st030840

2-(2-methoxy-4-prop-2-enylphenoxy)-N-[(Z)-(5-nitrofuran-2-yl)methylideneamino]acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1700
Screen concentration 7.9 μM
Source TimTec (Natural product derivative library)
PubChem CID 5499307
SMILES COC1=C(C=CC(=C1)CC=C)OCC(=O)NN=CC2=CC=C(O2)[N+](=O)[O-]
Standardized SMILES COc1cc(CC=C)ccc1OCC(=O)NN=Cc2oc(cc2)[N+](=O)[O-]
Molecular weight 359.3334
ALogP 2.92
H-bond donor count 1
H-bond acceptor count 7
Response signature PDR1

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.16
% growth inhibition (Hom. pool) 6.73


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5499307
Download HIP data (tab-delimited text)  (excel)
Gene:CCT4(YDL143W)|FD-Score:4.05|P-value:2.52E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:ENP1(YBR247C)|FD-Score:3.78|P-value:7.90E-5|Clearance:0.08||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:KOG1(YHR186C)|FD-Score:3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MCD4(YKL165C)|FD-Score:3.8|P-value:7.26E-5|Clearance:0.02||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM2(YBL023C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.01||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MPP10(YJR002W)|FD-Score:3.11|P-value:9.44E-4|Clearance:0.05||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:MTR3(YGR158C)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:ORC6(YHR118C)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.04||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:RPB5(YBR154C)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.13||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPB8(YOR224C)|FD-Score:3.19|P-value:7.15E-4|Clearance:0.08||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC19(YNL113W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.02||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPL33A(YPL143W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPL5(YPL131W)|FD-Score:4.05|P-value:2.56E-5|Clearance:0.25||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPS13(YDR064W)|FD-Score:3.53|P-value:2.06E-4|Clearance:0.11||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS3(YNL178W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RPT1(YKL145W)|FD-Score:4.74|P-value:1.05E-6|Clearance:0.65||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RRP4(YHR069C)|FD-Score:4.07|P-value:2.31E-5|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP43(YCR035C)|FD-Score:5.01|P-value:2.71E-7|Clearance:0.65||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:TAM41(YGR046W)|FD-Score:-4.37|P-value:6.29E-6|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:UTP10(YJL109C)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.09||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:YDL196W(YDL196W_d)|FD-Score:4.09|P-value:2.12E-5|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:CCT4(YDL143W)|FD-Score:4.05|P-value:2.52E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:ENP1(YBR247C)|FD-Score:3.78|P-value:7.90E-5|Clearance:0.08||SGD DESC:Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus Gene:KOG1(YHR186C)|FD-Score:3.7|P-value:1.08E-4|Clearance:0||SGD DESC:Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors Gene:MCD4(YKL165C)|FD-Score:3.8|P-value:7.26E-5|Clearance:0.02||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM2(YBL023C)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.01||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MPP10(YJR002W)|FD-Score:3.11|P-value:9.44E-4|Clearance:0.05||SGD DESC:Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p Gene:MTR3(YGR158C)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:ORC6(YHR118C)|FD-Score:3.58|P-value:1.74E-4|Clearance:0.04||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p Gene:RPB5(YBR154C)|FD-Score:3.4|P-value:3.33E-4|Clearance:0.13||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPB8(YOR224C)|FD-Score:3.19|P-value:7.15E-4|Clearance:0.08||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPC19(YNL113W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.02||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPL33A(YPL143W)|FD-Score:3.7|P-value:1.07E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication Gene:RPL5(YPL131W)|FD-Score:4.05|P-value:2.56E-5|Clearance:0.25||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPS13(YDR064W)|FD-Score:3.53|P-value:2.06E-4|Clearance:0.11||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS3(YNL178W)|FD-Score:3.19|P-value:7.13E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RPT1(YKL145W)|FD-Score:4.74|P-value:1.05E-6|Clearance:0.65||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance Gene:RRP4(YHR069C)|FD-Score:4.07|P-value:2.31E-5|Clearance:0.02||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP43(YCR035C)|FD-Score:5.01|P-value:2.71E-7|Clearance:0.65||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:TAM41(YGR046W)|FD-Score:-4.37|P-value:6.29E-6|Clearance:0||SGD DESC:Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition Gene:UTP10(YJL109C)|FD-Score:3.66|P-value:1.24E-4|Clearance:0.09||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance Gene:YDL196W(YDL196W_d)|FD-Score:4.09|P-value:2.12E-5|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5499307
