k015-0017

5-bromo-1-[(4-fluorophenyl)methyl]indole-2,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_169
Screen concentration 16.0 μM
Source ChemDiv (Drug-like library)
PubChem CID 1840002
SMILES C1=CC(=CC=C1CN2C3=C(C=C(C=C3)Br)C(=O)C2=O)F
Standardized SMILES Fc1ccc(CN2C(=O)C(=O)c3cc(Br)ccc23)cc1
Molecular weight 334.1399
ALogP 3.41
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.66
% growth inhibition (Hom. pool) 9.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1840002
Download HIP data (tab-delimited text)  (excel)
Gene:ARB1(YER036C)|FD-Score:-6.17|P-value:3.49E-10|Clearance:0||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:BOS1(YLR078C)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.1||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:BRF1(YGR246C)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.19||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:CLF1(YLR117C)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.01||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins Gene:ECM9(YKR004C)|FD-Score:-3.54|P-value:1.99E-4|Clearance:0||SGD DESC:Non-essential protein of unknown function Gene:IDI1(YPL117C)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:MOB1(YIL106W)|FD-Score:3.43|P-value:3.05E-4|Clearance:0.18||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:RRP1(YDR087C)|FD-Score:4.01|P-value:3.01E-5|Clearance:0.47||SGD DESC:Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles Gene:SMT3(YDR510W)|FD-Score:4.47|P-value:3.95E-6|Clearance:0.47||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SMX3(YPR182W)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.47||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:TSC13(YDL015C)|FD-Score:-6.63|P-value:1.74E-11|Clearance:0||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:UBC9(YDL064W)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:YDR187C(YDR187C_d)|FD-Score:3.43|P-value:3.01E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:ARB1(YER036C)|FD-Score:-6.17|P-value:3.49E-10|Clearance:0||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:BOS1(YLR078C)|FD-Score:3.54|P-value:2.01E-4|Clearance:0.1||SGD DESC:v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi Gene:BRF1(YGR246C)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.19||SGD DESC:TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB Gene:CLF1(YLR117C)|FD-Score:3.44|P-value:2.90E-4|Clearance:0.01||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins Gene:ECM9(YKR004C)|FD-Score:-3.54|P-value:1.99E-4|Clearance:0||SGD DESC:Non-essential protein of unknown function Gene:IDI1(YPL117C)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase), catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability Gene:MOB1(YIL106W)|FD-Score:3.43|P-value:3.05E-4|Clearance:0.18||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:RRP1(YDR087C)|FD-Score:4.01|P-value:3.01E-5|Clearance:0.47||SGD DESC:Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles Gene:SMT3(YDR510W)|FD-Score:4.47|P-value:3.95E-6|Clearance:0.47||SGD DESC:Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2 Gene:SMX3(YPR182W)|FD-Score:4.24|P-value:1.13E-5|Clearance:0.47||SGD DESC:Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F Gene:TSC13(YDL015C)|FD-Score:-6.63|P-value:1.74E-11|Clearance:0||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:UBC9(YDL064W)|FD-Score:-3.51|P-value:2.26E-4|Clearance:0||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:YDR187C(YDR187C_d)|FD-Score:3.43|P-value:3.01E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1840002
Download HOP data (tab-delimited text)  (excel)
Gene:AAH1(YNL141W)|FD-Score:-5.76|P-value:4.11E-9||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:ACB1(YGR037C)|FD-Score:5.38|P-value:3.80E-8||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADK1(YDR226W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ALD3(YMR169C)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:AMD1(YML035C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:ANP1(YEL036C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ANT1(YPR128C)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARO4(YBR249C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:ATG31(YDR022C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:ATG5(YPL149W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:ATG8(YBL078C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BEM2(YER155C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BSC2(YDR275W)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:BUD17(YNR027W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:COG5(YNL051W)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL81(YIR023W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUR3(YHL016C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ECT1(YGR007W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:EGD1(YPL037C)|FD-Score:-4.65|P-value:1.67E-6||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ELP4(YPL101W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:GDB1(YPR184W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GTT3(YEL017W)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HAP5(YOR358W)|FD-Score:5.28|P-value:6.31E-8||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HHO1(YPL127C)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination