st082152

1-[(Z)-2-(1,3-benzodioxol-5-yl)ethenyl]-9H-pyrido[3,4-b]indole

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1721
Screen concentration 9.3 μM
Source TimTec (Natural product derivative library)
PubChem CID 5296051
SMILES C1OC2=C(O1)C=C(C=C2)C=CC3=NC=CC4=C3NC5=CC=CC=C45
Standardized SMILES C1Oc2ccc(C=Cc3[nH]ccc4c5ccccc5nc34)cc2O1
Molecular weight 314.3374
ALogP 4.36
H-bond donor count 1
H-bond acceptor count 3
Response signature 60S ribosome export

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.44
% growth inhibition (Hom. pool) 8.79


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5296051
Download HIP data (tab-delimited text)  (excel)
Gene:ARC35(YNR035C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.06||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:CAB4(YGR277C)|FD-Score:3.12|P-value:9.08E-4|Clearance:0.05||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:CDC43(YGL155W)|FD-Score:-3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:FAP7(YDL166C)|FD-Score:-3.41|P-value:3.27E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:FMN1(YDR236C)|FD-Score:3.17|P-value:7.50E-4|Clearance:0.02||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:HEM13(YDR044W)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) Gene:HYP2(YEL034W)|FD-Score:4.05|P-value:2.52E-5|Clearance:0.01||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:7.99|P-value:6.83E-16|Clearance:1.36||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM5(YER146W)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.16||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:NBP1(YLR457C)|FD-Score:-3.49|P-value:2.38E-4|Clearance:0||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NEO1(YIL048W)|FD-Score:4.71|P-value:1.23E-6|Clearance:0.28||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NMD3(YHR170W)|FD-Score:6.51|P-value:3.78E-11|Clearance:0.95||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP58(YOR310C)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.1||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NSE5(YML023C)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.08||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:POP8(YBL018C)|FD-Score:-4.88|P-value:5.38E-7|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE6(YOL038W)|FD-Score:-3.34|P-value:4.16E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP46(YPL151C)|FD-Score:-3.44|P-value:2.94E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:PRT1(YOR361C)|FD-Score:4.43|P-value:4.72E-6|Clearance:0.22||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RNA15(YGL044C)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.07||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:ROT1(YMR200W)|FD-Score:-4.86|P-value:5.96E-7|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPN11(YFR004W)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:SDH3(YKL141W)|FD-Score:-3.17|P-value:7.68E-4|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SMP3(YOR149C)|FD-Score:5.56|P-value:1.38E-8|Clearance:0.85||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SOF1(YLL011W)|FD-Score:-3.61|P-value:1.53E-4|Clearance:0||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SSY1(YDR160W)|FD-Score:3.77|P-value:8.05E-5|Clearance:0.12||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:SWI1(YPL016W)|FD-Score:-3.15|P-value:8.13E-4|Clearance:0||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TEN1(YLR010C)|FD-Score:4.05|P-value:2.58E-5|Clearance:0.27||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TFC1(YBR123C)|FD-Score:3.15|P-value:8.07E-4|Clearance:0.03||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:UTP20(YBL004W)|FD-Score:4.21|P-value:1.27E-5|Clearance:0.08||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YJU2(YKL095W)|FD-Score:6.62|P-value:1.74E-11|Clearance:0.12||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:YOR282W(YOR282W_d)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C Gene:ARC35(YNR035C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.06||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:CAB4(YGR277C)|FD-Score:3.12|P-value:9.08E-4|Clearance:0.05||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:CDC43(YGL155W)|FD-Score:-3.53|P-value:2.06E-4|Clearance:0||SGD DESC:Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis Gene:FAP7(YDL166C)|FD-Score:-3.41|P-value:3.27E-4|Clearance:0||SGD DESC:Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D Gene:FMN1(YDR236C)|FD-Score:3.17|P-value:7.50E-4|Clearance:0.02||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:HEM13(YDR044W)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p) Gene:HYP2(YEL034W)|FD-Score:4.05|P-value:2.52E-5|Clearance:0.01||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:LSG1(YGL099W)|FD-Score:7.99|P-value:6.83E-16|Clearance:1.36||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:LSM5(YER146W)|FD-Score:3.52|P-value:2.18E-4|Clearance:0.16||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:NBP1(YLR457C)|FD-Score:-3.49|P-value:2.38E-4|Clearance:0||SGD DESC:Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication Gene:NEO1(YIL048W)|FD-Score:4.71|P-value:1.23E-6|Clearance:0.28||SGD DESC:Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus Gene:NMD3(YHR170W)|FD-Score:6.51|P-value:3.78E-11|Clearance:0.95||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NOP58(YOR310C)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.1||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NSE5(YML023C)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.08||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:POP8(YBL018C)|FD-Score:-4.88|P-value:5.38E-7|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRE6(YOL038W)|FD-Score:-3.34|P-value:4.16E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:PRP46(YPL151C)|FD-Score:-3.44|P-value:2.94E-4|Clearance:0||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:PRT1(YOR361C)|FD-Score:4.43|P-value:4.72E-6|Clearance:0.22||SGD DESC:eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes