fluoroquinolonic acid

7-chloro-1-cyclopropyl-6-fluoro-4-oxoquinoline-3-carboxylic acid

Also called cipro. Inhibits gyrases and topoisomerase 1 and 2

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1692
Screen concentration 71.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 483180
SMILES C1CC1N2C=C(C(=O)C3=CC(=C(C=C32)Cl)F)C(=O)O
Standardized SMILES OC(=O)C1=CN(C2CC2)c3cc(Cl)c(F)cc3C1=O
Molecular weight 281.6669
ALogP 2.52
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.62
% growth inhibition (Hom. pool) 2.56


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 483180
Download HIP data (tab-delimited text)  (excel)
Gene:CLP1(YOR250C)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:GCD2(YGR083C)|FD-Score:4.78|P-value:8.55E-7|Clearance:0.91||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:KOG1(YHR186C)|FD-Score:-3.09|P-value:9.87E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:RPF2(YKR081C)|FD-Score:3.87|P-value:5.38E-5|Clearance:0.09||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RSC6(YCR052W)|FD-Score:-3.2|P-value:6.79E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSE1(YML049C)|FD-Score:-3.2|P-value:6.90E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:STS1(YIR011C)|FD-Score:-3.9|P-value:4.78E-5|Clearance:0||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:TAD3(YLR316C)|FD-Score:3.78|P-value:7.76E-5|Clearance:0.59||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TRL1(YJL087C)|FD-Score:3.2|P-value:6.96E-4|Clearance:0.17||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes Gene:CLP1(YOR250C)|FD-Score:-4.26|P-value:1.01E-5|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:GCD2(YGR083C)|FD-Score:4.78|P-value:8.55E-7|Clearance:0.91||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:KOG1(YHR186C)|FD-Score:-3.09|P-value:9.87E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:RPF2(YKR081C)|FD-Score:3.87|P-value:5.38E-5|Clearance:0.09||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RSC6(YCR052W)|FD-Score:-3.2|P-value:6.79E-4|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSE1(YML049C)|FD-Score:-3.2|P-value:6.90E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:STS1(YIR011C)|FD-Score:-3.9|P-value:4.78E-5|Clearance:0||SGD DESC:Protein required for localizing proteasomes to the nucleus; interacts with the karyopherin Srp1p; involved in ubiquitin-mediated protein degradation Gene:TAD3(YLR316C)|FD-Score:3.78|P-value:7.76E-5|Clearance:0.59||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TRL1(YJL087C)|FD-Score:3.2|P-value:6.96E-4|Clearance:0.17||SGD DESC:tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 483180
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ALD2(YMR170C)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:BDF1(YLR399C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BTS1(YPL069C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:COG6(YNL041C)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DMC1(YER179W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DNM1(YLL001W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Gene:GYP1(YOR070C)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HPR1(YDR138W)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IMG1(YCR046C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IRS4(YKR019C)|FD-Score:-4.46|P-value:4.16E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:MFA2(YNL145W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MIG3(YER028C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MRP17(YKL003C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MRPL25(YGR076C)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MTC6(YHR151C)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NRG1(YDR043C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication Gene:OYE3(YPL171C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:RNH201(YNL072W)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RNH203(YLR154C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RNR4(YGR180C)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL7A(YGL076C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RTT109(YLL002W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SNF12(YNR023W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPI1(YER150W)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPO11(YHL022C)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SRB5(YGR104C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRL4(YPL033C)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p Gene:STP3(YLR375W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:YDR290W(YDR290W_d)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YKL115C(YKL115C_d)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL118W(YKL118W_d)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR040C(YLR040C_p)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YOR072W(YOR072W_d)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR139C(YOR139C_d)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL062W(YPL062W_d)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:ADO1(YJR105W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:ALD2(YMR170C)|FD-Score:-3.1|P-value:9.61E-4||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:BDF1(YLR399C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BTS1(YPL069C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:COG6(YNL041C)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DMC1(YER179W)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p Gene:DNM1(YLL001W)|FD-Score:3.44|P-value:2.90E-4||SGD DESC:Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance Gene:GYP1(YOR070C)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HPR1(YDR138W)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IMG1(YCR046C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IRS4(YKR019C)|FD-Score:-4.46|P-value:4.16E-6||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:MFA2(YNL145W)|FD-Score:3.52|P-value:2.14E-4||SGD DESC:Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1 Gene:MIG3(YER028C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MRP17(YKL003C)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MRPL25(YGR076C)|FD-Score:4.5|P-value:3.37E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:MTC6(YHR151C)|FD-Score:4.1|P-value:2.07E-5||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:NRG1(YDR043C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; NRG1 has a paralog, NRG2, that arose from the whole genome duplication Gene:OYE3(YPL171C)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:RNH201(YNL072W)|FD-Score:4.09|P-value:2.19E-5||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RNH203(YLR154C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome Gene:RNR4(YGR180C)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RPL7A(YGL076C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RTT109(YLL002W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SNF12(YNR023W)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPI1(YER150W)|FD-Score:3.93|P-value:4.19E-5||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SPO11(YHL022C)|FD-Score:-3.26|P-value:5.57E-4||SGD DESC:Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation Gene:SRB5(YGR104C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SRL4(YPL033C)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p Gene:STP3(YLR375W)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:YDR290W(YDR290W_d)|FD-Score:-3.4|P-value:3.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YKL115C(YKL115C_d)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1 Gene:YKL118W(YKL118W_d)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR040C(YLR040C_p)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YOR072W(YOR072W_d)|FD-Score:3.89|P-value:5.06E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR139C(YOR139C_d)|FD-Score:-3.14|P-value:8.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YPL062W(YPL062W_d)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGR083C4.788.55E-70.91GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YKR081C3.875.38E-50.09RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YLR316C3.787.76E-50.59TAD3Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs
YJL087C3.206.96E-40.17TRL1tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes
YJL167W3.030.001230.02ERG20Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
YDL098C3.010.001290.01SNU23Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome
YER127W3.000.001350.06LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YFR004W2.940.001643.93E-4RPN11Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
YNL113W2.940.001640.04RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YAL032C2.900.001860.05PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YNL152W2.850.002190.18INN1Essential protein that associates with contractile actomyosin ring; required for ingression of the plasma membrane into the bud neck during cytokinesis; C2 domain, a membrane targeting module, is required for function; activates chitin synthase activity of Chs2p during cytokinesis
YLR076C_d2.670.003800.12YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YOR157C2.550.005350.01PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YLR355C2.540.005560.03ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YJR076C2.510.006020.00CDC11Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR076C4.503.37E-6MRPL25Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan
YLR154C4.261.03E-5RNH203Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS3 that causes Aicardi-Goutieres syndrome
YHR151C4.102.07E-5MTC6Protein of unknown function; mtc6 is synthetically sick with cdc13-1
YNL072W4.092.19E-5RNH201Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
YER150W3.934.19E-5SPI1GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p
YOR072W_d3.895.06E-5YOR072W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
YPL040C3.671.20E-4ISM1Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
YPL171C3.651.31E-4OYE3Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death
YPL062W_d3.601.60E-4YPL062W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation
YER179W3.571.78E-4DMC1Meiosis-specific protein required for repair of double-strand breaks and pairing between homologous chromosomes; homolog of Rad51p and the bacterial RecA protein; binds ssDNA and dsDNA, forms helical filaments; stimulated by Rdh54p
YGL076C3.561.84E-4RPL7ARibosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication
YNL145W3.522.14E-4MFA2Mating pheromone a-factor, made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1
YGR104C3.492.46E-4SRB5Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
YPL069C3.452.80E-4BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YLL001W3.442.90E-4DNM1Dynamin-related GTPase required for mitochondrial fission and morphology; assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis and regulating peroxisome abundance