Gene:HOP2(YGL033W)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HSL1(YKL101W)|FD-Score:-4.28|P-value:9.30E-6||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:HXT1(YHR094C)|FD-Score:-5.65|P-value:7.93E-9||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:IGO2(YHR132W-A)|FD-Score:-3.19|P-value:7.14E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:INP1(YMR204C)|FD-Score:-3.63|P-value:1.39E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IOC3(YFR013W)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC18(YJL037W)|FD-Score:-5.04|P-value:2.38E-7||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC6(YFR043C)|FD-Score:-3.73|P-value:9.73E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-4.02|P-value:2.95E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KDX1(YKL161C)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LRE1(YCL051W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MHO1(YJR008W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MTC5(YDR128W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NBA1(YOL070C)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NHP6A(YPR052C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NUR1(YDL089W)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OPI9(YLR338W_d)|FD-Score:-4.46|P-value:4.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OST5(YGL226C-A)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:OSW1(YOR255W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PBP4(YDL053C)|FD-Score:6.54|P-value:2.99E-11||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PER1(YCR044C)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET20(YPL159C)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PEX3(YDR329C)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PHR1(YOR386W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PIR1(YKL164C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:-3.99|P-value:3.35E-5||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PYC2(YBR218C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:QCR6(YFR033C)|FD-Score:-3.97|P-value:3.57E-5||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RAV1(YJR033C)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RIC1(YLR039C)|FD-Score:-3.79|P-value:7.47E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL27B(YDR471W)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication Gene:RRT2(YBR246W)|FD-Score:5.05|P-value:2.20E-7||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SBE22(YHR103W)|FD-Score:4.83|P-value:6.84E-7||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SET2(YJL168C)|FD-Score:-4.29|P-value:8.87E-6||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SLA2(YNL243W)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo Gene:TKL1(YPR074C)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:-5.3|P-value:5.78E-8||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM71(YHR117W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:TOS2(YGR221C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TUS1(YLR425W)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UTP30(YKR060W)|FD-Score:-4.47|P-value:3.87E-6||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VPS1(YKR001C)|FD-Score:-4.5|P-value:3.43E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS51(YKR020W)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YEL014C(YEL014C_d)|FD-Score:5.02|P-value:2.64E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER091C-A(YER091C-A_d)|FD-Score:-4.12|P-value:1.90E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER158C(YER158C_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YFL063W(YFL063W_d)|FD-Score:6.02|P-value:8.99E-10||SGD DESC:Dubious open reading frame, based on available experimental and comparative sequence data Gene:YGL072C(YGL072C_d)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YKL063C(YKL063C_p)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YLR406C-A(YLR406C-A_p)|FD-Score:4.83|P-value:6.72E-7||SGD DESC:Putative protein of unknown function Gene:YOX1(YML027W)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPR012W(YPR012W_d)|FD-Score:4.87|P-value:5.69E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR063C(YPR063C_p)|FD-Score:-5.84|P-value:2.53E-9||SGD DESC:ER-localized protein of unknown function Gene:YPT6(YLR262C)|FD-Score:-3.91|P-value:4.66E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:AAH1(YNL141W)|FD-Score:-5.76|P-value:4.11E-9||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:ACB1(YGR037C)|FD-Score:5.38|P-value:3.80E-8||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADK1(YDR226W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ALD3(YMR169C)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose Gene:AMD1(YML035C)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:ANP1(YEL036C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ANT1(YPR128C)|FD-Score:-3.15|P-value:8.04E-4||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:ARO4(YBR249C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress Gene:ATG31(YDR022C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Autophagy-specific protein required for autophagosome formation; may form a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; high-copy suppressor of CIK1 deletion Gene:ATG5(YPL149W)|FD-Score:-3.49|P-value:2.39E-4||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:ATG8(YBL078C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Component of autophagosomes and Cvt vesicles; unique ubiquitin-like protein whose conjugation target is the lipid phosphatidylethanolamine (PE); Atg8p-PE is anchored to membranes, is involved in phagophore expansion, and may mediate membrane fusion during autophagosome formation; deconjugation of Atg8p-PE is required for efficient autophagosome biogenesis Gene:BEM2(YER155C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:BSC2(YDR275W)|FD-Score:3.45|P-value:2.81E-4||SGD DESC:Protein of unknown function; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; BSC2 