.2843832.3225ERG2
SGTC_2067522462934.08 μM0.3484852835000Chembridge (Fragment library)288.728922.15414