st031758

methyl 3-[[(E)-3-(1,3-benzodioxol-5-yl)prop-2-enoyl]amino]benzoate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1710
Screen concentration 61.5 μM
Source TimTec (Natural product derivative library)
PubChem CID 5394341
SMILES COC(=O)C1=CC(=CC=C1)NC(=O)C=CC2=CC3=C(C=C2)OCO3
Standardized SMILES COC(=O)c1cccc(NC(=O)C=Cc2ccc3OCOc3c2)c1
Molecular weight 325.3154
ALogP 2.71
H-bond donor count 1
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 10.93
% growth inhibition (Hom. pool) 7.31


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5394341
Download HIP data (tab-delimited text)  (excel)
Gene:BUR6(YER159C)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.11||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:DML1(YMR211W)|FD-Score:3.87|P-value:5.55E-5|Clearance:0.05||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:GLC7(YER133W)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.25||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HTS1(YPR033C)|FD-Score:3.32|P-value:4.51E-4|Clearance:0.05||SGD DESC:Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome Gene:JAC1(YGL018C)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.11||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:LCD1(YDR499W)|FD-Score:3.16|P-value:7.79E-4|Clearance:0.02||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:MIM1(YOL026C)|FD-Score:3.17|P-value:7.69E-4|Clearance:0||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:NCB2(YDR397C)|FD-Score:5.13|P-value:1.48E-7|Clearance:1.13||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta Gene:NUP82(YJL061W)|FD-Score:-3.45|P-value:2.84E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:POL30(YBR088C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.02||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:RNR2(YJL026W)|FD-Score:-3.69|P-value:1.12E-4|Clearance:0||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPF2(YKR081C)|FD-Score:3.81|P-value:6.88E-5|Clearance:0.13||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RRN7(YJL025W)|FD-Score:-3.43|P-value:3.01E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RSC6(YCR052W)|FD-Score:-3.9|P-value:4.87E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC13(YLR208W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.11||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SLD2(YKL108W)|FD-Score:4|P-value:3.23E-5|Clearance:0.13||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress Gene:BUR6(YER159C)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.11||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:DML1(YMR211W)|FD-Score:3.87|P-value:5.55E-5|Clearance:0.05||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:GLC7(YER133W)|FD-Score:3.57|P-value:1.80E-4|Clearance:0.25||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HTS1(YPR033C)|FD-Score:3.32|P-value:4.51E-4|Clearance:0.05||SGD DESC:Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome Gene:JAC1(YGL018C)|FD-Score:3.13|P-value:8.80E-4|Clearance:0.11||SGD DESC:Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix Gene:LCD1(YDR499W)|FD-Score:3.16|P-value:7.79E-4|Clearance:0.02||SGD DESC:Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress Gene:MIM1(YOL026C)|FD-Score:3.17|P-value:7.69E-4|Clearance:0||SGD DESC:Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex Gene:NCB2(YDR397C)|FD-Score:5.13|P-value:1.48E-7|Clearance:1.13||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta Gene:NUP82(YJL061W)|FD-Score:-3.45|P-value:2.84E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:POL30(YBR088C)|FD-Score:3.15|P-value:8.28E-4|Clearance:0.02||SGD DESC:Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair Gene:RNR2(YJL026W)|FD-Score:-3.69|P-value:1.12E-4|Clearance:0||SGD DESC:Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits Gene:RPF2(YKR081C)|FD-Score:3.81|P-value:6.88E-5|Clearance:0.13||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RRN7(YJL025W)|FD-Score:-3.43|P-value:3.01E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RSC6(YCR052W)|FD-Score:-3.9|P-value:4.87E-5|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC13(YLR208W)|FD-Score:3.68|P-value:1.16E-4|Clearance:0.11||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SLD2(YKL108W)|FD-Score:4|P-value:3.23E-5|Clearance:0.13||SGD DESC:Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5394341
Download HOP data (tab-delimited text)  (excel)
Gene:ARO1(YDR127W)|FD-Score:4.65|P-value:1.67E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BCK1(YJL095W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BTS1(YPL069C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CRC1(YOR100C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation Gene:DAL81(YIR023W)|FD-Score:6.86|P-value:3.44E-12||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DDI3(YNL335W_p)|FD-Score:4.39|P-value:5.67E-6||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:ERG2(YMR202W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GDS1(YOR355W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:IGO2(YHR132W-A)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:MAC1(YMR021C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MET31(YPL038W)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:NAT1(YDL040C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NKP2(YLR315W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:PCL8(YPL219W)|FD-Score:4.52|P-value:3.14E-6||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PEX29(YDR479C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PFK2(YMR205C