1112-0046

1-(azepan-1-yl)-3-(3,6-dichlorocarbazol-9-yl)propan-2-ol

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_172
Screen concentration 2.6 μM
Source ChemDiv (Drug-like library)
PubChem CID 2802135
SMILES C1CCCN(CC1)CC(CN2C3=C(C=C(C=C3)Cl)C4=C2C=CC(=C4)Cl)O
Standardized SMILES OC(CN1CCCCCC1)Cn2c3ccc(Cl)cc3c4cc(Cl)ccc24
Molecular weight 391.3341
ALogP 5.86
H-bond donor count 1
H-bond acceptor count 2
Response signature NEO1

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.22
% growth inhibition (Hom. pool) 3.74


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2802135
Download HIP data (tab-delimited text)  (excel)
Gene:ATP16(YDL004W)|FD-Score:-3.12|P-value:9.11E-4|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:DUO1(YGL061C)|FD-Score:4.05|P-value:2.61E-5|Clearance:0.01||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERB1(YMR049C)|FD-Score:4.07|P-value:2.39E-5|Clearance:0.02||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:HAS1(YMR290C)|FD-Score:3.81|P-value:6.82E-5|Clearance:0.62||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:MAS1(YLR163C)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.04||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCD1(YDL003W)|FD-Score:4.65|P-value:1.66E-6|Clearance:0.4||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:NEO1(YIL048W)|FD-Score:69|P-value:0|Clearance:62.44||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PIK1(YNL267W)|FD-Score:5.46|P-value:2.33E-8|Clearance:0.63||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RPC31(YNL151C)|FD-Score:-3.12|P-value:8.94E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RPF2(YKR081C)|FD-Score:3.82|P-value:6.74E-5|Clearance:0||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPP1(YHR062C)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT3(YDR394W)|FD-Score:4.25|P-value:1.09E-5|Clearance:0.18||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RRS1(YOR294W)|FD-Score:-3.36|P-value:3.91E-4|Clearance:0||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RSC6(YCR052W)|FD-Score:6.56|P-value:2.78E-11|Clearance:1.09||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SPB1(YCL054W)|FD-Score:4.04|P-value:2.71E-5|Clearance:0.22||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SPC25(YER018C)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:TRS20(YBR254C)|FD-Score:-4.58|P-value:2.32E-6|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:UTP8(YGR128C)|FD-Score:3.15|P-value:8.03E-4|Clearance:0.09||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.83|P-value:6.72E-7|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:ATP16(YDL004W)|FD-Score:-3.12|P-value:9.11E-4|Clearance:0||SGD DESC:Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:DUO1(YGL061C)|FD-Score:4.05|P-value:2.61E-5|Clearance:0.01||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:ERB1(YMR049C)|FD-Score:4.07|P-value:2.39E-5|Clearance:0.02||SGD DESC:Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1 Gene:HAS1(YMR290C)|FD-Score:3.81|P-value:6.82E-5|Clearance:0.62||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:MAS1(YLR163C)|FD-Score:3.19|P-value:7.06E-4|Clearance:0.04||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MCD1(YDL003W)|FD-Score:4.65|P-value:1.66E-6|Clearance:0.4||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:NEO1(YIL048W)|FD-Score:69|P-value:0|Clearance:62.44||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:PIK1(YNL267W)|FD-Score:5.46|P-value:2.33E-8|Clearance:0.63||SGD DESC:Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton Gene:RPC31(YNL151C)|FD-Score:-3.12|P-value:8.94E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C31 Gene:RPF2(YKR081C)|FD-Score:3.82|P-value:6.74E-5|Clearance:0||SGD DESC:Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles Gene:RPP1(YHR062C)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT3(YDR394W)|FD-Score:4.25|P-value:1.09E-5|Clearance:0.18||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RRS1(YOR294W)|FD-Score:-3.36|P-value:3.91E-4|Clearance:0||SGD DESC:Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice Gene:RSC6(YCR052W)|FD-Score:6.56|P-value:2.78E-11|Clearance:1.09||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SPB1(YCL054W)|FD-Score:4.04|P-value:2.71E-5|Clearance:0.22||SGD DESC:AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants Gene:SPC25(YER018C)|FD-Score:-3.18|P-value:7.45E-4|Clearance:0||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering Gene:TRS20(YBR254C)|FD-Score:-4.58|P-value:2.32E-6|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder Gene:UTP8(YGR128C)|FD-Score:3.15|P-value:8.03E-4|Clearance:0.09||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.83|P-value:6.72E-7|Clearance:0.18||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2802135
Download HOP data (tab-delimited text)  (excel)
