st034307

6-chloro-2-(trichloromethyl)chromen-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_1723
Screen concentration 2.5 μM
Source TimTec (Natural product derivative library)
PubChem CID 867466
SMILES C1=CC2=C(C=C1Cl)C(=O)C=C(O2)C(Cl)(Cl)Cl
Standardized SMILES Clc1ccc2OC(=CC(=O)c2c1)C(Cl)(Cl)Cl
Molecular weight 297.9496
ALogP 3.81
H-bond donor count 0
H-bond acceptor count 2
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.83
% growth inhibition (Hom. pool) 4.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 867466
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.16||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP7(YPR034W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.05||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ASA1(YPR085C)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.05||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:BIG1(YHR101C)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.02||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:BRN1(YBL097W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.04||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CBF2(YGR140W)|FD-Score:6.63|P-value:1.67E-11|Clearance:0.34||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC34(YDR054C)|FD-Score:-3.11|P-value:9.33E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:DAM1(YGR113W)|FD-Score:-3.11|P-value:9.25E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:EPL1(YFL024C)|FD-Score:-3.58|P-value:1.74E-4|Clearance:0||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:FIP1(YJR093C)|FD-Score:4.02|P-value:2.87E-5|Clearance:0.27||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:HYP2(YEL034W)|FD-Score:5.94|P-value:1.44E-9|Clearance:0.04||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IRA1(YBR140C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.04||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM2(YBL026W)|FD-Score:5.9|P-value:1.85E-9|Clearance:0.57||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NDC1(YML031W)|FD-Score:6.29|P-value:1.55E-10|Clearance:0.02||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NMD3(YHR170W)|FD-Score:3.14|P-value:8.54E-4|Clearance:0.07||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSE1(YLR007W)|FD-Score:3.76|P-value:8.63E-5|Clearance:0.05||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PDC2(YDR081C)|FD-Score:5.05|P-value:2.21E-7|Clearance:0.25||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PET9(YBL030C)|FD-Score:3.36|P-value:3.88E-4|Clearance:0.01||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:PRP42(YDR235W)|FD-Score:-3.72|P-value:1.00E-4|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:RBA50(YDR527W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.02||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RER2(YBR002C)|FD-Score:7.02|P-value:1.15E-12|Clearance:0.38||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIB3(YDR487C)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RIX1(YHR197W)|FD-Score:-4.26|P-value:1.02E-5|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RNA15(YGL044C)|FD-Score:-3.97|P-value:3.63E-5|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB2(YOR151C)|FD-Score:5.33|P-value:4.96E-8|Clearance:0.28||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPB7(YDR404C)|FD-Score:4.8|P-value:8.01E-7|Clearance:0.21||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL25(YOL127W)|FD-Score:4.59|P-value:2.26E-6|Clearance:0.29||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPT3(YDR394W)|FD-Score:6.28|P-value:1.72E-10|Clearance:0.34||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:4.28|P-value:9.41E-6|Clearance:0.04||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSC58(YLR033W)|FD-Score:10.1|P-value:2.08E-24|Clearance:3.11||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:4.3|P-value:8.57E-6|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RVB2(YPL235W)|FD-Score:-3.95|P-value:3.84E-5|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC16(YPL085W)|FD-Score:-4.11|P-value:1.98E-5|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SMX2(YFL017W-A)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.06||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:TOR2(YKL203C)|FD-Score:-3.74|P-value:9.06E-5|Clearance:0||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:UTP9(YHR196W)|FD-Score:-4.41|P-value:5.11E-6|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGL102C(YGL102C_d)|FD-Score:3.35|P-value:4.00E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein Gene:ACC1(YNR016C)|FD-Score:4.18|P-value:1.43E-5|Clearance:0.16||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP7(YPR034W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.05||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ASA1(YPR085C)|FD-Score:4.24|P-value:1.14E-5|Clearance:0.05||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:BIG1(YHR101C)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.02||SGD DESC:Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan Gene:BRN1(YBL097W)|FD-Score:3.48|P-value:2.54E-4|Clearance:0.04||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:CBF2(YGR140W)|FD-Score:6.63|P-value:1.67E-11|Clearance:0.34||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CDC34(YDR054C)|FD-Score:-3.11|P-value:9.33E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:DAM1(YGR113W)|FD-Score:-3.11|P-value:9.25E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments Gene:EPL1(YFL024C)|FD-Score:-3.58|P-value:1.74E-4|Clearance:0||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:FIP1(YJR093C)|FD-Score:4.02|P-value:2.87E-5|Clearance:0.27||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:HYP2(YEL034W)|FD-Score:5.94|P-value:1.44E-9|Clearance:0.04||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IRA1(YBR140C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.04||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM2(YBL026W)|FD-Score:5.9|P-value:1.85E-9|Clearance:0.57||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:NDC1(YML031W)|FD-Score:6.29|P-value:1.55E-10|Clearance:0.02||SGD