Download HOP data (tab-delimited text)  (excel)
Gene:AFI1(YOR129C)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:AHP1(YLR109W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:BTS1(YPL069C)|FD-Score:5.29|P-value:6.20E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBC2(YPL178W)|FD-Score:6.74|P-value:8.09E-12||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:COG6(YNL041C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRF1(YDR223W)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:DAL80(YKR034W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DGR1(YNL130C-A_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:ERG2(YMR202W)|FD-Score:5.01|P-value:2.66E-7||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GAL11(YOL051W)|FD-Score:10.5|P-value:2.54E-26||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GSH1(YJL101C)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GTB1(YDR221W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:HO(YDL227C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p Gene:HRT3(YLR097C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:ILM1(YJR118C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:INP51(YIL002C)|FD-Score:5.23|P-value:8.64E-8||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:KGD1(YIL125W)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:KIN3(YAR018C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Nonessential serine/threonine protein kinase; possible role in DNA damage response Gene:MAC1(YMR021C)|FD-Score:5.37|P-value:3.95E-8||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MGR2(YPL098C)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MRP10(YDL045W-A)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPS16(YPL013C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRS3(YJL133W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:NRT1(YOR071C)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:NUP170(YBL079W)|FD-Score:5.43|P-value:2.80E-8||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OPI3(YJR073C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PCL8(YPL219W)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PDR1(YGL013C)|FD-Score:30.9|P-value:2.49E-210||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:14|P-value:1.18E-44||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIN4(YBL051C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PRO2(YOR323C)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTR2(YKR093W)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:QCR7(YDR529C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:REG1(YDR028C)|FD-Score:6.05|P-value:7.20E-10||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGA1(YOR127W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPN4(YDL020C)|FD-Score:4.29|P-value:8.93E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS8A(YBL072C)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SMF3(YLR034C)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SPT3(YDR392W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRY1(YKL218C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSN2(YDR443C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:STB5(YHR178W)|FD-Score:4.09|P-value:2.12E-5||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SWH1(YAR042W)|FD-Score:3.74|P-value:9.20E-5||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:TAZ1(YPR140W)|FD-Score:-3.93|P-value:4.24E-5||SGD DESC:Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome Gene:TEX1(YNL253W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:THP2(YHR167W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TMA7(YLR262C-A)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:22.4|P-value:8.35E-112||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMR1(YHL035C)|FD-Score:-4.44|P-value:4.41E-6||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS55(YJR044C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YBL028C(YBL028C)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:YDL124W(YDL124W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress Gene:YDL199C(YDL199C_p)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Putative transporter, member of the sugar porter family Gene:YIL028W(YIL028W_d)|FD-Score:-3.78|P-value:7.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL160C(YJL160C_p)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YLR211C(YLR211C_p)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YMR160W(YMR160W_p)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YNL324W(YNL324W_d)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL159C-A(YOL159C-A)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YOR248W(YOR248W_d)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL025C(YPL025C_d)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR084W(YPR084W_p)|FD-Score:3.92|P-value:4.51E-5||SGD DESC:Putative protein of unknown function Gene:AFI1(YOR129C)|FD-Score:4.69|P-value:1.35E-6||SGD DESC:Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p Gene:AHP1(YLR109W)|FD-Score:3.1|P-value:9.73E-4||SGD DESC:Thiol-specific peroxiredoxin, reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p