st028805

ethyl 2-(carbamoylamino)-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophene-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1704
Screen concentration 70.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 2876101
SMILES CCOC(=O)C1=C(SC2=C1CCCCC2)NC(=O)N
Standardized SMILES CCOC(=O)c1c2CCCCCc2sc1NC(=O)N
Molecular weight 282.3586
ALogP 2.57
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.8
% growth inhibition (Hom. pool) 7.31


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2876101
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:3.86|P-value:5.58E-5|Clearance:0.26||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:EMW1(YNL313C)|FD-Score:3.46|P-value:2.75E-4|Clearance:0.26||SGD DESC:Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress Gene:ERV1(YGR029W)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:KEG1(YFR042W)|FD-Score:-3.13|P-value:8.78E-4|Clearance:0||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:NOP14(YDL148C)|FD-Score:3.15|P-value:8.13E-4|Clearance:0.05||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PRP19(YLL036C)|FD-Score:4.12|P-value:1.88E-5|Clearance:0.26||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RNA1(YMR235C)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.26||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPL25(YOL127W)|FD-Score:4.02|P-value:2.89E-5|Clearance:0.26||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RTP1(YMR185W_p)|FD-Score:-3.13|P-value:8.72E-4|Clearance:0||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SPT6(YGR116W)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.26||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SUP35(YDR172W)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.26||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TAF10(YDR167W)|FD-Score:4.14|P-value:1.71E-5|Clearance:0.26||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:YAL034C-B(YAL034C-B_d)|FD-Score:3.1|P-value:9.65E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL016C(YDL016C_d)|FD-Score:-3.19|P-value:7.08E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YEF3(YLR249W)|FD-Score:3.2|P-value:6.93E-4|Clearance:0.04||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YNL114C(YNL114C_d)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YRB2(YIL063C)|FD-Score:3.81|P-value:7.07E-5|Clearance:0.26||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential Gene:ACT1(YFL039C)|FD-Score:3.86|P-value:5.58E-5|Clearance:0.26||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:EMW1(YNL313C)|FD-Score:3.46|P-value:2.75E-4|Clearance:0.26||SGD DESC:Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress Gene:ERV1(YGR029W)|FD-Score:-3.28|P-value:5.14E-4|Clearance:0||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:KEG1(YFR042W)|FD-Score:-3.13|P-value:8.78E-4|Clearance:0||SGD DESC:Integral membrane protein of the ER; physically interacts with Kre6p; has a role in the synthesis of beta-1,6-glucan in the cell wall; required for cell viability Gene:NOP14(YDL148C)|FD-Score:3.15|P-value:8.13E-4|Clearance:0.05||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PRP19(YLL036C)|FD-Score:4.12|P-value:1.88E-5|Clearance:0.26||SGD DESC:Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain Gene:RNA1(YMR235C)|FD-Score:3.57|P-value:1.79E-4|Clearance:0.26||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPL25(YOL127W)|FD-Score:4.02|P-value:2.89E-5|Clearance:0.26||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RTP1(YMR185W_p)|FD-Score:-3.13|P-value:8.72E-4|Clearance:0||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SPT6(YGR116W)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.26||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SUP35(YDR172W)|FD-Score:3.57|P-value:1.77E-4|Clearance:0.26||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TAF10(YDR167W)|FD-Score:4.14|P-value:1.71E-5|Clearance:0.26||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:YAL034C-B(YAL034C-B_d)|FD-Score:3.1|P-value:9.65E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL016C(YDL016C_d)|FD-Score:-3.19|P-value:7.08E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:YEF3(YLR249W)|FD-Score:3.2|P-value:6.93E-4|Clearance:0.04||SGD DESC:Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication Gene:YNL114C(YNL114C_d)|FD-Score:3.16|P-value:7.98E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit Gene:YRB2(YIL063C)|FD-Score:3.81|P-value:7.07E-5|Clearance:0.26||SGD DESC:Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2876101
Download HOP data (tab-delimited text)  (excel)
Gene:AIM46(YHR199C)|FD-Score:4.93|P-value:4.08E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARA1(YBR149W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARO80(YDR421W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:BFR1(YOR198C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BST1(YFL025C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CHS7(YHR142W)|FD-Score:5.51|P-value:1.80E-8||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:DCI1(YOR180C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:ERP3(YDL018C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:ERP4(YOR016C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCN4(YEL009C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GSH2(YOL049W)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:HCS1(YKL017C)|FD-Score:3.85|P-value:5.79E-5||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:MPA43(YNL249C)|FD-Score:-3.77|P-value:8.28E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRPL20(YKR085C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NHP10(YDL002C)|FD-Score:4.35|P-value:6.83E-6||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NHX1(YDR456W)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:PEP3(YLR148W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PMD1(YER132C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PUF3(YLL013C)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:REV7(YIL139C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RHO2(YNL090W)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RPL1A(YPL220W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:SCS22(YBL091C-A)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SLX8(YER116C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SMF3(YLR034C)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:TDH1(YJL052W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TIR2(YOR010C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis