st033146

3-bromo-4-[(4-fluorophenyl)methoxy]-5-methoxybenzaldehyde

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1713
Screen concentration 12.8 μM
Source TimTec (Natural product derivative library)
PubChem CID 674619
SMILES COC1=C(C(=CC(=C1)C=O)Br)OCC2=CC=C(C=C2)F
Standardized SMILES COc1cc(C=O)cc(Br)c1OCc2ccc(F)cc2
Molecular weight 339.1564
ALogP 4.09
H-bond donor count 0
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.27
% growth inhibition (Hom. pool) 6.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 674619
Download HIP data (tab-delimited text)  (excel)
Gene:BIG1(YHR101C)|FD-Score:-3.82|P-value:6.80E-5|Clearance:0||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:CDC13(YDL220C)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.09||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CDC15(YAR019C)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.07||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:DIS3(YOL021C)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.26||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:GAA1(YLR088W)|FD-Score:3.82|P-value:6.64E-5|Clearance:0.22||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:NOP2(YNL061W)|FD-Score:5.54|P-value:1.49E-8|Clearance:0.37||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP8(YOL144W)|FD-Score:-3.17|P-value:7.71E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PDI1(YCL043C)|FD-Score:4.09|P-value:2.16E-5|Clearance:0.02||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:POL5(YEL055C)|FD-Score:5.21|P-value:9.51E-8|Clearance:0.37||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:PRE5(YMR314W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.13||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PTR3(YFR029W)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.07||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:PZF1(YPR186C)|FD-Score:4.74|P-value:1.04E-6|Clearance:0.37||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:ROT1(YMR200W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPF1(YHR088W)|FD-Score:5.84|P-value:2.62E-9|Clearance:0.37||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:SDO1(YLR022C)|FD-Score:4.99|P-value:3.08E-7|Clearance:0.37||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SEC16(YPL085W)|FD-Score:-3.94|P-value:4.06E-5|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SRP1(YNL189W)|FD-Score:4.37|P-value:6.18E-6|Clearance:0.26||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TIF5(YPR041W)|FD-Score:-3.5|P-value:2.30E-4|Clearance:0||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:TRR1(YDR353W)|FD-Score:4.07|P-value:2.35E-5|Clearance:0.14||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:TUB2(YFL037W)|FD-Score:4.11|P-value:1.97E-5|Clearance:0.02||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YAH1(YPL252C)|FD-Score:3.93|P-value:4.31E-5|Clearance:0.11||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin Gene:BIG1(YHR101C)|FD-Score:-3.82|P-value:6.80E-5|Clearance:0||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:CDC13(YDL220C)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.09||SGD DESC:Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation Gene:CDC15(YAR019C)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.07||SGD DESC:Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress Gene:DIS3(YOL021C)|FD-Score:3.25|P-value:5.77E-4|Clearance:0.26||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:GAA1(YLR088W)|FD-Score:3.82|P-value:6.64E-5|Clearance:0.22||SGD DESC:Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER Gene:NOP2(YNL061W)|FD-Score:5.54|P-value:1.49E-8|Clearance:0.37||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP8(YOL144W)|FD-Score:-3.17|P-value:7.71E-4|Clearance:0||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PDI1(YCL043C)|FD-Score:4.09|P-value:2.16E-5|Clearance:0.02||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:POL5(YEL055C)|FD-Score:5.21|P-value:9.51E-8|Clearance:0.37||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:PRE5(YMR314W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.13||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PTR3(YFR029W)|FD-Score:3.41|P-value:3.30E-4|Clearance:0.07||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:PZF1(YPR186C)|FD-Score:4.74|P-value:1.04E-6|Clearance:0.37||SGD DESC:Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA Gene:ROT1(YMR200W)|FD-Score:-3.62|P-value:1.47E-4|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPF1(YHR088W)|FD-Score:5.84|P-value:2.62E-9|Clearance:0.37||SGD DESC:Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA Gene:SDO1(YLR022C)|FD-Score:4.99|P-value:3.08E-7|Clearance:0.37||SGD DESC:Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes Gene:SEC16(YPL085W)|FD-Score:-3.94|P-value:4.06E-5|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SRP1(YNL189W)|FD-Score:4.37|P-value:6.18E-6|Clearance:0.26||SGD DESC:Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation Gene:TIF5(YPR041W)|FD-Score:-3.5|P-value:2.30E-4|Clearance:0||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:TRR1(YDR353W)|FD-Score:4.07|P-value:2.35E-5|Clearance:0.14||SGD DESC:Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication Gene:TUB2(YFL037W)|FD-Score:4.11|P-value:1.97E-5|Clearance:0.02||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YAH1(YPL252C)|FD-Score:3.93|P-value:4.31E-5|Clearance:0.11||SGD DESC:Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 674619
Download HOP data (tab-delimited text)  (excel)
