st024049

methyl (4Z)-4-[(3-hydroxyphenyl)methylidene]-2-methyl-5-oxo-1-(2-phenylethyl)pyrrole-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1685
Screen concentration 53.1 μM
Source TimTec (Natural product derivative library)
PubChem CID 1941139
SMILES CC1=C(C(=CC2=CC(=CC=C2)O)C(=O)N1CCC3=CC=CC=C3)C(=O)OC
Standardized SMILES COC(=O)C1=C(C)N(CCc2ccccc2)C(=O)C1=Cc3cccc(O)c3
Molecular weight 363.4064
ALogP 3.35
H-bond donor count 1
H-bond acceptor count 4
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.02
% growth inhibition (Hom. pool) 7.96


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1941139
Download HIP data (tab-delimited text)  (excel)
Gene:ALG13(YGL047W)|FD-Score:3.15|P-value:8.12E-4|Clearance:0||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:CBF5(YLR175W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.32||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:CTF13(YMR094W)|FD-Score:3.1|P-value:9.67E-4|Clearance:0.02||SGD DESC:Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis Gene:DED1(YOR204W)|FD-Score:-3.26|P-value:5.56E-4|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:DOP1(YDR141C)|FD-Score:3.97|P-value:3.60E-5|Clearance:0.32||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:KOG1(YHR186C)|FD-Score:3.15|P-value:8.19E-4|Clearance:0.05||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:RSP5(YER125W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SEC3(YER008C)|FD-Score:-3.11|P-value:9.22E-4|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SNP1(YIL061C)|FD-Score:3.48|P-value:2.55E-4|Clearance:0.32||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:TFB4(YPR056W)|FD-Score:-3.2|P-value:6.77E-4|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TIM50(YPL063W)|FD-Score:3.72|P-value:9.80E-5|Clearance:0.32||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:ALG13(YGL047W)|FD-Score:3.15|P-value:8.12E-4|Clearance:0||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:CBF5(YLR175W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.32||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:CTF13(YMR094W)|FD-Score:3.1|P-value:9.67E-4|Clearance:0.02||SGD DESC:Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis Gene:DED1(YOR204W)|FD-Score:-3.26|P-value:5.56E-4|Clearance:0||SGD DESC:ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility Gene:DOP1(YDR141C)|FD-Score:3.97|P-value:3.60E-5|Clearance:0.32||SGD DESC:Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies Gene:KOG1(YHR186C)|FD-Score:3.15|P-value:8.19E-4|Clearance:0.05||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:RSP5(YER125W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, and ribosome stability; human homolog is involved in Liddle syndrome; mutant tolerates aneuploidy; ubiquitylates Sec23p Gene:SEC3(YER008C)|FD-Score:-3.11|P-value:9.22E-4|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress Gene:SNP1(YIL061C)|FD-Score:3.48|P-value:2.55E-4|Clearance:0.32||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:TFB4(YPR056W)|FD-Score:-3.2|P-value:6.77E-4|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:TIM50(YPL063W)|FD-Score:3.72|P-value:9.80E-5|Clearance:0.32||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1941139
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AFI1(YOR129C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:AIM29(YKR074W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIM45(YPR004C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ATG8(YBL078C)|FD-Score:8.65|P-value:2.66E-18||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:CKI1(YLR133W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CPR4(YCR069W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CST26(YBR042C)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:DSF2(YBR007C_p)|FD-Score:5.67|P-value:7.13E-9||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FAR1(YJL157C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:HXK1(YFR053C)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IDH2(YOR136W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:ILV6(YCL009C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IMD3(YLR432W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IRC24(YIR036C_p)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:MOD5(YOR274W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRPL25(YGR076C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MUP3(YHL036W)|FD-Score:5.8|P-value:3.41E-9||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NPT1(YOR209C)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NTE1(YML059C)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:OAF1(YAL051W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:PFK27(YOL136C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A Gene:REE1(YJL217W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle Gene:RFX1(YLR176C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RPA12(YJR063W)|FD-Score:6.58|P-value:2.40E-11||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPS8A(YBL072C)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SDH1(YKL148C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SMI1(YGR229C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNX4(YJL036W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SSA3(YBL075C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:9.22|P-value:1.49E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URE2(YNL229C)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:YCR102C(YCR102C_p)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR134C(YDR134C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YER084W(YER084W_p)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YFR020W(YFR020W_p)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR093W(YGR093W_p)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR032W(YKR032W_d)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML116W-A(YML116W-A_d)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function Gene:YNR065C(YNR065C_p)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YPL077C(YPL077C_p)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPT10(YBR264C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Gene:YSP2(YDR326C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ADE1(YAR015W)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AFI1(YOR129C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:AIM29(YKR074W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:AIM45(YPR004C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ATG8(YBL078C)|FD-Score:8.65|P-value:2.66E-18||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:CKI1(YLR133W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CPR4(YCR069W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CST26(YBR042C)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:DSF2(YBR007C_p)|FD-Score:5.67|P-value:7.13E-9||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:FAR1(YJL157C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:HXK1(YFR053C)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IDH2(YOR136W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:ILV6(YCL009C)|FD-Score:-3.39|P-value:3.51E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IMD3(YLR432W)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IRC24(YIR036C_p)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci Gene:MOD5(YOR274W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRPL25(YGR076C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MUP3(YHL036W)|FD-Score:5.8|P-value:3.41E-9||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NPT1(YOR209C)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:NTE1(YML059C)|FD-Score:3.73|P-value:9.64E-5||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:OAF1(YAL051W)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:PFK27(YOL136C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A Gene:REE1(YJL217W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle Gene:RFX1(YLR176C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RPA12(YJR063W)|FD-Score:6.58|P-value:2.40E-11||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPS8A(YBL072C)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:4.03|P-value:2.81E-5||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:SDH1(YKL148C)|FD-Score:3.41|P-value:3.28E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SMI1(YGR229C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNX4(YJL036W)|FD-Score:3.38|P-value:3.56E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SSA3(YBL075C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:9.22|P-value:1.49E-20||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URE2(YNL229C)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:YCR102C(YCR102C_p)|FD-Score:4.42|P-value:5.04E-6||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR134C(YDR134C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YER084W(YER084W_p)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YFR020W(YFR020W_p)|FD-Score:3.41|P-value:3.23E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR093W(YGR093W_p)|FD-Score:-3.24|P-value:6.04E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR032W(YKR032W_d)|FD-Score:3.93|P-value:4.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML116W-A(YML116W-A_d)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Putative protein of unknown function Gene:YNR065C(YNR065C_p)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YPL077C(YPL077C_p)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPT10(YBR264C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Gene:YSP2(YDR326C)|FD-Score:4.57|P-value:2.42E-6||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR141C3.973.60E-50.32DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
YPL063W3.729.80E-50.32TIM50Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
YLR175W3.482.54E-40.32CBF5Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita
YIL061C3.482.55E-40.32SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YGL047W3.158.12E-40.00ALG13Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress
YHR186C3.158.19E-40.05KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YMR094W3.109.67E-40.02CTF13Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
YLR009W3.080.001030.01RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YPR144C3.070.001060.15NOC4Nucleolar protein, forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits
YLR208W2.920.001770.14SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YHR069C2.780.002730.01RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YBR110W2.770.002810.01ALG1Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
YDR324C2.760.002880.00UTP4Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript
YML069W2.760.002890.01POB3Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
YPR181C2.740.003030.01SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A9.221.49E-20TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YBL078C8.652.66E-18ATG8Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis
YJR063W6.582.40E-11RPA12RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
YHL036W5.803.41E-9MUP3Low affinity methionine permease, similar to Mup1p
YBR007C_p5.677.13E-9DSF2_pDeletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
YDR326C4.572.42E-6YSP2Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
YOR209C4.473.97E-6NPT1Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus
YCR102C_p4.425.04E-6YCR102C_pPutative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family
YPR004C4.241.13E-5AIM45Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response
YKR074W4.161.61E-5AIM29Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss
YNL229C4.092.19E-5URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
YIL153W4.032.81E-5RRD1Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
YPL077C_p4.022.88E-5YPL077C_pPutative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
YKR032W_d3.934.21E-5YKR032W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YFR053C3.924.42E-5HXK1Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1685
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2495.14E-84SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.025TSC3-RPN4
0.2471.25E-82SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.151163TSC3-RPN4
0.2436.35E-80SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.101266TSC3-RPN4
0.2402.09E-78SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0952381TSC3-RPN4
0.2294.76E-71SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0714286TSC3-RPN4
0.2231.64E-67SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.150685TSC3-RPN4
0.2141.92E-62SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0789474TSC3-RPN4
0.2101.12E-59SGTC_1726st035556 14.6 μMTimTec (Natural product derivative library)59194390.123288
0.2056.10E-57SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.105263TSC3-RPN4
0.2033.68E-56SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.146067TSC3-RPN4
0.2001.66E-54SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.0779221TSC3-RPN4
0.1962.04E-52SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.232877TSC3-RPN4
0.1951.39E-51SGTC_1634st006576 41.0 μMTimTec (Natural product derivative library)39153560.0693069TSC3-RPN4
0.1911.31E-49SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.142857TSC3-RPN4
0.1906.29E-49SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.214286TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_24305571206200 μM0.2916671731771Miscellaneous315.409924.34704
SGTC_442cape100 μM0.2898555281787ICCB bioactive library284.306543.57324
SGTC_2351902685872.31 μM0.2769231409300Chembridge (Fragment library)231.250520.87923RNA processing & uracil transport
SGTC_1235benzylparaben21.5 μM0.2741947180ChemDiv (Drug-like library)228.243283.02613
SGTC_1038k292-0204154 μM0.2714294454212ChemDiv (Drug-like library)288.38788413
SGTC_483fpl-64176144 μM0.2676063423ICCB bioactive library347.407044.44813cell wall
SGTC_2897904859812.99 μM0.2676066467404Chembridge (Drug-like library)283.321782.99113
SGTC_2441573094639.09 μM0.265061774275Miscellaneous461.328984.67716
SGTC_21806301957200 μM0.264706845462Chembridge (Fragment library)249.285642.09415RSC complex & mRNA processing
SGTC_23729071633200 μM0.26229521321366Chembridge (Fragment library)199.248422.9822