Gene:UBP11(YKR098C)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:URM1(YIL008W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAC14(YLR386W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS30(YPL120W)|FD-Score:5.01|P-value:2.66E-7||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:YCR064C(YCR064C_d)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YGR259C(YGR259C_d)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YJL152W(YJL152W_d)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL162C(YKL162C_p)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YKR045C(YKR045C_p)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR036C(YLR036C_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR224W(YLR224W_p)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene Gene:AIM46(YHR199C)|FD-Score:4.93|P-value:4.08E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARA1(YBR149W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARO80(YDR421W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids Gene:BFR1(YOR198C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BST1(YFL025C)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CHS7(YHR142W)|FD-Score:5.51|P-value:1.80E-8||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:DCI1(YOR180C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed Gene:ERP3(YDL018C)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:ERP4(YOR016C)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:GCN4(YEL009C)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GSH2(YOL049W)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock Gene:HCS1(YKL017C)|FD-Score:3.85|P-value:5.79E-5||SGD DESC:Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities Gene:MPA43(YNL249C)|FD-Score:-3.77|P-value:8.28E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:MRPL20(YKR085C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NHP10(YDL002C)|FD-Score:4.35|P-value:6.83E-6||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NHX1(YDR456W)|FD-Score:3.94|P-value:4.14E-5||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:PEP3(YLR148W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PMD1(YER132C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions Gene:PUF3(YLL013C)|FD-Score:-3.11|P-value:9.26E-4||SGD DESC:Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins Gene:REV7(YIL139C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RHO2(YNL090W)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly Gene:RPL1A(YPL220W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:SCS22(YBL091C-A)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; similar to D. melanogaster inturned protein; SCS22 has a paralog, SCS2, that arose from the whole genome duplication Gene:SLX8(YER116C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SMF3(YLR034C)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:TDH1(YJL052W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TIR2(YOR010C)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis Gene:UBP11(YKR098C)|FD-Score:3.87|P-value:5.39E-5||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:URM1(YIL008W)|FD-Score:3.43|P-value:3.05E-4||SGD DESC:Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response Gene:VAC14(YLR386W)|FD-Score:4.01|P-value:3.09E-5||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:VPS30(YPL120W)|FD-Score:5.01|P-value:2.66E-7||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:YCR064C(YCR064C_d)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YGR259C(YGR259C_d)|FD-Score:3.12|P-value:9.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YJL152W(YJL152W_d)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL162C(YKL162C_p)|FD-Score:3.98|P-value:3.42E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YKR045C(YKR045C_p)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm Gene:YLR036C(YLR036C_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Putative protein of unknown function predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein Gene:YLR224W(YLR224W_p)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:F-box protein and component of SCF ubiquitin ligase complexes involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; YLR224W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR167W4.141.71E-50.26TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YLL036C4.121.88E-50.26PRP19Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain
YOL127W4.022.89E-50.26RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YFL039C3.865.58E-50.26ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YIL063C3.817.07E-50.26YRB2Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential
YGR116W3.681.18E-40.26SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YDR172W3.571.77E-40.26SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YMR235C3.571.79E-40.26RNA1GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
YNL313C3.462.75E-40.26EMW1Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress
YLR249W3.206.93E-40.04YEF3Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
YNL114C_d3.167.98E-40.01YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
YDL148C3.158.13E-40.05NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YAL034C-B_d3.109.65E-40.06YAL034C-B_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YFL005W3.040.001190.05SEC4Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
YKL018W2.990.001410.02SWD2Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lys 4 and is involved in telomeric silencing; subunit of CPF (cleavage and polyadenylation factor), a complex involved in RNAP II transcription termination

