st024011

ethyl 2-[[2-(4-bromophenoxy)acetyl]amino]-3-(1H-indol-3-yl)propanoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1694
Screen concentration 44.9 μM
Source TimTec (Natural product derivative library)
PubChem CID 2787533
SMILES CCOC(=O)C(CC1=CNC2=CC=CC=C21)NC(=O)COC3=CC=C(C=C3)Br
Standardized SMILES CCOC(=O)C(Cc1c[nH]c2ccccc12)NC(=O)COc3ccc(Br)cc3
Molecular weight 445.3064
ALogP 4.01
H-bond donor count 2
H-bond acceptor count 4
Response signature ERG2

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.94
% growth inhibition (Hom. pool) 10.71


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2787533
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:4.17|P-value:1.51E-5|Clearance:0.43||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ERG8(YMR220W)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.06||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FCP1(YMR277W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.03||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:HTS1(YPR033C)|FD-Score:4.5|P-value:3.35E-6|Clearance:0.43||SGD DESC:Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome Gene:IMP4(YNL075W)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.04||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:LCP5(YER127W)|FD-Score:3.51|P-value:2.25E-4|Clearance:0.1||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:MEX67(YPL169C)|FD-Score:5.34|P-value:4.59E-8|Clearance:0.43||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NAM9(YNL137C)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.09||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:PKC1(YBL105C)|FD-Score:4.66|P-value:1.60E-6|Clearance:0.43||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP42(YDR235W)|FD-Score:3.74|P-value:9.16E-5|Clearance:0.06||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:PRP46(YPL151C)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.04||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:RPA43(YOR340C)|FD-Score:3.2|P-value:6.86E-4|Clearance:0.05||SGD DESC:RNA polymerase I subunit A43 Gene:RPB7(YDR404C)|FD-Score:4.41|P-value:5.19E-6|Clearance:0.43||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RRP1(YDR087C)|FD-Score:4.97|P-value:3.31E-7|Clearance:0.43||SGD DESC:Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles Gene:RRP46(YGR095C)|FD-Score:3.23|P-value:6.25E-4|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SEC16(YPL085W)|FD-Score:4.7|P-value:1.30E-6|Clearance:0.43||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SFH1(YLR321C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.31||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:TIF6(YPR016C)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.04||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TOR2(YKL203C)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.07||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:ACC1(YNR016C)|FD-Score:4.17|P-value:1.51E-5|Clearance:0.43||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ERG8(YMR220W)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.06||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FCP1(YMR277W)|FD-Score:3.62|P-value:1.45E-4|Clearance:0.03||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:HTS1(YPR033C)|FD-Score:4.5|P-value:3.35E-6|Clearance:0.43||SGD DESC:Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome Gene:IMP4(YNL075W)|FD-Score:3.41|P-value:3.25E-4|Clearance:0.04||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs Gene:LCP5(YER127W)|FD-Score:3.51|P-value:2.25E-4|Clearance:0.1||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:MEX67(YPL169C)|FD-Score:5.34|P-value:4.59E-8|Clearance:0.43||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NAM9(YNL137C)|FD-Score:3.6|P-value:1.62E-4|Clearance:0.09||SGD DESC:Mitochondrial ribosomal component of the small subunit Gene:PKC1(YBL105C)|FD-Score:4.66|P-value:1.60E-6|Clearance:0.43||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:PRP42(YDR235W)|FD-Score:3.74|P-value:9.16E-5|Clearance:0.06||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:PRP46(YPL151C)|FD-Score:3.37|P-value:3.72E-4|Clearance:0.04||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:RPA43(YOR340C)|FD-Score:3.2|P-value:6.86E-4|Clearance:0.05||SGD DESC:RNA polymerase I subunit A43 Gene:RPB7(YDR404C)|FD-Score:4.41|P-value:5.19E-6|Clearance:0.43||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RRP1(YDR087C)|FD-Score:4.97|P-value:3.31E-7|Clearance:0.43||SGD DESC:Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles Gene:RRP46(YGR095C)|FD-Score:3.23|P-value:6.25E-4|Clearance:0.03||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5) Gene:SEC16(YPL085W)|FD-Score:4.7|P-value:1.30E-6|Clearance:0.43||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SFH1(YLR321C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.31||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:TIF6(YPR016C)|FD-Score:3.33|P-value:4.27E-4|Clearance:0.04||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TOR2(YKL203C)|FD-Score:3.3|P-value:4.85E-4|Clearance:0.07||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2787533
