st031321

(E)-1-(3-aminophenyl)-3-(3,4-dichlorophenyl)prop-2-en-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1706
Screen concentration 10.0 μM
Source TimTec (Natural product derivative library)
PubChem CID 6103185
SMILES C1=CC(=CC(=C1)N)C(=O)C=CC2=CC(=C(C=C2)Cl)Cl
Standardized SMILES Nc1cccc(c1)C(=O)C=Cc2ccc(Cl)c(Cl)c2
Molecular weight 292.1599
ALogP 4.28
H-bond donor count 1
H-bond acceptor count 2
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.6
% growth inhibition (Hom. pool) 8.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6103185
Download HIP data (tab-delimited text)  (excel)
Gene:CWC24(YLR323C)|FD-Score:-3.39|P-value:3.45E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:HSP60(YLR259C)|FD-Score:-4.01|P-value:3.08E-5|Clearance:0||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:RPP1(YHR062C)|FD-Score:3.85|P-value:5.93E-5|Clearance:0.48||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRB1(YMR131C)|FD-Score:5.46|P-value:2.40E-8|Clearance:1.47||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SEC23(YPR181C)|FD-Score:3.99|P-value:3.27E-5|Clearance:0.14||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:VTI1(YMR197C)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.28||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDL152W(YDL152W_d)|FD-Score:-4.54|P-value:2.83E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:CWC24(YLR323C)|FD-Score:-3.39|P-value:3.45E-4|Clearance:0||SGD DESC:Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p Gene:HSP60(YLR259C)|FD-Score:-4.01|P-value:3.08E-5|Clearance:0||SGD DESC:Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated Gene:RPP1(YHR062C)|FD-Score:3.85|P-value:5.93E-5|Clearance:0.48||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRB1(YMR131C)|FD-Score:5.46|P-value:2.40E-8|Clearance:1.47||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SEC23(YPR181C)|FD-Score:3.99|P-value:3.27E-5|Clearance:0.14||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:VTI1(YMR197C)|FD-Score:3.37|P-value:3.81E-4|Clearance:0.28||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDL152W(YDL152W_d)|FD-Score:-4.54|P-value:2.83E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6103185
Download HOP data (tab-delimited text)  (excel)
Gene:AAD6(YFL056C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ARG8(YOL140W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:ARO1(YDR127W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BTS1(YPL069C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF40(YNL288W)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CAJ1(YER048C)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CKI1(YLR133W)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CSR1(YLR380W)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CTS2(YDR371W_p)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:CUR1(YPR158W)|FD-Score:4.76|P-value:9.46E-7||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:CUS2(YNL286W)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:FET4(YMR319C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GLE2(YER107C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HLR1(YDR528W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A; <br>HLR1 has a paralog, LRE1, that arose from the whole genome duplication Gene:LNP1(YHR192W_p)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MLH3(YPL164C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MOD5(YOR274W)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MTC2(YKL098W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NTE1(YML059C)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:OMS1(YDR316W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PAU24(YBR301W)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PDR1(YGL013C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:RAS1(YOR101W)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RPA12(YJR063W)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPN4(YDL020C)|FD-Score:3.8|P-value:7.37E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SPF1(YEL031W)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:STP22(YCL008C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SWA2(YDR320C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TEX1(YNL253W)|FD-Score:-4.06|P-value:2.48E-5||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:TPK2(YPL203W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRP1(YDR007W)|FD-Score:6.32|P-value:1.28E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:12.9|P-value:1.25E-38||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URE2(YNL229C)|FD-Score:6.5|P-value:3.95E-11||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:XKS1(YGR194C)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains Gene:YAP1801(YHR161C)|FD-Score:4.86|P-value:5.93E-7||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YCR007C(YCR007C_p)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR269C(YDR269C_d)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER158C(YER158C_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YGL072C(YGL072C_d)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR168C(YGR168C_p)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHR139C-A(YHR139C-A_d)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML089C(YML089C_d)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YPK1(YKL126W)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL245W(YPL245W_p)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm Gene:AAD6(YFL056C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ARG8(YOL140W)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:ARO1(YDR127W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BTS1(YPL069C)|FD-Score:4.23|P-value:1.19E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CAF40(YNL288W)|FD-Score:-3.26|P-value:5.48E-4||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CAJ1(YER048C)|FD-Score:4.58|P-value:2.35E-6||SGD DESC:Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly Gene:CKI1(YLR133W)|FD-Score:3.77|P-value:8.15E-5||SGD DESC:Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication Gene:CSR1(YLR380W)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress Gene:CTS2(YDR371W_p)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:CUR1(YPR158W)|FD-Score:4.76|P-value:9.46E-7||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:CUS2(YNL286W)|FD-Score:-3.37|P-value:3.81E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:FET4(YMR319C)|FD-Score:3.18|P-value:7.41E-4||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:GLE2(YER107C)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HLR1(YDR528W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A; <br>HLR1 has a paralog, LRE1, that arose from the whole genome duplication Gene:LNP1(YHR192W_p)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:MLH3(YPL164C)|FD-Score:-3.35|P-value:4.02E-4||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MOD5(YOR274W)|FD-Score:-3.31|P-value:4.60E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MTC2(YKL098W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Protein of unknown function; mtc2 is synthetically sick with cdc13-1 Gene:NTE1(YML059C)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Serine esterase, homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes Gene:OMS1(YDR316W)|FD-Score:3.19|P-value:7.06E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PAU24(YBR301W)|FD-Score:3.75|P-value:8.86E-5||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:PDR1(YGL013C)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:3.21|P-value:6.61E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:RAS1(YOR101W)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in cell proliferation; localized to the plasma membrane; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress Gene:RPA12(YJR063W)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPN4(YDL020C)|FD-Score:3.8|P-value:7.37E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SHE4(YOR035C)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SPF1(YEL031W)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:STP22(YCL008C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype Gene:SWA2(YDR320C)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TEX1(YNL253W)|FD-Score:-4.06|P-value:2.48E-5||SGD DESC:Protein involved in mRNA export, component of the transcription export (TREX) complex Gene:TPK2(YPL203W)|FD-Score:3.11|P-value:9.39E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRP1(YDR007W)|FD-Score:6.32|P-value:1.28E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TSC3(YBR058C-A)|FD-Score:12.9|P-value:1.25E-38||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:URE2(YNL229C)|FD-Score:6.5|P-value:3.95E-11||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:XKS1(YGR194C)|FD-Score:3.12|P-value:9.07E-4||SGD DESC:Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains Gene:YAP1801(YHR161C)|FD-Score:4.86|P-value:5.93E-7||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YCR007C(YCR007C_p)|FD-Score:3.11|P-value:9.25E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR269C(YDR269C_d)|FD-Score:-3.09|P-value:9.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER158C(YER158C_p)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YGL072C(YGL072C_d)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YGR168C(YGR168C_p)|FD-Score:3.24|P-value:5.94E-4||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHR139C-A(YHR139C-A_d)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML089C(YML089C_d)|FD-Score:-3.28|P-value:5.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage Gene:YPK1(YKL126W)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL245W(YPL245W_p)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR131C5.462.40E-81.47RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YPR181C3.993.27E-50.14SEC23GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
YHR062C3.855.93E-50.48RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YMR197C3.373.81E-40.28VTI1Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways
YLR298C3.090.001010.06YHC1Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site
YEL035C_p3.030.001230.01UTR5_pProtein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions
YER048W-A3.020.001270.05ISD11Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis
YGR065C2.970.001480.06VHT1High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
YJL025W2.910.001790.06RRN7Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
YGR048W2.860.002140.03UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YPL020C2.820.002370.07ULP1Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
YDR365C2.760.002900.02ESF1Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
YJL085W2.740.003110.01EXO70Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
YDL064W2.730.003160.07UBC9SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)
YJR045C2.660.003860.06SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A12.901.25E-38TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YNL229C6.503.95E-11URE2Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
YDR007W6.321.28E-10TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YKL126W5.591.16E-8YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YJR063W5.571.24E-8RPA12RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
YHR139C-A_d5.131.48E-7YHR139C-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YHR161C4.865.93E-7YAP1801Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication
YPR158W4.769.46E-7CUR1Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication
YGL013C4.721.16E-6PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YER048C4.582.35E-6CAJ1Nuclear type II J heat shock protein of the E. coli dnaJ family, contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly
YPL069C4.231.19E-5BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YKL098W4.102.09E-5MTC2Protein of unknown function; mtc2 is synthetically sick with cdc13-1
YGL072C_d4.062.44E-5YGL072C_dDubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
YEL031W3.846.22E-5SPF1P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
YDL020C3.807.37E-5RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress

GO enrichment analysis for SGTC_1706
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4044.92E-231SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.333333TSC3-RPN4
0.3903.97E-214SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.307692TSC3-RPN4
0.3833.84E-206SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0645161TSC3-RPN4
0.3821.22E-204SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.202899TSC3-RPN4
0.3648.02E-185SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.277778TSC3-RPN4
0.3567.53E-176SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0645161TSC3-RPN4
0.3401.54E-159SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.0819672TSC3-RPN4
0.3384.45E-158SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.126984TSC3-RPN4
0.3341.96E-153SGTC_2701st077767 14.7 μMTimTec (Natural product derivative library)57211880.22807
0.3265.12E-146SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.101449TSC3-RPN4
0.3157.54E-136SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0945946
0.3151.06E-135SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0806452TSC3-RPN4
0.3141.54E-135SGTC_1726st035556 14.6 μMTimTec (Natural product derivative library)59194390.32
0.3123.30E-133SGTC_9262268-1401 59.4 μMChemDiv (Drug-like library)29315780.114286TSC3-RPN4
0.3094.67E-131SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.142857TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1699st03131829.05 μM0.6136365340658TimTec (Natural product derivative library)269.36143.49713
SGTC_1795st04954562.3 μM0.5531915291882TimTec (Natural product derivative library)321.15484.79803TSC3-RPN4
SGTC_1608st00147340.5 μM0.476197292267TimTec (Natural product derivative library)242.700244.36601
SGTC_7991310-0557151 μM0.4081635354492ChemDiv (Drug-like library)252.264683.4703superoxide
SGTC_20054024620188 μM0.369565236743Chembridge (Fragment library)179.215721.66513
SGTC_1828st05581149 μM0.3606565877589TimTec (Natural product derivative library)407.803265.69205
SGTC_26044'-hydroxychalcone11.32 μM0.3478265282362Microsource (Natural product library)224.254583.4612
SGTC_1737st03555769.7 μM0.3461545716916TimTec (Natural product derivative library)286.75284.69802
SGTC_1603st00204567.8 μM0.3333335887985TimTec (Natural product derivative library)295.1357635.23602TSC3-RPN4
SGTC_7211187-1332279 μM0.32653125730ChemDiv (Drug-like library)281.13733.96522cell wall signaling