st025591

4-amino-2-[(2-nitrophenyl)sulfanylamino]-4-oxobutanoic acid

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1696
Screen concentration 34.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 2787147
SMILES C1=CC=C(C(=C1)[N+](=O)[O-])SNC(CC(=O)N)C(=O)O
Standardized SMILES NC(=O)CC(NSc1ccccc1[N+](=O)[O-])C(=O)O
Molecular weight 285.2764
ALogP 0.49
H-bond donor count 3
H-bond acceptor count 7
Response signature SWF1 & branched chain AA biosynthesis

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.73
% growth inhibition (Hom. pool) 6.95


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2787147
Download HIP data (tab-delimited text)  (excel)
Gene:DML1(YMR211W)|FD-Score:4.44|P-value:4.48E-6|Clearance:0.8||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:GCD2(YGR083C)|FD-Score:3.12|P-value:9.07E-4|Clearance:0.04||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:LCP5(YER127W)|FD-Score:-3.1|P-value:9.58E-4|Clearance:0||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:LSM2(YBL026W)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.04||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MEX67(YPL169C)|FD-Score:-3.26|P-value:5.49E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:RPB8(YOR224C)|FD-Score:4.27|P-value:9.63E-6|Clearance:0.8||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL32(YBL092W)|FD-Score:5.05|P-value:2.23E-7|Clearance:0.8||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RSC58(YLR033W)|FD-Score:5.36|P-value:4.07E-8|Clearance:0.8||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SPT6(YGR116W)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SSS1(YDR086C)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.06||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:STH1(YIL126W)|FD-Score:3.47|P-value:2.58E-4|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUP35(YDR172W)|FD-Score:3.31|P-value:4.65E-4|Clearance:0.09||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TFB4(YPR056W)|FD-Score:-3.33|P-value:4.32E-4|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:YDL163W(YDL163W_d)|FD-Score:-3.22|P-value:6.51E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YLR379W(YLR379W_d)|FD-Score:3.47|P-value:2.61E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C Gene:YTH1(YPR107C)|FD-Score:6.16|P-value:3.54E-10|Clearance:0.8||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:DML1(YMR211W)|FD-Score:4.44|P-value:4.48E-6|Clearance:0.8||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:GCD2(YGR083C)|FD-Score:3.12|P-value:9.07E-4|Clearance:0.04||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:LCP5(YER127W)|FD-Score:-3.1|P-value:9.58E-4|Clearance:0||SGD DESC:Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus Gene:LSM2(YBL026W)|FD-Score:3.16|P-value:7.85E-4|Clearance:0.04||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MEX67(YPL169C)|FD-Score:-3.26|P-value:5.49E-4|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:RPB8(YOR224C)|FD-Score:4.27|P-value:9.63E-6|Clearance:0.8||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL32(YBL092W)|FD-Score:5.05|P-value:2.23E-7|Clearance:0.8||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RSC58(YLR033W)|FD-Score:5.36|P-value:4.07E-8|Clearance:0.8||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SPT6(YGR116W)|FD-Score:-3.11|P-value:9.41E-4|Clearance:0||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SSS1(YDR086C)|FD-Score:3.22|P-value:6.31E-4|Clearance:0.06||SGD DESC:Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p Gene:STH1(YIL126W)|FD-Score:3.47|P-value:2.58E-4|Clearance:0||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUP35(YDR172W)|FD-Score:3.31|P-value:4.65E-4|Clearance:0.09||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TFB4(YPR056W)|FD-Score:-3.33|P-value:4.32E-4|Clearance:0||SGD DESC:Subunit of TFIIH complex, involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p Gene:YDL163W(YDL163W_d)|FD-Score:-3.22|P-value:6.51E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase Gene:YLR379W(YLR379W_d)|FD-Score:3.47|P-value:2.61E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C Gene:YTH1(YPR107C)|FD-Score:6.16|P-value:3.54E-10|Clearance:0.8||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2787147
Download HOP data (tab-delimited text)  (excel)
Gene:ARO7(YPR060C)|FD-Score:4.5|P-value:3.38E-6||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:CEM1(YER061C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CMC1(YKL137W)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:COQ5(YML110C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CRS5(YOR031W)|FD-Score:3.76|P-value:8.60E-5||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DAL81(YIR023W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIA1(YMR316W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:EDS1(YBR033W_p)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EXO5(YBR163W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FAR7(YFR008W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:GEM1(YAL048C)|FD-Score:5.22|P-value:8.98E-8||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:GEP4(YHR100C)|FD-Score:4.87|P-value:5.54E-7||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIT1(YCR098C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GLY1(YEL046C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GSP2(YOR185C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:GTR2(YGR163W)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HOM6(YJR139C)|FD-Score:12.5|P-value:4.47E-36||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HTD2(YHR067W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:ILM1(YJR118C)|FD-Score:4.85|P-value:6.10E-7||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:ILV1(YER086W)|FD-Score:6.94|P-value:1.92E-12||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:ILV6(YCL009C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IMH1(YLR309C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Gene:IRS4(YKR019C)|FD-Score:-5.59|P-value:1.12E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LIP5(YOR196C)|FD-Score:6.34|P-value:1.14E-10||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LPD1(YFL018C)|FD-Score:6.99|P-value:1.35E-12||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:LTE1(YAL024C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:7.35|P-value:9.91E-14||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDM12(YOL009C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MEI4(YER044C-A)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MEP2(YNL142W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET10(YFR030W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MET17(YLR303W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MGR2(YPL098C)|FD-Score:5.29|P-value:6.28E-8||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MUP3(YHL036W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NST1(YNL091W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:OPI3(YJR073C)|FD-Score:7.52|P-value:2.71E-14||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAF1(YBR279W)|FD-Score:-4.54|P-value:2.79E-6||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PCL5(YHR071W)|FD-Score:-3.78|P-value:7.75E-5||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PIR3(YKL163W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PMT3(YOR321W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:RAV2(YDR202C)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RMT2(YDR465C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPN4(YDL020C)|FD-Score:7.93|P-value:1.10E-15||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRG9(YNL213C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SDH1(YKL148C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SEC66(YBR171W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIT4(YDL047W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SPF1(YEL031W)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SRC1(YML034W)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SWF1(YDR126W)|FD-Score:24|P-value:2.19E-127||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:THR1(YHR025W)|FD-Score:8.76|P-value:9.82E-19||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TOS2(YGR221C)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TPN1(YGL186C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSC3(YBR058C-A)|FD-Score:8.83|P-value:5.18E-19||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUS1(YLR425W)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBA3(YPR066W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UME6(YDR207C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:URA10(YMR271C)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAM3(YOR106W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VBA5(YKR105C_p)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VPS27(YNR006W)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YDR340W(YDR340W_d)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YJR038C(YJR038C_d)|FD-Score:-3.81|P-value:6.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR005C(YKR005C_p)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Putative protein of unknown function Gene:YLR063W(YLR063W_p)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YLR173W(YLR173W_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function Gene:YLR345W(YLR345W_p)|FD-Score:3.73|P-value:9.70E-5||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YLR366W(YLR366W_d)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML034C-A(YML034C-A_d)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL143C(YNL143C_p)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR200W(YOR200W_d)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPK1(YKL126W)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YVH1(YIR026C)|FD-Score:4.27|P-value:9.92E-6||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ARO7(YPR060C)|FD-Score:4.5|P-value:3.38E-6||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:CEM1(YER061C)|FD-Score:4.04|P-value:2.69E-5||SGD DESC:Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration Gene:CMC1(YKL137W)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:COQ5(YML110C)|FD-Score:3.26|P-value:5.57E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CRS5(YOR031W)|FD-Score:3.76|P-value:8.60E-5||SGD DESC:Copper-binding metallothionein, required for wild-type copper resistance Gene:DAL81(YIR023W)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DIA1(YMR316W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:EDS1(YBR033W_p)|FD-Score:4.07|P-value:2.32E-5||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EXO5(YBR163W)|FD-Score:4.69|P-value:1.38E-6||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FAR7(YFR008W)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress Gene:GEM1(YAL048C)|FD-Score:5.22|P-value:8.98E-8||SGD DESC:Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases Gene:GEP4(YHR100C)|FD-Score:4.87|P-value:5.54E-7||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIT1(YCR098C)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GLY1(YEL046C)|FD-Score:3.53|P-value:2.10E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GSP2(YOR185C)|FD-Score:3.19|P-value:7.10E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:GTR2(YGR163W)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HOM6(YJR139C)|FD-Score:12.5|P-value:4.47E-36||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HTD2(YHR067W)|FD-Score:3.17|P-value:7.68E-4||SGD DESC:Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology Gene:ILM1(YJR118C)|FD-Score:4.85|P-value:6.10E-7||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:ILV1(YER086W)|FD-Score:6.94|P-value:1.92E-12||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:ILV6(YCL009C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Regulatory subunit of acetolactate synthase, which catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria Gene:IMH1(YLR309C)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi Gene:IRS4(YKR019C)|FD-Score:-5.59|P-value:1.12E-8||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LIP5(YOR196C)|FD-Score:6.34|P-value:1.14E-10||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:LPD1(YFL018C)|FD-Score:6.99|P-value:1.35E-12||SGD DESC:Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes Gene:LTE1(YAL024C)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:7.35|P-value:9.91E-14||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MDM12(YOL009C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MEI4(YER044C-A)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MEP2(YNL142W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Ammonium permease involved in regulation of pseudohyphal growth; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation Gene:MET10(YFR030W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Subunit alpha of assimilatory sulfite reductase, which converts sulfite into sulfide Gene:MET17(YLR303W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis Gene:MGR2(YPL098C)|FD-Score:5.29|P-value:6.28E-8||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MUP3(YHL036W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NST1(YNL091W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:OPI3(YJR073C)|FD-Score:7.52|P-value:2.71E-14||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAF1(YBR279W)|FD-Score:-4.54|P-value:2.79E-6||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PCL5(YHR071W)|FD-Score:-3.78|P-value:7.75E-5||SGD DESC:Cyclin, interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity Gene:PIR3(YKL163W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; PIR3 has a paralog, HSP150, that arose from the whole genome duplication Gene:PMT3(YOR321W)|FD-Score:-3.14|P-value:8.38E-4||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:RAV2(YDR202C)|FD-Score:-3.26|P-value:5.65E-4||SGD DESC:Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme Gene:RMT2(YDR465C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress Gene:RPN4(YDL020C)|FD-Score:7.93|P-value:1.10E-15||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RRG9(YNL213C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SDH1(YKL148C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits Gene:SEC66(YBR171W)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SIT4(YDL047W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SPF1(YEL031W)|FD-Score:5.16|P-value:1.24E-7||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SRC1(YML034W)|FD-Score:4.04|P-value:2.68E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SWF1(YDR126W)|FD-Score:24|P-value:2.19E-127||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:THR1(YHR025W)|FD-Score:8.76|P-value:9.82E-19||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TOS2(YGR221C)|FD-Score:3.1|P-value:9.79E-4||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TPN1(YGL186C)|FD-Score:3.74|P-value:9.29E-5||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TSC3(YBR058C-A)|FD-Score:8.83|P-value:5.18E-19||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUS1(YLR425W)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:UBA3(YPR066W)|FD-Score:3.14|P-value:8.42E-4||SGD DESC:Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UME6(YDR207C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p Gene:URA10(YMR271C)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:URE2(YNL229C)|FD-Score:3.38|P-value:3.62E-4||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VAM3(YOR106W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VBA5(YKR105C_p)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; similar to Vba3p Gene:VPS27(YNR006W)|FD-Score:-3.26|P-value:5.64E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YDR340W(YDR340W_d)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative protein of unknown function Gene:YGL007C-A(YGL007C-A_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function, identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding Gene:YJR038C(YJR038C_d)|FD-Score:-3.81|P-value:6.99E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR005C(YKR005C_p)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Putative protein of unknown function Gene:YLR063W(YLR063W_p)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YLR173W(YLR173W_p)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Putative protein of unknown function Gene:YLR345W(YLR345W_p)|FD-Score:3.73|P-value:9.70E-5||SGD DESC:Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene Gene:YLR366W(YLR366W_d)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A Gene:YML034C-A(YML034C-A_d)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL143C(YNL143C_p)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR200W(YOR200W_d)|FD-Score:3.85|P-value:5.94E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPK1(YKL126W)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YVH1(YIR026C)|FD-Score:4.27|P-value:9.92E-6||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR107C6.163.54E-100.80YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YLR033W5.364.07E-80.80RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YBL092W5.052.23E-70.80RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
