st025554

(5Z)-5-(1,3-benzodioxol-5-ylmethylidene)-1,3-thiazolidine-2,4-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1697
Screen concentration 27.7 μM
Source TimTec (Natural product derivative library)
PubChem CID 1269519
SMILES C1OC2=C(O1)C=C(C=C2)C=C3C(=O)NC(=O)S3
Standardized SMILES O=C1NC(=O)C(=Cc2ccc3OCOc3c2)S1
Molecular weight 249.2426
ALogP 1.71
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.66
% growth inhibition (Hom. pool) 5.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1269519
Download HIP data (tab-delimited text)  (excel)
Gene:ISD11(YER048W-A)|FD-Score:-3.21|P-value:6.68E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:MCM5(YLR274W)|FD-Score:3.74|P-value:9.17E-5|Clearance:0.38||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:PWP2(YCR057C)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.38||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:QNS1(YHR074W)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.15||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:ROK1(YGL171W)|FD-Score:3.74|P-value:9.14E-5|Clearance:0.38||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPP1(YHR062C)|FD-Score:4.13|P-value:1.78E-5|Clearance:0.38||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP4(YHR069C)|FD-Score:3.85|P-value:5.89E-5|Clearance:0.38||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC6(YCR052W)|FD-Score:-4.09|P-value:2.13E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SNM1(YDR478W)|FD-Score:3.13|P-value:8.75E-4|Clearance:0.07||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:YDL152W(YDL152W_d)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:ISD11(YER048W-A)|FD-Score:-3.21|P-value:6.68E-4|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:MCM5(YLR274W)|FD-Score:3.74|P-value:9.17E-5|Clearance:0.38||SGD DESC:Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase Gene:PWP2(YCR057C)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.38||SGD DESC:Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis Gene:QNS1(YHR074W)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.15||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:ROK1(YGL171W)|FD-Score:3.74|P-value:9.14E-5|Clearance:0.38||SGD DESC:RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation Gene:RPP1(YHR062C)|FD-Score:4.13|P-value:1.78E-5|Clearance:0.38||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP4(YHR069C)|FD-Score:3.85|P-value:5.89E-5|Clearance:0.38||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RSC6(YCR052W)|FD-Score:-4.09|P-value:2.13E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SNM1(YDR478W)|FD-Score:3.13|P-value:8.75E-4|Clearance:0.07||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:YDL152W(YDL152W_d)|FD-Score:-3.63|P-value:1.40E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1269519
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:4.83|P-value:6.67E-7||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AIM11(YER093C-A)|FD-Score:4.4|P-value:5.52E-6||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:APE2(YKL157W)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:6.85|P-value:3.68E-12||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:7.41|P-value:6.32E-14||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BRP1(YGL007W_d)|FD-Score:6.05|P-value:7.34E-10||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CKB2(YOR039W)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLC1(YGR167W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:DDI3(YNL335W_p)|FD-Score:7.1|P-value:6.23E-13||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:ELP3(YPL086C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ENV10(YLR065C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:GCN2(YDR283C)|FD-Score:9.64|P-value:2.60E-22||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:12.5|P-value:6.61E-36||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HIM1(YDR317W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:ISF1(YMR081C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:ISM1(YPL040C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:MGA2(YIR033W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MMT2(YPL224C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MRN1(YPL184C)|FD-Score:6.23|P-value:2.40E-10||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:NDT80(YHR124W)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NGR1(YBR212W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NTH2(YBR001C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OM45(YIL136W)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:OPT2(YPR194C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PMT3(YOR321W)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RPI1(YIL119C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RRI1(YDL216C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SBH1(YER087C-B)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SEH1(YGL100W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SKI2(YLR398C)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SSD1(YDR293C)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SVS1(YPL163C)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:11.2|P-value:1.59E-29||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:11.1|P-value:8.90E-29||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.37|P-value:2.80E-17||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.82|P-value:4.54E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:7.63|P-value:1.15E-14||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UIP4(YPL186C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VPS17(YOR132W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YDL172C(YDL172C_d)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:14.3|P-value:6.91E-47||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:4.99|P-value:3.08E-7||SGD DESC:Putative protein of unknown function Gene:YHR125W(YHR125W_d)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR210C(YHR210C_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YJR038C(YJR038C_d)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR287C(YLR287C_p)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YNR064C(YNR064C)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOR139C(YOR139C_d)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YOR228C(YOR228C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YUR1(YJL139C)|FD-Score:4.31|P-value:8.30E-6||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:ACB1(YGR037C)|FD-Score:4.83|P-value:6.67E-7||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AIM11(YER093C-A)|FD-Score:4.4|P-value:5.52E-6||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:APE2(YKL157W)|FD-Score:5.07|P-value:1.97E-7||SGD DESC:Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication Gene:ARO1(YDR127W)|FD-Score:6.85|P-value:3.68E-12||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:7.41|P-value:6.32E-14||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:BRP1(YGL007W_d)|FD-Score:6.05|P-value:7.34E-10||SGD DESC:Dubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1 Gene:CKB2(YOR039W)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:CLC1(YGR167W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:DDI3(YNL335W_p)|FD-Score:7.1|P-value:6.23E-13||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:ELP3(YPL086C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin Gene:ENV10(YLR065C)|FD-Score:-3.29|P-value:4.97E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:GCN2(YDR283C)|FD-Score:9.64|P-value:2.60E-22||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:4.22|P-value:1.21E-5||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:12.5|P-value:6.61E-36||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HIM1(YDR317W)|FD-Score:3.58|P-value:1.73E-4||SGD DESC:Protein of unknown function involved in DNA repair Gene:ISF1(YMR081C)|FD-Score:-3.22|P-value:6.50E-4||SGD