Gene:BTS1(YPL069C)|FD-Score:5.29|P-value:6.20E-8||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBC2(YPL178W)|FD-Score:6.74|P-value:8.09E-12||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:COG6(YNL041C)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CRF1(YDR223W)|FD-Score:3.8|P-value:7.21E-5||SGD DESC:Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain Gene:DAL80(YKR034W)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DGR1(YNL130C-A_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:ERG2(YMR202W)|FD-Score:5.01|P-value:2.66E-7||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GAL11(YOL051W)|FD-Score:10.5|P-value:2.54E-26||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GSH1(YJL101C)|FD-Score:4.58|P-value:2.37E-6||SGD DESC:Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress Gene:GTB1(YDR221W)|FD-Score:3.37|P-value:3.81E-4||SGD DESC:Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress Gene:HO(YDL227C)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p Gene:HRT3(YLR097C)|FD-Score:3.5|P-value:2.34E-4||SGD DESC:Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies Gene:ILM1(YJR118C)|FD-Score:3.54|P-value:2.03E-4||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:INP51(YIL002C)|FD-Score:5.23|P-value:8.64E-8||SGD DESC:Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth Gene:KGD1(YIL125W)|FD-Score:-3.19|P-value:7.13E-4||SGD DESC:Component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA Gene:KIN3(YAR018C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Nonessential serine/threonine protein kinase; possible role in DNA damage response Gene:MAC1(YMR021C)|FD-Score:5.37|P-value:3.95E-8||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MGR2(YPL098C)|FD-Score:4.69|P-value:1.34E-6||SGD DESC:Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype) Gene:MRP10(YDL045W-A)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs Gene:MRPS16(YPL013C)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRS3(YJL133W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:NRT1(YOR071C)|FD-Score:-3.36|P-value:3.96E-4||SGD DESC:High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity Gene:NUP170(YBL079W)|FD-Score:5.43|P-value:2.80E-8||SGD DESC:Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication Gene:OPI3(YJR073C)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PCL8(YPL219W)|FD-Score:-3.12|P-value:9.01E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PDR1(YGL013C)|FD-Score:30.9|P-value:2.49E-210||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:14|P-value:1.18E-44||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PIN4(YBL051C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage Gene:PRO2(YOR323C)|FD-Score:3.12|P-value:9.04E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTR2(YKR093W)|FD-Score:4.67|P-value:1.50E-6||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:QCR7(YDR529C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:REG1(YDR028C)|FD-Score:6.05|P-value:7.20E-10||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RGA1(YOR127W)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RPN4(YDL020C)|FD-Score:4.29|P-value:8.93E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS8A(YBL072C)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SMF3(YLR034C)|FD-Score:-3.1|P-value:9.65E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SPT3(YDR392W)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters Gene:SRY1(YKL218C)|FD-Score:3.35|P-value:4.02E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSN2(YDR443C)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation Gene:STB5(YHR178W)|FD-Score:4.09|P-value:2.12E-5||SGD DESC:Transcription factor, involved in regulating multidrug resistance and oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro Gene:SWH1(YAR042W)|FD-Score:3.74|P-value:9.20E-5||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:TAZ1(YPR140W)|FD-Score:-3.93|P-value:4.24E-5||SGD DESC:Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome Gene:TEX1(YNL253W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:THP2(YHR167W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TMA7(YLR262C-A)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress Gene:TSC3(YBR058C-A)|FD-Score:22.4|P-value:8.35E-112||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VMR1(YHL035C)|FD-Score:-4.44|P-value:4.41E-6||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS55(YJR044C)|FD-Score:3.1|P-value:9.71E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YBL028C(YBL028C)|FD-Score:-3.12|P-value:8.91E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:YDL124W(YDL124W)|FD-Score:4.7|P-value:1.29E-6||SGD DESC:NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress Gene:YDL199C(YDL199C_p)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Putative transporter, member of the sugar porter family Gene:YIL028W(YIL028W_d)|FD-Score:-3.78|P-value:7.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL160C(YJL160C_p)|FD-Score:-3.28|P-value:5.22E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YLR211C(YLR211C_p)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YMR160W(YMR160W_p)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress Gene:YNL324W(YNL324W_d)|FD-Score:-3.16|P-value:7.84E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL159C-A(YOL159C-A)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species Gene:YOR248W(YOR248W_d)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL025C(YPL025C_d)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR084W(YPR084W_p)|FD-Score:3.92|P-value:4.51E-5||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCR035C5.012.71E-70.65RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