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PTP1(YDL230W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:REG1(YDR028C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM4(YHL024W)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:SHC1(YER096W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SIP1(YDR422C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SNF12(YNR023W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPR3(YGR059W)|FD-Score:-3.77|P-value:8.09E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SRC1(YML034W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSK2(YNR031C)|FD-Score:5.04|P-value:2.30E-7||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SYN8(YAL014C)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TRP1(YDR007W)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:8.52|P-value:7.66E-18||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.84|P-value:4.85E-19||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.81|P-value:7.40E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBP8(YMR223W)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:VAM3(YOR106W)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VFA1(YER128W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:YCH1(YGR203W)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR008C(YDR008C_d)|FD-Score:5.81|P-value:3.11E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL068C(YJL068C)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YJR142W(YJR142W_p)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YLR126C(YLR126C_p)|FD-Score:5.17|P-value:1.17E-7||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YLR271W(YLR271W_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR422W(YLR422W_p)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YNR066C(YNR066C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:YPL119C-A(YPL119C-A_p)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:ARO1(YDR127W)|FD-Score:4.65|P-value:1.67E-6||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:BCK1(YJL095W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BTS1(YPL069C)|FD-Score:-3.21|P-value:6.57E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CRC1(YOR100C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Mitochondrial inner membrane carnitine transporter, required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation Gene:DAL81(YIR023W)|FD-Score:6.86|P-value:3.44E-12||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DDI3(YNL335W_p)|FD-Score:4.39|P-value:5.67E-6||SGD DESC:Protein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants Gene:ERG2(YMR202W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GDS1(YOR355W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein of unknown function, required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:IGO2(YHR132W-A)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo1p Gene:MAC1(YMR021C)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MET31(YPL038W)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:NAT1(YDL040C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NKP2(YLR315W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2 Gene:PCL8(YPL219W)|FD-Score:4.52|P-value:3.14E-6||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PEX29(YDR479C)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress Gene:PFK2(YMR205C)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PTP1(YDL230W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation Gene:REG1(YDR028C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM4(YHL024W)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:SHC1(YER096W)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication Gene:SIP1(YDR422C)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Alternate beta-subunit of the Snf1p kinase complex, may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions Gene:SNF12(YNR023W)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:73 kDa subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; homolog of Rsc6p subunit of the RSC chromatin remodeling complex; relocates to the cytosol under hypoxic conditions; deletion mutants are temperature-sensitive Gene:SPR3(YGR059W)|FD-Score:-3.77|P-value:8.09E-5||SGD DESC:Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI Gene:SRC1(YML034W)|FD-Score:3.35|P-value:4.05E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSK2(YNR031C)|FD-Score:5.04|P-value:2.30E-7||SGD DESC:MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress Gene:SYN8(YAL014C)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TRP1(YDR007W)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:8.52|P-value:7.66E-18||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:8.84|P-value:4.85E-19||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.51|P-value:2.26E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:4.81|P-value:7.40E-7||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:UBP8(YMR223W)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B Gene:VAM3(YOR106W)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VFA1(YER128W)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:YCH1(YGR203W)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Phosphatase with sequence similarity to Cdc25p, Arr2p and Mih1p; member of the single-domain rhodanese homology superfamily; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YDR008C(YDR008C_d)|FD-Score:5.81|P-value:3.11E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJL068C(YJL068C)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D