Gene:AAD6(YFL056C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADE12(YNL220W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AIM21(YIR003W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:ALD5(YER073W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:ALG9(YNL219C)|FD-Score:4.89|P-value:4.95E-7||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:ANT1(YPR128C)|FD-Score:5.97|P-value:1.19E-9||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:APN1(YKL114C)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARR3(YPR201W)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ATG7(YHR171W)|FD-Score:-4.57|P-value:2.45E-6||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP23(YNR020C)|FD-Score:4.32|P-value:7.63E-6||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:BNA3(YJL060W)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BUD23(YCR047C)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CAN1(YEL063C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:COA4(YLR218C)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:CRP1(YHR146W)|FD-Score:-4.07|P-value:2.31E-5||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTK3(YML112W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:-3.8|P-value:7.27E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL7(YIR031C)|FD-Score:-6.96|P-value:1.67E-12||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DCV1(YFR012W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DSE2(YHR143W)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DUS3(YLR401C)|FD-Score:5.14|P-value:1.38E-7||SGD DESC:Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress Gene:FAA2(YER015W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FAR8(YMR029C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:GIP3(YPL137C)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GLG2(YJL137C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GLR1(YPL091W)|FD-Score:5.97|P-value:1.17E-9||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GTO3(YMR251W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HAC1(YFL031W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HAL9(YOL089C)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HKR1(YDR420W)|FD-Score:4.44|P-value:4.60E-6||SGD DESC:Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection Gene:ILV1(YER086W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:ISC10(YER180C)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KTI11(YBL071W-A)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MCM16(YPR046W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDM12(YOL009C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MEP3(YPR138C)|FD-Score:5.23|P-value:8.37E-8||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MID2(YLR332W)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MNL2(YLR057W)|FD-Score:6.94|P-value:1.90E-12||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRP51(YPL118W)|FD-Score:8.26|P-value:7.00E-17||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MUD2(YKL074C)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 Gene:PBP1(YGR178C)|FD-Score:-5.08|P-value:1.93E-7||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PCA1(YBR295W)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PEX2(YJL210W)|FD-Score:3.81|P-value:6.81E-5||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PML39(YML107C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:POT1(YIL160C)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPM1(YDR435C)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PUF2(YPR042C)|FD-Score:4.91|P-value:4.67E-7||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:QCR9(YGR183C)|FD-Score:-9.45|P-value:1.62E-21||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:REC102(YLR329W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:RPL18B(YNL301C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RTS3(YGR161C_p)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SKN7(YHR206W)|FD-Score:-4.08|P-value:2.21E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SRV2(YNL138W)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:TDA6(YPR157W_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TDH1(YJL052W)|FD-Score:4.77|P-value:9.24E-7||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:THI4(YGR144W)|FD-Score:-4.67|P-value:1.54E-6||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TPS2(YDR074W)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TVP23(YDR084C)|FD-Score:-4.15|P-value:1.70E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TYW3(YGL050W)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UBP11(YKR098C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:UGA2(YBR006W)|FD-Score:4.82|P-value:7.20E-7||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS20(YMR077C)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS25(YJR102C)|FD-Score:-3.72|P-value:9.89E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS27(YNR006W)|FD-Score:-3.73|P-value:9.44E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS33(YLR396C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:XRS2(YDR369C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBL010C(YBL010C_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR012C(YBR012C_d)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR174C(YBR174C_d)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCK3(YER123W)|FD-Score:3.8|P-value:7.13E-5||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR417C(YDR417C_d)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YER134C(YER134C)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YGR169C-A(YGR169C-A_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YIL102C(YIL102C_p)|FD-Score:-4.97|P-value:3.43E-7||SGD DESC:Putative protein of unknown function Gene:YJL047C-A(YJL047C-A_p)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Putative protein of unknown function Gene:YKR015C(YKR015C_p)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function Gene:YKR073C(YKR073C_d)|FD-Score:-3.85|P-value:5.