Gene:ADO1(YJR105W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM10(YER087W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:APL2(YKL135C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:BDF1(YLR399C)|FD-Score:6.6|P-value:2.07E-11||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BIO3(YNR058W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BTS1(YPL069C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBF1(YJR060W)|FD-Score:7.2|P-value:2.99E-13||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:12.4|P-value:2.32E-35||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKB1(YGL019W)|FD-Score:13|P-value:1.09E-38||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:6.08|P-value:5.92E-10||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:DCV1(YFR012W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DOA4(YDR069C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DRS2(YAL026C)|FD-Score:5.65|P-value:8.12E-9||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DST1(YGL043W)|FD-Score:8.22|P-value:1.00E-16||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:EEB1(YPL095C)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ERP2(YAL007C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:ERV14(YGL054C)|FD-Score:7.01|P-value:1.16E-12||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:GCN5(YGR252W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GYP1(YOR070C)|FD-Score:6.48|P-value:4.59E-11||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAP4(YKL109W)|FD-Score:-4.27|P-value:9.73E-6||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:IMP2'(YIL154C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISA2(YPR067W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:ISW1(YBR245C)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:LAA1(YJL207C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LDB16(YCL005W)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:MAM3(YOL060C)|FD-Score:3.84|P-value:6.21E-5||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MAP1(YLR244C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MEF1(YLR069C)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MRPL38(YKL170W)|FD-Score:-4.97|P-value:3.40E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:NFT1(YKR103W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:NMA111(YNL123W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NOP13(YNL175C)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NOP6(YDL213C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:NPR1(YNL183C)|FD-Score:4.77|P-value:9.15E-7||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NTA1(YJR062C)|FD-Score:-4.23|P-value:1.15E-5||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:ODC1(YPL134C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:PAF1(YBR279W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PEP8(YJL053W)|FD-Score:6.42|P-value:6.83E-11||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PEX1(YKL197C)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PEX30(YLR324W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PEX32(YBR168W)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PIM1(YBL022C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PRS3(YHL011C)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTC1(YDL006W)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RAM1(YDL090C)|FD-Score:7.19|P-value:3.30E-13||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCY1(YJL204C)|FD-Score:23.8|P-value:7.33E-126||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGL1(YPL066W)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RNQ1(YCL028W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RPP1B(YDL130W)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RRT2(YBR246W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RSM18(YER050C)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTT101(YJL047C)|FD-Score:4.8|P-value:8.12E-7||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RXT3(YDL076C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:5.45|P-value:2.53E-8||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAF1(YBR280C)|FD-Score:3.77|P-value:8.03E-5||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SHR5(YOL110W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SKY1(YMR216C)|FD-Score:-3.91|P-value:4.61E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNC2(YOR327C)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SNF7(YLR025W)|FD-Score:4.96|P-value:3.56E-7||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPS2(YDR522C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SWC3(YAL011W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWR1(YDR334W)|FD-Score:4.4|P-value:5.44E-6||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TPS3(YMR261C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TUB3(YML124C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:ULA1(YPL003W)|FD-Score:-5.57|P-value:1.28E-8||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VFA1(YER128W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VIP1(YLR410W)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VPS20(YMR077C)|FD-Score:5.37|P-value:4.04E-8||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS3(YDR495C)|FD-Score:4.95|P-value:3.68E-7||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS30(YPL120W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS33(YLR396C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:VPS35(YJL154C)|FD-Score:8.5|P-value:9.31E-18||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS45(YGL095C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS71(YML041C)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:5.34|P-value:4.69E-8||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR221W-A(YBR221W-A_p)|FD-Score:-3.89|P-value:4.97E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR338C(YDR338C_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YDR455C(YDR455C_d)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YFL034W(YFL034W_p)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YIL060W(YIL060W_p)|FD-Score:-5.26|P-value:7.08E-8||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIP5(YGL161C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJR115W(YJR115W_p)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YKR005C(YKR005C_p)|FD-Score:-4.46|P-value:4.16E-6||SGD