st016611

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1673
Screen concentration 27.3 μM
Source TimTec (Natural product derivative library)
PubChem CID 5226799
SMILES CC1=CC2=C(C=C1)N3CCN(C4C3=C2CCC4)[N+](=O)[O-]
Standardized SMILES Cc1ccc2c(c1)c3CCCC4N(CCn2c34)[N+](=O)[O-]
Molecular weight 271.3144
ALogP 5.51
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.55
% growth inhibition (Hom. pool) 9.12


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5226799
Download HIP data (tab-delimited text)  (excel)
Gene:CDC19(YAL038W)|FD-Score:-4.51|P-value:3.20E-6|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:ESS1(YJR017C)|FD-Score:-3.12|P-value:9.13E-4|Clearance:0||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:GNA1(YFL017C)|FD-Score:3.81|P-value:6.86E-5|Clearance:0.22||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:NOP15(YNL110C)|FD-Score:-5.7|P-value:6.10E-9|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:RPA43(YOR340C)|FD-Score:-5.11|P-value:1.61E-7|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPP0(YLR340W)|FD-Score:-7.98|P-value:7.09E-16|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RSC3(YDR303C)|FD-Score:4.35|P-value:6.68E-6|Clearance:0.38||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:RSP5(YER125W)|FD-Score:-4.54|P-value:2.88E-6|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SCL1(YGL011C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.02||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:TFB3(YDR460W)|FD-Score:-4.12|P-value:1.89E-5|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TFG2(YGR005C)|FD-Score:5.11|P-value:1.60E-7|Clearance:0.38||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:TIF35(YDR429C)|FD-Score:3.27|P-value:5.46E-4|Clearance:0.28||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TPT1(YOL102C)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.05||SGD DESC:tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide Gene:TRZ1(YKR079C)|FD-Score:-3.2|P-value:6.83E-4|Clearance:0||SGD DESC:tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 Gene:TSC13(YDL015C)|FD-Score:4.73|P-value:1.13E-6|Clearance:0.38||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:4.94|P-value:3.99E-7|Clearance:0.38||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP9(YHR196W)|FD-Score:4.52|P-value:3.06E-6|Clearance:0.38||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YKL153W(YKL153W_d)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YPP1(YGR198W)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.38||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene Gene:CDC19(YAL038W)|FD-Score:-4.51|P-value:3.20E-6|Clearance:0||SGD DESC:Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication Gene:ESS1(YJR017C)|FD-Score:-3.12|P-value:9.13E-4|Clearance:0||SGD DESC:Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3 Gene:GNA1(YFL017C)|FD-Score:3.81|P-value:6.86E-5|Clearance:0.22||SGD DESC:Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA Gene:NOP15(YNL110C)|FD-Score:-5.7|P-value:6.10E-9|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:RPA43(YOR340C)|FD-Score:-5.11|P-value:1.61E-7|Clearance:0||SGD DESC:RNA polymerase I subunit A43 Gene:RPP0(YLR340W)|FD-Score:-7.98|P-value:7.09E-16|Clearance:0||SGD DESC:Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10 Gene:RSC3(YDR303C)|FD-Score:4.35|P-value:6.68E-6|Clearance:0.38||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:RSP5(YER125W)|FD-Score:-4.54|P-value:2.88E-6|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SCL1(YGL011C)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.02||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:TFB3(YDR460W)|FD-Score:-4.12|P-value:1.89E-5|Clearance:0||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit Gene:TFG2(YGR005C)|FD-Score:5.11|P-value:1.60E-7|Clearance:0.38||SGD DESC:TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 Gene:TIF35(YDR429C)|FD-Score:3.27|P-value:5.46E-4|Clearance:0.28||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TPT1(YOL102C)|FD-Score:3.57|P-value:1.81E-4|Clearance:0.05||SGD DESC:tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide Gene:TRZ1(YKR079C)|FD-Score:-3.2|P-value:6.83E-4|Clearance:0||SGD DESC:tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 Gene:TSC13(YDL015C)|FD-Score:4.73|P-value:1.13E-6|Clearance:0.38||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:TUB1(YML085C)|FD-Score:4.94|P-value:3.99E-7|Clearance:0.38||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP9(YHR196W)|FD-Score:4.52|P-value:3.06E-6|Clearance:0.38||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YKL153W(YKL153W_d)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant Gene:YPP1(YGR198W)|FD-Score:4.19|P-value:1.40E-5|Clearance:0.38||SGD DESC:Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5226799
Download HOP data (tab-delimited text)  (excel)
Gene:ADH4(YGL256W)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:ALD5(YER073W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:AMS1(YGL156W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:ARG3(YJL088W)|FD-Score:10.4|P-value:1.17E-25||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO1(YDR127W)|FD-Score:5.34|P-value:4.57E-8||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG3(YNR007C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:CAF16(YFL028C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CCW12(YLR110C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHS7(YHR142W)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CKA1(YIL035C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CPR7(YJR032W)|FD-Score:-5.42|P-value:3.06E-8||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CSG2(YBR036C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL81(YIR023W)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIG1(YPL049C)|FD-Score:-4.41|P-value:5.26E-6||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOT6(YER088C)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG3(YLR056W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ETP1(YHL010C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Putative