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR112W(YLR112W_d)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR123C(YLR123C_d)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif Gene:YLR311C(YLR311C_d)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR434C(YLR434C_d)|FD-Score:5.11|P-value:1.65E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YMR082C(YMR082C_d)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function Gene:YMR253C(YMR253C_p)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YMR306C-A(YMR306C-A_d)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR315W(YMR315W)|FD-Score:4.36|P-value:6.38E-6||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YNL276C(YNL276C_d)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YNR014W(YNR014W_p)|FD-Score:-5.43|P-value:2.83E-8||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YNR066C(YNR066C_p)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOL013W-B(YOL013W-B_d)|FD-Score:-7.61|P-value:1.38E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Gene:YOL014W(YOL014W_p)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function Gene:YOL079W(YOL079W_d)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOP1(YPR028W)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR263C(YOR263C_d)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YOR379C(YOR379C_d)|FD-Score:5.35|P-value:4.52E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:YPL168W(YPL168W_p)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPR170C(YPR170C_d)|FD-Score:4.32|P-value:7.82E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPT52(YKR014C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:AAD6(YFL056C)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin Gene:ADE12(YNL220W)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence Gene:AIM21(YIR003W)|FD-Score:3.2|P-value:6.91E-4||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:ALD5(YER073W)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:ALG9(YNL219C)|FD-Score:4.89|P-value:4.95E-7||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:ANT1(YPR128C)|FD-Score:5.97|P-value:1.19E-9||SGD DESC:Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation Gene:APN1(YKL114C)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:Major apurinic/apyrimidinic endonuclease, 3'-repair diesterase involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine Gene:ARR3(YPR201W)|FD-Score:4.53|P-value:2.95E-6||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:ATG7(YHR171W)|FD-Score:-4.57|P-value:2.45E-6||SGD DESC:Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation Gene:ATP23(YNR020C)|FD-Score:4.32|P-value:7.63E-6||SGD DESC:Putative metalloprotease of the mitochondrial inner membrane, required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p Gene:BNA3(YJL060W)|FD-Score:4.14|P-value:1.76E-5||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BUD23(YCR047C)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:CAN1(YEL063C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication Gene:COA4(YLR218C)|FD-Score:-3.64|P-value:1.39E-4||SGD DESC:Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors Gene:CRP1(YHR146W)|FD-Score:-4.07|P-value:2.31E-5||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTK3(YML112W)|FD-Score:-3.12|P-value:8.97E-4||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CTR1(YPR124W)|FD-Score:-3.8|P-value:7.27E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL7(YIR031C)|FD-Score:-6.96|P-value:1.67E-12||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DCV1(YFR012W)|FD-Score:3.11|P-value:9.50E-4||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DSE2(YHR143W)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:DUS3(YLR401C)|FD-Score:5.14|P-value:1.38E-7||SGD DESC:Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress Gene:FAA2(YER015W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FAR8(YMR029C)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p Gene:GIP3(YPL137C)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:GLG2(YJL137C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GLR1(YPL091W)|FD-Score:5.97|P-value:1.17E-9||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GTO3(YMR251W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HAC1(YFL031W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress Gene:HAL9(YOL089C)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HKR1(YDR420W)|FD-Score:4.44|P-value:4.60E-6||SGD DESC:Mucin family member that functions as an osmosensor in the Sho1p-mediated HOG pathway with Msb2p; proposed to be a negative regulator of filamentous growth; mutant displays defects in beta-1,3 glucan synthesis and bud site selection Gene:ILV1(YER086W)|FD-Score:-3.55|P-value:1.95E-4||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:ISC10(YER180C)|FD-Score:3.88|P-value:5.29E-5||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KTI11(YBL071W-A)|FD-Score:3.15|P-value:8.09E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MCM16(YPR046W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MDM12(YOL009C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MEP3(YPR138C)|FD-Score:5.23|P-value:8.37E-8||SGD DESC:Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease Gene:MID2(YLR332W)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MNL2(YLR057W)|FD-Score:6.94|P-value:1.90E-12||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MRP51(YPL118W)|FD-Score:8.26|P-value:7.00E-17||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MUD2(YKL074C)|FD-Score:5.82|P-value:2.91E-9||SGD DESC:Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65 Gene:PBP1(YGR178C)|FD-Score:-5.08|P-value:1.93E-7||SGD DESC:Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:PCA1(YBR295W)|FD-Score:4.57|P-value:2.44E-6||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PEX2(YJL210W)|FD-Score:3.81|P-value:6.81E-5||SGD DESC:RING-finger peroxin and E3 ubiquitin ligase, peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import Gene:PML39(YML107C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Protein required for nuclear retention of unspliced pre-mRNAs along with Mlp1p and Pml1p; anchored to nuclear pore complex via Mlp1p and Mlp2p; found with the subset of nuclear pores farthest from the nucleolus; may interact with ribosomes Gene:POT1(YIL160C)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PPM1(YDR435C)|FD-Score:3.13|P-value:8.72E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PUF2(YPR042C)|FD-Score:4.91|P-value:4.67E-7||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins Gene:QCR9(YGR183C)|FD-Score:-9.45|P-value:1.62E-21||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:REC102(YLR329W)|FD-Score:-3.59|P-value:1.63E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination Gene:RPL18B(YNL301C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication Gene:RPL6A(YML073C)|FD-Score:-3.27|P-value:5.30E-4||SGD DESC:Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RTS3(YGR161C_p)|FD-Score:-3.56|P-value:1.82E-4||SGD DESC:Putative component of the protein phosphatase type 2A complex Gene:SKN7(YHR206W)|FD-Score:-4.08|P-value:2.21E-5||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SRV2(YNL138W)|FD-Score:3.77|P-value:8.32E-5||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:TDA6(YPR157W_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TDH1(YJL052W)|FD-Score:4.77|P-value:9.24E-7||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:THI4(YGR144W)|FD-Score:-4.67|P-value:1.54E-6||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TPS2(YDR074W)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress Gene:TVP23(YDR084C)|FD-Score:-4.15|P-value:1.70E-5||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:TYW3(YGL050W)|FD-Score:4.56|P-value:2.60E-6||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UBP11(YKR098C)|FD-Score:-3.72|P-value:1.01E-4||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins Gene:UGA2(YBR006W)|FD-Score:4.82|P-value:7.20E-7||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VPS20(YMR077C)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS25(YJR102C)|FD-Score:-3.72|P-value:9.89E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS27(YNR006W)|FD-Score:-3.73|P-value:9.44E-5||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS33(YLR396C)|FD-Score:3.22|P-value:6.35E-4||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:XRS2(YDR369C)|FD-Score:3.6|P-value:1.62E-4||SGD DESC:Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling Gene:YBL010C(YBL010C_p)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YBL100W-C(YBL100W-C_p)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YBR012C(YBR012C_d)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YBR174C(YBR174C_d)|FD-Score:3.29|P-value:5.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective Gene:YCK3(YER123W)|FD-Score:3.8|P-value:7.13E-5||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR417C(YDR417C_d)|FD-Score:3.81|P-value:7.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YER134C(YER134C)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YGR169C-A(YGR169C-A_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YIL102C(YIL102C_p)|FD-Score:-4.97|P-value:3.43E-7||SGD DESC:Putative protein of unknown function Gene:YJL047C-A(YJL047C-A_p)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Putative protein of unknown function Gene:YKR015C(YKR015C_p)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function Gene:YKR073C(YKR073C_d)|FD-Score:-3.85|P-value:5.89E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR112W(YLR112W_d)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR123C(YLR123C_d)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif Gene:YLR311C(YLR311C_d)|FD-Score:4.05|P-value:2.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR434C(YLR434C_d)|FD-Score:5.11|P-value:1.65E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W Gene:YMR082C(YMR082C_d)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR247W-A(YMR247W-A_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function Gene:YMR253C(YMR253C_p)|FD-Score:3.48|P-value:2.48E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene Gene:YMR306C-A(YMR306C-A_d)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR315W(YMR315W)|FD-Score:4.36|P-value:6.38E-6||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YNL276C(YNL276C_d)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W Gene:YNR014W(YNR014W_p)|FD-Score:-5.43|P-value:2.83E-8||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YNR066C(YNR066C_p)|FD-Score:4.04|P-value:2.72E-5||SGD DESC:Putative membrane-localized protein of unknown function Gene:YOL013W-B(YOL013W-B_d)|FD-Score:-7.61|P-value:1.38E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Gene:YOL014W(YOL014W_p)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function Gene:YOL079W(YOL079W_d)|FD-Score:-3.48|P-value:2.