GO enrichment analysis for SGTC_1692
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0912.51E-12SGTC_31059121040 49.5 μMChembridge (Drug-like library)414489070.0675676
0.0822.26E-10SGTC_1752st044819 83.0 μMTimTec (Natural product derivative library)4412090.0952381
0.0791.43E-9SGTC_392N-ethylmaleimide 8.3 μMMiscellaneous43620.0980392RNA pol III & RNase P/MRP
0.0773.44E-9SGTC_2748diazoxide 86.7 μMMiscellaneous30190.09375
0.0706.09E-8SGTC_13983558-0053 112.0 μMChemDiv (Drug-like library)45323690.125
0.0656.17E-7SGTC_5401187-0821 17.3 μMChemDiv (Drug-like library)8475200.112903
0.0631.31E-6SGTC_917harmaline 728.1 nMTimTec (Natural product library)52809510.0555556ergosterol biosynthesis
0.0631.34E-6SGTC_1676st018489 23.4 μMTimTec (Natural product derivative library)36962350.070422560S ribosome export
0.0621.66E-6SGTC_24875918373 44.0 μMMiscellaneous28762790.157895
0.0621.94E-6SGTC_23509023895 38.9 μMChembridge (Fragment library)29972090.140625ergosterol biosynthesis
0.0587.86E-6SGTC_10064099-6547 255.0 μMChemDiv (Drug-like library)31468330.149254
0.0571.05E-5SGTC_24305571206 200.0 μMMiscellaneous17317710.0649351
0.0571.23E-5SGTC_13903413-2614 145.0 μMChemDiv (Drug-like library)34214320.0461538fatty acid desaturase (OLE1)
0.0571.27E-5SGTC_1833st055984 71.0 μMTimTec (Natural product derivative library)6762880.084507
0.0561.91E-5SGTC_1983454-2873 16.2 μMChemDiv (Drug-like library)7109570.05633860S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_411enrofloxacin100 μM0.61818271188Miscellaneous359.394683-0.71517DNA damage response
SGTC_399oxolinic acid75 μM0.3620694628Miscellaneous261.230141.27316
SGTC_23296138490200 μM0.327273720019Chembridge (Fragment library)231.247222.12914
SGTC_1919st06711352.3 μM0.281691268243TimTec (Natural product derivative library)382.721114.20308TSC3-RPN4
SGTC_2466598340937.09 μM0.2388062879657Miscellaneous345.3448832.90316
SGTC_2470566481721.68 μM0.2388062859561Miscellaneous361.799483.36215
SGTC_294790726397.68 μM0.23611116488146Chembridge (Drug-like library)314.7398432.08415tubulin folding & SWR complex
SGTC_1457k015-003257.3 μM0.2318844302660ChemDiv (Drug-like library)334.6864233.22405
SGTC_1029k015-003064.7 μM0.2258062208316ChemDiv (Drug-like library)289.6888633.32903
SGTC_2001402350257.66 μM0.222222108941Chembridge (Fragment library)195.602441.5402mitochondrial processes