Gene:RNA15(YGL044C)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.07||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:ROT1(YMR200W)|FD-Score:-4.86|P-value:5.96E-7|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPN11(YFR004W)|FD-Score:-3.34|P-value:4.17E-4|Clearance:0||SGD DESC:Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress Gene:SDH3(YKL141W)|FD-Score:-3.17|P-value:7.68E-4|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SMP3(YOR149C)|FD-Score:5.56|P-value:1.38E-8|Clearance:0.85||SGD DESC:Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure Gene:SOF1(YLL011W)|FD-Score:-3.61|P-value:1.53E-4|Clearance:0||SGD DESC:Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p Gene:SSY1(YDR160W)|FD-Score:3.77|P-value:8.05E-5|Clearance:0.12||SGD DESC:Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes Gene:SWI1(YPL016W)|FD-Score:-3.15|P-value:8.13E-4|Clearance:0||SGD DESC:Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer Gene:TEN1(YLR010C)|FD-Score:4.05|P-value:2.58E-5|Clearance:0.27||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TFC1(YBR123C)|FD-Score:3.15|P-value:8.07E-4|Clearance:0.03||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:UTP20(YBL004W)|FD-Score:4.21|P-value:1.27E-5|Clearance:0.08||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:YJU2(YKL095W)|FD-Score:6.62|P-value:1.74E-11|Clearance:0.12||SGD DESC:Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing Gene:YOR282W(YOR282W_d)|FD-Score:3.36|P-value:3.87E-4|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5296051
Download HOP data (tab-delimited text)  (excel)
Gene:AAH1(YNL141W)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:AAP1(YHR047C)|FD-Score:-3.63|P-value:1.39E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:AIM6(YDL237W)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:APE4(YHR113W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:APL5(YPL195W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:BIO2(YGR286C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BIO4(YNR057C)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:CHO2(YGR157W)|FD-Score:-5.58|P-value:1.22E-8||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CHZ1(YER030W)|FD-Score:-3.12|P-value:9.14E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CRP1(YHR146W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CSG2(YBR036C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DIF1(YLR437C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DUS3(YLR401C)|FD-Score:-8.3|P-value:5.07E-17||SGD DESC:Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress Gene:EMC6(YLL014W)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:FMP46(YKR049C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE3(YOR381W)|FD-Score:4.78|P-value:8.58E-7||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FZF1(YGL254W)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GGA1(YDR358W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GLR1(YPL091W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GSC2(YGR032W)|FD-Score:-3.77|P-value:8.03E-5||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GZF3(YJL110C)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HEL2(YDR266C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor Gene:KEL3(YPL263C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:LYS21(YDL131W)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MDM1(YML104C)|FD-Score:-4.87|P-value:5.50E-7||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MNL2(YLR057W)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MTC4(YBR255W)|FD-Score:4.3|P-value:8.45E-6||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NAP1(YKR048C)|FD-Score:-4.08|P-value:2.28E-5||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NFT1(YKR103W)|FD-Score:6.51|P-value:3.79E-11||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:NMD4(YLR363C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NNF2(YGR089W)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:PAU8(YAL068C)|FD-Score:-4.58|P-value:2.38E-6||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions Gene:PCL10(YGL134W)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PDR8(YLR266C)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PEP4(YPL154C)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PET10(YKR046C)|FD-Score:-3.9|P-value:4.85E-5||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PHO80(YOL001W)|FD-Score:13.9|P-value:4.35E-44||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PYC2(YBR218C)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAI1(YGL246C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RCK2(YLR248W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RPA14(YDR156W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPH1(YER169W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RSB1(YOR049C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:RTT10(YPL183C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SDT1(YGL224C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SNA2(YDR525W-A)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SRY1(YKL218C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSO1(YPL232W)|FD-Score:6.99|P-value:1.36E-12||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STP1(YDR463W)|FD-Score:4.27|P-value:9.87E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TBS1(YBR150C)|FD-Score:-4.7|P-value:1.31E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TDH1(YJL052W)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:THI4(YGR144W)|FD-Score:8.85|P-value:4.54E-19||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:THP2(YHR167W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TIR3(YIL011W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TPP1(YMR156C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRF5(YNL299W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TSC3(YBR058C-A)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VIP1(YLR410W)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:YAL037C-A(YAL037C-A_p)|FD-Score:-4.8|P-value:7.99E-7||SGD DESC:Putative protein of unknown function Gene:YBR277C(YBR277C_d)|FD-Score:4.37|P-value:6.