st032160

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1715
Screen concentration 10.2 μM
Source TimTec (Natural product derivative library)
PubChem CID 5289825
SMILES CC(=O)C12C(N1C(=O)C)CC3C2(CCC4C3CC=C5C4(CCC(C5)O)C)C
Standardized SMILES CC(=O)N1C2CC3C4CC=C5CC(O)CCC5(C)C4CCC3(C)C12C(=O)C
Molecular weight 371.513
ALogP 2.22
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.12
% growth inhibition (Hom. pool) 11.36


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5289825
Download HIP data (tab-delimited text)  (excel)
Gene:CAK1(YFL029C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.07||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCT3(YJL014W)|FD-Score:-4.19|P-value:1.42E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:-4.08|P-value:2.27E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDS1(YBR029C)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.13||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CSE1(YGL238W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.08||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:DPM1(YPR183W)|FD-Score:-3.53|P-value:2.11E-4|Clearance:0||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:EPL1(YFL024C)|FD-Score:3.82|P-value:6.56E-5|Clearance:0.08||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:FMN1(YDR236C)|FD-Score:3.73|P-value:9.53E-5|Clearance:0.09||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GLC7(YER133W)|FD-Score:-3.92|P-value:4.49E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.13||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:MED4(YOR174W)|FD-Score:3.75|P-value:8.95E-5|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MTR10(YOR160W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.01||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MTR3(YGR158C)|FD-Score:-3.75|P-value:8.73E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOP15(YNL110C)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PRE6(YOL038W)|FD-Score:3.29|P-value:4.95E-4|Clearance:0.08||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RAD3(YER171W)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.06||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RIB5(YBR256C)|FD-Score:3.99|P-value:3.32E-5|Clearance:0.16||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPP1(YHR062C)|FD-Score:-4.01|P-value:3.01E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SCL1(YGL011C)|FD-Score:4.53|P-value:2.90E-6|Clearance:0.34||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SMP3(YOR149C)|FD-Score:3.1|P-value:9.54E-4|Clearance:0.03||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SOF1(YLL011W)|FD-Score:5.45|P-value:2.58E-8|Clearance:0.91||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:TFC1(YBR123C)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.11||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TIF5(YPR041W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.08||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:YPL238C(YPL238C_d)|FD-Score:-4.23|P-value:1.14E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W Gene:CAK1(YFL029C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.07||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CCT3(YJL014W)|FD-Score:-4.19|P-value:1.42E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:-4.08|P-value:2.27E-5|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CDS1(YBR029C)|FD-Score:3.64|P-value:1.36E-4|Clearance:0.13||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:CSE1(YGL238W)|FD-Score:4.07|P-value:2.34E-5|Clearance:0.08||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:DPM1(YPR183W)|FD-Score:-3.53|P-value:2.11E-4|Clearance:0||SGD DESC:Dolichol phosphate mannose (Dol-P-Man) synthase of the ER membrane, catalyzes the formation of Dol-P-Man from Dol-P and GDP-Man; required for glycosyl phosphatidylinositol membrane anchoring, O mannosylation, and protein glycosylation Gene:EPL1(YFL024C)|FD-Score:3.82|P-value:6.56E-5|Clearance:0.08||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:FMN1(YDR236C)|FD-Score:3.73|P-value:9.53E-5|Clearance:0.09||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GLC7(YER133W)|FD-Score:-3.92|P-value:4.49E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPI14(YJR013W)|FD-Score:4.2|P-value:1.36E-5|Clearance:0.13||SGD DESC:Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M Gene:MED4(YOR174W)|FD-Score:3.75|P-value:8.95E-5|Clearance:0.02||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MTR10(YOR160W)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.01||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MTR3(YGR158C)|FD-Score:-3.75|P-value:8.73E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6) Gene:NOP15(YNL110C)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:PRE6(YOL038W)|FD-Score:3.29|P-value:4.95E-4|Clearance:0.08||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RAD3(YER171W)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.06||SGD DESC:5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress Gene:RIB5(YBR256C)|FD-Score:3.99|P-value:3.32E-5|Clearance:0.16||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPP1(YHR062C)|FD-Score:-4.01|P-value:3.01E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SCL1(YGL011C)|FD-Score:4.53|P-value:2.90E-6|Clearance:0.34||SGD DESC:Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria Gene:SMP3(YOR149C)|FD-Score:3.1|P-value:9.54E-4|Clearance:0.03||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SOF1(YLL011W)|FD-Score:5.45|P-value:2.58E-8|Clearance:0.91||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:TFC1(YBR123C)|FD-Score:3.21|P-value:6.56E-4|Clearance:0.11||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TIF5(YPR041W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.08||SGD DESC:Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2 Gene:YPL238C(YPL238C_d)|FD-Score:-4.23|P-value:1.14E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5289825