YMR211W4.444.48E-60.80DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YOR224C4.279.63E-60.80RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YIL126W3.472.58E-40.00STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YLR379W_d3.472.61E-40.16YLR379W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C
YDR172W3.314.65E-40.09SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YDR086C3.226.31E-40.06SSS1Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
YBL026W3.167.85E-40.04LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YGR083C3.129.07E-40.04GCD2Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YKL004W3.080.001040.05AUR1Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
YNL126W3.030.001230.06SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YIL026C2.970.001480.03IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YOR217W2.940.001660.00RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR126W24.002.19E-127SWF1Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
YJR139C12.504.47E-36HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YBR058C-A8.835.18E-19TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YHR025W8.769.82E-19THR1Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
YDL020C7.931.10E-15RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YJR073C7.522.71E-14OPI3Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
YMR021C7.359.91E-14MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YFL018C6.991.35E-12LPD1Dihydrolipoamide dehydrogenase, the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes
YER086W6.941.92E-12ILV1Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
YOR196C6.341.14E-10LIP5Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase
YPL098C5.296.28E-8MGR2Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype)
YAL048C5.228.98E-8GEM1Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases
YEL031W5.161.24E-7SPF1P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
YKL126W5.141.39E-7YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR425W4.943.89E-7TUS1Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate

GO enrichment analysis for SGTC_1696
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4071.33E-234SGTC_9841492-0255 2.9 μMChemDiv (Drug-like library)15851980.104478SWF1 & branched chain AA biosynthesis
0.3975.12E-222SGTC_1070358-0022 2.7 μMChemDiv (Drug-like library)5211060.127273SWF1 & branched chain AA biosynthesis
0.3693.24E-190SGTC_340469-0683 81.8 μMChemDiv (Drug-like library)36909230.1875SWF1 & branched chain AA biosynthesis
0.3122.45E-133SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.115385
0.2966.39E-120SGTC_7961234-0020 70.7 μMChemDiv (Drug-like library)464950340.0810811SWF1 & branched chain AA biosynthesis
0.2955.28E-119SGTC_20435101342 149.0 μMChembridge (Fragment library)6784510.0588235SWF1 & branched chain AA biosynthesis
0.2822.78E-108SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0441176TSC3-RPN4
0.2796.75E-106SGTC_8420271-0008 11.1 μMChemDiv (Drug-like library)12217090.0983607heme biosynthesis & mitochondrial translocase
0.2743.03E-102SGTC_900870-0863 4.6 μMChemDiv (Drug-like library)17381640.126761
0.2703.10E-99SGTC_21325317594 122.7 μMChembridge (Fragment library)7945770.0704225SWF1 & branched chain AA biosynthesis
0.2621.35E-93SGTC_21125488546 200.0 μMChembridge (Fragment library)6007570.03125TSC3-RPN4
0.2527.42E-86SGTC_20345197015 53.1 μMChembridge (Fragment library)7806760.151515SWF1 & branched chain AA biosynthesis
0.2439.89E-80SGTC_460329-0187a 2.2 μMChemDiv (Drug-like library)464948170.123077
0.2294.80E-71SGTC_11951502-0147 41.1 μMChemDiv (Drug-like library)8179400.0882353
0.2195.80E-65SGTC_20775212015 200.0 μMChembridge (Fragment library)7809000.09375

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3051682-259520.8 μM0.411765226033ChemDiv (Drug-like library)260.311584.35114RNA processing & uracil transport
SGTC_1764st04450293.5 μM0.38407TimTec (Natural product derivative library)214.223.27413
SGTC_9262268-140159.4 μM0.2968752931578ChemDiv (Drug-like library)390.388724.27926TSC3-RPN4
SGTC_797k781-0471270 μM0.26984123738129ChemDiv (Drug-like library)306.701143.4125RPP1 & pyrimidine depletion
SGTC_8470342-02745.54 μM0.26923118097ChemDiv (Drug-like library)218.165641.28204
SGTC_850868-0259160.06 μM0.26315883173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_458nppb133 μM0.2615384549ICCB bioactive library300.309243.52325
SGTC_230213-001140 μM0.259259627608ChemDiv (Drug-like library)304.215123.06317copper-dependent oxidative stress
SGTC_260302-0163425.16 μM0.2586213100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase
SGTC_12941000-1494117 μM0.258065712797ChemDiv (Drug-like library)281.266143.25604