has a paralog, IRC23, that arose from the whole genome duplication Gene:BUD17(YNR027W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:COG5(YNL051W)|FD-Score:-3.44|P-value:2.89E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DAL81(YIR023W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DUR3(YHL016C)|FD-Score:3.13|P-value:8.62E-4||SGD DESC:Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway Gene:ECT1(YGR007W)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:EGD1(YPL037C)|FD-Score:-4.65|P-value:1.67E-6||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ELP4(YPL101W)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity Gene:GDB1(YPR184W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GTT3(YEL017W)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery Gene:HAP5(YOR358W)|FD-Score:5.28|P-value:6.31E-8||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex Gene:HHO1(YPL127C)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination Gene:HOP2(YGL033W)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:HSL1(YKL101W)|FD-Score:-4.28|P-value:9.30E-6||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:HXT1(YHR094C)|FD-Score:-5.65|P-value:7.93E-9||SGD DESC:Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication Gene:IGO2(YHR132W-A)|FD-Score:-3.19|P-value:7.14E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:INP1(YMR204C)|FD-Score:-3.63|P-value:1.39E-4||SGD DESC:Peripheral membrane protein of peroxisomes involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane Gene:IOC3(YFR013W)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC18(YJL037W)|FD-Score:-5.04|P-value:2.38E-7||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRC6(YFR043C)|FD-Score:-3.73|P-value:9.73E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-4.02|P-value:2.95E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KDX1(YKL161C)|FD-Score:5.46|P-value:2.42E-8||SGD DESC:Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p Gene:LRE1(YCL051W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MHO1(YJR008W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility Gene:MTC5(YDR128W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; mtc5 is synthetically sick with cdc13-1; relative distribution to vacuolar membrane punctae decreases upon DNA replication stress Gene:NBA1(YOL070C)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NHP6A(YPR052C)|FD-Score:3.67|P-value:1.19E-4||SGD DESC:High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:NUR1(YDL089W)|FD-Score:-3.23|P-value:6.23E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OPI9(YLR338W_d)|FD-Score:-4.46|P-value:4.18E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OST5(YGL226C-A)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:OSW1(YOR255W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PBP4(YDL053C)|FD-Score:6.54|P-value:2.99E-11||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PER1(YCR044C)|FD-Score:-3.28|P-value:5.24E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PET20(YPL159C)|FD-Score:3.9|P-value:4.83E-5||SGD DESC:Mitochondrial protein, required for respiratory growth under some conditions and for stability of the mitochondrial genome Gene:PEX3(YDR329C)|FD-Score:3.93|P-value:4.30E-5||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:PHR1(YOR386W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:PIR1(YKL164C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication Gene:PTC7(YHR076W)|FD-Score:-3.99|P-value:3.35E-5||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:PYC2(YBR218C)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:QCR6(YFR033C)|FD-Score:-3.97|P-value:3.57E-5||SGD DESC:Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space Gene:RAV1(YJR033C)|FD-Score:3.82|P-value:6.75E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RIC1(YLR039C)|FD-Score:-3.79|P-value:7.47E-5||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RPL27B(YDR471W)|FD-Score:3.32|P-value:4.42E-4||SGD DESC:Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication Gene:RRT2(YBR246W)|FD-Score:5.05|P-value:2.20E-7||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:SBE22(YHR103W)|FD-Score:4.83|P-value:6.84E-7||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SET2(YJL168C)|FD-Score:-4.29|P-value:8.87E-6||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SLA2(YNL243W)|FD-Score:-4.2|P-value:1.31E-5||SGD DESC:Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo Gene:TKL1(YPR074C)|FD-Score:5.11|P-value:1.59E-7||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:-5.3|P-value:5.78E-8||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM71(YHR117W)|FD-Score:3.6|P-value:1.56E-4||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:TOS2(YGR221C)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TUS1(YLR425W)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UTP30(YKR060W)|FD-Score:-4.47|P-value:3.87E-6||SGD DESC:Putative subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VPS1(YKR001C)|FD-Score:-4.5|P-value:3.43E-6||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS20(YMR077C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS51(YKR020W)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YEL014C(YEL014C_d)|FD-Score:5.02|P-value:2.64E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER091C-A(YER091C-A_d)|FD-Score:-4.12|P-value:1.90E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER158C(YER158C_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YFL063W(YFL063W_d)|FD-Score:6.02|P-value:8.99E-10||SGD DESC:Dubious open reading frame, based on available experimental and comparative sequence data Gene:YGL072C(YGL072C_d)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YKL063C(YKL063C_p)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi Gene:YLR406C-A(YLR406C-A_p)|FD-Score:4.83|P-value:6.72E-7||SGD DESC:Putative protein of unknown function Gene:YOX1(YML027W)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication Gene:YPR012W(YPR012W_d)|FD-Score:4.87|P-value:5.69E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene Gene:YPR063C(YPR063C_p)|FD-Score:-5.84|P-value:2.53E-9||SGD DESC:ER-localized protein of unknown function Gene:YPT6(YLR262C)|FD-Score:-3.91|P-value:4.66E-5||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR510W4.473.95E-60.47SMT3Ubiquitin-like protein of the SUMO family, conjugated to lysine residues of target proteins; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; phosphorylated at Ser2