Download HOP data (tab-delimited text)  (excel)
Gene:AFT2(YPL202C)|FD-Score:4.27|P-value:9.75E-6||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:ALT1(YLR089C)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:APL5(YPL195W)|FD-Score:-7.55|P-value:2.11E-14||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ARG82(YDR173C)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARL1(YBR164C)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:5.28|P-value:6.35E-8||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:5.46|P-value:2.33E-8||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:6.13|P-value:4.35E-10||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:AVL9(YLR114C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BCK1(YJL095W)|FD-Score:4.93|P-value:4.17E-7||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BPH1(YCR032W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:CDC50(YCR094W)|FD-Score:10.2|P-value:1.34E-24||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CDH1(YGL003C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CKA1(YIL035C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLC1(YGR167W)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG6(YNL041C)|FD-Score:-5.83|P-value:2.70E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-5.34|P-value:4.67E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK3(YML112W)|FD-Score:-7.16|P-value:4.17E-13||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CYB5(YNL111C)|FD-Score:-3.78|P-value:7.96E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DCS1(YLR270W)|FD-Score:-4.36|P-value:6.63E-6||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DIA1(YMR316W)|FD-Score:4.36|P-value:6.54E-6||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DLD2(YDL178W)|FD-Score:-3.75|P-value:8.78E-5||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DON1(YDR273W)|FD-Score:4.83|P-value:6.97E-7||SGD DESC:Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II Gene:DUS3(YLR401C)|FD-Score:5.26|P-value:7.13E-8||SGD DESC:Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress Gene:DYN3(YMR299C)|FD-Score:-3.26|P-value:5.67E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ECM33(YBR078W)|FD-Score:-4.53|P-value:2.95E-6||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ECT1(YGR007W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:ERG2(YMR202W)|FD-Score:4.44|P-value:4.55E-6||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:-5.74|P-value:4.72E-9||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERI1(YPL096C-A)|FD-Score:-5.43|P-value:2.88E-8||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FMP21(YBR269C_p)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:9.82|P-value:4.75E-23||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:7.29|P-value:1.58E-13||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:11.7|P-value:7.52E-32||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.08|P-value:1.90E-7||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GOS1(YHL031C)|FD-Score:-3.9|P-value:4.74E-5||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPB2(YAL056W)|FD-Score:4.34|P-value:7.11E-6||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GSC2(YGR032W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GUT2(YIL155C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HSL7(YBR133C)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HTL1(YCR020W-B)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:HUL4(YJR036C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:IME1(YJR094C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:INO2(YDR123C)|FD-Score:5.49|P-value:2.01E-8||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:IRA2(YOL081W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:-7.15|P-value:4.43E-13||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:-4.78|P-value:8.87E-7||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:KSS1(YGR040W)|FD-Score:4.28|P-value:9.21E-6||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:KTI11(YBL071W-A)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MAM33(YIL070C)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MBR1(YKL093W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants Gene:MEF2(YJL102W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MET31(YPL038W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MRP51(YPL118W)|FD-Score:5.93|P-value:1.52E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MSH1(YHR120W)|FD-Score:-4.37|P-value:6.34E-6||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSS116(YDR194C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MTC4(YBR255W)|FD-Score:-6.15|P-value:3.86E-10||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NIP100(YPL174C)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:OPI3(YJR073C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAC10(YGR078C)|FD-Score:-4.59|P-value:2.24E-6||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PET309(YLR067C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PHD1(YKL043W)|FD-Score:-4.81|P-value:7.62E-7||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:PHO88(YBR106W)|FD-Score:5.25|P-value:7.43E-8||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIL1(YGR086C)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PRM8(YGL053W)|FD-Score:7.66|P-value:9.01E-15||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:PTH1(YHR189W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:QCR10(YHR001W-A)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain Gene:RCY1(YJL204C)|FD-Score:9.25|P-value:1.14E-20||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGL1(YPL066W)|FD-Score:6.17|P-value:3.52E-10||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGP1(YDR137W)|FD-Score:-6.19|P-value:2.95E-10||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:ROM2(YLR371W)|FD-Score:-3.95|P-value:3.95E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL36A(YMR194W)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:-4.52|P-value:3.11E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM24(YDR175C)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC3(YHR087W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTF1(YGL244W)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTT102(YGR275W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAP30(YMR263W)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SHU2(YDR078C)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIP2(YGL208W)|FD-Score:6.03|P-value:8.11E-10||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SLG1(YOR008C)|FD-Score:-4.17|P-value:1.52E-5||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF2(YOR290C)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOP4(YJL192C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPT23(YKL020C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:STP1(YDR463W)|FD-Score:-5.67|P-value:7.33E-9||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA46(YOR091W)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRP1(YDR007W)|FD-Score:19.2|P-value:9.81E-83||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:10.7|P-value:8.16E-27||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.69|P-value:7.36E-15||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.17|P-value:3.79E-13||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:12.6|P-value:7.31E-37||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:-3.74|P-value:9.34E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TVP18(YMR071C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UFD2(YDL190C)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:URA10(YMR271C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:URA5(YML106W)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:URK1(YNR012W)|FD-Score:-4.19|P-value:1.36E-5||SGD DESC:Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP Gene:VMA1(YDL185W)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA7(YGR020C)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPH1(YOR270C)|FD-Score:-4.1|P-value:2.04E-5||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS1(YKR001C)|FD-Score:-6.8|P-value:5.17E-12||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS3(YDR495C)|FD-Score:-4.08|P-value:2.28E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS61(YDR136C_d)|FD-Score:-6.94|P-value:2.03E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-5.18|P-value:1.13E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAP3(YHL009C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBR116C(YBR116C_d)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.99|P-value:3.27E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR238C(YBR238C)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCR007C(YCR007C_p)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:4.54|P-value:2.79E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:6.92|P-value:2.19E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR222W(YDR222W_p)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR509W(YDR509W_d)|FD-Score:-3.23|P-value:6.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER038W-A(YER038W-A_d)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER175W-A(YER175W-A_p)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YFL013W-A(YFL013W-A_d)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YGL082W(YGL082W_p)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGL117W(YGL117W_p)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Putative protein of unknown function Gene:YGR045C(YGR045C_d)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR069W(YGR069W_d)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR283C(YGR283C)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YJL064W(YJL064W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YML119W(YML119W_p)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YML122C(YML122C_d)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR306C-A(YMR306C-A_d)|FD-Score:5.51|P-value:1.79E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL143C(YNL143C_p)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL013W-B(YOL013W-B_d)|FD-Score:-5.35|P-value:4.35E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Gene:YOR015W(YOR015W_d)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR379C(YOR379C_d)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:YPK1(YKL126W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL039W(YPL039W_p)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPR064W(YPR064W_d)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR092W(YPR092W_d)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPS7(YDR349C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YPT52(YKR014C)|FD-Score:9.46|P-value:1.55E-21||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:YPT6(YLR262C)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:AFT2(YPL202C)|FD-Score:4.27|P-value:9.75E-6||SGD DESC:Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication Gene:ALT1(YLR089C)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive Gene:APL5(YPL195W)|FD-Score:-7.55|P-value:2.11E-14||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:ARG82(YDR173C)|FD-Score:-3.42|P-value:3.12E-4||SGD DESC:Inositol polyphosphate multikinase (IPMK), sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates arginine-, phosphate-, and nitrogen-responsive genes Gene:ARL1(YBR164C)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor Gene:ARO1(YDR127W)|FD-Score:5.28|P-value:6.35E-8||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:5.46|P-value:2.33E-8||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:6.13|P-value:4.35E-10||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:AVL9(YLR114C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BCK1(YJL095W)|FD-Score:4.93|P-value:4.17E-7||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BPH1(YCR032W)|FD-Score:3.39|P-value:3.49E-4||SGD DESC:Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting Gene:CDC50(YCR094W)|FD-Score:10.2|P-value:1.34E-24||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CDH1(YGL003C)|FD-Score:4.41|P-value:5.15E-6||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:CKA1(YIL035C)|FD-Score:3.29|P-value:4.96E-4||SGD DESC:Alpha catalytic subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching Gene:CLC1(YGR167W)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG6(YNL041C)|FD-Score:-5.83|P-value:2.70E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:-5.34|P-value:4.67E-8||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTK3(YML112W)|FD-Score:-7.16|P-value:4.17E-13||SGD DESC:Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress Gene:CYB5(YNL111C)|FD-Score:-3.78|P-value:7.96E-5||SGD DESC:Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation Gene:DCS1(YLR270W)|FD-Score:-4.36|P-value:6.63E-6||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DIA1(YMR316W)|FD-Score:4.36|P-value:6.54E-6||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DLD2(YDL178W)|FD-Score:-3.75|P-value:8.78E-5||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:DON1(YDR273W)|FD-Score:4.83|P-value:6.97E-7||SGD DESC:Meiosis-specific component of the spindle pole body, part of the leading edge protein (LEP) coat, forms a ring-like structure at the leading edge of the prospore membrane during meiosis II Gene:DUS3(YLR401C)|FD-Score:5.26|P-value:7.13E-8||SGD DESC:Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress Gene:DYN3(YMR299C)|FD-Score:-3.26|P-value:5.67E-4||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ECM33(YBR078W)|FD-Score:-4.53|P-value:2.95E-6||SGD DESC:GPI-anchored protein of unknown function, has a possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p and Pst1p Gene:ECT1(YGR007W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:ERG2(YMR202W)|FD-Score:4.44|P-value:4.55E-6||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:-5.74|P-value:4.72E-9||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:ERI1(YPL096C-A)|FD-Score:-5.43|P-value:2.88E-8||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FMP21(YBR269C_p)|FD-Score:3.29|P-value:4.97E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:9.82|P-value:4.75E-23||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN20(YFR009W)|FD-Score:7.29|P-value:1.58E-13||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GCN3(YKR026C)|FD-Score:11.7|P-value:7.52E-32||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:5.08|P-value:1.90E-7||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GOS1(YHL031C)|FD-Score:-3.9|P-value:4.74E-5||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPB2(YAL056W)|FD-Score:4.34|P-value:7.11E-6||SGD DESC:Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication Gene:GSC2(YGR032W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GUT2(YIL155C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner Gene:HSL7(YBR133C)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p; relocalizes away from bud neck upon DNA replication stress Gene:HTL1(YCR020W-B)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:HUL4(YJR036C)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex Gene:IME1(YJR094C)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:INO2(YDR123C)|FD-Score:5.49|P-value:2.01E-8||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:IRA2(YOL081W)|FD-Score:3.58|P-value:1.75E-4||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:-7.15|P-value:4.43E-13||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISA1(YLL027W)|FD-Score:-4.78|P-value:8.87E-7||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins Gene:KSS1(YGR040W)|FD-Score:4.28|P-value:9.21E-6||SGD DESC:Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains Gene:KTI11(YBL071W-A)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs Gene:MAM33(YIL070C)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MBR1(YKL093W)|FD-Score:4.66|P-value:1.57E-6||SGD DESC:Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants Gene:MEF2(YJL102W)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MET31(YPL038W)|FD-Score:-3.71|P-value:1.03E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET31 has a paralog, MET32, that arose from the whole genome duplication Gene:MRP51(YPL118W)|FD-Score:5.93|P-value:1.52E-9||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MSH1(YHR120W)|FD-Score:-4.37|P-value:6.34E-6||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MSS116(YDR194C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing Gene:MTC4(YBR255W)|FD-Score:-6.15|P-value:3.86E-10||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NIP100(YPL174C)|FD-Score:-3.53|P-value:2.11E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:OPI3(YJR073C)|FD-Score:3.29|P-value:5.01E-4||SGD DESC:Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis Gene:PAC10(YGR078C)|FD-Score:-4.59|P-value:2.24E-6||SGD DESC:Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding Gene:PET309(YLR067C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs) Gene:PHD1(YKL043W)|FD-Score:-4.81|P-value:7.62E-7||SGD DESC:Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication Gene:PHO88(YBR106W)|FD-Score:5.25|P-value:7.43E-8||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PIL1(YGR086C)|FD-Score:3.88|P-value:5.27E-5||SGD