DESC:Putative protein of unknown function Gene:YLR049C(YLR049C_p)|FD-Score:-3.23|P-value:6.28E-4||SGD DESC:Putative protein of unknown function Gene:YLR118C(YLR118C)|FD-Score:-4.48|P-value:3.72E-6||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR171W(YLR171W_d)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML037C(YML037C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YNL228W(YNL228W_d)|FD-Score:4.44|P-value:4.44E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YNL324W(YNL324W_d)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOS9(YDR057W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPK1(YKL126W)|FD-Score:6.24|P-value:2.20E-10||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR015C(YPR015C_p)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR099C(YPR099C_d)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPT31(YER031C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YTA7(YGR270W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:ADO1(YJR105W)|FD-Score:4.16|P-value:1.60E-5||SGD DESC:Adenosine kinase, required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle Gene:AIM10(YER087W)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:APL2(YKL135C)|FD-Score:3.41|P-value:3.25E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:BDF1(YLR399C)|FD-Score:6.6|P-value:2.07E-11||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:BIO3(YNR058W)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:7,8-diamino-pelargonic acid aminotransferase (DAPA), catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BTS1(YPL069C)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CBF1(YJR060W)|FD-Score:7.2|P-value:2.99E-13||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:12.4|P-value:2.32E-35||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CKB1(YGL019W)|FD-Score:13|P-value:1.09E-38||SGD DESC:Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases Gene:CKB2(YOR039W)|FD-Score:6.08|P-value:5.92E-10||SGD DESC:Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase Gene:DCV1(YFR012W)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication Gene:DOA4(YDR069C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:DRS2(YAL026C)|FD-Score:5.65|P-value:8.12E-9||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DST1(YGL043W)|FD-Score:8.22|P-value:1.00E-16||SGD DESC:General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress Gene:EEB1(YPL095C)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication Gene:ERP2(YAL007C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; member, along with Emp24p and Erv25p, of the p24 family involved in ER to Golgi transport and localized to COPII-coated vesicles Gene:ERV14(YGL054C)|FD-Score:7.01|P-value:1.16E-12||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:GCN5(YGR252W)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GYP1(YOR070C)|FD-Score:6.48|P-value:4.59E-11||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HAP4(YKL109W)|FD-Score:-4.27|P-value:9.73E-6||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:IMP2'(YIL154C)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:ISA2(YPR067W)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations Gene:ISW1(YBR245C)|FD-Score:4.43|P-value:4.69E-6||SGD DESC:ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions Gene:LAA1(YJL207C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene Gene:LDB16(YCL005W)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:MAM3(YOL060C)|FD-Score:3.84|P-value:6.21E-5||SGD DESC:Protein required for normal mitochondrial morphology, has similarity to hemolysins Gene:MAP1(YLR244C)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Methionine aminopeptidase, catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p Gene:MEF1(YLR069C)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MRPL38(YKL170W)|FD-Score:-4.97|P-value:3.40E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:NFT1(YKR103W)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:NMA111(YNL123W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NOP13(YNL175C)|FD-Score:3.78|P-value:7.76E-5||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NOP6(YDL213C)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:NPR1(YNL183C)|FD-Score:4.77|P-value:9.15E-7||SGD DESC:Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex Gene:NTA1(YJR062C)|FD-Score:-4.23|P-value:1.15E-5||SGD DESC:Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation Gene:ODC1(YPL134C)|FD-Score:5.19|P-value:1.03E-7||SGD DESC:Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation Gene:PAF1(YBR279W)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1 