protein of unknown function that is required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences Gene:FKS1(YLR342W)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:FPR1(YNL135C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GCN2(YDR283C)|FD-Score:5.89|P-value:1.97E-9||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:8.52|P-value:8.16E-18||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:8.34|P-value:3.71E-17||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:8.2|P-value:1.19E-16||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEP7(YGL057C)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GGA2(YHR108W)|FD-Score:-3.61|P-value:1.50E-4||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GSH1(YJL101C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:HRT3(YLR097C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:HXT2(YMR011W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IGO1(YNL157W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p Gene:IKS1(YJL057C)|FD-Score:4.77|P-value:9.18E-7||SGD DESC:Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p Gene:JIP3(YLR331C_d)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:LSM12(YHR121W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:LYS1(YIR034C)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MEC3(YLR288C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MNT3(YIL014W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MRPL13(YKR006C)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPS5(YBR251W)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB2(YGR014W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSO1(YNR049C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NOP12(YOL041C)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:NPT1(YOR209C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NUP157(YER105C)|FD-Score:-5.11|P-value:1.58E-7||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication Gene:PAR32(YDL173W)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PAU4(YLR461W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PDH1(YPR002W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PDR1(YGL013C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PHO8(YDR481C)|FD-Score:7.33|P-value:1.16E-13||SGD DESC:Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN Gene:PHO84(YML123C)|FD-Score:4.46|P-value:4.13E-6||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO88(YBR106W)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PKR1(YMR123W)|FD-Score:4.34|P-value:7.26E-6||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PYC2(YBR218C)|FD-Score:-5.3|P-value:5.83E-8||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAS2(YNL098C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RAV1(YJR033C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCN1(YKL159C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:RDH54(YBR073W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RPL12B(YDR418W)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL20B(YOR312C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL21A(YBR191W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPL41B(YDL133C-A)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.82|P-value:7.08E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS9A(YPL081W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:RSM22(YKL155C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTT107(YHR154W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:RTT109(YLL002W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SFL1(YOR140W)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SHB17(YKR043C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SNN1(YNL086W_p)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes Gene:SNZ2(YNL333W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SPT4(YGR063C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRT1(YMR101C)|FD-Score:4.29|P-value:9.12E-6||SGD DESC:Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase Gene:SSD1(YDR293C)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SYS1(YJL004C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TEF4(YKL081W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:TIM11(YDR322C-A)|FD-Score:-4.17|P-value:1.50E-5||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TKL1(YPR074C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:14.8|P-value:9.49E-50||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:9.34|P-value:4.77E-21||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:20.2|P-value:1.12E-90||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:17.1|P-value:3.81E-66||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:11.4|P-value:3.33E-30||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBC11(YOR339C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:UBP5(YER144C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:UFO1(YML088W)|FD-Score:6.79|P-value:5.58E-12||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VMA7(YGR020C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VMA9(YCL005W-A)|FD-Score:4.95|P-value:3.71E-7||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS36(YLR417W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBL094C(YBL094C_d)|FD-Score:6.25|P-value:2.07E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YBR221W-A(YBR221W-A_p)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL042W(YCL042W_p)|FD-Score:4.59|P-value:2.27E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR008C(YDR008C_d)|FD-Score:15.7|P-value:4.78E-56||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR018C(YDR018C_p)|FD-Score:4.91|P-value:4.51E-7||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR169C-A(YDR169C-A_p)|FD-Score:5.69|P-value:6.24E-9||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER066C-A(YER066C-A_d)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YER119C-A(YER119C-A_d)|FD-Score:-5.81|P-value:3.19E-9||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL117W(YGL117W_p)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Putative