DESC:Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:PRM8(YGL053W)|FD-Score:7.66|P-value:9.01E-15||SGD DESC:Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family Gene:PTH1(YHR189W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:QCR10(YHR001W-A)|FD-Score:3.12|P-value:9.14E-4||SGD DESC:Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain Gene:RCY1(YJL204C)|FD-Score:9.25|P-value:1.14E-20||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGL1(YPL066W)|FD-Score:6.17|P-value:3.52E-10||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RGP1(YDR137W)|FD-Score:-6.19|P-value:2.95E-10||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RIC1(YLR039C)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:ROM2(YLR371W)|FD-Score:-3.95|P-value:3.95E-5||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPL36A(YMR194W)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:-4.2|P-value:1.33E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RSM18(YER050C)|FD-Score:-4.52|P-value:3.11E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RSM24(YDR175C)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:RTC3(YHR087W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTF1(YGL244W)|FD-Score:-3.22|P-value:6.40E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTT102(YGR275W)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAP30(YMR263W)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SHU2(YDR078C)|FD-Score:4.62|P-value:1.94E-6||SGD DESC:Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function Gene:SIP2(YGL208W)|FD-Score:6.03|P-value:8.11E-10||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SLG1(YOR008C)|FD-Score:-4.17|P-value:1.52E-5||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SLT2(YHR030C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SNF2(YOR290C)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SOP4(YJL192C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SPT23(YKL020C)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:STP1(YDR463W)|FD-Score:-5.67|P-value:7.33E-9||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SYS1(YJL004C)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:TAT1(YBR069C)|FD-Score:-3.23|P-value:6.26E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TLG2(YOL018C)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA46(YOR091W)|FD-Score:3.85|P-value:5.86E-5||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRP1(YDR007W)|FD-Score:19.2|P-value:9.81E-83||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:10.7|P-value:8.16E-27||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:7.69|P-value:7.36E-15||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:7.17|P-value:3.79E-13||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:12.6|P-value:7.31E-37||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUF1(YOR187W)|FD-Score:-3.74|P-value:9.34E-5||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:TVP18(YMR071C)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UFD2(YDL190C)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3 Gene:URA10(YMR271C)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:URA5(YML106W)|FD-Score:3.89|P-value:4.94E-5||SGD DESC:Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication Gene:URK1(YNR012W)|FD-Score:-4.19|P-value:1.36E-5||SGD DESC:Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP Gene:VMA1(YDL185W)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMA7(YGR020C)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPH1(YOR270C)|FD-Score:-4.1|P-value:2.04E-5||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS1(YKR001C)|FD-Score:-6.8|P-value:5.17E-12||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS3(YDR495C)|FD-Score:-4.08|P-value:2.28E-5||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS61(YDR136C_d)|FD-Score:-6.94|P-value:2.03E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:VPS63(YLR261C_d)|FD-Score:-5.18|P-value:1.13E-7||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect Gene:YAP3(YHL009C)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor Gene:YBR116C(YBR116C_d)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2 Gene:YBR196C-A(YBR196C-A_p)|FD-Score:-3.99|P-value:3.27E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR238C(YBR238C)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCR007C(YCR007C_p)|FD-Score:-3.54|P-value:1.97E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:4.54|P-value:2.79E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:6.92|P-value:2.19E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR222W(YDR222W_p)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR509W(YDR509W_d)|FD-Score:-3.23|P-value:6.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER038W-A(YER038W-A_d)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER175W-A(YER175W-A_p)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YFL013W-A(YFL013W-A_d)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YGL082W(YGL082W_p)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene Gene:YGL117W(YGL117W_p)|FD-Score:4.25|P-value:1.08E-5||SGD DESC:Putative protein of unknown function Gene:YGR045C(YGR045C_d)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR069W(YGR069W_d)|FD-Score:3.16|P-value:7.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR283C(YGR283C)|FD-Score:-3.85|P-value:5.86E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YJL064W(YJL064W_d)|FD-Score:-3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YML119W(YML119W_p)|FD-Score:4.43|P-value:4.67E-6||SGD DESC:Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate Gene:YML122C(YML122C_d)|FD-Score:-3.4|P-value:3.