Gene:PEP8(YJL053W)|FD-Score:6.42|P-value:6.83E-11||SGD DESC:Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress Gene:PEX1(YKL197C)|FD-Score:-3.21|P-value:6.66E-4||SGD DESC:AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis Gene:PEX30(YLR324W)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome number; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PEX32(YBR168W)|FD-Score:-3.18|P-value:7.43E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PIM1(YBL022C)|FD-Score:3.16|P-value:7.96E-4||SGD DESC:ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria Gene:PRS3(YHL011C)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes Gene:PTC1(YDL006W)|FD-Score:3.15|P-value:8.08E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation Gene:RAM1(YDL090C)|FD-Score:7.19|P-value:3.30E-13||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RCY1(YJL204C)|FD-Score:23.8|P-value:7.33E-126||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RGL1(YPL066W)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:RNQ1(YCL028W)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate Gene:RPP1B(YDL130W)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component Gene:RRT2(YBR246W)|FD-Score:4.17|P-value:1.55E-5||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RSM18(YER050C)|FD-Score:-4.24|P-value:1.13E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:RTT101(YJL047C)|FD-Score:4.8|P-value:8.12E-7||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RXT3(YDL076C)|FD-Score:3.61|P-value:1.54E-4||SGD DESC:Subunit of the RPD3L complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress Gene:SAC1(YKL212W)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:5.45|P-value:2.53E-8||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAF1(YBR280C)|FD-Score:3.77|P-value:8.03E-5||SGD DESC:F-Box protein involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1 Gene:SHR5(YOL110W)|FD-Score:3.15|P-value:8.11E-4||SGD DESC:Subunit of a palmitoyltransferase, composed of Shr5p and Erf2p, that adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization Gene:SKY1(YMR216C)|FD-Score:-3.91|P-value:4.61E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNC2(YOR327C)|FD-Score:-3.28|P-value:5.16E-4||SGD DESC:Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication Gene:SNF7(YLR025W)|FD-Score:4.96|P-value:3.56E-7||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPS2(YDR522C)|FD-Score:-3.13|P-value:8.79E-4||SGD DESC:Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component Gene:SWC3(YAL011W)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWR1(YDR334W)|FD-Score:4.4|P-value:5.44E-6||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TPS3(YMR261C)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication Gene:TUB3(YML124C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:ULA1(YPL003W)|FD-Score:-5.57|P-value:1.28E-8||SGD DESC:Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation Gene:VFA1(YER128W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Protein that interacts with Vps4p and has a role in vacuolar sorting; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology Gene:VIP1(YLR410W)|FD-Score:-3.1|P-value:9.60E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VPS20(YMR077C)|FD-Score:5.37|P-value:4.04E-8||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:VPS3(YDR495C)|FD-Score:4.95|P-value:3.68E-7||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS30(YPL120W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1 Gene:VPS33(YLR396C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole Gene:VPS35(YJL154C)|FD-Score:8.5|P-value:9.31E-18||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:VPS45(YGL095C)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS71(YML041C)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:5.34|P-value:4.69E-8||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR221W-A(YBR221W-A_p)|FD-Score:-3.89|P-value:4.97E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR338C(YDR338C_p)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YDR455C(YDR455C_d)|FD-Score:3.92|P-value:4.34E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YFL034W(YFL034W_p)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YIL060W(YIL060W_p)|FD-Score:-5.26|P-value:7.08E-8||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIP5(YGL161C)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJR115W(YJR115W_p)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YKR005C(YKR005C_p)|FD-Score:-4.46|P-value:4.16E-6||SGD DESC:Putative protein of unknown function Gene:YLR049C(YLR049C_p)|FD-Score:-3.23|P-value:6.28E-4||SGD DESC:Putative protein of unknown function Gene:YLR118C(YLR118C)|FD-Score:-4.48|P-value:3.72E-6||SGD DESC:Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR171W(YLR171W_d)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML037C(YML037C_p)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YNL228W(YNL228W_d)|FD-Score:4.44|P-value:4.44E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1 Gene:YNL324W(YNL324W_d)|FD-Score:5.58|P-value:1.22E-8||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOS9(YDR057W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:ER quality-control lectin; integral subunit of the HRD ligase; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family Gene:YPK1(YKL126W)|FD-Score:6.24|P-value:2.20E-10||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR015C(YPR015C_p)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:Putative protein of unknown function; overexpression causes a cell cycle delay or arrest Gene:YPR099C(YPR099C_d)|FD-Score:-3.27|P-value:5.