Gene:YJR142W(YJR142W_p)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member Gene:YLR126C(YLR126C_p)|FD-Score:5.17|P-value:1.17E-7||SGD DESC:Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:YLR271W(YLR271W_p)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR422W(YLR422W_p)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Protein of unknown function with similarity to human DOCK proteins (guanine nucleotide exchange factors); interacts with Ino4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, YLR422W is not an essential protein Gene:YNR066C(YNR066C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:YPL119C-A(YPL119C-A_p)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR397C5.131.48E-71.13NCB2Subunit of a heterodimeric NC2 transcription regulator complex with Bur6p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta
YKL108W4.003.23E-50.13SLD2Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
YMR211W3.875.55E-50.05DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YKR081C3.816.88E-50.13RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YLR208W3.681.16E-40.11SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YER133W3.571.80E-40.25GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YPR033C3.324.51E-40.05HTS1Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
YER159C3.275.31E-40.11BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YOL026C3.177.69E-40.00MIM1Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
YDR499W3.167.79E-40.02LCD1Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress
YBR088C3.158.28E-40.02POL30Proliferating cell nuclear antigen (PCNA), functions as the sliding clamp for DNA polymerase delta; may function as a docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair
YGL018C3.138.80E-40.11JAC1Specialized J-protein that functions with Hsp70 in Fe-S cluster biogenesis in mitochondria, involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix
YDL195W3.020.001270.13SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YDR037W2.890.001910.09KRS1Lysyl-tRNA synthetase
YDL164C2.800.002530.07CDC9DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C8.844.85E-19TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YER090W8.527.66E-18TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YIR023W6.863.44E-12DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YDR008C_d5.813.11E-9YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR126C_p5.171.17E-7YLR126C_pPutative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YNR031C5.042.30E-7SSK2MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress
YGL026C4.817.40E-7TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YDR127W4.651.67E-6ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YHL024W4.641.75E-6RIM4Putative RNA-binding protein required for the expression of early and middle sporulation genes
YPL219W4.523.14E-6PCL8Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation
YNL335W_p4.395.67E-6DDI3_pProtein of unknown function; expression is induced over 100-fold by DNA damage; induction decreased in rad6 and rad18 mutants
YOR106W4.261.00E-5VAM3Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region
YDR007W3.807.11E-5TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YMR205C3.631.41E-4PFK2Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
YMR202W3.621.45E-4ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis

GO enrichment analysis for SGTC_1710
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4129.64E-241SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.108108
0.3911.98E-214SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0416667
0.3896.46E-213SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.106667
0.3884.53E-211SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.276923
0.3801.40E-202SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.0810811
0.3693.38E-190SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0615385
0.3651.06E-185SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.157895
0.3537.16E-173SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)3015189NA
0.3502.95E-170SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.050505
0.3506.50E-170SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.102273
0.3492.14E-168SGTC_1824st053723 68.6 μMTimTec (Natural product derivative library)6752250.08
0.3492.67E-168SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.175676
0.3484.60E-168SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.0493827
0.3428.44E-162SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.1
0.3341.10E-153SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_14404100-155040 μM0.534483742806ChemDiv (Drug-like library)268.267382.23914
SGTC_7991310-0557151 μM0.5283025354492ChemDiv (Drug-like library)252.264683.4703superoxide
SGTC_23157733312200 μM0.462963726316Chembridge (Fragment library)210.25291.93523
SGTC_5544483-2211187 μM0.438596747107ChemDiv (Drug-like library)285.29462.45314
SGTC_2587piperic acid92.2 μM0.4285715370536Microsource (Natural product library)218.20542.16314
SGTC_23137726885200 μM0.42623726423Chembridge (Fragment library)293.384562.8614RPP1 & pyrimidine depletion
SGTC_3163909991449.47 μM0.4262316268368Chembridge (Drug-like library)260.245381.49815
SGTC_3232913287449.47 μM0.4181823784940Chembridge (Drug-like library)255.268622.4713RPP1 & pyrimidine depletion
SGTC_1795st04954562.3 μM0.4166675291882TimTec (Natural product derivative library)321.15484.79803TSC3-RPN4
SGTC_2897904859812.99 μM0.4098366467404Chembridge (Drug-like library)283.321782.99113