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR142W5.511.80E-8CHS7Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress
YLR034C5.022.62E-7SMF3Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress
YPL120W5.012.66E-7VPS30Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
YHR199C4.934.08E-7AIM46Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YDL002C4.356.83E-6NHP10Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair
YOL049W4.102.10E-5GSH2Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
YLR386W4.013.09E-5VAC14Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p
YKL162C_p3.983.42E-5YKL162C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
YER116C3.963.72E-5SLX8Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress
YDR456W3.944.14E-5NHX1Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
YKR098C3.875.39E-5UBP11Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins
YKL017C3.855.79E-5HCS1Hexameric DNA polymerase alpha-associated DNA helicase A involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities
YBR149W3.721.01E-4ARA1NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product
YLR148W3.671.22E-4PEP3Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
YOR180C3.641.36E-4DCI1Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed

GO enrichment analysis for SGTC_1704
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0863.28E-11SGTC_2064546-0025 72.0 μMChemDiv (Drug-like library)66207450.0769231NEO1
0.0823.31E-10SGTC_23276189656 200.0 μMChembridge (Fragment library)53490880.0285714
0.0691.13E-7SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.0694444NEO1
0.0631.18E-6SGTC_28207989630 71.4 μMChembridge (Drug-like library)29840980.103896ERG2
0.0631.42E-6SGTC_1618st003135 44.5 μMTimTec (Natural product derivative library)12706150.24
0.0621.56E-6SGTC_1066ketoconazole 628.0 nMNIH Clinical Collection4562010.0792079
0.0603.93E-6SGTC_8191013-0258 27.7 μMChemDiv (Drug-like library)57531980.075
0.0595.98E-6SGTC_28739040596 71.4 μMChembridge (Drug-like library)64638630.144737
0.0571.32E-5SGTC_478N-arachidonylglycine 10.0 μMICCB bioactive library52833890.107692
0.0571.36E-5SGTC_9591222-0044 107.0 μMChemDiv (Drug-like library)60836120.109375
0.0561.55E-5SGTC_890302-0250 142.1 μMChemDiv (Drug-like library)42035180.0793651
0.0561.57E-5SGTC_1781st038725 66.3 μMTimTec (Natural product derivative library)42702750.0416667NEO1
0.0552.35E-5SGTC_9403232-1287 54.8 μMChemDiv (Drug-like library)15854930.0987654NEO1
0.0552.60E-5SGTC_24265582046 32.2 μMMiscellaneous22519340.111111
0.0534.34E-5SGTC_29839022671 13.0 μMChembridge (Drug-like library)64051610.068181860S ribosome export

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2937904475311.33 μM0.6538466465530Chembridge (Drug-like library)393.543443.0921660S ribosome export
SGTC_9751335-004628.5 μM0.47368416486740ChemDiv (Drug-like library)374.250142.67915RPP1 & pyrimidine depletion
SGTC_1756st04155835.5 μM0.4459463955792TimTec (Natural product derivative library)564.649284.77928
SGTC_309k408-00511.48 μM0.4411763640463ChemDiv (Drug-like library)505.650043.32218
SGTC_3216912971649.47 μM0.4385968056934Chembridge (Drug-like library)310.368721.40815RPP1 & pyrimidine depletion
SGTC_2833900131545.46 μM0.4333332989378Chembridge (Drug-like library)358.411522.55425amide catabolism
SGTC_11333473-024750 μM0.40678711311ChemDiv (Drug-like library)308.422343.35625RSC complex & mRNA processing
SGTC_9133487-1245167 μM0.389834016153ChemDiv (Drug-like library)349.831844.53424
SGTC_10134112-3890241 μM0.387097705171ChemDiv (Drug-like library)331.386183.39725TSC3-RPN4
SGTC_8034488-1585147 μM0.383333747204ChemDiv (Drug-like library)315.386783.924