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR049C(YCR049C_d)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER152C(YER152C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YER186C(YER186C_p)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Putative protein of unknown function Gene:YFR006W(YFR006W_p)|FD-Score:-3.73|P-value:9.53E-5||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGR122C-A(YGR122C-A_d)|FD-Score:-4.44|P-value:4.54E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YHL012W(YHL012W_p)|FD-Score:-7.21|P-value:2.75E-13||SGD DESC:Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication Gene:YHL041W(YHL041W_d)|FD-Score:6.51|P-value:3.83E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YIL166C(YIL166C_p)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJL182C(YJL182C_d)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YKL118W(YKL118W_d)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR033C(YKR033C_d)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YKR073C(YKR073C_d)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL054C(YLL054C_p)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR030W(YLR030W_p)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Putative protein of unknown function Gene:YLR202C(YLR202C_d)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Putative protein of unknown function Gene:YNL190W(YNL190W_p)|FD-Score:5.05|P-value:2.19E-7||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNR065C(YNR065C_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR012W(YOR012W_p)|FD-Score:-4.72|P-value:1.17E-6||SGD DESC:Putative protein of unknown function Gene:YOR024W(YOR024W_d)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPK1(YKL126W)|FD-Score:10.3|P-value:2.31E-25||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPK3(YBR028C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL197C(YPL197C_d)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YPR077C(YPR077C_d)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPT52(YKR014C)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress Gene:AAH1(YNL141W)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:AAP1(YHR047C)|FD-Score:-3.63|P-value:1.39E-4||SGD DESC:Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication Gene:AIM6(YDL237W)|FD-Score:3.92|P-value:4.37E-5||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:APE4(YHR113W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:APL5(YPL195W)|FD-Score:3.36|P-value:3.87E-4||SGD DESC:Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function Gene:BIO2(YGR286C)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BIO4(YNR057C)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:CHO2(YGR157W)|FD-Score:-5.58|P-value:1.22E-8||SGD DESC:Phosphatidylethanolamine methyltransferase (PEMT), catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis Gene:CHZ1(YER030W)|FD-Score:-3.12|P-value:9.14E-4||SGD DESC:Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress Gene:CRP1(YHR146W)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CSG2(YBR036C)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DIF1(YLR437C)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Protein that regulates the nuclear localization of ribonucleotide reductase Rnr2p and Rnr4p subunits; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half has similarity to S. pombe Spd1 protein Gene:DUS3(YLR401C)|FD-Score:-8.3|P-value:5.07E-17||SGD DESC:Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress Gene:EMC6(YLL014W)|FD-Score:4.05|P-value:2.56E-5||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93 Gene:FMP46(YKR049C)|FD-Score:3.15|P-value:8.18E-4||SGD DESC:Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRE3(YOR381W)|FD-Score:4.78|P-value:8.58E-7||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FZF1(YGL254W)|FD-Score:-3.09|P-value:9.90E-4||SGD DESC:Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress Gene:GGA1(YDR358W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GLR1(YPL091W)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GSC2(YGR032W)|FD-Score:-3.77|P-value:8.03E-5||SGD DESC:Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p) Gene:GZF3(YJL110C)|FD-Score:-3.17|P-value:7.70E-4||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HEL2(YDR266C)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor Gene:KEL3(YPL263C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Cytoplasmic protein of unknown function Gene:LYS21(YDL131W)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication Gene:MDM1(YML104C)|FD-Score:-4.87|P-value:5.50E-7||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MNL2(YLR057W)|FD-Score:4.52|P-value:3.03E-6||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MTC4(YBR255W)|FD-Score:4.3|P-value:8.45E-6||SGD DESC:Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 Gene:NAP1(YKR048C)|FD-Score:-4.08|P-value:2.28E-5||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NFT1(YKR103W)|FD-Score:6.51|P-value:3.79E-11||SGD DESC:Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds. Gene:NMD4(YLR363C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NNF2(YGR089W)|FD-Score:3.84|P-value:6.17E-5||SGD DESC:Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation Gene:PAU8(YAL068C)|FD-Score:-4.58|P-value:2.38E-6||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions Gene:PCL10(YGL134W)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PDR8(YLR266C)|FD-Score:-3.82|P-value:6.73E-5||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PEP4(YPL154C)|FD-Score:3.81|P-value:6.92E-5||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PET10(YKR046C)|FD-Score:-3.9|P-value:4.85E-5||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PHO80(YOL001W)|FD-Score:13.9|P-value:4.35E-44||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PYC2(YBR218C)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication Gene:RAI1(YGL246C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RCK2(YLR248W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RPA14(YDR156W)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPH1(YER169W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNA polymerase II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; RPH1 has a paralog, GIS1, that arose from the whole