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL51/YPR100W Gene:YPT31(YER031C)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication Gene:YTA7(YGR270W)|FD-Score:3.34|P-value:4.17E-4||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL048W69.00062.40NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YCR052W6.562.78E-111.09RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YNL267W5.462.33E-80.63PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YMR290W-A_d4.836.72E-70.18YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YDL003W4.651.66E-60.40MCD1Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
YDR394W4.251.09E-50.18RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YMR049C4.072.39E-50.02ERB1Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; homologous to mammalian Bop1
YGL061C4.052.61E-50.01DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YCL054W4.042.71E-50.22SPB1AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
YKR081C3.826.74E-50.00RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YMR290C3.816.82E-50.62HAS1ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
YLR163C3.197.06E-40.04MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YGR128C3.158.03E-40.09UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YOR340C3.070.001080.11RPA43RNA polymerase I subunit A43
YDR141C2.950.001570.02DOP1Golgi-localized, leucine-zipper domain containing protein; involved in endosome to Golgi transport, organization of the ER, establishing cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL204C23.807.33E-126RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YGL019W13.001.09E-38CKB1Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
YCR094W12.402.32E-35CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YJL154C8.509.31E-18VPS35Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
YGL043W8.221.00E-16DST1General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
YJR060W7.202.99E-13CBF1Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress
YDL090C7.193.30E-13RAM1Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
YGL054C7.011.16E-12ERV14COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
YLR399C6.602.07E-11BDF1Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p
YOR070C6.484.59E-11GYP1Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
YJL053W6.426.83E-11PEP8Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
YKL126W6.242.20E-10YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YOR039W6.085.92E-10CKB2Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
YAL026C5.658.12E-9DRS2Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease
YNL324W_d5.581.22E-8YNL324W_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_172
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.7660SGTC_8880916-0002 7.0 μMChemDiv (Drug-like library)28021381NEO1
0.7120SGTC_270tamoxifen 5.5 μMMiscellaneous53760.0588235NEO1
0.7070SGTC_1991st073000 8.9 μMTimTec (Natural product derivative library)43019690.130952NEO1
0.7000SGTC_1665st014639 11.4 μMTimTec (Natural product derivative library)9811330.142857NEO1
0.6750SGTC_5352293-4129 48.2 μMChemDiv (Drug-like library)3961180.0641026NEO1
0.6680SGTC_9001112-0045 141.0 μMChemDiv (Drug-like library)562110.666667NEO1
0.6470SGTC_1797st048695 56.8 μMTimTec (Natural product derivative library)5922620.140845NEO1
0.6330SGTC_1680diphenhydramine 100.0 μMNIH Clinical Collection89800.0877193NEO1
0.6140SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.0672269NEO1
0.6090SGTC_232clozapine 119.0 μMMiscellaneous28180.181818NEO1
0.6060SGTC_1781st038725 66.3 μMTimTec (Natural product derivative library)42702750.140625NEO1
0.6060SGTC_9403232-1287 54.8 μMChemDiv (Drug-like library)15854930.0609756NEO1
0.6010SGTC_3014119-0100 24.5 μMChemDiv (Drug-like library)28010.190476NEO1
0.5950SGTC_11303454-2858 15.9 μMChemDiv (Drug-like library)7156280.171875NEO1
0.5730SGTC_2697mebhydrolin 72.4 μMTimTec (Natural product derivative library)225300.193548NEO1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8880916-00026.97 μM12802138ChemDiv (Drug-like library)377.307485.40312NEO1
SGTC_9001112-0045141 μM0.66666756211ChemDiv (Drug-like library)322.443944.5312NEO1
SGTC_4291112-004925.5 μM0.5714292836170ChemDiv (Drug-like library)473.477668.20212DNA damage response
SGTC_14414106-004013 μM0.562904180ChemDiv (Drug-like library)433.973025.36213
SGTC_12820886-001032.4 μM0.5535712836211ChemDiv (Drug-like library)445.42456.40112endomembrane recycling
SGTC_10430916-000919.3 μM0.5208333101130ChemDiv (Drug-like library)397.295583.40734
SGTC_8870885-00079.62 μM0.4363645719458ChemDiv (Drug-like library)425.565245.16513endomembrane recycling
SGTC_10084106-00376 μM0.406782905394ChemDiv (Drug-like library)443.580525.23214PDR1
SGTC_10094106-00579.87 μM0.406782928005ChemDiv (Drug-like library)443.580525.0314
SGTC_12021988-006829.6 μM0.4032262850480ChemDiv (Drug-like library)426.555.45913RNA processing & uracil transport