protein of unknown function Gene:YHL008C(YHL008C_p)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR007C-A(YHR007C-A_p)|FD-Score:4.06|P-value:2.41E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL027C(YJL027C_p)|FD-Score:-5.94|P-value:1.47E-9||SGD DESC:Putative protein of unknown function Gene:YJL055W(YJL055W_p)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YKL118W(YKL118W_d)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR047W(YKR047W_d)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YKR104W(YKR104W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR030W(YLR030W_p)|FD-Score:-3.73|P-value:9.53E-5||SGD DESC:Putative protein of unknown function Gene:YLR111W(YLR111W_d)|FD-Score:5.95|P-value:1.38E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR236C(YLR236C_d)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR254C(YMR254C_d)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR064C(YNR064C)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOL073C(YOL073C_p)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YPQ1(YOL092W_p)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YTP1(YNL237W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZAP1(YJL056C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ADH4(YGL256W)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:ALD5(YER073W)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:AMS1(YGL156W)|FD-Score:3.16|P-value:7.76E-4||SGD DESC:Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway Gene:ARG3(YJL088W)|FD-Score:10.4|P-value:1.17E-25||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO1(YDR127W)|FD-Score:5.34|P-value:4.57E-8||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG3(YNR007C)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p Gene:CAF16(YFL028C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator Gene:CCW12(YLR110C)|FD-Score:3.49|P-value:2.44E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHS7(YHR142W)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:CKA1(YIL035C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CPR7(YJR032W)|FD-Score:-5.42|P-value:3.06E-8||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CSG2(YBR036C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:4.17|P-value:1.50E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL81(YIR023W)|FD-Score:3.14|P-value:8.48E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIG1(YPL049C)|FD-Score:-4.41|P-value:5.26E-6||SGD DESC:MAP kinase-responsive inhibitor of the Ste12p transcription factor, involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p Gene:DOT6(YER088C)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG3(YLR056W)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase Gene:ETP1(YHL010C)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Putative protein of unknown function that is required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences Gene:FKS1(YLR342W)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling Gene:FPR1(YNL135C)|FD-Score:3.21|P-value:6.67E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:GCN2(YDR283C)|FD-Score:5.89|P-value:1.97E-9||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:8.52|P-value:8.16E-18||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:8.34|P-value:3.71E-17||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:8.2|P-value:1.19E-16||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEP7(YGL057C)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GGA2(YHR108W)|FD-Score:-3.61|P-value:1.50E-4||SGD DESC:Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin Gene:GSH1(YJL101C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:HRT3(YLR097C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:HXT2(YMR011W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IGO1(YNL157W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p Gene:IKS1(YJL057C)|FD-Score:4.77|P-value:9.18E-7||SGD DESC:Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p Gene:JIP3(YLR331C_d)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2 Gene:LSM12(YHR121W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:LYS1(YIR034C)|FD-Score:4.39|P-value:5.57E-6||SGD DESC:Saccharopine dehydrogenase (NAD+, L-lysine-forming), catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity Gene:MEC3(YLR288C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1 Gene:MNT3(YIL014W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MRPL13(YKR006C)|FD-Score:-3.52|P-value:2.14E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, not essential for mitochondrial translation Gene:MRPS5(YBR251W)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSB2(YGR014W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:MSO1(YNR049C)|FD-Score:4.06|P-value:2.45E-5||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:NOP12(YOL041C)|FD-Score:5.16|P-value:1.21E-7||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:NPT1(YOR209C)|FD-Score:3.21|P-value:6.54E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NUP157(YER105C)|FD-Score:-5.11|P-value:1.58E-7||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication Gene:PAR32(YDL173W)|FD-Score:5.59|P-value:1.13E-8||SGD DESC:Putative protein of unknown function; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; PAR32 is not an essential gene Gene:PAU4(YLR461W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PDH1(YPR002W)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Mitochondrial protein that participates in respiration, induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate Gene:PDR1(YGL013C)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PHO8(YDR481C)|FD-Score:7.33|P-value:1.16E-13||SGD DESC:Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN Gene:PHO84(YML123C)|FD-Score:4.46|P-value:4.13E-6||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PHO88(YBR106W)|FD-Score:3.73|P-value:9.67E-5||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PKR1(YMR123W)|FD-Score:4.34|P-value:7.26E-6||SGD DESC:V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress Gene:PYC2(YBR218C)|FD-Score:-5.3|P-value:5.83E-8||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAS2(YNL098C)|FD-Score:-3.57|P-value:1.80E-4||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RAV1(YJR033C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCN1(YKL159C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region Gene:RDH54(YBR073W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:DNA-dependent ATPase; stimulates strand exchange by modifying the topology of double-stranded DNA; involved in recombinational repair of DNA double-strand breaks during mitosis and meiosis; proposed to be involved in crossover interference; interacts with Dmc1p; stimulates Dmc1p and Rad51p Gene:RPL12B(YDR418W)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL20B(YOR312C)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication Gene:RPL21A(YBR191W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPL41B(YDL133C-A)|FD-Score:4.04|P-value:2.64E-5||SGD DESC:Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.82|P-value:7.08E-7||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS9A(YPL081W)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication Gene:RSM22(YKL155C)|FD-Score:3.14|P-value:8.46E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase Gene:RTT107(YHR154W)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:RTT109(YLL002W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SFL1(YOR140W)|FD-Score:-3.3|P-value:4.79E-4||SGD DESC:Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p Gene:SHB17(YKR043C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SNN1(YNL086W_p)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes Gene:SNZ2(YNL333W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SPT4(YGR063C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SRT1(YMR101C)|FD-Score:4.29|P-value:9.12E-6||SGD DESC:Cis-prenyltransferase involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase Gene:SSD1(YDR293C)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SYS1(YJL004C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TEF4(YKL081W)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:TIM11(YDR322C-A)|FD-Score:-4.17|P-value:1.50E-5||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TKL1(YPR074C)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:14.8|P-value:9.49E-50||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:9.34|P-value:4.77E-21||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:20.2|P-value:1.12E-90||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:17.1|P-value:3.81E-66||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:11.4|P-value:3.33E-30||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBC11(YOR339C)|FD-Score:3.94|P-value:4.00E-5||SGD DESC:Ubiquitin-conjugating enzyme most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2 Gene:UBP5(YER144C)|FD-Score:4.13|P-value:1.83E-5||SGD DESC:Putative ubiquitin-specific protease, closest paralog of Doa4p but has no functional overlap; concentrates at the bud neck Gene:UFO1(YML088W)|FD-Score:6.79|P-value:5.58E-12||SGD DESC:F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation Gene:VMA7(YGR020C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VMA9(YCL005W-A)|FD-Score:4.95|P-value:3.71E-7||SGD DESC:Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis Gene:VPS36(YLR417W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:YAR035C-A(YAR035C-A_p)|FD-Score:3.99|P-value:3.29E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; predicted to have a role in cell budding based on computational guilt by association analysis Gene:YBL094C(YBL094C_d)|FD-Score:6.25|P-value:2.07E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W Gene:YBR221W-A(YBR221W-A_p)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YCL042W(YCL042W_p)|FD-Score:4.59|P-value:2.27E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YDR008C(YDR008C_d)|FD-Score:15.7|P-value:4.78E-56||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR018C(YDR018C_p)|FD-Score:4.91|P-value:4.51E-7||SGD DESC:Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication Gene:YDR169C-A(YDR169C-A_p)|FD-Score:5.69|P-value:6.24E-9||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER066C-A(YER066C-A_d)|FD-Score:4.63|P-value:1.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, partially overlaps uncharacterized ORF YER067W Gene:YER119C-A(YER119C-A_d)|FD-Score:-5.81|P-value:3.19E-9||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2 Gene:YGL117W(YGL117W_p)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Putative protein of unknown function Gene:YHL008C(YHL008C_p)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Putative protein of unknown function, may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR007C-A(YHR007C-A_p)|FD-Score:4.06|P-value:2.41E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL027C(YJL027C_p)|FD-Score:-5.94|P-value:1.47E-9||SGD DESC:Putative protein of unknown function Gene:YJL055W(YJL055W_p)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YKL118W(YKL118W_d)|FD-Score:3.79|P-value:7.49E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR047W(YKR047W_d)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YKR104W(YKR104W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds Gene:YLR030W(YLR030W_p)|FD-Score:-3.73|P-value:9.53E-5||SGD DESC:Putative protein of unknown function Gene:YLR111W(YLR111W_d)|FD-Score:5.95|P-value:1.38E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR236C(YLR236C_d)|FD-Score:3.15|P-value:8.