DESC:Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 Gene:NMD3(YHR170W)|FD-Score:3.14|P-value:8.54E-4|Clearance:0.07||SGD DESC:Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex Gene:NSE1(YLR007W)|FD-Score:3.76|P-value:8.63E-5|Clearance:0.05||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:PDC2(YDR081C)|FD-Score:5.05|P-value:2.21E-7|Clearance:0.25||SGD DESC:Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions Gene:PET9(YBL030C)|FD-Score:3.36|P-value:3.88E-4|Clearance:0.01||SGD DESC:Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication Gene:PRP42(YDR235W)|FD-Score:-3.72|P-value:1.00E-4|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:RBA50(YDR527W)|FD-Score:3.4|P-value:3.42E-4|Clearance:0.02||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RER2(YBR002C)|FD-Score:7.02|P-value:1.15E-12|Clearance:0.38||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIB3(YDR487C)|FD-Score:-3.59|P-value:1.68E-4|Clearance:0||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RIX1(YHR197W)|FD-Score:-4.26|P-value:1.02E-5|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:RNA15(YGL044C)|FD-Score:-3.97|P-value:3.63E-5|Clearance:0||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPB2(YOR151C)|FD-Score:5.33|P-value:4.96E-8|Clearance:0.28||SGD DESC:RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit Gene:RPB7(YDR404C)|FD-Score:4.8|P-value:8.01E-7|Clearance:0.21||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPL25(YOL127W)|FD-Score:4.59|P-value:2.26E-6|Clearance:0.29||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPL32(YBL092W)|FD-Score:3.71|P-value:1.03E-4|Clearance:0.18||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPT3(YDR394W)|FD-Score:6.28|P-value:1.72E-10|Clearance:0.34||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:4.28|P-value:9.41E-6|Clearance:0.04||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSC58(YLR033W)|FD-Score:10.1|P-value:2.08E-24|Clearance:3.11||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:4.3|P-value:8.57E-6|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RVB2(YPL235W)|FD-Score:-3.95|P-value:3.84E-5|Clearance:0||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC16(YPL085W)|FD-Score:-4.11|P-value:1.98E-5|Clearance:0||SGD DESC:COPII vesicle coat protein required for ER transport vesicle budding; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p Gene:SMX2(YFL017W-A)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.06||SGD DESC:Core Sm protein Sm G; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx3p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm G Gene:TOR2(YKL203C)|FD-Score:-3.74|P-value:9.06E-5|Clearance:0||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication Gene:UTP9(YHR196W)|FD-Score:-4.41|P-value:5.11E-6|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YGL102C(YGL102C_d)|FD-Score:3.35|P-value:4.00E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential RPL28 gene encoding a large subunit ribosomal protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 867466
Download HOP data (tab-delimited text)  (excel)
Gene:AKR1(YDR264C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARG4(YHR018C)|FD-Score:-4.67|P-value:1.48E-6||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:ATP14(YLR295C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BTS1(YPL069C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCM1(YGR150C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CLC1(YGR167W)|FD-Score:7.38|P-value:7.88E-14||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG6(YNL041C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:5.83|P-value:2.73E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:6.02|P-value:8.61E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSF1(YLR087C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DCS1(YLR270W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DIA3(YDL024C)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:ECM25(YJL201W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:FCY1(YPR062W)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FYV5(YCL058C)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GEP4(YHR100C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLG2(YJL137C)|FD-Score:-4.53|P-value:2.91E-6||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GPM3(YOL056W)|FD-Score:-3.94|P-value:4.05E-5||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HCH1(YNL281W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HHF1(YBR009C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HOM6(YJR139C)|FD-Score:5.51|P-value:1.77E-8||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOP2(YGL033W)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:ICY1(YMR195W)|FD-Score:5.8|P-value:3.33E-9||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IME1(YJR094C)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:IRC13(YOR235W_d)|FD-Score:4.86|P-value:5.78E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:JID1(YPR061C)|FD-Score:4.54|P-value:2.79E-6||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:JLP1(YLL057C)|FD-Score:-4.75|P-value:1.04E-6||SGD DESC:Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation Gene:KNS1(YLL019C)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LDB18(YLL049W)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:LIN1(YHR156C)|FD-Score:4.99|P-value:3.08E-7||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:LIP2(YLR239C)|FD-Score:5.12|P-value:1.49E-7||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MCH2(YKL221W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDJ1(YFL016C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MDM1(YML104C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MPP6(YNR024W)|FD-Score:-6.1|P-value:5.17E-10||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPL1(YDR116C)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL27(YBR282W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL7(YDR237W)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:NCL1(YBL024W)|FD-Score:-5.41|P-value:3.20E-8||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:PEP7(YDR323C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET10(YKR046C)|FD-Score:-6.86|P-value:3.51E-12||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX4(YGR133W)|FD-Score:-6.44|P-value:5.91E-11||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:PKP2(YGL059W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PMR1(YGL167C)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:POT1(YIL160C)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PTR2(YKR093W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:QCR7(YDR529C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RAD54(YGL163C)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAI1(YGL246C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RBS1(YDL189W)|FD-Score:-3.72|P-value:9.95E-5||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:RHR2(YIL053W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RNR4(YGR180C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ROG1(YGL144C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication Gene:RPA14(YDR156W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPL19A(YBR084C-A)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.31|P-value:8.23E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSM18(YER050C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:SLM5(YCR024C)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SPR6(YER115C)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SPT8(YLR055C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRC1(YML034W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSN3(YPL042C)|FD-Score:5.72|P-value:5.17E-9||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SUV3(YPL029W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWC5(YBR231C)|FD-Score:8.54|P-value:6.54E-18||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TCB1(YOR086C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TDH1(YJL052W)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TIM13(YGR181W)|FD-Score:-5.05|P-value:2.18E-7||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TUF1(YOR187W)|FD-Score:4.68|P-value:1.47E-6||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:VPS1(YKR001C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VTS1(YOR359W)|FD-Score:-3.73|P-value:9.71E-5||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YBR062C(YBR062C_p)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR287W(YBR287W_p)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene Gene:YCK3(YER123W)|FD-Score:4.49|P-value:3.64E-6||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR417C(YDR417C_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YEL043W(YEL043W)|FD-Score:6.21|P-value:2.67E-10||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YER053C-A(YER053C-A_p)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YGR067C(YGR067C_p)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p Gene:YIL108W(YIL108W_p)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YKL068W-A(YKL068W-A_p)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR042C(YLR042C_p)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR413W(YLR413W_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YNL226W(YNL226W_d)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YNR014W(YNR014W_p)|FD-Score:4|P-value:3.17E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YOL029C(YOL029C_p)|FD-Score:5.34|P-value:4.55E-8||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YPR092W(YPR092W_d)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AKR1(YDR264C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication Gene:ARG4(YHR018C)|FD-Score:-4.67|P-value:1.48E-6||SGD DESC:Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway Gene:ATP14(YLR295C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:BTS1(YPL069C)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCM1(YGR150C)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CLC1(YGR167W)|FD-Score:7.38|P-value:7.88E-14||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COG6(YNL041C)|FD-Score:3.33|P-value:4.30E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:5.83|P-value:2.73E-9||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:6.02|P-value:8.61E-10||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CSF1(YLR087C)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DCS1(YLR270W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DIA3(YDL024C)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth Gene:ECM25(YJL201W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:FCY1(YPR062W)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU) Gene:FYV5(YCL058C)|FD-Score:-4.06|P-value:2.49E-5||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GEP4(YHR100C)|FD-Score:4.2|P-value:1.36E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GLG2(YJL137C)|FD-Score:-4.53|P-value:2.91E-6||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:GPM3(YOL056W)|FD-Score:-3.94|P-value:4.05E-5||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HCH1(YNL281W)|FD-Score:-3.11|P-value:9.49E-4||SGD DESC:Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress Gene:HHF1(YBR009C)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HOM6(YJR139C)|FD-Score:5.51|P-value:1.77E-8||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HOP2(YGL033W)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:ICY1(YMR195W)|FD-Score:5.8|P-value:3.33E-9||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IME1(YJR094C)|FD-Score:-3.14|P-value:8.39E-4||SGD DESC:Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p Gene:IRC13(YOR235W_d)|FD-Score:4.86|P-value:5.78E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:JID1(YPR061C)|FD-Score:4.54|P-value:2.79E-6||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:JLP1(YLL057C)|FD-Score:-4.75|P-value:1.04E-6||SGD DESC:Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase, involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation Gene:KNS1(YLL019C)|FD-Score:-3.12|P-value:9.04E-4||SGD DESC:Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation Gene:LDB18(YLL049W)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24 Gene:LIN1(YHR156C)|FD-Score:4.99|P-value:3.08E-7||SGD DESC:Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication Gene:LIP2(YLR239C)|FD-Score:5.12|P-value:1.49E-7||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MCH2(YKL221W)|FD-Score:3.13|P-value:8.83E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MDJ1(YFL016C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Co-chaperone that stimulates the ATPase activity of the HSP70 protein Ssc1p; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones Gene:MDM1(YML104C)|FD-Score:-3.47|P-value:2.58E-4||SGD DESC:Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds; contains a Phox homology (PX) domain and specifically binds phosphatidylinositol 3-phosphate (PtdIns-3-P) Gene:MPP6(YNR024W)|FD-Score:-6.1|P-value:5.17E-10||SGD DESC:Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes Gene:MRPL1(YDR116C)|FD-Score:3.85|P-value:5.84E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL27(YBR282W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP Gene:MRPL7(YDR237W)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein Gene:NCL1(YBL024W)|FD-Score:-5.41|P-value:3.20E-8||SGD DESC:S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120 Gene:PEP7(YDR323C)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PET10(YKR046C)|FD-Score:-6.86|P-value:3.51E-12||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX4(YGR133W)|FD-Score:-6.44|P-value:5.91E-11||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:PKP2(YGL059W)|FD-Score:-3.66|P-value:1.28E-4||SGD DESC:Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:PMR1(YGL167C)|FD-Score:4.65|P-value:1.63E-6||SGD DESC:High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease Gene:POT1(YIL160C)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids Gene:PTR2(YKR093W)|FD-Score:3.32|P-value:4.45E-4||SGD DESC:Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p Gene:QCR7(YDR529C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly Gene:RAD54(YGL163C)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RAI1(YGL246C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z Gene:RBS1(YDL189W)|FD-Score:-3.72|P-value:9.95E-5||SGD DESC:Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain Gene:RHR2(YIL053W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RNR4(YGR180C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:ROG1(YGL144C)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Protein with putative serine active lipase domain; ROG1 has a paralog, YDL109C, that arose from the whole genome duplication Gene:RPA14(YDR156W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:RNA polymerase I subunit A14 Gene:RPL19A(YBR084C-A)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.31|P-value:8.23E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSM18(YER050C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:SLM5(YCR024C)|FD-Score:3.11|P-value:9.43E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SPR6(YER115C)|FD-Score:-3.6|P-value:1.59E-4||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:SPT8(YLR055C)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters Gene:SRC1(YML034W)|FD-Score:3.59|P-value:1.63E-4||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSN3(YPL042C)|FD-Score:5.72|P-value:5.17E-9||SGD DESC:Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression Gene:SUV3(YPL029W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWC5(YBR231C)|FD-Score:8.54|P-value:6.54E-18||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:3.92|P-value:4.48E-5||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TCB1(YOR086C)|FD-Score:-3.33|P-value:4.32E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TDH1(YJL052W)|FD-Score:-3.85|P-value:5.81E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TIM13(YGR181W)|FD-Score:-5.05|P-value:2.18E-7||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TUF1(YOR187W)|FD-Score:4.68|P-value:1.47E-6||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:VPS1(YKR001C)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VTS1(YOR359W)|FD-Score:-3.73|P-value:9.71E-5||SGD DESC:Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity Gene:YBR062C(YBR062C_p)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway. Gene:YBR287W(YBR287W_p)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene Gene:YCK3(YER123W)|FD-Score:4.49|P-value:3.64E-6||SGD DESC:Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway Gene:YDR417C(YDR417C_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W Gene:YEL043W(YEL043W)|FD-Score:6.21|P-value:2.67E-10||SGD DESC:Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold Gene:YER053C-A(YER053C-A_p)|FD-Score:4.02|P-value:2.91E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YGR067C(YGR067C_p)|FD-Score:-3.2|P-value:6.88E-4||SGD DESC:Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p Gene:YIL108W(YIL108W_p)|FD-Score:-3.09|P-value:9.95E-4||SGD DESC:Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress Gene:YKL068W-A(YKL068W-A_p)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR042C(YLR042C_p)|FD-Score:3.17|P-value:7.61E-4||SGD DESC:Protein of unknown function; localizes to the cytoplasm; YLL042C is not an essential gene Gene:YLR413W(YLR413W_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Putative protein of unknown function; YLR413W is not an essential gene Gene:YNL226W(YNL226W_d)|FD-Score:4.01|P-value:3.01E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C Gene:YNR014W(YNR014W_p)|FD-Score:4|P-value:3.17E-5||SGD DESC:Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication Gene:YOL029C(YOL029C_p)|FD-Score:5.34|P-value:4.55E-8||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YPR092W(YPR092W_d)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W10.102.08E-243.11RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YBR002C7.021.15E-120.39RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YGR140W6.631.67E-110.34CBF2Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
YML031W6.291.55E-100.02NDC1Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
YDR394W6.281.72E-100.34RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YEL034W5.941.44E-90.04HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YBL026W5.901.85E-90.57LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YOR151C5.334.96E-80.28RPB2RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit
YDR081C5.052.21E-70.25PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YDR404C4.808.01E-70.21RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YOL127W4.592.26E-60.29RPL25Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23
YFR037C4.308.57E-60.02RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YOR117W4.289.41E-60.04RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YPR085C4.241.14E-50.05ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YNR016C4.181.43E-50.16ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR231C8.546.54E-18SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YGR167W7.387.88E-14CLC1Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
YEL043W6.212.67E-10YEL043WPredicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold
YML071C6.028.61E-10COG8Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YGL005C5.832.73E-9COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YMR195W5.803.33E-9ICY1Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation
YPR062W5.783.70E-9FCY1Cytosine deaminase, zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
YPL042C5.725.17E-9SSN3Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
YJR139C5.511.77E-8HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YOL029C_p5.344.55E-8YOL029C_pPutative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens
YLR239C5.121.49E-7LIP2Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
YHR156C4.993.08E-7LIN1Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
YOR235W_d4.865.78E-7IRC13_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci
YDR237W4.751.03E-6MRPL7Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
YOR187W4.681.47E-6TUF1Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans

GO enrichment analysis for SGTC_1723
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2035.04E-56SGTC_1722st034304 3.7 μMTimTec (Natural product derivative library)40361480.628571mitochondrial processes
0.1958.62E-52SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0952381RSC complex & mRNA processing
0.1781.77E-43SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.0980392RSC complex & mRNA processing
0.1755.26E-42SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.09375RSC & ERG11
0.1758.22E-42SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.147541RSC complex & mRNA processing
0.1731.13E-40SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.109091RSC complex & mRNA processing
0.1673.05E-38SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.078125RSC complex & mRNA processing
0.1674.24E-38SGTC_7830848-0141 92.7 μMChemDiv (Drug-like library)67550760.0441176RSC & ERG11
0.1645.88E-37SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.142857RSC complex & mRNA processing
0.1648.23E-37SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.126984RSC complex & mRNA processing
0.1648.39E-37SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.037037RSC complex & mRNA processing
0.1561.28E-33SGTC_624k048-0052 28.0 μMChemDiv (Drug-like library)68355310.0746269Golgi
0.1454.22E-29SGTC_6731082-0474 110.0 μMChemDiv (Drug-like library)30980460.12069RSC complex & mRNA processing
0.1454.40E-29SGTC_32779138724 49.5 μMChembridge (Drug-like library)159434180.0517241Golgi
0.1411.84E-27SGTC_930109-0045 59.2 μMChemDiv (Drug-like library)887330.0754717DNA damage response

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1732st03430851.82 μM0.694444830119TimTec (Natural product derivative library)277.531083.63602
SGTC_1727st0343099.9 μM0.641026870305TimTec (Natural product derivative library)293.530483.13403
SGTC_1722st0343043.65 μM0.6285714036148TimTec (Natural product derivative library)263.50453.1502mitochondrial processes
SGTC_1735st03431056.75 μM0.52381871153TimTec (Natural product derivative library)293.530483.13403Golgi
SGTC_1896st05844189 μM0.422222688759TimTec (Natural product derivative library)224.640362.603
SGTC_1780st03844824.54 μM0.36728868TimTec (Natural product derivative library)278.710883.21614iron homeostasis
SGTC_15526-methylflavone10.6 μM0.347826689013TimTec (Pure natural product library)236.265283.62202superoxide
SGTC_1766st04541444.77 μM0.346154253960TimTec (Natural product derivative library)273.671222.11214RPP1 & pyrimidine depletion
SGTC_18943',6'-dihydroxyflavone78.7 μM0.340426688662TimTec (Natural product derivative library)254.23752.65224
SGTC_1866st05763864 μM0.326531688802TimTec (Natural product derivative library)312.316643.08705