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOP1(YPR028W)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Membrane protein that interacts with Yip1p to mediate membrane traffic; interacts with Sey1p to maintain ER morphology; overexpression leads to cell death and accumulation of internal cell membranes; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; forms ER foci upon DNA replication stress Gene:YOR263C(YOR263C_d)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:YOR379C(YOR379C_d)|FD-Score:5.35|P-value:4.52E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:YPL168W(YPL168W_p)|FD-Score:3.3|P-value:4.80E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YPR170C(YPR170C_d)|FD-Score:4.32|P-value:7.82E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YPT52(YKR014C)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR088W5.842.62E-90.37RPF1Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA
YNL061W5.541.49E-80.37NOP2Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
YEL055C5.219.51E-80.37POL5DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
YLR022C4.993.08E-70.37SDO1Essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
YPR186C4.741.04E-60.37PZF1Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
YNL189W4.376.18E-60.26SRP1Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation
YFL037W4.111.97E-50.02TUB2Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
YCL043C4.092.16E-50.02PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YDR353W4.072.35E-50.14TRR1Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
YPL252C3.934.31E-50.10YAH1Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
YLR088W3.826.64E-50.23GAA1Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
YAR019C3.601.61E-40.07CDC15Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress
YMR314W3.532.07E-40.12PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YFR029W3.413.30E-40.07PTR3Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YDL220C3.344.18E-40.09CDC13Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL118W8.267.00E-17MRP51Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
YLR057W6.941.90E-12MNL2Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
YPL091W5.971.17E-9GLR1Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
YPR128C5.971.19E-9ANT1Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation
YKL074C5.822.91E-9MUD2Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
YOR379C_d5.354.52E-8YOR379C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W
YPR138C5.238.37E-8MEP3Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease
YLR401C5.141.38E-7DUS3Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
YLR434C_d5.111.65E-7YLR434C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W
YPR042C4.914.67E-7PUF2Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins
YNL219C4.894.95E-7ALG9Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
YBR006W4.827.20E-7UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YJL052W4.779.24E-7TDH1Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
YBR295W4.572.44E-6PCA1Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function
YGL050W4.562.60E-6TYW3tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions

GO enrichment analysis for SGTC_1713
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1048.79E-16SGTC_1716st033144 30.6 μMTimTec (Natural product derivative library)6746170.604167
0.0552.68E-5SGTC_1715st032160 10.2 μMTimTec (Natural product derivative library)52898250.0465116
0.0543.56E-5SGTC_1074carmofur 3.8 μMNIH Clinical Collection25770.1exosome
0.0543.84E-5SGTC_23459052315 157.4 μMChembridge (Fragment library)64692650.0923077
0.0534.37E-5SGTC_32679137951 49.5 μMChembridge (Drug-like library)166439860.126761
0.0527.49E-5SGTC_2703st077852 53.4 μMTimTec (Natural product derivative library)62818360.135802
0.0501.19E-4SGTC_1717st033143 58.0 μMTimTec (Natural product derivative library)6746160.436364
0.0482.08E-4SGTC_23339017922 200.0 μMChembridge (Fragment library)31631640.0666667
0.0472.65E-4SGTC_1714st033236 13.6 μMTimTec (Natural product derivative library)29165660.653061
0.0464.27E-4SGTC_14544239-0054 42.6 μMChemDiv (Drug-like library)13407670.2
0.0439.20E-4SGTC_16985-fluorocytosine 1.1 μMTimTec (Natural product derivative library)33660.0892857exosome
0.0420.00112SGTC_14534237-0541 195.0 μMChemDiv (Drug-like library)7398920.0684932heme biosynthesis & mitochondrial translocase
0.0420.00118SGTC_6934161-2696 114.0 μMChemDiv (Drug-like library)8057590.245902
0.0420.00121SGTC_1505k297-0037 20.6 μMChemDiv (Drug-like library)40893960.0857143
0.0420.00126SGTC_2236dapsone 200.0 μMMiscellaneous29550.0784314

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1708st03323431.83 μM0.767442674664TimTec (Natural product derivative library)321.165923.88803
SGTC_1709st03317939.1 μM0.666667573381TimTec (Natural product derivative library)339.1563834.09304
SGTC_1714st03323613.63 μM0.6530612916566TimTec (Natural product derivative library)355.610984.55203
SGTC_1716st03314430.62 μM0.604167674617TimTec (Natural product derivative library)335.19254.23703
SGTC_1631benzylvanillin81.9 μM0.46938875506TimTec (Natural product derivative library)242.269863.1403Golgi
SGTC_1717st03314358.04 μM0.436364674616TimTec (Natural product derivative library)294.7053834.0104
SGTC_5883970-114452.9 μM0.3666671888707ChemDiv (Drug-like library)425.2609434.86425
SGTC_1633st00566467.9 μM0.3653852063822TimTec (Natural product derivative library)208.253642.88503
SGTC_431k089-0097107 μM0.3611115499420ChemDiv (Drug-like library)521.745124.9247iron homeostasis
SGTC_1632st00565090 μM0.3584912759605TimTec (Natural product derivative library)222.280223.34103