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADK1(YDR226W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ARG8(YOL140W)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:ARG82(YDR173C)|FD-Score:6.55|P-value:2.80E-11||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:BFR1(YOR198C)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BUD3(YCL014W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CSF1(YLR087C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CYB5(YNL111C)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DOA4(YDR069C)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:EEB1(YPL095C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:14.4|P-value:2.36E-47||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG5(YMR015C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV14(YGL054C)|FD-Score:7.41|P-value:6.21E-14||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ETR1(YBR026C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:GAL11(YOL051W)|FD-Score:12.9|P-value:1.55E-38||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GLE2(YER107C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HFI1(YPL254W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HOS2(YGL194C)|FD-Score:7.76|P-value:4.39E-15||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HPR1(YDR138W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HSL7(YBR133C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IRC4(YDR540C)|FD-Score:4.42|P-value:4.86E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LDB19(YOR322C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LGE1(YPL055C)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIP2(YLR239C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LPD1(YFL018C)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:LRE1(YCL051W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LSM6(YDR378C)|FD-Score:4.86|P-value:5.99E-7||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LSM7(YNL147W)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MOD5(YOR274W)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRC1(YCL061C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:NAT1(YDL040C)|FD-Score:5.2|P-value:1.02E-7||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NMA111(YNL123W)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NOT3(YIL038C)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPR3(YHL023C)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:NUM1(YDR150W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:NUP170(YBL079W)|FD-Score:4.85|P-value:6.02E-7||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PDR1(YGL013C)|FD-Score:15.4|P-value:4.12E-54||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR16(YNL231C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PGD1(YGL025C)|FD-Score:14.1|P-value:2.17E-45||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PRS3(YHL011C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTR2(YKR093W)|FD-Score:4.37|P-value:6.20E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAV1(YJR033C)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RAV2(YDR202C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:REG1(YDR028C)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPN4(YDL020C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC3(YDR159W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SBH1(YER087C-B)|FD-Score:5.11|P-value:1.62E-7||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SIF2(YBR103W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SKY1(YMR216C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLX9(YGR081C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNF2(YOR290C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOH1(YGL127C)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPT23(YKL020C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SPT3(YDR392W)|FD-Score:12|P-value:1.40E-33||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT8(YLR055C)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRB2(YHR041C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRL3(YKR091W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SWC5(YBR231C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:THO2(YNL139C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:THP1(YOL072W)|FD-Score:5.28|P-value:6.41E-8||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:VHC1(YBR235W_p)|FD-Score:4.34|P-value:7.16E-6||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:YAL067W-A(YAL067W-A_p)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR072C-A(YBR072C-A_p)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCL012C(YCL012C)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YDR379C-A(YDR379C-A)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YFL041W-A(YFL041W-A_p)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Putative protein of unknown function Gene:YGR121W-A(YGR121W-A_p)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Putative protein of unknown function Gene:YGR122W(YGR122W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YIL002W-A(YIL002W-A_p)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL049W(YJL049W_p)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Putative protein of unknown function; YJL049W is a non-essential gene Gene:YKL077W(YKL077W_p)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YML007C-A(YML007C-A_p)|FD-Score:6.04|P-value:7.63E-10||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML122C(YML122C_d)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Putative protein of unknown function Gene:YNL235C(YNL235C_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL050C(YOL050C_d)|FD-Score:15.9|P-value:4.40E-57||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPK1(YKL126W)|FD-Score:15.1|P-value:5.64E-52||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YRR1(YOR162C)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ACB1(YGR037C)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADK1(YDR226W)|FD-Score:3.99|P-value:3.31E-5||SGD DESC:Adenylate kinase, required for purine metabolism; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress Gene:ARG8(YOL140W)|FD-Score:-3.22|P-value:6.43E-4||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:ARG82(YDR173C)|FD-Score:6.55|P-value:2.80E-11||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:BFR1(YOR198C)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BUD3(YCL014W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding Gene:CSF1(YLR087C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CYB5(YNL111C)|FD-Score:3.89|P-value:4.95E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DOA4(YDR069C)|FD-Score:3.36|P-value:3.86E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:EEB1(YPL095C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ERG2(YMR202W)|FD-Score:14.4|P-value:2.36E-47||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG5(YMR015C)|FD-Score:3.45|P-value:2.76E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERV14(YGL054C)|FD-Score:7.41|P-value:6.21E-14||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:ETR1(YBR026C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:GAL11(YOL051W)|FD-Score:12.9|P-value:1.55E-38||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GLE2(YER107C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HFI1(YPL254W)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HOS2(YGL194C)|FD-Score:7.76|P-value:4.39E-15||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HPR1(YDR138W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HSL7(YBR133C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:IRC4(YDR540C)|FD-Score:4.42|P-value:4.86E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LDB19(YOR322C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Protein involved in regulating the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane; null mutant has reduced affinity for alcian blue dye Gene:LGE1(YPL055C)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LIP2(YLR239C)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:LPD1(YFL018C)|FD-Score:3.88|P-value:5.21E-5||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:LRE1(YCL051W)|FD-Score:3.19|P-value:7.01E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LSM6(YDR378C)|FD-Score:4.86|P-value:5.99E-7||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:LSM7(YNL147W)|FD-Score:4.52|P-value:3.10E-6||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MOD5(YOR274W)|FD-Score:3.88|P-value:5.24E-5||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRC1(YCL061C)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:S-phase checkpoint protein required for DNA replication; interacts with and stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; protects uncapped telomeres Gene:NAT1(YDL040C)|FD-Score:5.2|P-value:1.02E-7||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NMA111(YNL123W)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NOT3(YIL038C)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPR3(YHL023C)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair Gene:NUM1(YDR150W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:NUP170(YBL079W)|FD-Score:4.85|P-value:6.02E-7||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:PDR1(YGL013C)|FD-Score:15.4|P-value:4.12E-54||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR16(YNL231C)|FD-Score:4.12|P-value:1.86E-5||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PGD1(YGL025C)|FD-Score:14.1|P-value:2.17E-45||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PRS3(YHL011C)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTR2(YKR093W)|FD-Score:4.37|P-value:6.20E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:RAV1(YJR033C)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RAV2(YDR202C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:REG1(YDR028C)|FD-Score:4.41|P-value:5.24E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RPN4(YDL020C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC3(YDR159W)|FD-Score:4.21|P-value:1.28E-5||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SBH1(YER087C-B)|FD-Score:5.11|P-value:1.62E-7||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SIF2(YBR103W)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus Gene:SKY1(YMR216C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SLX9(YGR081C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNF2(YOR290C)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOH1(YGL127C)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SPT23(YKL020C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:SPT3(YDR392W)|FD-Score:12|P-value:1.40E-33||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SPT8(YLR055C)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRB2(YHR041C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRL3(YKR091W)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:SWC5(YBR231C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:THO2(YNL139C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:THP1(YOL072W)|FD-Score:5.28|P-value:6.41E-8||SGD DESC:Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding Gene:VHC1(YBR235W_p)|FD-Score:4.34|P-value:7.16E-6||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:YAL067W-A(YAL067W-A_p)|FD-Score:5.17|P-value:1.20E-7||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR072C-A(YBR072C-A_p)|FD-Score:3.95|P-value:3.94E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCL012C(YCL012C)|FD-Score:-3.3|P-value:4.75E-4||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YDR379C-A(YDR379C-A)|FD-Score:3.93|P-value:4.24E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YFL041W-A(YFL041W-A_p)|FD-Score:4.62|P-value:1.88E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.05|P-value:2.58E-5||SGD DESC:Putative protein of unknown function Gene:YGR121W-A(YGR121W-A_p)|FD-Score:3.79|P-value:7.58E-5||SGD DESC:Putative protein of unknown function Gene:YGR122W(YGR122W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress Gene:YIL002W-A(YIL002W-A_p)|FD-Score:3.26|P-value:5.49E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL049W(YJL049W_p)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Putative protein of unknown function; YJL049W is a non-essential