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR254C(YMR254C_d)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNR064C(YNR064C)|FD-Score:5.1|P-value:1.66E-7||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOL073C(YOL073C_p)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; ortholog of fission yeast dsc2 Gene:YPQ1(YOL092W_p)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YTP1(YNL237W)|FD-Score:3.49|P-value:2.37E-4||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:ZAP1(YJL056C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YGR005C5.111.60E-70.38TFG2TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
YML085C4.943.99E-70.38TUB1Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
YDL015C4.731.13E-60.38TSC13Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress
YHR196W4.523.06E-60.38UTP9Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDR303C4.356.68E-60.38RSC3Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p
YGR198W4.191.40E-50.38YPP1Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
YFL017C3.816.86E-50.22GNA1Evolutionarily conserved glucosamine-6-phosphate acetyltransferase required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
YGL011C3.591.66E-40.02SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YOL102C3.571.81E-40.05TPT1tRNA 2'-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide
YKL153W_d3.522.18E-40.25YKL153W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
YDR429C3.275.46E-40.28TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YDL064W2.980.001420.17UBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
YKL108W2.820.002420.02SLD2Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
YPR048W2.800.002580.02TAH18Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance
YML064C2.780.002700.03TEM1GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C20.201.12E-90TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR354W17.103.81E-66TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR008C_d15.704.78E-56YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR007W14.809.49E-50TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YGL026C11.403.33E-30TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YJL088W10.401.17E-25ARG3Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
YER090W9.344.77E-21TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YFR009W8.528.16E-18GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YKR026C8.343.71E-17GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YEL009C8.201.19E-16GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR481C7.331.16E-13PHO8Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN
YML088W6.795.58E-12UFO1F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation
YBL094C_d6.252.07E-10YBL094C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W
YLR111W_d5.951.38E-9YLR111W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YDR283C5.891.97E-9GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control

GO enrichment analysis for SGTC_1673
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5090SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.105263
0.4990SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.105882
0.4990SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.105263
0.4920SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.129412
0.4860SGTC_18825615643 20.0 μMMiscellaneous22530750.0909091TRP & mitochondrial translation
0.4840SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.030303
0.4800SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.076087
0.4720SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.0481928
0.4618.33E-308SGTC_24885268135 45.7 μMMiscellaneous12703560.0540541
0.4511.97E-293SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.112676
0.4481.66E-289SGTC_18815652484 16.0 μMMiscellaneous22551290.130952TRP & mitochondrial translation
0.4485.99E-289SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.12987
0.4462.93E-286SGTC_24785763493 32.3 μMMiscellaneous13655760.0641026
0.4441.98E-284SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.08
0.4442.36E-284SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0571429TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1672st0165986.51 μM0.7272734665860TimTec (Natural product derivative library)339.43147.21303endomembrane recycling
SGTC_1671st01659674.5 μM0.6545452829167TimTec (Natural product derivative library)268.35353.16701
SGTC_2699st07770454.6 μM0.5625645852TimTec (Natural product derivative library)366.49983.13103endomembrane recycling
SGTC_2695st07768652 μM0.5538462876092TimTec (Natural product derivative library)356.481763.68103
SGTC_1811447-16448.07 μM0.439394534600ChemDiv (Drug-like library)401.383066.42201endomembrane recycling
SGTC_13943474-000165.7 μM0.2878795017666ChemDiv (Drug-like library)229.340642.46520endomembrane recycling
SGTC_2694st07768581.9 μM0.2753622838096TimTec (Natural product derivative library)244.33212.80832endomembrane recycling
SGTC_2696st07769174.32 μM0.253968757133TimTec (Natural product derivative library)200.279542.83611
SGTC_1952st07703664.1 μM0.2328772937007TimTec (Natural product derivative library)258.315623.01412
SGTC_1885st05754699.9 μM0.230769702008TimTec (Natural product derivative library)200.236482.18321