DESC:Serine-rich, hydrophilic protein with similarity to Mbr1p; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant Gene:ISM1(YPL040C)|FD-Score:-3.25|P-value:5.72E-4||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:MGA2(YIR033W)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MMT2(YPL224C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MRN1(YPL184C)|FD-Score:6.23|P-value:2.40E-10||SGD DESC:RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation Gene:NDT80(YHR124W)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Meiosis-specific transcription factor required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) Gene:NGR1(YBR212W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NTH2(YBR001C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OM45(YIL136W)|FD-Score:3.29|P-value:5.04E-4||SGD DESC:Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress Gene:OPT2(YPR194C)|FD-Score:3.39|P-value:3.50E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PMT3(YOR321W)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RPI1(YIL119C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RRI1(YDL216C)|FD-Score:3.43|P-value:3.04E-4||SGD DESC:Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling Gene:SBH1(YER087C-B)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SEH1(YGL100W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SKI2(YLR398C)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay Gene:SSD1(YDR293C)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SVS1(YPL163C)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:11.2|P-value:1.59E-29||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:11.1|P-value:8.90E-29||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.37|P-value:2.80E-17||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:6.82|P-value:4.54E-12||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:7.63|P-value:1.15E-14||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UIP4(YPL186C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Protein that interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope Gene:VPS17(YOR132W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes Gene:YDL172C(YDL172C_d)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:14.3|P-value:6.91E-47||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGL117W(YGL117W_p)|FD-Score:4.99|P-value:3.08E-7||SGD DESC:Putative protein of unknown function Gene:YHR125W(YHR125W_d)|FD-Score:5.18|P-value:1.12E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR210C(YHR210C_p)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions Gene:YJR038C(YJR038C_d)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR287C(YLR287C_p)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YNR064C(YNR064C)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOR139C(YOR139C_d)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W Gene:YOR228C(YOR228C)|FD-Score:3.31|P-value:4.60E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:YUR1(YJL139C)|FD-Score:4.31|P-value:8.30E-6||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C4.131.78E-50.38RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YHR069C3.855.89E-50.38RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YGL171W3.749.14E-50.38ROK1RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
YLR274W3.749.17E-50.38MCM5Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
YCR057C3.651.30E-40.38PWP2Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
YHR074W3.285.26E-40.15QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YDR478W3.138.75E-40.07SNM1Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
YER146W3.060.001110.04LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YLR272C3.020.001270.15YCS4Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
YBR160W2.870.002040.00CDC28Catalytic subunit of the main cell cycle cyclin-dependent kinase (CDK); alternately associates with G1 cyclins (CLNs) and G2/M cyclins (CLBs) which direct the CDK to specific substrates; involved in modulating membrane trafficking dynamics; protein abundance increases in response to DNA replication stress
YER008C2.870.002070.02SEC3Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
YIL048W2.850.002200.02NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YPR016C2.830.002320.06TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YER012W2.770.002770.00PRE1Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
YIL115C2.770.002810.02NUP159FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR008C_d14.306.91E-47YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YEL009C12.506.61E-36GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR007W11.201.59E-29TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YER090W11.108.90E-29TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR283C9.642.60E-22GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YKL211C8.372.80E-17TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGL026C7.631.15E-14TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YDR035W7.416.32E-14ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YNL335W_p7.106.23E-13DDI3_pProtein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants
YDR127W6.853.68E-12ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YDR354W6.824.54E-12TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YPL184C6.232.40E-10MRN1RNA-binding protein that may be involved in translational regulation; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation
YGL007W_d6.057.34E-10BRP1_dDubious ORF located in the upstream region of PMA1, deletion leads to polyamine resistance due to downregulation of PMA1
YHR125W_d5.181.12E-7YHR125W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKL157W5.071.97E-7APE2Aminopeptidase yscII; may have a role in obtaining leucine from dipeptide substrates; APE2 has a paralog, AAP1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1697
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.5870SGTC_18825615643 20.0 μMMiscellaneous22530750.197183TRP & mitochondrial translation
0.5820SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0735294
0.5730SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0735294
0.5590SGTC_18815652484 16.0 μMMiscellaneous22551290.2TRP & mitochondrial translation
0.5480SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.533333
0.5460SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.369565TRP & mitochondrial translation
0.5320SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0641026
0.5300SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0625
0.5140SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.1875
0.5130SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.0793651
0.5120SGTC_24885268135 45.7 μMMiscellaneous12703560.264151
0.5050SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)67107480.0169492
0.5010SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.057971
0.4990SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0694444
0.4990SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0352941

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_8920929-007629 μM0.5365855751038ChemDiv (Drug-like library)219.259662.42813TRP & mitochondrial translation
SGTC_12840929-004316 μM0.5365851239883ChemDiv (Drug-like library)223.2235432.14714
SGTC_205252651827.14 μM0.5365851239884Chembridge (Fragment library)223.2235432.14714
SGTC_6770929-0063136 μM0.5333331539583ChemDiv (Drug-like library)265.285041.90915
SGTC_380929-005158.27 μM0.5227271379719ChemDiv (Drug-like library)223.2235432.14714
SGTC_245553777129.75 μM0.5227272243908Miscellaneous219.259662.42813
SGTC_2165572719732.14 μM0.4772731361712Chembridge (Fragment library)211.26081.60414
SGTC_2102537781672.76 μM0.4444441379702Chembridge (Fragment library)195.19521.33714
SGTC_440929-005561.21 μM0.4130431379590ChemDiv (Drug-like library)239.678142.60613
SGTC_13661683-011321.9 μM0.395833708623ChemDiv (Drug-like library)224.233121.75925