gene Gene:YKL077W(YKL077W_p)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YML007C-A(YML007C-A_p)|FD-Score:6.04|P-value:7.63E-10||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YML122C(YML122C_d)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR105W-A(YMR105W-A_p)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Putative protein of unknown function Gene:YNL235C(YNL235C_d)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex Gene:YOL050C(YOL050C_d)|FD-Score:15.9|P-value:4.40E-57||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:YPK1(YKL126W)|FD-Score:15.1|P-value:5.64E-52||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YRR1(YOR162C)|FD-Score:4.64|P-value:1.70E-6||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPL169C5.344.59E-80.43MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YDR087C4.973.31E-70.43RRP1Essential evolutionarily conserved nucleolar protein necessary for biogenesis of 60S ribosomal subunits and processing of pre-rRNAs to mature rRNAs, associated with several distinct 66S pre-ribosomal particles
YPL085W4.701.30E-60.43SEC16COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
YBL105C4.661.60E-60.43PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YPR033C4.503.35E-60.43HTS1Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
YDR404C4.415.19E-60.43RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YNR016C4.171.51E-50.43ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YDR235W3.749.16E-50.06PRP42U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
YMR220W3.681.17E-40.06ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YMR277W3.621.45E-40.03FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YNL137C3.601.62E-40.09NAM9Mitochondrial ribosomal component of the small subunit
YER127W3.512.25E-40.10LCP5Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
YNL075W3.413.25E-40.04IMP4Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
YPL151C3.373.72E-40.04PRP46Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
YPR016C3.334.27E-40.04TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL050C_d15.904.40E-57YOL050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
YGL013C15.404.12E-54PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YKL126W15.105.64E-52YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YMR202W14.402.36E-47ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YGL025C14.102.17E-45PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YOL051W12.901.55E-38GAL11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
YDR392W12.001.40E-33SPT3Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
YGL194C7.764.39E-15HOS2Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS
YGL054C7.416.21E-14ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YDR173C6.552.80E-11ARG82Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes
YML007C-A_p6.047.63E-10YML007C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
YPL055C5.591.16E-8LGE1Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division
YML122C_d5.581.22E-8YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YOL072W5.286.41E-8THP1Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding
YDL040C5.201.02E-7NAT1Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing

GO enrichment analysis for SGTC_1694
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4413.35E-279SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.0697674sphingolipid biosynthesis & PDR1
0.4232.72E-255SGTC_1687st020778 55.4 μMTimTec (Natural product derivative library)42592990.119565
0.3889.16E-211SGTC_12870971-0001 34.6 μMChemDiv (Drug-like library)46658690.0875sphingolipid biosynthesis & PDR1
0.3841.79E-206SGTC_5983937-0236 20.2 μMChemDiv (Drug-like library)13793130.0705882ERG2
0.3455.69E-165SGTC_2752diclazuril 33.1 μMMiscellaneous4563890.0543478
0.3421.22E-161SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.0705882
0.3392.49E-158SGTC_1826st055655 41.6 μMTimTec (Natural product derivative library)6761680.347222ERG2
0.3373.57E-157SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.0697674PDR1
0.3362.78E-155SGTC_1700st030840 7.9 μMTimTec (Natural product derivative library)54993070.145833PDR1
0.3351.18E-154SGTC_1877wortmannin 25.0 μMICCB bioactive library3121450.0747664
0.3263.09E-146SGTC_8410251-0215 91.2 μMChemDiv (Drug-like library)38886140.0588235
0.3151.66E-136SGTC_234nsc-17383 10.7 μMMiscellaneous4089740.101124ERG2
0.3102.07E-131SGTC_9551216-0110 33.0 μMChemDiv (Drug-like library)36197190.151899sphingolipid biosynthesis & PDR1
0.3092.68E-131SGTC_28589025781 9.0 μMChembridge (Drug-like library)48949800.0843373sphingolipid biosynthesis & PDR1
0.3096.58E-131SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)28435220.121951ERG2

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1931st07443857.1 μM0.6031752787313TimTec (Natural product derivative library)350.410983.91323
SGTC_2017513292760.65 μM0.3833338367Chembridge (Fragment library)188.268842.45121
SGTC_1893st05935336.1 μM0.3827163112488TimTec (Natural product derivative library)553.391535.63433
SGTC_1998st07579776.8 μM0.38235324208315TimTec (Natural product derivative library)260.33153.40722mitochondrial response to ROS
SGTC_8251988-222489.6 μM0.3698631180859ChemDiv (Drug-like library)399.25962.86316cell wall signaling
SGTC_1826st05565541.6 μM0.347222676168TimTec (Natural product derivative library)329.395063.2332ERG2
SGTC_7950685-026578 μM0.34375302754ChemDiv (Drug-like library)340.599643.86212excess fatty acid
SGTC_1790st04571143.5 μM0.333333585119TimTec (Natural product derivative library)459.3848795.01539
SGTC_1756st04155835.5 μM0.329673955792TimTec (Natural product derivative library)564.649284.77928
SGTC_452diindolylmethane8 μM0.3214293071ICCB bioactive library246.306464.39120RNA processing & uracil transport