genome duplication Gene:RPS11A(YDR025W)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication Gene:RRG7(YOR305W)|FD-Score:3.75|P-value:8.91E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RSB1(YOR049C)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:RTT10(YPL183C)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6 Gene:SDT1(YGL224C)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Pyrimidine nucleotidase; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives Gene:SNA2(YDR525W-A)|FD-Score:3.28|P-value:5.17E-4||SGD DESC:Protein of unknown function, has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:SRY1(YKL218C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity Gene:SSO1(YPL232W)|FD-Score:6.99|P-value:1.36E-12||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STP1(YDR463W)|FD-Score:4.27|P-value:9.87E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TBS1(YBR150C)|FD-Score:-4.7|P-value:1.31E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; TBS1 has a paralog, HAL9, that arose from the whole genome duplication Gene:TDH1(YJL052W)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:THI4(YGR144W)|FD-Score:8.85|P-value:4.54E-19||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:THP2(YHR167W)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TIR3(YIL011W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TPP1(YMR156C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRF5(YNL299W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TSC3(YBR058C-A)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:VIP1(YLR410W)|FD-Score:-3.57|P-value:1.76E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:YAL037C-A(YAL037C-A_p)|FD-Score:-4.8|P-value:7.99E-7||SGD DESC:Putative protein of unknown function Gene:YBR277C(YBR277C_d)|FD-Score:4.37|P-value:6.30E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W Gene:YCR049C(YCR049C_d)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER038W-A(YER038W-A_d)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER152C(YER152C)|FD-Score:3.17|P-value:7.54E-4||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YER186C(YER186C_p)|FD-Score:-3.17|P-value:7.67E-4||SGD DESC:Putative protein of unknown function Gene:YFR006W(YFR006W_p)|FD-Score:-3.73|P-value:9.53E-5||SGD DESC:Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene Gene:YGR122C-A(YGR122C-A_d)|FD-Score:-4.44|P-value:4.54E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, similar to YLR334C and YOL106W Gene:YHL012W(YHL012W_p)|FD-Score:-7.21|P-value:2.75E-13||SGD DESC:Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication Gene:YHL041W(YHL041W_d)|FD-Score:6.51|P-value:3.83E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YIL166C(YIL166C_p)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Putative protein with similarity to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; YIL166C is a non-essential gene Gene:YJL182C(YJL182C_d)|FD-Score:3.92|P-value:4.36E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YKL118W(YKL118W_d)|FD-Score:4.58|P-value:2.33E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKR033C(YKR033C_d)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YKR073C(YKR073C_d)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLL054C(YLL054C_p)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene Gene:YLR030W(YLR030W_p)|FD-Score:5.09|P-value:1.76E-7||SGD DESC:Putative protein of unknown function Gene:YLR202C(YLR202C_d)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Putative protein of unknown function Gene:YNL190W(YNL190W_p)|FD-Score:5.05|P-value:2.19E-7||SGD DESC:Hydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site Gene:YNR065C(YNR065C_p)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene Gene:YOR012W(YOR012W_p)|FD-Score:-4.72|P-value:1.17E-6||SGD DESC:Putative protein of unknown function Gene:YOR024W(YOR024W_d)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR072W(YOR072W_d)|FD-Score:3.92|P-value:4.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPK1(YKL126W)|FD-Score:10.3|P-value:2.31E-25||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPK3(YBR028C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner Gene:YPL197C(YPL197C_d)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the ribosomal gene RPB7B Gene:YPR077C(YPR077C_d)|FD-Score:3.76|P-value:8.51E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1 Gene:YPT52(YKR014C)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL099W7.996.83E-161.36LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YKL095W6.621.74E-110.12YJU2Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC (nineteen complex) and acts after Prp2p to promote the first catalytic reaction of splicing
YHR170W6.513.78E-110.95NMD3Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
YOR149C5.561.38E-80.84SMP3Alpha 1,2-mannosyltransferase involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
YIL048W4.711.23E-60.28NEO1Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
YOR361C4.434.72E-60.22PRT1eIF3b subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes
YBL004W4.211.27E-50.08UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YGL044C4.131.83E-50.07RNA15Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YEL034W4.052.52E-50.01HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YLR010C4.052.58E-50.27TEN1Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
YDR160W3.778.05E-50.12SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YNR035C3.651.29E-40.06ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YML023C3.591.63E-40.08NSE5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YER146W3.522.18E-40.15LSM5Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
YOR282W_d3.363.87E-40.08YOR282W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOL001W13.904.35E-44PHO80Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
YKL126W10.302.31E-25YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YGR144W8.854.54E-19THI4Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
YPL232W6.991.36E-12SSO1Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
YKR103W6.513.79E-11NFT1Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds.