YPR182W4.241.13E-50.47SMX3Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
YDR087C4.013.01E-50.47RRP1Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles
YLR078C3.542.01E-40.10BOS1v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi
YLR117C3.442.90E-40.01CLF1Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
YDR187C_d3.433.01E-40.00YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YIL106W3.433.05E-40.18MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YGR246C3.255.83E-40.19BRF1TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
YCR054C3.050.001130.02CTR86Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
YOR148C3.030.001210.04SPP2Essential protein that promotes the first step of splicing and is required for the final stages of spliceosome maturation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction
YNL247W3.000.001370.11YNL247WCysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
YJL033W2.890.001940.02HCA4DEAD box RNA helicase; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
YDR182W2.860.002100.02CDC1Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
YGR091W2.840.002270.03PRP31Splicing factor, component of the U4/U6-U5 snRNP complex
YNL006W2.810.002480.17LST8Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL053C6.542.99E-11PBP4Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
YFL063W_d6.028.99E-10YFL063W_dDubious open reading frame, based on available experimental and comparative sequence data
YKL161C5.462.42E-8KDX1Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
YGR037C5.383.80E-8ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YOR358W5.286.31E-8HAP5Subunit of the heme-activated, glucose-repressed Hap2/3/4/5 CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
YPR074C5.111.59E-7TKL1Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
YBR246W5.052.20E-7RRT2Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
YGL226C-A5.022.59E-7OST5Zeta subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
YEL014C_d5.022.64E-7YEL014C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPR012W_d4.875.69E-7YPR012W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene
YLR406C-A_p4.836.72E-7YLR406C-A_pPutative protein of unknown function
YHR103W4.836.84E-7SBE22Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth
YML027W4.661.56E-6YOX1Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication
YFR013W4.572.43E-6IOC3Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication
YKL164C4.231.16E-5PIR1O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication

GO enrichment analysis for SGTC_169
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1196.69E-20SGTC_22236903751 107.6 μMChembridge (Fragment library)55988630.151515superoxide
0.0822.46E-10SGTC_7991310-0557 151.0 μMChemDiv (Drug-like library)53544920.078125superoxide
0.0759.76E-9SGTC_162k030-0015 37.8 μMChemDiv (Drug-like library)18099200.229167iron homeostasis
0.0672.37E-7SGTC_22147179297 48.3 μMChembridge (Fragment library)7697440.105263superoxide
0.0672.63E-7SGTC_22016605497 169.9 μMChembridge (Fragment library)6707410.101695ergosterol biosynthesis
0.0673.19E-7SGTC_22176934799 96.1 μMChembridge (Fragment library)6756060.046875TSC3-RPN4
0.0655.84E-7SGTC_8650488-0499 301.0 μMChemDiv (Drug-like library)96346510.047619
0.0631.14E-6SGTC_455paf C-16 10.0 μMMiscellaneous1081560.025974
0.0571.03E-5SGTC_1573oleylamine 1.1 μMTimTec (Pure natural product library)53567890.0188679
0.0561.52E-5SGTC_830170-0301 39.0 μMChemDiv (Drug-like library)30897160.0526316endomembrane recycling
0.0561.65E-5SGTC_9861493-0483 84.0 μMChemDiv (Drug-like library)400790.0615385
0.0561.79E-5SGTC_30549094397 49.5 μMChembridge (Drug-like library)25983540.163934fatty acid desaturase (OLE1)
0.0561.90E-5SGTC_8710574-0004 149.0 μMChemDiv (Drug-like library)95511730.056338
0.0552.10E-5SGTC_21265251174 33.9 μMChembridge (Fragment library)7907220.0192308
0.0552.67E-5SGTC_24215690543 90.7 μMChembridge (Fragment library)33979930.0277778

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1458k015-00346.33 μM0.7948723598957ChemDiv (Drug-like library)352.1303263.61904
SGTC_196k015-002318.11 μM0.6818185211998ChemDiv (Drug-like library)361.146963.10204PDR1
SGTC_544k015-002052.4 μM0.6097563008304ChemDiv (Drug-like library)316.14943.20802
SGTC_1027k015-00258.67 μM0.5714293598955ChemDiv (Drug-like library)361.146963.10204
SGTC_349k015-001424 μM0.5652172196908ChemDiv (Drug-like library)350.594463.87202
SGTC_10483161-030024.5 μM0.558142165101ChemDiv (Drug-like library)266.090722.24102
SGTC_20024023503131 μM0.523814736863Chembridge (Fragment library)193.1744231.4303mitochondrial processes
SGTC_295k015-002731 μM0.483887424ChemDiv (Drug-like library)296.277482.8404PDR1
SGTC_1031k035-000916 μM0.4642865180959ChemDiv (Drug-like library)378.174182.88505
SGTC_1028k015-00294.8 μM0.4339624588309ChemDiv (Drug-like library)351.141023.68304