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR306C-A(YMR306C-A_d)|FD-Score:5.51|P-value:1.79E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL143C(YNL143C_p)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOL013W-B(YOL013W-B_d)|FD-Score:-5.35|P-value:4.35E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Gene:YOR015W(YOR015W_d)|FD-Score:-3.35|P-value:4.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR379C(YOR379C_d)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W Gene:YPK1(YKL126W)|FD-Score:4.11|P-value:2.00E-5||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL039W(YPL039W_p)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPR064W(YPR064W_d)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR092W(YPR092W_d)|FD-Score:3.14|P-value:8.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPS7(YDR349C)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YPT52(YKR014C)|FD-Score:9.46|P-value:1.55E-21||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:YPT6(YLR262C)|FD-Score:-5.08|P-value:1.88E-7||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLL011W5.452.58E-80.91SOF1Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
YGL011C4.532.90E-60.34SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YJR013W4.201.36E-50.12GPI14Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
YGL238W4.072.34E-50.08CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YBR256C3.993.32E-50.17RIB5Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
YFL024C3.826.56E-50.08EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
YOR174W3.758.95E-50.02MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YDR236C3.739.53E-50.09FMN1Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane
YBR029C3.641.36E-40.13CDS1Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids
YPR041W3.512.21E-40.08TIF5Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2
YFL029C3.442.95E-40.07CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YER171W3.363.87E-40.06RAD35' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
YOR160W3.304.83E-40.01MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YOL038W3.294.95E-40.08PRE6Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
YBR123C3.216.56E-40.11TFC1One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR007W19.209.81E-83TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YGL026C12.607.31E-37TRP5Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
YKR026C11.707.52E-32GCN3Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
YER090W10.708.16E-27TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YCR094W10.201.34E-24CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YDR283C9.824.75E-23GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YKR014C9.461.55E-21YPT52Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress
YJL204C9.251.14E-20RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YKL211C7.697.36E-15TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YGL053W7.669.01E-15PRM8Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family
YFR009W7.291.58E-13GCN20Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
YDR354W7.173.79E-13TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR008C_d6.922.19E-12YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPL066W6.173.52E-10RGL1Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
YDR035W6.134.35E-10ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan

GO enrichment analysis for SGTC_1715
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4426.68E-281SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.105263
0.4411.82E-279SGTC_20384100038 31.0 μMChembridge (Fragment library)35968030.0958904plasma membrane duress
0.4224.00E-254SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.534483
0.4195.07E-250SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.0674157plasma membrane duress
0.4151.83E-244SGTC_1649st011737 51.3 μMTimTec (Natural product derivative library)17610350.0568182
0.4123.19E-241SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413020.289855
0.4113.17E-239SGTC_33089128332 17.6 μMChembridge (Drug-like library)173328050.0217391plasma membrane duress
0.3962.41E-221SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.122222
0.3921.21E-215SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.289855
0.3842.98E-206SGTC_18825615643 20.0 μMMiscellaneous22530750.0520833TRP & mitochondrial translation
0.3801.06E-202SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.534483
0.3764.86E-198SGTC_14274048-4900 550.0 nMChemDiv (Drug-like library)241325860.08
0.3757.18E-197SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.402597
0.3602.99E-180SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0645161
0.3562.00E-176SGTC_6770929-0063 136.0 μMChemDiv (Drug-like library)15395830.0493827

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1757st04162031.84 μM0.6393445289815TimTec (Natural product derivative library)329.476342.42223
SGTC_2650prasterone100 μM0.53448316759247Microsource (Natural product library)288.424423.33812
SGTC_2758dehydroepiandrosterone69.35 μM0.5344835881Miscellaneous288.424423.33812
SGTC_2074185-00104.49 μM0.4102563761753ChemDiv (Drug-like library)427.66245.12413endomembrane recycling
SGTC_2607solasodine47.35 μM0.4025976710642Microsource (Natural product library)413.635824.36723
SGTC_1609st00157446.4 μM0.3717953960012TimTec (Natural product derivative library)430.576923.5615
SGTC_1563conessine56.1 μM0.342466441082TimTec (Pure natural product library)356.58784.43402
SGTC_1572epiandrosterone68.9 μM0.289855441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.289855441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.2898552754130Microsource (Natural product library)290.44033.58812cell wall
SGTC_266formosanin C406 nM0.24770673597Miscellaneous1015.184780.311020sphingolipid biosynthesis & PDR1