YHL041W_d6.513.83E-11YHL041W_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
YLR030W_p5.091.76E-7YLR030W_pPutative protein of unknown function
YJL052W5.072.00E-7TDH1Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
YNL190W_p5.052.19E-7YNL190W_pHydrophilin essential in dessication-rehydration process; cell wall protein; contains a putative GPI-attachment site
YOR381W4.788.58E-7FRE3Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
YKL118W_d4.582.33E-6YKL118W_dDubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2
YLR057W4.523.03E-6MNL2Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
YBR277C_d4.376.30E-6YBR277C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
YBR255W4.308.45E-6MTC4Protein of unknown function, required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1
YDR463W4.279.87E-6STP1Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_1721
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1667.83E-38SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0777778
0.1591.73E-34SGTC_1573oleylamine 1.1 μMTimTec (Pure natural product library)53567890.016129
0.1478.28E-30SGTC_30229087993 49.5 μMChembridge (Drug-like library)171736440.0714286
0.1271.21E-22SGTC_14033909-7815 62.9 μMChemDiv (Drug-like library)66154830.109756
0.1233.32E-21SGTC_7651319-0117 244.0 μMChemDiv (Drug-like library)34877460.12087960S ribosome export
0.1181.23E-19SGTC_1070indatraline 8.8 μMNIH Clinical Collection103144720.160S ribosome export
0.1171.63E-19SGTC_2740felodipine 17.1 μMMiscellaneous33330.0481928
0.1162.94E-19SGTC_1913914-0123 66.5 μMChemDiv (Drug-like library)31444120.10389660S ribosome export
0.1163.44E-19SGTC_2658polymyxin b sulfate 77.0 μMMicrosource (Natural product library)57021050.046875NEO1
0.1151.30E-18SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.0752688
0.1141.31E-18SGTC_618k072-0232 33.2 μMChemDiv (Drug-like library)58727560.13888960S ribosome export
0.1142.09E-18SGTC_1958st076513 41.9 μMTimTec (Natural product derivative library)7321220.17647160S ribosome export
0.1124.59E-18SGTC_6450845-0943 5.4 μMChemDiv (Drug-like library)18851060.070588260S ribosome export
0.1124.73E-18SGTC_31859108267 49.5 μMChembridge (Drug-like library)49104860.064516160S ribosome export
0.1102.30E-17SGTC_4253825-7017 13.1 μMChemDiv (Drug-like library)33974400.118421

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2716st08085838.62 μM0.5849065296050TimTec (Natural product derivative library)295.337324.4661260S ribosome export
SGTC_1516st013856110 μM0.425281404TimTec (Pure natural product library)182.22122.45511
SGTC_14404100-155040 μM0.409836742806ChemDiv (Drug-like library)268.267382.23914
SGTC_7991310-0557151 μM0.3928575354492ChemDiv (Drug-like library)252.264683.4703superoxide
SGTC_1843st05580945.5 μM0.35384616413835TimTec (Natural product derivative library)437.314532.60903
SGTC_2587piperic acid92.2 μM0.3508775370536Microsource (Natural product library)218.20542.16314
SGTC_1594piperine70.1 μM0.31746638024TimTec (Pure natural product library)285.337662.86403
SGTC_2683piperine63.03 μM0.31746638024TimTec (Pure natural product library)285.337662.86403
SGTC_13661683-011321.9 μM0.310345708623ChemDiv (Drug-like library)224.233121.75925
SGTC_1845st0567533.01 μM0.3050855375839TimTec (Natural product derivative library)207.182762.09104TSC3-RPN4
SGTC_1795st04954562.3 μM0.3015875291882TimTec (Natural product derivative library)321.15484.79803TSC3-RPN4