YKL145W4.741.05E-60.65RPT1One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
YDL196W_d4.092.12E-50.02YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YHR069C4.072.31E-50.02RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YDL143W4.052.52E-50.00CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YPL131W4.052.56E-50.25RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YKL165C3.807.26E-50.02MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YBR247C3.787.90E-50.08ENP1Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
YPL143W3.701.07E-40.00RPL33ARibosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
YHR186C3.701.08E-48.56E-4KOG1Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
YNL113W3.701.08E-40.02RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YBL023C3.681.18E-40.01MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YJL109C3.661.24E-40.09UTP10Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
YHR118C3.581.74E-40.04ORC6Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
YDR064W3.532.06E-40.11RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL013C30.902.49E-210PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YBR058C-A22.408.35E-112TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGL025C14.001.18E-44PGD1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
YOL051W10.502.54E-26GAL11Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
YPL178W6.748.09E-12CBC2Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif
YDR028C6.057.20E-10REG1Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p
YBL079W5.432.80E-8NUP170Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
YMR021C5.373.95E-8MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YPL069C5.296.20E-8BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YIL002C5.238.64E-8INP51Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
YMR202W5.012.66E-7ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YDL124W4.701.29E-6YDL124WNADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress
YPL098C4.691.34E-6MGR2Subunit of the TIM23 translocase complex; acts to couple Tim21p with the core Tim23 translocase; absolutely required for mitochondrial import of presequence-containing proteins at elevated temperature; required for viability of cells lacking the mitochondrial genome (petite-negative phenotype)
YOR129C4.691.35E-6AFI1Arf3p polarization-specific docking factor, required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p
YKR093W4.671.50E-6PTR2Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p

GO enrichment analysis for SGTC_1700
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4571.85E-302SGTC_5274476-4215 268.0 μMChemDiv (Drug-like library)44187540.0833333PDR1
0.4231.02E-254SGTC_295k015-0027 31.0 μMChemDiv (Drug-like library)38874240.170732PDR1
0.4192.68E-249SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.1375TSC3-RPN4
0.4091.12E-236SGTC_1139k015-0024 46.9 μMChemDiv (Drug-like library)27666520.0804598PDR1
0.3936.22E-217SGTC_6641222-0054 14.9 μMChemDiv (Drug-like library)68139910.0909091sphingolipid biosynthesis & PDR1
0.3871.16E-210SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.097561TSC3-RPN4
0.3781.61E-199SGTC_32419133421 49.5 μMChembridge (Drug-like library)170182210.0666667PDR1
0.3663.34E-186SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0843373TSC3-RPN4
0.3611.34E-181SGTC_30259087895 49.5 μMChembridge (Drug-like library)171736410.0927835PDR1
0.3592.19E-179SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.09375TSC3-RPN4
0.3571.56E-176SGTC_2508celastrol 40.5 μMMicrosource (Natural product library)1227240.0571429PDR1
0.3557.17E-175SGTC_12770873-0036 22.0 μMChemDiv (Drug-like library)59657350.0786517PDR1
0.3542.54E-174SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.0537634PDR1
0.3534.57E-173SGTC_5481436-0017 110.0 μMChemDiv (Drug-like library)96771370.166667TSC3-RPN4
0.3531.15E-172SGTC_12430416-0003 5.7 μMChemDiv (Drug-like library)21419770.0379747PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_7531322-0200289 μM0.4142865403907ChemDiv (Drug-like library)275.216961.85526
SGTC_12001643-019318.4 μM0.3974365403974ChemDiv (Drug-like library)347.753063.56315
SGTC_7911348-1292225 μM0.3924055437054ChemDiv (Drug-like library)465.294543.2917
SGTC_12072099-0101141 μM0.3815795837381ChemDiv (Drug-like library)378.177483.16115
SGTC_24675474540200 μM0.3521135413519Miscellaneous276.718222.92313
SGTC_2669eugenol100 μM0.3492063314Microsource (Natural product library)164.201082.57912
SGTC_12011889-337245.9 μM0.3108115392199ChemDiv (Drug-like library)282.337023.49113Golgi
SGTC_9252064-127259.6 μM0.3037976815817ChemDiv (Drug-like library)333.726481.44825
SGTC_13231348-135111.2 μM0.2962966989654ChemDiv (Drug-like library)336.304882.617
SGTC_587k089-000357 μM0.2959189659696ChemDiv (Drug-like library)447.446642.62817